scholarly journals Positive Inotropic, Negative Chronotropic, and Coronary Vasoconstrictor Effects of Acetylcholine in Isolated Rat Hearts: Role of Muscarinic Receptors, Prostaglandins, Protein Kinase C, Influx of Extracellular Ca2+, Intracellular Ca2+ Release, and Endothelium.

1998 ◽  
Vol 48 (6) ◽  
pp. 483-491 ◽  
Author(s):  
Sadiye ATES ◽  
Ziya KAYGISIZ
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Muscarinic Receptors ◽  
Kinase C ◽  
Rat Hearts ◽  
Isolated Rat ◽  
Coronary Vasoconstrictor

scholarly journals The Role of Protein Kinase C in Ischemic/Reperfused Preconditioned Isolated Rat Hearts

1996 ◽  
Vol 28 (5) ◽  
pp. 723-731 ◽  
Author(s):  
Arpad Tosaki ◽  
Nilanjana Maulik ◽  
Daniel T. Engelman ◽  
Richard M. Engelman ◽  
Dipak K. Das
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Kinase C ◽  
Rat Hearts ◽  
Isolated Rat

scholarly journals Autophagy and protein kinase C are required for cardioprotection by sulfaphenazole

2010 ◽  
Vol 298 (2) ◽  
pp. H570-H579 ◽  
Author(s):  
Chengqun Huang ◽  
Wayne Liu ◽  
Cynthia N. Perry ◽  
Smadar Yitzhaki ◽  
Youngil Lee ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Enhanced Recovery ◽  
Ischemia Reperfusion ◽  
Dominant Negative ◽  
Rat Cardiomyocytes ◽  
Adult Rat ◽  
Kinase C ◽  
Rat Hearts

Previously, we showed that sulfaphenazole (SUL), an antimicrobial agent that is a potent inhibitor of cytochrome P4502C9, is protective against ischemia-reperfusion (I/R) injury (Ref. 15 ). The mechanism, however, underlying this cardioprotection, is largely unknown. With evidence that activation of autophagy is protective against simulated I/R in HL-1 cells, and evidence that autophagy is upregulated in preconditioned hearts, we hypothesized that SUL-mediated cardioprotection might resemble ischemic preconditioning with respect to activation of protein kinase C and autophagy. We used the Langendorff model of global ischemia to assess the role of autophagy and protein kinase C in myocardial protection by SUL during I/R. We show that SUL enhanced recovery of function, reduced creatine kinase release, decreased infarct size, and induced autophagy. SUL also triggered PKC translocation, whereas inhibition of PKC with chelerythrine blocked the activation of autophagy in adult rat cardiomyocytes. In the Langendorff model, chelerythrine suppressed autophagy and abolished the protection mediated by SUL. SUL increased autophagy in adult rat cardiomyocytes infected with GFP-LC3 adenovirus, in isolated perfused rat hearts, and in mCherry-LC3 transgenic mice. To establish the role of autophagy in cardioprotection, we used the cell-permeable dominant-negative inhibitor of autophagy, Tat-Atg5K130R. Autophagy and cardioprotection were abolished in rat hearts perfused with recombinant Tat-Atg5K130R. Taken together, these studies indicate that cardioprotection mediated by SUL involves a PKC-dependent induction of autophagy. The findings suggest that autophagy may be a fundamental process that enhances the heart's tolerance to ischemia.

The role of protein kinase C in the increased generation in isolated rat hepatocytes of the hydroxyl radical by puromycin aminonucleoside

2000 ◽  
Vol 32 (6) ◽  
pp. 487-496 ◽  
Author(s):  
Kazumasa Aoyagi ◽  
Siranoush Shahrzad ◽  
Yutaka Kuzure ◽  
Akio Koyama ◽  
Ko Nakamura ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Hydroxyl Radical ◽  
Rat Hepatocytes ◽  
Puromycin Aminonucleoside ◽  
Isolated Rat Hepatocytes ◽  
Kinase C ◽  
Isolated Rat

Investigation of the role of protein kinase C inhibition in the calcium independent relaxant effects of oestrogens on isolated rat aorta

1996 ◽  
Vol 24 (3) ◽  
pp. 377S-377S ◽  
Author(s):  
HOSSEIN BABAEI ◽  
A. TUDOR EVANS ◽  
GRAHAM IRVING ◽  
JANICE R. McCURRIE
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Rat Aorta ◽  
Kinase C ◽  
Isolated Rat
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