scholarly journals Preliminary Study of Potential Utilization of Triassic Rift Basins for Long-Term Carbon Sequestration

2020 ◽  
Author(s):  
David Brezinski ◽  
◽  
Rebecca Kavage Adams
Keyword(s):  
Carbon Sequestration ◽  
Rift Basins ◽  
Preliminary Study

Ultrastructural observations of the aorta of calves implanted with a mechanical left ventricular assist device

1990 ◽  
Vol 48 (3) ◽  
pp. 834-835
Author(s):  
J P Cassella ◽  
V Salih ◽  
T R Graham
Keyword(s):  
Left Ventricular Assist Device ◽  
Ventricular Assist Device ◽  
Left Ventricular ◽  
Aortic Tissue ◽  
Assist Device ◽  
Ventricular Assist ◽  
Transmission Electron ◽  
Left Ventricular Assist ◽  
Preliminary Study

Left ventricular assist systems are being developed for eventual long term or permanent implantation as an alternative to heart transplantation in patients unsuitable for or denied the transplant option. Evaluation of the effects of these devices upon normal physiology is required. A preliminary study was conducted to evaluate the morphology of aortic tissue from calves implanted with a pneumatic Left Ventricular Assist device-LVAD. Two 3 month old heifer calves (calf 1 and calf 2) were electively explanted after 128 days and 47 days respectively. Descending thoracic aortic tissue from both animals was removed immediately post mortem and placed into karnovsky’s fixative. The tissue was subsequently processed for transmission electron microscopy (TEM). Some aortic tissue was fixed in neutral buffered formalin and processed for routine light microscopy.

scholarly journals Partial Decellularization for Segmental Tracheal Scaffold Tissue Engineering: A Preliminary Study in Rabbits

Biomolecules ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 866
Author(s):  
Luong Huu Dang ◽  
Yuan Tseng ◽  
How Tseng ◽  
Shih-Han Hung
Keyword(s):  
Mechanical Stability ◽  
Vital Staining ◽  
Potential Method ◽  
Term Survival ◽  
Long Term Survival ◽  
Cartilage Cells ◽  
Preliminary Study ◽  
Epithelial Layers

In this study, we developed a new procedure for the rapid partial decellularization of the harvested trachea. Partial decellularization was performed using a combination of detergent and sonication to completely remove the epithelial layers outside of the cartilage ring. The post-decellularized tracheal segments were assessed with vital staining, which showed that the core cartilage cells remarkably remained intact while the cells outside of the cartilage were no longer viable. The ability of the decellularized tracheal segments to evade immune rejection was evaluated through heterotopic implantation of the segments into the chest muscle of rabbits without any immunosuppressive therapy, which demonstrated no evidence of severe rejection or tissue necrosis under H&E staining, as well as the mechanical stability under stress-pressure testing. Finally, orthotopic transplantation of partially decellularized trachea with no immunosuppression treatment resulted in 2 months of survival in two rabbits and one long-term survival (2 years) in one rabbit. Through evaluations of posttransplantation histology and endoscopy, we confirmed that our partial decellularization method could be a potential method of producing low-immunogenic cartilage scaffolds with viable, functional core cartilage cells that can achieve long-term survival after in vivo transplantation.

The impact of porosity on organic matter cycling in a two-dimensional porous medium 

2021 ◽  
Author(s):  
Hanbang Zou ◽  
Pelle Ohlsson ◽  
Edith Hammer
Keyword(s):  
Porous Medium ◽  
Organic Matter ◽  
Carbon Sequestration ◽  
Soil Carbon ◽  
Pore Network ◽  
Pore Scale ◽  
Decomposition Of Organic Matter ◽  
Organic Matter Cycling ◽  
The Impact

