scholarly journals Radiation-induced damage in E. coli B: The effect of superoxide radicals and molecular oxygen. Progress report, December 1, 1978--November 30, 1979

1979 ◽  
Author(s):  
A Samuni ◽  
G Czapski
Keyword(s):  
Molecular Oxygen ◽  
Progress Report ◽  
Superoxide Radicals ◽  
E Coli ◽  
Radiation Induced

Radiation-Induced Damage in T4 Bacteriophage: The Effect of Superoxide Radicals and Molecular Oxygen

1978 ◽  
Vol 75 (3) ◽  
pp. 489 ◽  
Author(s):  
Amram Samuni ◽  
Mordechai Chevion ◽  
Yeheskel S. Halpern ◽  
Yael A. Ilan ◽  
Gidon Czapski
Keyword(s):  
Molecular Oxygen ◽  
Superoxide Radicals ◽  
T4 Bacteriophage ◽  
Radiation Induced

Developing a Genetic System in Deinococcus radiodurans for Analyzing Mutations

Genetics ◽  
2004 ◽  
Vol 166 (2) ◽  
pp. 661-668
Author(s):  
Mandy Kim ◽  
Erika Wolff ◽  
Tiffany Huang ◽  
Lilit Garibyan ◽  
Ashlee M Earl ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Deinococcus Radiodurans ◽  
Genetic System ◽  
Base Change ◽  
Base Substitution ◽  
Wild Type ◽  
Base Pairs ◽  
E Coli ◽  
Radiation Induced ◽  
Induced Mutagenesis

Abstract We have applied a genetic system for analyzing mutations in Escherichia coli to Deinococcus radiodurans, an extremeophile with an astonishingly high resistance to UV- and ionizing-radiation-induced mutagenesis. Taking advantage of the conservation of the β-subunit of RNA polymerase among most prokaryotes, we derived again in D. radiodurans the rpoB/Rif r system that we developed in E. coli to monitor base substitutions, defining 33 base change substitutions at 22 different base pairs. We sequenced >250 mutations leading to Rif r in D. radiodurans derived spontaneously in wild-type and uvrD (mismatch-repair-deficient) backgrounds and after treatment with N-methyl-N′-nitro-N-nitrosoguanidine (NTG) and 5-azacytidine (5AZ). The specificities of NTG and 5AZ in D. radiodurans are the same as those found for E. coli and other organisms. There are prominent base substitution hotspots in rpoB in both D. radiodurans and E. coli. In several cases these are at different points in each organism, even though the DNA sequences surrounding the hotspots and their corresponding sites are very similar in both D. radiodurans and E. coli. In one case the hotspots occur at the same site in both organisms.

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