scholarly journals Protein Kinase C Inhibitors Abolish the Increased Resistance of Diabetic Rat Heart to Ischemia-Reperfusion Injury.

1999 ◽  
Vol 49 (5) ◽  
pp. 409-415 ◽  
Author(s):  
Chang-Hyun MOON ◽  
Yi-Sook JUNG ◽  
Soo Hwan LEE ◽  
Eun Joo BAIK
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Reperfusion Injury ◽  
Rat Heart ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Diabetic Rat ◽  
Protein Kinase C Inhibitors ◽  
Kinase C

Intermittent hypoxia protects the rat heart against ischemia/reperfusion injury by activating protein kinase C

Life Sciences ◽  
2004 ◽  
Vol 75 (21) ◽  
pp. 2587-2603 ◽  
Author(s):  
Hai-Lei Ding ◽  
Hai-Feng Zhu ◽  
Jian-Wen Dong ◽  
Wei-Zhong Zhu ◽  
Zhao-Nian Zhou
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Reperfusion Injury ◽  
Rat Heart ◽  
Intermittent Hypoxia ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Kinase C

scholarly journals Cardiac Ischemic Preconditioning Promotes MG53 Secretion Through H 2 O 2 -Activated Protein Kinase C-δ Signaling

Circulation ◽  
2020 ◽  
Vol 142 (11) ◽  
pp. 1077-1091
Author(s):  
Dan Shan ◽  
Sile Guo ◽  
Hong-Kun Wu ◽  
Fengxiang Lv ◽  
Li Jin ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Underlying Mechanism ◽  
Cardiac Ischemia ◽  
Kinase C ◽  
Perfused Hearts

Background: Ischemic heart disease is the leading cause of morbidity and mortality worldwide. Ischemic preconditioning (IPC) is the most powerful intrinsic protection against cardiac ischemia/reperfusion injury. Previous studies have shown that a multifunctional TRIM family protein, MG53 (mitsugumin 53; also called TRIM72), not only plays an essential role in IPC-mediated cardioprotection against ischemia/reperfusion injury but also ameliorates mechanical damage. In addition to its intracellular actions, as a myokine/cardiokine, MG53 can be secreted from the heart and skeletal muscle in response to metabolic stress. However, it is unknown whether IPC-mediated cardioprotection is causally related to MG53 secretion and, if so, what the underlying mechanism is. Methods: Using proteomic analysis in conjunction with genetic and pharmacological approaches, we examined MG53 secretion in response to IPC and explored the underlying mechanism using rodents in in vivo, isolated perfused hearts, and cultured neonatal rat ventricular cardiomyocytes. Moreover, using recombinant MG53 proteins, we investigated the potential biological function of secreted MG53 in the context of IPC and ischemia/reperfusion injury. Results: We found that IPC triggered robust MG53 secretion in rodents in vivo, perfused hearts, and cultured cardiac myocytes without causing cell membrane leakage. Mechanistically, IPC promoted MG53 secretion through H 2 O 2 -evoked activation of protein kinase-C-δ. Specifically, IPC-induced myocardial MG53 secretion was mediated by H 2 O 2 -triggered phosphorylation of protein kinase-C-δ at Y311, which is necessary and sufficient to facilitate MG53 secretion. Functionally, systemic delivery of recombinant MG53 proteins to mimic elevated circulating MG53 not only restored IPC function in MG53-deficient mice but also protected rodent hearts from ischemia/reperfusion injury even in the absence of IPC. Moreover, oxidative stress by H 2 O 2 augmented MG53 secretion, and MG53 knockdown exacerbated H 2 O 2 -induced cell injury in human embryonic stem cell–derived cardiomyocytes, despite relatively low basal expression of MG53 in human heart. Conclusions: We conclude that IPC and oxidative stress can trigger MG53 secretion from the heart via an H 2 O 2 –protein kinase-C-δ–dependent mechanism and that extracellular MG53 can participate in IPC protection against cardiac ischemia/reperfusion injury.

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