scholarly journals Inwardly Rectifying K+ Channel in Retinal Mueller Cells: Comparison with the KAB-2/Kir4.1 Channel Expressed in HEK293T Cells.

1998 ◽  
Vol 48 (1) ◽  
pp. 71-80 ◽  
Author(s):  
Yoshihiko TADA ◽  
Yoshiyuki HORIO ◽  
Yoshihisa KURACHI
Keyword(s):  
K Channel ◽  
Mueller Cells ◽  
Inwardly Rectifying

scholarly journals Functional expression of a vertebrate inwardly rectifying K+ channel in yeast.

1995 ◽  
Vol 6 (9) ◽  
pp. 1231-1240 ◽  
Author(s):  
W Tang ◽  
A Ruknudin ◽  
W P Yang ◽  
S Y Shaw ◽  
A Knickerbocker ◽  
...  
Keyword(s):  
Transcriptional Control ◽  
Xenopus Oocytes ◽  
Genetic System ◽  
Functional Expression ◽  
Inward Rectifier ◽  
K Channel ◽  
Coding Region ◽  
Low Potassium ◽  
K Current ◽  
Inwardly Rectifying

We describe the expression of gpIRK1, an inwardly rectifying K+ channel obtained from guinea pig cardiac cDNA. gpIRK1 is a homologue of the mouse IRK1 channel identified in macrophage cells. Expression of gpIRK1 in Xenopus oocytes produces inwardly rectifying K+ current, similar to the cardiac inward rectifier current IK1. This current is blocked by external Ba2+ and Cs+. Plasmids containing the gpIRK1 coding region under the transcriptional control of constitutive (PGK) or inducible (GAL) promoters were constructed for expression in Saccharomyces cerevisiae. Several observations suggest that gpIRK1 forms functional ion channels when expressed in yeast. gpIRK1 complements a trk1 delta trk2 delta strain, which is defective in potassium uptake. Expression of gpIRK1 in this mutant restores growth on low potassium media. Growth dependent on gpIRK1 is inhibited by external Cs+. The strain expressing gpIRK1 provides a versatile genetic system for studying the assembly and composition of inwardly rectifying K+ channels.

scholarly journals Imaging ROMK1 inwardly rectifying ATP-sensitive K+ channel protein using atomic force microscopy.

1996 ◽  
Vol 93 (16) ◽  
pp. 8756-8760 ◽  
Author(s):  
R. M. Henderson ◽  
S. Schneider ◽  
Q. Li ◽  
D. Hornby ◽  
S. J. White ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
K Channel ◽  
Force Microscopy ◽  
Channel Protein ◽  
Atomic Force ◽  
Inwardly Rectifying
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