scholarly journals In vitro evolution-enabled selection of affinity reagents against bio/chem-threats

2021 ◽  
Author(s):  
Antonietta Lillo
Keyword(s):  
In Vitro Evolution ◽  
Affinity Reagents ◽  
Selection Of

scholarly journals Selection of a T7 promoter mutant with enhanced in vitro activity by a novel multi-copy bead display approach for in vitro evolution

2012 ◽  
Vol 41 (1) ◽  
pp. e29-e29 ◽  
Author(s):  
Siddhartha Paul ◽  
Alexander Stang ◽  
Klaus Lennartz ◽  
Matthias Tenbusch ◽  
Klaus Überla
Keyword(s):  
Vitro Activity ◽  
In Vitro Activity ◽  
T7 Promoter ◽  
In Vitro Evolution ◽  
Selection Of ◽  
Promoter Mutant

ChemInform Abstract: In Vitro Evolution and Selection of Proteins: Ribosome Display for Larger Libraries

ChemInform ◽  
2010 ◽  
Vol 30 (17) ◽  
pp. no-no
Author(s):  
Ulrich Hoffmueller ◽  
Jens Schneider-Mergener
Keyword(s):  
In Vitro Evolution ◽  
Ribosome Display ◽  
Selection Of

scholarly journals Influence of Target Concentration and Background Binding on In Vitro Selection of Affinity Reagents

PLoS ONE ◽  
2012 ◽  
Vol 7 (8) ◽  
pp. e43940 ◽  
Author(s):  
Jinpeng Wang ◽  
Joseph F. Rudzinski ◽  
Qiang Gong ◽  
H. Tom Soh ◽  
Paul J. Atzberger
Keyword(s):  
In Vitro Selection ◽  
Target Concentration ◽  
Affinity Reagents ◽  
Selection Of

scholarly journals Protein Structural Information and Evolutionary Landscape by In Vitro Evolution

2019 ◽  
Vol 37 (4) ◽  
pp. 1179-1192 ◽  
Author(s):  
Marco Fantini ◽  
Simonetta Lisi ◽  
Paolo De Los Rios ◽  
Antonino Cattaneo ◽  
Annalisa Pastore
Keyword(s):  
Protein Structure ◽  
Structural Information ◽  
Phenotypic Selection ◽  
Natural Evolution ◽  
In Vitro Evolution ◽  
Physical Measurements ◽  
Evolution Understanding ◽  
Protein Variants ◽  
Selection Of

Abstract Protein structure is tightly intertwined with function according to the laws of evolution. Understanding how structure determines function has been the aim of structural biology for decades. Here, we have wondered instead whether it is possible to exploit the function for which a protein was evolutionary selected to gain information on protein structure and on the landscape explored during the early stages of molecular and natural evolution. To answer to this question, we developed a new methodology, which we named CAMELS (Coupling Analysis by Molecular Evolution Library Sequencing), that is able to obtain the in vitro evolution of a protein from an artificial selection based on function. We were able to observe with CAMELS many features of the TEM-1 beta-lactamase local fold exclusively by generating and sequencing large libraries of mutational variants. We demonstrated that we can, whenever a functional phenotypic selection of a protein is available, sketch the structural and evolutionary landscape of a protein without utilizing purified proteins, collecting physical measurements, or relying on the pool of natural protein variants.

Clone selection of in vitro horseradish hairy root cultures

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
R Bertóti ◽  
Á Alberti ◽  
A Böszörményi ◽  
R Könye ◽  
T Horváth ◽  
...  
Keyword(s):  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Clone Selection ◽  
Selection Of
Sign in / Sign up

Export Citation Format

Share Document