scholarly journals Involvement of Ca2+ jn Antiarrhythmic Effect of Ischemic Preconditioning in Isolated Rat Heart.

2000 ◽  
Vol 50 (2) ◽  
pp. 207-213 ◽  
Author(s):  
Kui Hong ◽  
Kengo Fukushima Kusano ◽  
Hiroshi Morita ◽  
Yoshihisa Fujimoto ◽  
Kazufumi Nakamura ◽  
...  
Keyword(s):  
Ischemic Preconditioning ◽  
Rat Heart ◽  
Isolated Rat Heart ◽  
Antiarrhythmic Effect ◽  
Isolated Rat

scholarly journals Contribution of endocannabinoids in the endothelial protection afforded by ischemic preconditioning in the isolated rat heart

Life Sciences ◽  
2003 ◽  
Vol 72 (16) ◽  
pp. 1859-1870 ◽  
Author(s):  
Jean-François Bouchard ◽  
Philippe Lépicier ◽  
Daniel Lamontagne
Keyword(s):  
Ischemic Preconditioning ◽  
Rat Heart ◽  
Isolated Rat Heart ◽  
Isolated Rat

Ischemic preconditioning translocates PKC-δ and -ε, which mediate functional protection in isolated rat heart

1998 ◽  
Vol 275 (6) ◽  
pp. H2266-H2271 ◽  
Author(s):  
Shuji Kawamura ◽  
Ken-Ichi Yoshida ◽  
Toshiro Miura ◽  
Yoichi Mizukami ◽  
Masunori Matsuzaki
Keyword(s):  
Cardiac Function ◽  
Ischemic Preconditioning ◽  
Rat Heart ◽  
Membrane Fraction ◽  
Isolated Rat Heart ◽  
Kinase C ◽  
Rat Hearts ◽  
Pkc Isoforms ◽  
The Relationship ◽  
Isolated Rat

Protein kinase C (PKC) plays an important role in mediating ischemic preconditioning (PC). However, the relationship between PKC isoforms and PC is still uncertain. We analyzed subcellular localization of PKC isoforms by Western blot analysis in isolated rat heart and demonstrate that PKC-α, -δ, and -ε were translocated to the membrane fraction associated with the improvement of cardiac function. Translocation of PKC-δ and -ε persisted after a 30-min period following PC, but the translocation of PKC-α was transient. Under low Ca2+ perfusion (0.2 mmol/l), PC improved the cardiac function associated with the translocation of PKC-δ. Chelerythrine (1.0 μmol/l) suppressed the translocation of all PKC isoforms associated with the loss of improvement of the cardiac function. On the other hand, bisindolylmaleimide (0.1 μmol/l) did not inhibit the improvement of cardiac function induced by PC, which was associated with the translocation of PKC-ε. These results indicate that the effect of PC on cardiac function is mediated by the translocation of either PKC-δ or -ε independently in rat hearts.

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