scholarly journals In vitro mutagenicity testing. II. Silastic 386 Foam Elastomer, Irganox 1010, mixture of Sylgard 184 with Encapsulating Resin and Curing Agent, and dimethylbenzanthracene. [Ames test]

1980 ◽  
Author(s):  
S Wang ◽  
D Smith
Keyword(s):  
Ames Test ◽  
Curing Agent ◽  
Irganox 1010 ◽  
Mutagenicity Testing

scholarly journals Mutagenicity testing of ST-film with the Ames test, chromosome test in vitro and micronucleus test in female mice.

1985 ◽  
Vol 10 (2) ◽  
pp. 135-141 ◽  
Author(s):  
Tohru SHIBUYA ◽  
Noriho TANAKA ◽  
Motoe KATOH ◽  
Yoshie MATSUDA ◽  
Ken'ichi MORITA
Keyword(s):  
Ames Test ◽  
Micronucleus Test ◽  
Mutagenicity Testing ◽  
Female Mice

In Vitro Mutagenicity Testing of WR6026 Hydrochloride

1994 ◽  
Author(s):  
Barry S. Levine ◽  
Richard H. San ◽  
Patrick D. Curry
Keyword(s):  
Mutagenicity Testing

Effects of melatonin on N-nitroso-N-methylurea-induced carcinogenesis in rats and mutagenesis in vitro (Ames test and COMET assay)

1999 ◽  
Vol 138 (1-2) ◽  
pp. 37-44 ◽  
Author(s):  
Sergei A Musatov ◽  
Vladimir N Anisimov ◽  
Véronique André ◽  
Carole Vigreux ◽  
Thierry Godard ◽  
...  
Keyword(s):  
Comet Assay ◽  
Ames Test

scholarly journals Evaluation of mutagenicity, genotoxicity and chronic toxicity of antiviral drug imidazolyl ethanamide pentandioic acid in in vitro and in vivo test systems

2021 ◽  
Vol 98 (5) ◽  
pp. 548-557
Author(s):  
E. A. Jain ◽  
D. Pleimes ◽  
A. A. Globenko
Keyword(s):  
Oral Administration ◽  
Chromosomal Aberrations ◽  
Ames Test ◽  
Chronic Toxicity ◽  
Micronucleus Test ◽  
Human Lymphocytes ◽  
Antiviral Drug ◽  
Systemic Exposure

Introduction. The antiviral properties of imidazolyl ethanamide pentandioic acid (IPA), the active compound of the drug product, has been proven in various experimental models. However, the literature data on the toxicological properties of IPA are limited.Purpose. To evaluate mutagenic and genotoxic properties in in vitro and in vivo models, as well as to study the toxicity of IPA following chronic oral administration to rats and dogs.Materials and methods. Mutagenic and genotoxic properties of IPA were assessed using the Ames test, the test of chromosomal aberrations in human lymphocytes, and the micronucleus test in rats. The chronic toxicity of IPA was studied in Sprague Dawley rats and beagle dogs of both sexes, to which IPA was administered orally at doses of 30-300 mg/kg/day for 26 and 39 weeks, respectively.Results and discussion. In the Ames test, the addition of IPA up to the maximum dose (5000 mcg/plate) did not result in the increase in the number of revertant colonies. At a concentration of up to 5000 mcg/ml, IPA did not cause chromosomal aberrations in human leukocytes. At doses doses ≤ 2000 mg/kg, IPA did not increase the amount of micronuclei in the bone marrow of rats. In chronic experiments, animals tolerated the administration of IPA well: the dose without an observed effect (NOEL) for rats and dogs was 300 mg/kg/day.Conclusion. IPA did not show mutagenic and genotoxic properties in standard in vitro and in vivo tests. With chronic oral administration to rats and dogs, NOEL IPA equal to 300 mg/kg/day provided a systemic exposure that was 8-10 and 41-65 times higher than that in humans, respectively. The results obtained allow us to consider the safety profile of the prolonged use in humans as favorable.

scholarly journals In vitro toxicity studies of novel solar water disinfection reactors using the E-screen bioassay and the Ames test

2021 ◽  
Author(s):  
Paloma Ozores Diez ◽  
M. Inmaculada Polo-López ◽  
Azahara Martínez-García ◽  
Monique Waso ◽  
Brandon Reyneke ◽  
...  
Keyword(s):  
Ames Test ◽  
Daily Intake ◽  
Cost Effective ◽  
Water Disinfection ◽  
In Vitro Toxicity ◽  
Toxic Substances ◽  
Solar Water ◽  
Point Of Use ◽  
Solar Water Disinfection

Abstract Solar water disinfection (SODIS) is a cost-effective point of use method for disinfecting water, usually in a 2 L polyethylene terephthalate (PET) plastic bottle. To increase the volume of water disinfected, three novel transparent reactors were developed using PET in 25 L transparent jerrycans, polymethyl methacrylate (PMMA) in tubular solar reactors capable of delivering >20 L of water and polypropylene (PP) in 20 L buckets. In vitro bioassays were used to investigate any toxic substances leached from the plastic reactors into disinfected water as a result of exposure to sunshine for up to 9 months. The Ames test was used to test for mutagenicity and the E-screen bioassay to test for estrogenicity. No mutagenicity was detected in any sample and no estrogenicity was found in the SODIS treated water produced by the PMMA reactors or the PP buckets. While water disinfected using the PET reactors showed no estrogenicity following exposure to the sun for 3 and 6 months, estrogenicity was detected following 9 months' exposure to sunlight; however levels detected were within the acceptable daily intake for 17β-estradiol (E2) of up to 50 ng/kg body weight/day.

scholarly journals Wastewaters of meat-processing enterprise: assessment of genotoxic potential

2018 ◽  
Vol 245 ◽  
pp. 18002 ◽  
Author(s):  
Olga Ivanchenko ◽  
Rustem Khabibullin ◽  
Rahat Bhat
Keyword(s):  
Waste Water ◽  
Ames Test ◽  
Excision Repair ◽  
Mutagenic Activity ◽  
Liver Microsomes ◽  
Metabolic Activation ◽  
Rat Liver Microsomes ◽  
Meat Processing ◽  
Processing Enterprise

Environmental pollution and ecosystem dysfunction are one of the most important problems of the today’s world. Assessment of toxigenic properties of effluents from the meat-processing enterprise was carried out using the short-term microorganisms biotests in vitro. Both native waste water and its ether and water fractions were investigated. The probes’ sterilization was carried out by filtration through the sterile membrane filters Synpor with pores diameter of 0.45 m. Mutagenic activity of wastewaters was determined using the Salmonella/microsomes plate with in vitro metabolic activation and without metabolic activation (Ames test). As a metobolic activation the rat liver microsomes were used. Studying of the DNA-damaging activity was carried out using the suspension method modification on the mutant Escherichia coli strains, in which the functioning of one reparation systems is suppressed: uvrA-, recAand рol A-. Native waste water doesn’t have an influence on the mutant strains recAand рol A-, its survivability degree is in the range 96-100%. However, DNA-damaging action was registered for the strain with the damaged excision repair (uvrA-), survivability of which was 81.31%. Ames test of wastewater and its fractions didn’t reveal any mutagenic activity. The tests used in this work allow one to comprehensively estimate the genetic danger of the enterprise wastewaters within a short time and are recommended as test-systems for monitoring the ecological safety of wastewaters.

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