<p>Carbon sequestration has been a popular research topic in recent years as the rapid elevation of carbon emission has significantly impacted our climate. Apart from carbon capture and storage in e.g. oil reservoirs, soil carbon sequestration offers a long term and safe solution for the environment and human beings. The net soil carbon budget is determined by the balance between terrestrial ecosystem sink and sources of respiration to atmospheric carbon dioxide. Carbon can be long term stored as organic matters in the soil whereas it can be released from the decomposition of organic matter. The complex pore networks in the soil are believed to be able to "protect" microbial-derived organic matter from decomposition. Therefore, it is important to understand how soil structure impacts organic matter cycling at the pore scale. However, there are limited experimental studies on understanding the mechanism of physical stabilization of organic matter. Hence, my project plan is to create a heterogeneous microfluidic porous microenvironment to mimic the complex soil pore network which allows us to investigate the ability of organisms to access spaces starting from an initial ecophysiological precondition to changes of spatial accessibility mediated by interactions with the microbial community.</p><p>Microfluidics is a powerful tool that enables studies of fundamental physics, rapid measurements and real-time visualisation in a complex spatial microstructure that can be designed and controlled. Many complex processes can now be visualized enabled by the development of microfluidics and photolithography, such as microbial dynamics in pore-scale soil systems and pore network modification mimicking different soil environments – earlier considered impossible to achieve experimentally. The microfluidic channel used in this project contains a random distribution of cylindrical pillars of different sizes so as to mimic the variations found in real soil. The randomness in the design creates various spatial availability for microbes (preferential flow paths with dead-end or continuous flow) as an invasion of liquids proceeds into the pore with the lowest capillary entry pressure. In order to study the impact of different porosity in isolation of varying heterogeneity of the porous medium, different pore size chips that use the same randomly generated pore network is created. Those chips have the same location of the pillars, but the relative size of each pillar is scaled. The experiments will be carried out using sterile cultures of fluorescent bacteria, fungi and protists, synthetic communities of combinations of these, or a whole soil community inoculum. We will quantify the consumption of organic matter from the different areas via fluorescent substrates, and the bio-/necromass produced. We hypothesise that lower porosity will reduce the net decomposition of organic matter as the narrower pore throat limits the access, and that net decomposition rate at the main preferential path will be higher than inside branches</p>

Green manure: Alternative to carbon sequestration in a Typic Ustipsamment under semiarid conditions

2018 ◽  
Vol 8 ◽  
Author(s):  
Nelson Virgilio Piraneque Gambasica ◽  
Sonia Esperanza Aguirre Forero ◽  
Adriano Reis Lucheta
Keyword(s):  
Carbon Sequestration ◽  
Cover Crops ◽  
Green Manure ◽  
Block Design ◽  
Climatic Variability ◽  
Plant Cover ◽  
Caribbean Region ◽  
Full Potential ◽  
Semiarid Conditions

Vegetative soil cover mitigates climatic variability and enhances the balance between mineralization and humification processes. Under aerobic conditions, most of the carbon that enters the soil is labile, but a small fraction (1%) is humified and stable, contributing to the soil carbon reserve; therefore, it is important to assess the carbon content captured after green manure cultivation and decomposition. During two consecutive semesters, July to December 2016 and January to June 2017, green manure plots (<em>Zea mays </em>L., <em>Andropogon sorghum </em>subsp.<em> sudanensis </em>and <em>Crotalaria longirostrata</em>) were cultivated individually, in a consortium or amended with palm oil agro-industrial biosolids in a randomized complete block design with 12 treatments. Once decomposed, the different carbon fractions (organic, oxidizable, non-oxidizable, removable and total) were determined. The results showed high total and organic carbon contents under the sorghum treatment, at 30 and 28 Mg ha<sup>-1</sup>, respectively, followed by those under the fallow + biosolid treatment, at 29.8 Mg ha<sup>-1</sup> and 27.5 Mg ha<sup>-1</sup>, respectively. Despite the short experiment duration and the possible contributions of previous management on recalcitrant carbon soil stocks, these findings suggest the importance of maintaining plant cover and utilizing green manure in the Colombian Caribbean region. Long-term experiments may be conducted to confirm the full potential of cover crops on carbon sequestration under tropical semiarid conditions.

scholarly journals Additional Benefits of Community Managed Forest: A Case Study of Champadevi Community Forest

2012 ◽  
Vol 12 ◽  
pp. 127-132
Author(s):  
Bhanu B Panthi
Keyword(s):  
Carbon Sequestration ◽  
Significant Contribution ◽  
Research Area ◽  
Environmental Benefits ◽  
Managed Forests ◽  
Managed Forest ◽  
Community Based

This research attempts to identify the existing condition of the community managed forest based on the assumption that it will serve as a proxy for the condition of other forests in the mid hills region of Nepal. The research area has an atypical variation in altitude and diverse pattern of vegetation. This study mainly focuses on estimating carbon content in the forest and identifying the species that has more carbon storage capacity. The research signifies the role of forests in mitigation of ‘Global warming’ and ‘Climate change’ by storing carbon in tree biomass. These types of community based forest management programs are significant for their additional carbon sequestration through the avoidance of deforestation and degradation. The carbon sequestration have a significant contribution to environmental benefits, any shrinkage of forests have an enormous impact on CO2 emission with long term consequences. Thus, the development and expansion of community managed forests provide many benefits to the adjacent community and globally at large.DOI: http://dx.doi.org/10.3126/njst.v12i0.6490 Nepal Journal of Science and Technology 12 (2011) 127-32 

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