scholarly journals A simple microtonometric method for whole blood oxygen dissociation curve and a critical evaluation of the "single point" procedure for blood P50.

1983 ◽  
Vol 33 (6) ◽  
pp. 987-994 ◽  
Author(s):  
Hisaharu KOHZUKI ◽  
Yasunori ENOKI ◽  
Susumu SAKATA ◽  
Susumu TOMITA
Keyword(s):  
Whole Blood ◽  
Single Point ◽  
Critical Evaluation ◽  
Blood Oxygen ◽  
Oxygen Dissociation Curve ◽  
Dissociation Curve ◽  
Oxygen Dissociation

MODIFICATION OF THE HAEMOGLOBIN OXYGEN DISSOCIATION CURVE IN WHOLE BLOOD BY A COMPOUND WITH DUAL ACTION

The Lancet ◽  
1984 ◽  
Vol 324 (8393) ◽  
pp. 15-16 ◽  
Author(s):  
R.M. Hyde ◽  
D.J. Livingstone ◽  
R.A. Patterson ◽  
J.F. Batchelor ◽  
W.R. King
Keyword(s):  
Whole Blood ◽  
Oxygen Dissociation Curve ◽  
Dissociation Curve ◽  
Oxygen Dissociation ◽  
Dual Action

In vivo Blood Oxygen Dissociation Curve of Newborn Infants

Neonatology ◽  
1965 ◽  
Vol 8 (5-6) ◽  
pp. 241-252 ◽  
Author(s):  
W. Oh ◽  
R.A. Arcilla ◽  
J. Lind
Keyword(s):  
Newborn Infants ◽  
Blood Oxygen ◽  
Oxygen Dissociation Curve ◽  
Dissociation Curve ◽  
Oxygen Dissociation

scholarly journals Continuous Determination of the Oxygen Dissociation Curve for Whole Blood

1975 ◽  
Vol 21 (12) ◽  
pp. 1747-1753 ◽  
Author(s):  
Luigi Rossi-Bernardi ◽  
Massimo Luzzana ◽  
Michele Samaja ◽  
Mario Davi ◽  
Daniela DaRiva-Ricci ◽  
...  
Keyword(s):  
Oxygen Saturation ◽  
Whole Blood ◽  
Circulatory System ◽  
Oxygen Electrode ◽  
Oxygen Dissociation Curve ◽  
Dissociation Curve ◽  
Total Oxygen ◽  
Oxygen Dissociation ◽  
Continuous Determination

Abstract We report here the development of a new method that allows continuous determination of the oxygen dissociation curve for microsamples (600 µl) of whole blood under conditions of pH, pco2, methemoglobin concentration, and 2,3-diphosphoglycerate content closely approaching those found in the circulatory system. The method consists of gradually oxygenating a blood sample by adding H2O2 in the presence of catalase (EC 1.11.1.6), to produce the reaction H2O2 → H2O + ½ O2. Because the total oxygen content of blood can be derived from the known rate of H2O2 addition and the po2 is determined in the liquid phase by an oxygen electrode, the two functions (total O2 content) and (% oxygen saturation) vs. po2 are simple to calculate. pco2 and pH are controlled by adding base simultaneously with the gradual oxygenation of blood. The method described thus avoids the direct measurement of oxygen saturation of whole blood.

Oxygen dissociation curves for whole blood, recorded with an instrument that continuously measures pO2 and sO2 independently at constant t, pCO2, and pH.

1982 ◽  
Vol 28 (6) ◽  
pp. 1287-1292 ◽  
Author(s):  
A Zwart ◽  
G Kwant ◽  
B Oeseburg ◽  
W G Zijlstra
Keyword(s):  
Whole Blood ◽  
Data Acquisition System ◽  
Oxygen Dissociation Curve ◽  
Dissociation Curve ◽  
Measuring Chamber ◽  
Oxygen Dissociation ◽  
Constant Determination ◽  
Blood Specimens ◽  
Dissociation Curves

Abstract We describe a method for recording oxygen dissociation curves for whole-blood specimens. The blood sample is placed in a thermostated measuring chamber, and pO2 and SO2 are measured continuously by polarography and by reflectometry, respectively. During the recording of an oxygen dissociation curve, the pO2 and SO2 signals are stored in a data-acquisition system, while pH, pCO2, and temperature are kept constant. Determination of precision and error discussion indicated that the coefficient of variation (CV) of the determination of the oxygen dissociation curve is mainly determined by the error in the measurement of SO2. The overall CV of pO2 values belonging to the lower, mid-, and upper parts of the SO2 range is estimated to be about 2.6, 3.1, and 2.1%, respectively. In practice the measurements are about 30% more precise than estimated. With our method, the fixed-acid-induced Bohr effect (H+ factor) can be determined over the entire SO2 range with much greater precision than hitherto.

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