scholarly journals Novel Inverted Triple Grease-Gap Isolation Chamber for Electrophysiological and Calcium Release Studies in Skeletal Muscle Fibers.

2000 ◽  
Vol 50 (4) ◽  
pp. 457-462 ◽  
Author(s):  
Marbella Quiñonez ◽  
Marino DiFranco
Keyword(s):  
Skeletal Muscle ◽  
Calcium Release ◽  
Muscle Fibers ◽  
Isolation Chamber ◽  
Skeletal Muscle Fibers ◽  
Release Studies

scholarly journals Growth Associated Protein 43 Is Expressed in Skeletal Muscle Fibers and Is Localized in Proximity of Mitochondria and Calcium Release Units

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e53267 ◽  
Author(s):  
Simone Guarnieri ◽  
Caterina Morabito ◽  
Cecilia Paolini ◽  
Simona Boncompagni ◽  
Raffaele Pilla ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Calcium Release ◽  
Muscle Fibers ◽  
Skeletal Muscle Fibers

scholarly journals Spontaneous and Voltage-Activated Elementary Calcium Release Events in Intact Skeletal Muscle Fibers Expressing the Embryonic CaV1.1 Splice Variant

2016 ◽  
Vol 110 (3) ◽  
pp. 97a
Author(s):  
Beatrix Dienes ◽  
János Vincze ◽  
Péter Szentesi ◽  
Nasreen Sultana ◽  
Berhnard E. Flucher ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Splice Variant ◽  
Calcium Release ◽  
Muscle Fibers ◽  
Skeletal Muscle Fibers

Calsequestrin targeting to sarcoplasmic reticulum of skeletal muscle fibers

2006 ◽  
Vol 291 (2) ◽  
pp. C245-C253 ◽  
Author(s):  
Alessandra Nori ◽  
Giorgia Valle ◽  
Elena Bortoloso ◽  
Federica Turcato ◽  
Pompeo Volpe
Keyword(s):  
Skeletal Muscle ◽  
Sarcoplasmic Reticulum ◽  
Calcium Binding ◽  
Calcium Release ◽  
Structural Information ◽  
Muscle Fibers ◽  
Physiological Role ◽  
Homogeneous Distribution ◽  
Skeletal Muscle Fibers ◽  
Domain Iii

Calsequestrin (CS) is the low-affinity, high-capacity calcium binding protein segregated to the lumen of terminal cisternae (TC) of the sarcoplasmic reticulum (SR). The physiological role of CS in controlling calcium release from the SR depends on both its intrinsic properties and its localization. The mechanisms of CS targeting were investigated in skeletal muscle fibers and C2C12 myotubes, a model of SR differentiation, with four deletion mutants of epitope (hemagglutinin, HA)-tagged CS: CS-HAΔ24NH2, CS-HAΔ2D, CS-HAΔ3D, and CS-HAΔHT, a double mutant of the NH2 terminus and domain III. As judged by immunofluorescence of transfected skeletal muscle fibers, only the double CS-HA mutant showed a homogeneous distribution at the sarcomeric I band, i.e., it did not segregate to TC. As shown by subfractionation of microsomes derived from transfected skeletal muscles, CS-HAΔHT was largely associated to longitudinal SR whereas CS-HA was concentrated in TC. In C2C12 myotubes, as judged by immunofluorescence, not only CS-HAΔHT but also CS-HAΔ3D and CS-HAΔ2D were not sorted to developing SR. Condensation competence, a property referable to CS oligomerization, was monitored for the several CS-HA mutants in C2C12 myoblasts, and only CS-HAΔ3D was found able to condense. Together, the results indicate that 1) there are at least two targeting sequences at the NH2 terminus and domain III of CS, 2) SR-specific target and structural information is contained in these sequences, 3) heterologous interactions with junctional SR proteins are relevant for segregation, 4) homologous CS-CS interactions are involved in the overall targeting process, and 5) different targeting mechanisms prevail depending on the stage of SR differentiation.

scholarly journals Measurement of force and calcium release using mechanically skinned fibers from mammalian skeletal muscle

2018 ◽  
Vol 125 (4) ◽  
pp. 1105-1127 ◽  
Author(s):  
Graham D. Lamb ◽  
D. George Stephenson
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fiber ◽  
Calcium Release ◽  
Muscle Fibers ◽  
Mammalian Skeletal Muscle ◽  
Ec Coupling ◽  
Skeletal Muscle Fibers ◽  
Intact Muscle ◽  
Key Variables ◽  
Coupling Sequence

The mechanically skinned (or “peeled”) skeletal muscle fiber technique is a highly versatile procedure that allows controlled examination of each of the steps in the excitation-contraction (EC)-coupling sequence in skeletal muscle fibers, starting with excitation/depolarization of the transverse tubular (T)-system through to Ca2+ release from sarcoplasmic reticulum (SR) and finally force development by the contractile apparatus. It can also show the overall response of the whole EC-coupling sequence together, such as in twitch and tetanic force responses. A major advantage over intact muscle fiber preparations is that it is possible to set and rapidly manipulate the “intracellular” conditions, allowing examination of the effects of key variables (e.g., intracellular pH, ATP levels, redox state, etc.) on each individual step in EC coupling. This Cores of Reproducibility in Physiology (CORP) article describes the rationale, procedures, and experimental details of the various ways in which the mechanically skinned fiber technique is used in our laboratory to examine the physiological mechanisms controlling Ca2+ release and contraction in skeletal muscle fibers and the aberrations and dysfunction occurring with exercise and disease.

scholarly journals Time course of calcium release and removal in skeletal muscle fibers

1984 ◽  
Vol 45 (3) ◽  
pp. 637-641 ◽  
Author(s):  
W. Melzer ◽  
E. Rios ◽  
M.F. Schneider
Keyword(s):  
Skeletal Muscle ◽  
Time Course ◽  
Calcium Release ◽  
Muscle Fibers ◽  
Skeletal Muscle Fibers

scholarly journals Action Potential-Evoked Calcium Release Is Impaired in Single Skeletal Muscle Fibers from Heart Failure Patients

PLoS ONE ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. e109309 ◽  
Author(s):  
Marino DiFranco ◽  
Marbella Quiñonez ◽  
Perry Shieh ◽  
Gregg C. Fonarow ◽  
Daniel Cruz ◽  
...  
Keyword(s):  
Heart Failure ◽  
Skeletal Muscle ◽  
Action Potential ◽  
Calcium Release ◽  
Muscle Fibers ◽  
Heart Failure Patients ◽  
Skeletal Muscle Fibers
Sign in / Sign up

Export Citation Format

Share Document