Statement of work for the immobilized low-activity waste transportation system -- Project W-465

SummaryThe thrombogenic effects of selected factor IX concentrates were evaluated in two rabbit models; the Wessler stasis model and a novel non-stasis model. Concentrates active in either the NAPTT or TGt50 in vitro tests of potential thrombogenicity, or both, caused thrombus formation in the Wessler technique and activation of the coagulation system in the non-stasis model. A concentrate with low activity in both in vitro tests did not have thrombogenic effects in vivo, at the chosen dose. Results in the non-stasis model suggested that the thrombogenic effects of factor IX concentrates may occur by at least two mechanisms. A concentrate prepared from platelet-rich plasma and a pyrogenic concentrate were also tested and found to have no thrombogenic effect in vivo.These studies justify the use of the NAPTT and TGt50 in vitro tests for the screening of factor IX concentrates prior to clinical use.

Download Full-text

Separation of Heparin into Subtractions by DEAE-Cellulose Chromatography

Thrombosis and Haemostasis ◽

10.1055/s-0038-1657046 ◽

1979 ◽

Vol 42 (05) ◽

pp. 1452-1459 ◽

Cited By ~ 4

Author(s):

Robert H Yue ◽

Toby Starr ◽

Menard M Gertler

Keyword(s):

High Activity ◽

Uronic Acid ◽

Deae Cellulose ◽

Exchange Chromatography ◽

Net Charge ◽

Uronic Acid Content ◽

Successful Separation ◽

Salt Gradient ◽

Structure And Activity ◽

Low Activity

SummaryCommercial porcine heparin can be separated into three distinct subtractions by using DEAE-cellulose chromatography and a stepped salt gradient. Gram quantities of heparin can be fractionated by this technique. All three heparin subtractions can accelerate the inhibition of thrombin by antithrombin III with different efficiency. The specific activities of the high activity heparin, intermediate activity heparin and low activity heparin are 228 units/mg, 142 units/mg and 95 units/mg, respectively. Both the uronic acid content and the quantity of N-SO4 for all three heparin subfractions have been evaluated. The high activity heparin has the lowest uronic acid and N-SO4 content. The successful separation of commercial heparin into three distinct subfractions by means of ion-exchange chromatography suggests that the net charge on these three heparin components will serve as a model system in the elucidation of the structure and activity relationship to the biological function of heparin.

Download Full-text