scholarly journals Role of the Rubisco Small Subunit

2016 ◽  
Author(s):  
Robert Joseph Spreitzer
Keyword(s):  
Small Subunit ◽  
Rubisco Small Subunit

Modulation of procaspase-7 self-activation by PEST amino acid residues of the N-terminal prodomain and intersubunit linker

2017 ◽  
Vol 95 (6) ◽  
pp. 634-643
Author(s):  
Juliano Alves ◽  
Miguel Garay-Malpartida ◽  
João M. Occhiucci ◽  
José E. Belizário
Keyword(s):  
Amino Acid ◽  
Large Subunit ◽  
Small Subunit ◽  
Cleavage Sites ◽  
Amino Acid Residues ◽  
Wild Type ◽  
Caspase 7 ◽  
Caspase Family ◽  
The Impact

Procaspase-7 zymogen polypeptide is composed of a short prodomain, a large subunit (p20), and a small subunit (p10) connected to an intersubunit linker. Caspase-7 is activated by an initiator caspase-8 and -9, or by autocatalysis after specific cleavage at IQAD198↓S located at the intersubunit linker. Previously, we identified that PEST regions made of amino acid residues Pro (P), Glu (E), Asp (D), Ser (S), Thr (T), Asn (N), and Gln (Q) are conserved flanking amino acid residues in the cleavage sites within a prodomain and intersubunit linker of all caspase family members. Here we tested the impact of alanine substitution of PEST amino acid residues on procaspase-7 proteolytic self-activation directly in Escherichia coli. The p20 and p10 subunit cleavage were significantly delayed in double caspase-7 mutants in the prodomain (N18A/P26A) and intersubunit linker (S199A/P201A), compared with the wild-type caspase-7. The S199A/P201A mutants effectively inhibited the p10 small subunit cleavage. However, the mutations did not change the kinetic parameters (kcat/KM) and optimal tetrapeptide specificity (DEVD) of the purified mutant enzymes. The results suggest a role of PEST-amino acid residues in the molecular mechanism for prodomain and intersubunit cleavage and caspase-7 self-activation.

scholarly journals Isolation and characterization of rubisco small subunit gene promoter from common wheat (Triticum aestivumL.)

2015 ◽  
Vol 10 (2) ◽  
pp. e989033 ◽  
Author(s):  
Shalini Mukherjee ◽  
Claudio Stasolla ◽  
Anita Brûlé-Babel ◽  
Belay T Ayele
Keyword(s):  
Common Wheat ◽  
Gene Promoter ◽  
Small Subunit ◽  
Subunit Gene ◽  
Isolation And Characterization ◽  
Rubisco Small Subunit

scholarly journals Identification of Encephalitozoon intestinalis in Travelers with Chronic Diarrhea by Specific PCR Amplification

1998 ◽  
Vol 36 (1) ◽  
pp. 37-40 ◽  
Author(s):  
Laurent Raynaud ◽  
Frederic Delbac ◽  
Veronique Broussolle ◽  
Meja Rabodonirina ◽  
Veronique Girault ◽  
...  
Keyword(s):  
Chronic Diarrhea ◽  
Pcr Amplification ◽  
Small Subunit ◽  
Small Subunit Rrna ◽  
Specific Pcr ◽  
Encephalitozoon Intestinalis ◽  
Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Screening ◽  
Virus Screening

With the use of Weber’s modified trichrome and Uvitex 2B techniques, spores of microsporidia were detected in the stools of four travelers presenting clinically with chronic diarrhea. The general health of these patients was not impaired, and human immunodeficiency virus screening was negative. Immune evaluation, including the study of lymphocytic subpopulations, assay of serum immunoglobulins, and an intradermal multitest, showed normal results. Molecular identification of microsporidian species was based on the PCR amplification of a small-subunit rRNA sequence followed by HinfI endonuclease restriction. Encephalitozoon intestinalis microsporidiosis was thus shown in two of the four patients examined. In two patients, therapy based on albendazole made stools devoid of microsporidian spores without influence on the intestinal disorders. The pathogenic role of E. intestinalis in immunocompetent individuals remains to be demonstrated.

scholarly journals Role of Era in assembly and homeostasis of the ribosomal small subunit

2019 ◽  
Vol 47 (15) ◽  
pp. 8301-8317 ◽  
Author(s):  
Aida Razi ◽  
Joseph H Davis ◽  
Yumeng Hao ◽  
Dushyant Jahagirdar ◽  
Brett Thurlow ◽  
...  
Keyword(s):  
Ribosomal Subunit ◽  
Small Subunit ◽  
Bacterial Strains ◽  
Quantitative Mass Spectrometry ◽  
Cryo Electron Microscopy ◽  
Escherichia Coli Cells ◽  
Precise Understanding ◽  
30S Subunit ◽  
Assembly Factor

AbstractAssembly factors provide speed and directionality to the maturation process of the 30S subunit in bacteria. To gain a more precise understanding of how these proteins mediate 30S maturation, it is important to expand on studies of 30S assembly intermediates purified from bacterial strains lacking particular maturation factors. To reveal the role of the essential protein Era in the assembly of the 30S ribosomal subunit, we analyzed assembly intermediates that accumulated in Era-depleted Escherichia coli cells using quantitative mass spectrometry, high resolution cryo-electron microscopy and in-cell footprinting. Our combined approach allowed for visualization of the small subunit as it assembled and revealed that with the exception of key helices in the platform domain, all other 16S rRNA domains fold even in the absence of Era. Notably, the maturing particles did not stall while waiting for the platform domain to mature and instead re-routed their folding pathway to enable concerted maturation of other structural motifs spanning multiple rRNA domains. We also found that binding of Era to the mature 30S subunit destabilized helix 44 and the decoding center preventing binding of YjeQ, another assembly factor. This work establishes Era’s role in ribosome assembly and suggests new roles in maintaining ribosome homeostasis.

scholarly journals The Rubisco Small Subunit Is Involved in Tobamovirus Movement and Tm-22-Mediated Extreme Resistance

2012 ◽  
Vol 161 (1) ◽  
pp. 374-383 ◽  
Author(s):  
Jinping Zhao ◽  
Qi Liu ◽  
Haili Zhang ◽  
Qi Jia ◽  
Yiguo Hong ◽  
...  
Keyword(s):  
Small Subunit ◽  
Extreme Resistance ◽  
Rubisco Small Subunit
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