scholarly journals Instrumentation for high-efficiency, high-sensitivity actinide analysis

1996 ◽  
Author(s):  
J. Olivares ◽  
E. Chamberlin ◽  
M. Murrell ◽  
M. Kahr ◽  
Y. Duan
Keyword(s):  
High Efficiency ◽  
High Sensitivity ◽  
Actinide Analysis

Trends in Diagnosis for Active Tuberculosis Using Nanomaterials

2019 ◽  
Vol 26 (11) ◽  
pp. 1946-1959 ◽  
Author(s):  
Le Minh Tu Phan ◽  
Lemma Teshome Tufa ◽  
Hwa-Jung Kim ◽  
Jaebeom Lee ◽  
Tae Jung Park
Keyword(s):  
Sensitivity And Specificity ◽  
High Efficiency ◽  
Point Of Care ◽  
High Sensitivity ◽  
Signs And Symptoms ◽  
Diagnostic Methods ◽  
Advantages And Disadvantages ◽  
Treatment And Prevention ◽  
Clinical Tools ◽  
Diagnostic Applications

Background:Tuberculosis (TB), one of the leading causes of death worldwide, is difficult to diagnose based only on signs and symptoms. Methods for TB detection are continuously being researched to design novel effective clinical tools for the diagnosis of TB.Objective:This article reviews the methods to diagnose TB at the latent and active stages and to recognize prospective TB diagnostic methods based on nanomaterials.Methods:The current methods for TB diagnosis were reviewed by evaluating their advantages and disadvantages. Furthermore, the trends in TB detection using nanomaterials were discussed regarding their performance capacity for clinical diagnostic applications.Results:Current methods such as microscopy, culture, and tuberculin skin test are still being employed to diagnose TB, however, a highly sensitive point of care tool without false results is still needed. The utilization of nanomaterials to detect the specific TB biomarkers with high sensitivity and specificity can provide a possible strategy to rapidly diagnose TB. Although it is challenging for nanodiagnostic platforms to be assessed in clinical trials, active TB diagnosis using nanomaterials is highly expected to achieve clinical significance for regular application. In addition, aspects and future directions in developing the high-efficiency tools to diagnose active TB using advanced nanomaterials are expounded.Conclusion:This review suggests that nanomaterials have high potential as rapid, costeffective tools to enhance the diagnostic sensitivity and specificity for the accurate diagnosis, treatment, and prevention of TB. Hence, portable nanobiosensors can be alternative effective tests to be exploited globally after clinical trial execution.

scholarly journals Search for Promising Strains of Probiotic Microbiota Isolated from Different Biotopes of Healthy Cats for Use in the Control of Surgical Infections

Pathogens ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 667
Author(s):  
Pavel Rudenko ◽  
Yuriy Vatnikov ◽  
Nadezhda Sachivkina ◽  
Andrei Rudenko ◽  
Evgeny Kulikov ◽  
...  
Keyword(s):  
Antimicrobial Agents ◽  
High Efficiency ◽  
Biological Marker ◽  
High Sensitivity ◽  
Antagonistic Activity ◽  
Adhesive Properties ◽  
Surgical Infection ◽  
Surgical Infections ◽  
Causative Agents ◽  
Inflammatory Processes

Despite the introduction of modern methods of treatment, the creation of new generations of antibacterial agents, and the constant improvement of aseptic and antiseptic methods, the treatment of purulent–inflammatory processes remains one of the most complex and urgent problems in veterinary practice. The article presents the results of the isolation of indigenous microbiota from various biotopes of healthy cats, as well as the study of their biological marker properties for the selection of the most optimal strains in probiotic medicines for the control of surgical infections. It was demonstrated that isolated cultures of bifidobacteria and lactobacilli, which we isolated, revealed high sensitivity to antibiotics of the β-lactam group (excepting L. acidophilus No. 24, L. plantarum “Victoria” No. 22, L. rhamnosus No. 5, L. rhamnosus No. 20, and L. rhamnosus No. 26, which showed a significant variability in sensitivity to antibacterial drugs of this group, indicating the great potential of these microorganisms) and resistance to aminoglycosides, lincosamides, and fluoroquinolones (with the exception of gatifloxacin, which showed high efficiency in relation to all lactic acid microorganisms). The adhesive properties of the isolated lactobacteria and bifidobacteria were variable, even within the same species. It was found that the B. adolescentis No. 23 strain of the Bifidobacterium genus, as well as the L. plantarum No. 8, L. plantarum “Victoria” No. 22, L. rhamnosus No. 6, L. rhamnosus No. 26, L. acidophilus No. 12, and L. acidophilus No. 24 strains of the Lactobacillus genus had the highest adhesive activity. Thus, when conducting a detailed analysis of the biological marker properties of candidate cultures (determining their sensitivity to antimicrobial agents, studying the adhesive properties, and antagonistic activity in relation to causative agents of surgical infection in cats), it was found that the most promising are L. plantarum “Victoria” No. 22, L. rhamnosus No. 26, and L. acidophilus No. 24.

