scholarly journals Serologic test systems development. Progress report, October 1, 1977-September 30, 1978. [Applications of enzyme immunoassay in agriculture]

1979 ◽  
Author(s):  
G Seawright ◽  
M Bartlett ◽  
W Sanders ◽  
E Clinard ◽  
K Mills ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Systems Development ◽  
Progress Report ◽  
Serologic Test ◽  
Test Systems

scholarly journals Serologic test systems development. Progress report, October 1, 1978-September 30, 1979

1980 ◽  
Author(s):  
G Seawright ◽  
W Sanders ◽  
U Hollstein ◽  
J Butler ◽  
K Mills ◽  
...  
Keyword(s):  
Systems Development ◽  
Progress Report ◽  
Serologic Test ◽  
Test Systems

scholarly journals Serologic test systems development. Progress report, July 1, 1976--September 30, 1977

1978 ◽  
Author(s):  
G Saunders ◽  
E Clinard ◽  
M Bartlett ◽  
P Petersen ◽  
W Sanders ◽  
...  
Keyword(s):  
Systems Development ◽  
Progress Report ◽  
Serologic Test ◽  
Test Systems

scholarly journals Serologic test systems development. Semiannual report, July--December 1973

1974 ◽  
Author(s):  
G Saunders ◽  
E Clinard ◽  
J Forslund ◽  
M Bartlett ◽  
D Holm
Keyword(s):  
Systems Development ◽  
Serologic Test ◽  
Test Systems

scholarly journals PROFILES OF CYTOKINES IN MICE DURING IMMUNIZATION WITH ADTP-VACCINE WITH ACELLULAR PERTUSSIS COMPONENT

2016 ◽  
pp. 49-53
Author(s):  
E. M. Zaitsev ◽  
A. V. Poddubikov ◽  
M. V. Britsina ◽  
M. N. Ozeretskovskaya ◽  
N. U. Mertsalova ◽  
...  
Keyword(s):  
Immune Response ◽  
Enzyme Immunoassay ◽  
Pertussis Vaccine ◽  
Control Group ◽  
Pertussis Infection ◽  
Commercial Test ◽  
Test Systems ◽  
Protective Antigens ◽  
Tetanus Vaccine ◽  
Stimulation Of

Aim. Study cytokine status in mice immunized with vaccines containing acellular pertussis component. Materials and methods. Vaccines developed in Mechnikov RIVS - acellular pertussis vaccine (aPV) and adsorbed pertussis-diphtheria-tetanus vaccine (aDTaP), containing a complex of protective antigens of pertussis microbe - were used in the study. Fi (CBAxCsyBle) line mice weighing 12 - 14 g were immunized intraperitoneally 3 times at an interval of 7 days with aPV and aDTaP at human immunization dose (0.5 ml), containing 25 pg of pertussis component. Intact mice were used as a control group. Levels of IFN-y, IL-2, IL-4, IL-5, IL-12 cytokines were determined after each immunization in enzyme immunoassay using commercial test-systems from Cusabio (China). Results. An increase of levels of IFN-y, IL-2, IL-5, IL-12 and lack of stimulation of production of IL-4 was established in dynamics of immune response after administration of aPV and aDTaP vaccines. Conclusion. The data obtained indicate that immunization of mice with aPV and aDTaP vaccines resulted in activation of production of cytokines characteristic for immune response during pertussis infection and immunization with whole-cellular aDTP-vaccines.

The combined use of synthetic and biological test-systems in the development of transdermal therapeutic systems

2020 ◽  
pp. 49-58
Author(s):  
E. G. Kuznetsova ◽  
◽  
O. M. Kuryleva ◽  
L. A. Salomatina ◽  
V. I. Sevastianov ◽  
...  
Keyword(s):  
Systems Development ◽  
Test System ◽  
Preclinical Studies ◽  
Biological Test ◽  
Diffusion Cell ◽  
Rabbit Skin ◽  
Franz Diffusion Cell ◽  
Test Systems ◽  
Combined Use ◽  
Synthetic Test

There are several approaches to modeling percutaneous diffusion of drugs and predicting its effectiveness in the development of new transdermal therapeutic systems (TTS): mathematical models, synthetic and biological test-systems. The aim of the work is to substantiate the need to combined use synthetic and biological test systems at the initial stages of the transdermal therapeutic systems development using the Galavit® immunomodulator TTS as an example. Six batches of laboratory samples of TTS were made with a different composition of excipients in emulsions. Transdermal therapeutic systems formulations were screened on the synthetic test-system represented by Strat-M membrane (Merck Millipore). Based on the results of the study, two formulations with the best data of the drugs amount passed into the receiving chamber of the Franz diffusion cell were selected. Amount of drugs in the form were amounted 30 %. The use of non-preserved rabbit skin was revealed significant differences in this indicator for TTS of these two compositions. The mass of drugs passing through the skin from the TTS Galavit® for 24 hours of application for the first emulsion was 58 – 71 % and was 39 – 50 % for the second. The biological test-system turned out to be more sensitive to the emulsion compositions. Thus, the combined use of synthetic and biological test-systems makes it possible to significantly reduce the complexity and costs of preclinical studies of new TTS.

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