scholarly journals A Long-Time, High Spatiotemporal Resolution Optical Recording System for Membrane Potential Activity via Real-Time Writing to the Hard Disk

2006 ◽  
Vol 56 (3) ◽  
pp. 263-266 ◽  
Author(s):  
Akihiko Hirota ◽  
Shin-ichi Ito
Keyword(s):  
Membrane Potential ◽  
Real Time ◽  
Hard Disk ◽  
Optical Recording ◽  
Recording System ◽  
Spatiotemporal Resolution ◽  
High Spatiotemporal Resolution ◽  
Long Time ◽  
Potential Activity

scholarly journals Micrometer-scale transient ion transport for real-time pH assay in living rat brains

2021 ◽  
Author(s):  
Kailin Zhang ◽  
Huan Wei ◽  
Tianyi Xiong ◽  
Yanan Jiang ◽  
Wenjie Ma ◽  
...  
Keyword(s):  
Ion Transport ◽  
Real Time ◽  
Real Time Monitoring ◽  
Spatiotemporal Resolution ◽  
High Spatiotemporal Resolution ◽  
And Performance ◽  
Micrometer Scale

Micrometer-scale transient ion transport has been successfully used for constructing a high spatiotemporal resolution and performance microsensor, which could be used for real-time monitoring the change of pH in rat brains.

Optical recording of neuronal activity in an invertebrate central nervous system: simultaneous monitoring of several neurons

1977 ◽  
Vol 40 (6) ◽  
pp. 1281-1291 ◽  
Author(s):  
B. M. Salzberg ◽  
A. Grinvald ◽  
L. B. Cohen ◽  
H. V. Davila ◽  
W. N. Ross
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Membrane Potential ◽  
Action Potential ◽  
Neuronal Activity ◽  
Optical Method ◽  
Optical Recording ◽  
Supraesophageal Ganglion ◽  
Potential Activity ◽  
Favorable Conditions

1. Using an optical method for measuring membrane potential, we have been able to monitor action-potential activity simultaneously in 14 neurons of the supraesophageal ganglion of the barnacle. 2. Under favorable conditions, 4-mV synaptic potentials could also be detected optically.

scholarly journals Motorized Treadmill and Optical Recording System for Gait Analysis of Grasshoppers

Sensors ◽  
2021 ◽  
Vol 21 (17) ◽  
pp. 5953
Author(s):  
Leslie Barreto ◽  
Ahnsei Shon ◽  
Derrick Knox ◽  
Hojun Song ◽  
Hangue Park ◽  
...  
Keyword(s):  
Pilot Study ◽  
Gait Analysis ◽  
Real Time ◽  
Closed Loop ◽  
Walking Speed ◽  
Optical Recording ◽  
Recording System ◽  
Model Systems ◽  
Gait Kinematics ◽  
Quantitative Gait Analysis

(1) Background: Insects, which serve as model systems for many disciplines with their unique advantages, have not been extensively studied in gait research because of the lack of appropriate tools and insect models to properly study the insect gaits. (2) Methods: In this study, we present a gait analysis of grasshoppers with a closed-loop custom-designed motorized insect treadmill with an optical recording system for quantitative gait analysis. We used the eastern lubber grasshopper, a flightless and large-bodied species, as our insect model. Gait kinematics were recorded and analyzed by making three grasshoppers walk on the treadmill with various speeds from 0.1 to 1.5 m/s. (3) Results: Stance duty factor was measured as 70–95% and decreased as walking speed increased. As the walking speed increased, the number of contact legs decreased, and diagonal arrangement of contact was observed at walking speed of 1.1 cm/s. (4) Conclusions: This pilot study of gait analysis of grasshoppers using the custom-designed motorized insect treadmill with the optical recording system demonstrates the feasibility of quantitative, repeatable, and real-time insect gait analysis.

scholarly journals Optical recording of membrane potential activity

1999 ◽  
Vol 20 (4) ◽  
pp. 391-403
Author(s):  
Kohtaro Kamino ◽  
Yoko Momose-Sato ◽  
Katsushige Sato
Keyword(s):  
Membrane Potential ◽  
Optical Recording ◽  
Potential Activity

scholarly journals Difluoroboron β-diketonate materials with long-lived phosphorescence enable lifetime based oxygen imaging with a portable cost effective camera

2016 ◽  
Vol 8 (15) ◽  
pp. 3109-3114 ◽  
Author(s):  
Alexander S. Mathew ◽  
Christopher A. DeRosa ◽  
James N. Demas ◽  
Cassandra L. Fraser
Keyword(s):  
Real Time ◽  
Imaging System ◽  
Oxygen Sensing ◽  
Cost Effective ◽  
Cmos Camera ◽  
Spatiotemporal Resolution ◽  
Oxygen Imaging ◽  
High Spatiotemporal Resolution ◽  
System P

Real-time oxygen sensing with high spatiotemporal resolution was achieved with a luminescent boron dye-polylactide CMOS camera laptop imaging system.

Fluorescent potentiometric dyes for membrane-potential imaging

1994 ◽  
Vol 52 ◽  
pp. 166-167
Author(s):  
Leslie M. Loew
Keyword(s):  
Membrane Potential ◽  
Single Cells ◽  
Quantitative Imaging ◽  
Optical Recording ◽  
Biological Processes ◽  
Major Focus ◽  
The Past ◽  
Excitable Systems ◽  
Major Application ◽  
The Impact

A major application of potentiometric dyes has been the multisite optical recording of electrical activity in excitable systems. After being championed by L.B. Cohen and his colleagues for the past 20 years, the impact of this technology is rapidly being felt and is spreading to an increasing number of neuroscience laboratories. A second class of experiments involves using dyes to image membrane potential distributions in single cells by digital imaging microscopy - a major focus of this lab. These studies usually do not require the temporal resolution of multisite optical recording, being primarily focussed on slow cell biological processes, and therefore can achieve much higher spatial resolution. We have developed 2 methods for quantitative imaging of membrane potential. One method uses dual wavelength imaging of membrane-staining dyes and the other uses quantitative 3D imaging of a fluorescent lipophilic cation; the dyes used in each case were synthesized for this purpose in this laboratory.

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