Low-frequency multiplex CARS microscopy with a high-repetition near-infrared supercontinuum laser

2021 ◽  
Author(s):  
Yusuke Arashida ◽  
Atsushi Taninaka ◽  
Takayuki Ochiai ◽  
Hiroyuki Mogi ◽  
Shoji YOSHIDA ◽  
...  
Keyword(s):  
Near Infrared ◽  
High Efficiency ◽  
Signal To Noise Ratio ◽  
Low Frequency ◽  
High Sensitivity ◽  
High Repetition ◽  
Spectrum Shaping ◽  
Crystal Phase Transition ◽  
Wavenumber Region ◽  
Anti Stokes

Abstract We have developed a multiplex Coherent anti-Stokes Raman scattering (CARS) microscope effective for low-wavenumber measurement by combining a high-repetition supercontinuum light source of 1064 nm and an infrared high-sensitivity InGaAs diode array. This system could observe the low-wavenumber region down to 55 cm-1 with high sensitivity. In addition, using spectrum shaping and spectrum modulation techniques, we simultaneously realized a wide bandwidth (<1800 cm-1), high wavenumber resolution (9 cm-1), high efficiency, and increasing signal to noise ratio by reducing the effect of the background shape in low-wavenumber region. Spatial variation of a sulfur crystal phase transition with metastable states was visualized.

Aerothermal Simulations Comparisons of a Shaped-Hole Film Cooling Flow

2019 ◽  
Author(s):  
Matthieu Simon ◽  
Sébastien Gautier ◽  
Emmanuel Vanoli ◽  
Pierre Auzillon
Keyword(s):  
Numerical Simulations ◽  
Film Cooling ◽  
Test Bench ◽  
High Efficiency ◽  
Density Ratio ◽  
High Sensitivity ◽  
Good Prediction ◽  
Ansys Fluent ◽  
Free Stream Turbulence ◽  
Complete Test

Abstract Film Cooling is a crucial technology for engine manufacturer to develop high-efficiency gas turbine engines by raising turbine entry temperature. A lot of cooling holes geometries have been studied in the past few years in tests, as well as numerical simulations. Shaped holes are nowadays a standard geometry for protecting the blades, given the performance improvement compared to cylindrical holes. Numerical correlation with physical tests is challenging due to the high sensitivity to thermal mixing and adequate boundary condition predictions. This paper is devoted to numerical simulation comparisons of the 777 shaped holes configuration of Pennsylvania State University, for an incompressible flow with a density ratio of 1.5, a blowing ratio of 1.5 and a free stream turbulence intensity of 0.5%. Two different simulations have been chosen: a state-of-the-art RANS simulation with k-e Realizable model computed with ANSYS Fluent and a high fidelity solver Lattice-Boltzmann Method computed with Simulia PowerFLOW. In order to improve the accuracy of numerical simulations against test results, this article deals with an aerothermal model of the complete test bench. This additional modeling allows to strongly improve thermal prediction and to understand initial discrepancies related to test bench environment. Results show that k-ε Realizable simulation provides a good prediction of average effectiveness, but local differences appear due to inherent RANS modeling limitations. On the other hand, LBM simulation provides excellent results for both aerodynamic and thermal quantities: tests results are very well reproduced.

scholarly journals Steady-State Kinetics of Enzyme-Catalyzed Hydrolysis of Echothiophate, a P–S Bonded Organophosphorus as Monitored by Spectrofluorimetry

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1371 ◽  
Author(s):  
Irina V. Zueva ◽  
Sofya V. Lushchekina ◽  
David Daudé ◽  
Eric Chabrière ◽  
Patrick Masson
Keyword(s):  
Steady State ◽  
High Efficiency ◽  
High Sensitivity ◽  
High Rate ◽  
Paraoxonase 1 ◽  
Brevundimonas Diminuta ◽  
Steady State Kinetics ◽  
Steady State Kinetic ◽  
Thiol Reagent ◽  
Hydrolysis Of

Enzyme-catalyzed hydrolysis of echothiophate, a P–S bonded organophosphorus (OP) model, was spectrofluorimetrically monitored, using Calbiochem Probe IV as the thiol reagent. OP hydrolases were: the G117H mutant of human butyrylcholinesterase capable of hydrolyzing OPs, and a multiple mutant of Brevundimonas diminuta phosphotriesterase, GG1, designed to hydrolyze a large spectrum of OPs at high rate, including V agents. Molecular modeling of interaction between Probe IV and OP hydrolases (G117H butyrylcholinesterase, GG1, wild types of Brevundimonas diminuta and Sulfolobus solfataricus phosphotriesterases, and human paraoxonase-1) was performed. The high sensitivity of the method allowed steady-state kinetic analysis of echothiophate hydrolysis by highly purified G117H butyrylcholinesterase concentration as low as 0.85 nM. Hydrolysis was michaelian with Km = 0.20 ± 0.03 mM and kcat = 5.4 ± 1.6 min−1. The GG1 phosphotriesterase hydrolyzed echothiophate with a high efficiency (Km = 2.6 ± 0.2 mM; kcat = 53400 min−1). With a kcat/Km = (2.6 ± 1.6) × 107 M−1min−1, GG1 fulfills the required condition of potential catalytic bioscavengers. quantum mechanics/molecular mechanics (QM/MM) and molecular docking indicate that Probe IV does not interact significantly with the selected phosphotriesterases. Moreover, results on G117H mutant show that Probe IV does not inhibit butyrylcholinesterase. Therefore, Probe IV can be recommended for monitoring hydrolysis of P–S bonded OPs by thiol-free OP hydrolases.

scholarly journals Marine Toxins Detection by Biosensors Based on Aptamers

Toxins ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 1 ◽  
Author(s):  
Wei Ye ◽  
Taomei Liu ◽  
Weimin Zhang ◽  
Muzi Zhu ◽  
Zhaoming Liu ◽  
...  
Keyword(s):  
Detection Limit ◽  
Sensitivity And Specificity ◽  
High Efficiency ◽  
Rapid Development ◽  
High Sensitivity ◽  
Detection Methods ◽  
Marine Toxins ◽  
Time Saving ◽  
Low Detection Limit ◽  
The Future

Marine toxins cause great harm to human health through seafood, therefore, it is urgent to exploit new marine toxins detection methods with the merits of high sensitivity and specificity, low detection limit, convenience, and high efficiency. Aptasensors have emerged to replace classical detection methods for marine toxins detection. The rapid development of molecular biological approaches, sequencing technology, material science, electronics and chemical science boost the preparation and application of aptasensors. Taken together, the aptamer-based biosensors would be the best candidate for detection of the marine toxins with the merits of high sensitivity and specificity, convenience, time-saving, relatively low cost, extremely low detection limit, and high throughput, which have reduced the detection limit of marine toxins from nM to fM. This article reviews the detection of marine toxins by aptamer-based biosensors, as well as the selection approach for the systematic evolution of ligands by exponential enrichment (SELEX), the aptamer sequences. Moreover, the newest aptasensors and the future prospective are also discussed, which would provide thereotical basis for the future development of marine toxins detection by aptasensors.

Metal Magnetic Memory Testing Technique for Typical Defects in Pressure Vessels and Pipelines

2015 ◽  
Author(s):  
Weihe Guan ◽  
Pengju Guo ◽  
Chen Xuedong
Keyword(s):  
High Efficiency ◽  
Low Cost ◽  
Orientation Angle ◽  
High Sensitivity ◽  
Pressure Vessels ◽  
Magnetic Memory ◽  
Metal Magnetic Memory ◽  
Time Saving ◽  
Hydrogen Induced Cracking ◽  
Memory Signals

Metal magnetic memory technique has been extensively applied in different fields due to its unique advantages of time-saving, low cost, and high efficiency. However, very limited research has been carried out on studying the characteristics of metal magnetic memory signals of different defects except crack, and also the effect of orientation angle between testing direction and defect on magnetic memory signals. To promote study in this area, the magnetic memory signals of typical defects (such as crack, slag inclusion) are investigated as well as hydrogen-induced cracking. In addition, the characteristics of magnetic memory signals when measured with different angle between testing direction and defects were obtained. The results indicate that the metal magnetic memory technique is a promising method to detect typical defects of welding and also hydrogen-induced cracking. Moreover, the technique has high sensitivity on defects no matter the angle between testing direction and defect. However, further research is needed because it can only find the possible location of defects but cannot quantitatively describe the defect.

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