scholarly journals Ranga bioindykacji w ekotoksykologii wód

2012 ◽  
Vol 10 (2) ◽  
pp. 33-52 ◽  
Author(s):  
Urszula Sadowska
Keyword(s):  
Surface Water ◽  
Biological Monitoring ◽  
Aquatic Ecosystems ◽  
Ecological Status ◽  
Standard Test ◽  
Monitoring Systems ◽  
Long Time ◽  
Test Organisms ◽  
Bioindication Methods

Bioindication methods are applied in ecotoxicological studies which in turn are widely used in the biological monitoring of surface water. Bioindication has been applied for a long time and so far it has created multiple classificationsbased on diagnosis groups of organisms. Bioindicators play a signi]cant role in bioindication; they are species used to monitor the health of the environment or ecosystem. Some of them are standard test organisms used in ecotoxicologyin the so-called bioassays, which provide a great deal of information about the type and in^uence of pollution. These studies are vital nowadays, as compounds have been found to be the greatest threat to aquatic ecosystems. In relation to ecotoxicology, research allows us to determine bioconcentration, bioaccumulation and biomagni]cation factors, and to assess the ecological status of water used in many different indexes so as to determine such relevant parameters as saprobic, biological bio-diversity and biotic indexes. Poland is preparing for the extension of monitoring systems that take into account all the necessary elements of the environment, even the sediments. It is required by the Water Frame Directive.

Diatom biomonitoring – scientific foundations, commonly discussed issues and frequently made errors

2018 ◽  
Vol 47 (3) ◽  
pp. 313-325 ◽  
Author(s):  
Ewelina Szczepocka ◽  
Joanna Żelazna-Wieczorek
Keyword(s):  
Water Quality ◽  
Surface Water ◽  
Aquatic Ecosystems ◽  
Objective Assessment ◽  
Ecological Status ◽  
Biological Assessment ◽  
Basic Tool ◽  
Diatom Indices ◽  
The World ◽  
Made In

Abstract Contemporary assessment of the ecological status of aquatic ecosystems is based on various groups of organisms, including diatoms. Biological assessment, implemented by the Water Framework Directive, has been applied worldwide for more than 15 years. Currently, the most basic “tool” used in biomonitoring are diatom indices, which are routinely applied in Europe and other countries around the world. In Poland, the diatom indices have been used for over 5 years, which is a period of time allowing to summarize and evaluate the effectiveness of these methods in the assessment of aquatic ecosystems. The purpose of this work was to analyse the use of diatom indices in the biomonitoring of surface water by collecting data on the possibilities and limitations of using diatom indices for the objective assessment of water quality. Attention was paid to mistakes made in the course of biological assessment performed with the use of diatom phytobenthos, which have a significant impact on the obtained results. The paper also contains suggestions for introducing several important changes in biological monitoring, which will improve its quality and efficiency in assessing the ecological status of various aquatic ecosystems.

On methodological approaches to the improvement of surface water bodies and target indicators of the natural waters’ quality

2021 ◽  
Vol 27 (1) ◽  
pp. 6-14
Author(s):  
V. Zaslonovsky ◽  
◽  
N. Sharapov ◽  
M. Bosov ◽  
◽  
...  
Keyword(s):  
Water Quality ◽  
Surface Water ◽  
Natural Water ◽  
Aquatic Ecosystems ◽  
Natural Waters ◽  
Water Bodies ◽  
Surface Water Bodies ◽  
Methodological Approaches ◽  
Natural Water Bodies

The paper is devoted to the development of proposals concerning the improvement of surface water bodies and the appointment of target indicators of the natural waters’ quality. The paper considers a variety of problems of preserving, restoring and improving the quality of natural surface waters: from regional features of the formation of natural water composition, to differences in the requirements for the quality of water consumed. The object of the study is the qualitative indicators of the waters of natural water bodies, and the subject of the study is the methodological approaches to the normalization of these indicators. The purpose of the work is to identify the main shortcomings in the domestic system of rationing the quality of natural waters, and to form appropriate proposals. For this purpose, the following tasks were set and solved: to review the methodological approaches to assessing and rationing the quality of natural waters used in some foreign countries and in the Russian Federation, to identify the main causes and shortcomings; to make proposals for improving the current system of water quality rationing. The result of this work is the conclusions about the lack of impact of the strategy operating in Russia in the development of schemes of complex use and protection of water objects, standards of permissible impact on water bodies, the appointment of permissible discharge limits in terms of maintaining and improving the water quality of natural water bodies. The reasons that led to this are indicated. The necessity of taking into account, in addition to the physical and chemical composition, also hydrobiological parameters is shown. It is concluded that instead of fisheries management standards, environmental standards should serve as the basis for target indicators of water quality of natural water bodies, which determine the well-being of humans and the stable functioning of aquatic ecosystems, taking into account regional factors. Proposals were made to adjust the methodological approaches to the implementation of this strategy. According to the authors, these proposals will speed up the solution of the main objectives of the strategy – the ecological improvement of water bodies, the preservation of unique aquatic ecosystems and the environmentally safe development of territories that previously experienced relatively small anthropogenic impacts (Eastern Siberia and the Far East)

SENSITIVITY OF FORSYTHIA POLLEN TO THE FACTORS OF THE ENVIRONMENT OF THE MEGAPOLIS

2021 ◽  
pp. 56-74
Author(s):  
Matiashuk R. ◽  
Tkachenko I.
Keyword(s):  
Air Pollution ◽  
Ecological Status ◽  
Pollution Index ◽  
Weather Conditions ◽  
Botanical Garden ◽  
Control Value ◽  
Adverse Weather ◽  
Long Time ◽  
Growing Conditions ◽  
High Level

The sensitivity of the reproductive structures of Forsythiasuspensato the complex influence of undifferentiated environmental factors has been studied.The monitoring sites are located in 15 different park ecosystems in 6 administrative districts of Kyiv. Data from the Borys Sreznevsky Central Geophysical Observatory (air pollution index (API) and meteorological conditions for 2018-2020) were used to assess the conditions of the growthenvironment. The influence of a complex of ecologically important factors during the flowering offorsythia on the quality of the formedpollen is noted. Thus, growing plantsfor a long time in conditions with a low level of air pollution (APIup to 5.0) in abnormal weather conditions in 2020,40-50% less fertile grainswere formed. And forplants, which grow in areas with increasedlevel (API5.0-7.0) and high level (API7.0-14.0) of air pollution, the share of fertile grains in the pollen population decreased by 60-80%. In the closed bud,the pollen has higher resistance to a complex of exogenous growth factors. Forsythia plants, which are located in large parklands, lose less pollen quality in adverse weather conditions and affected byurbotechnogenic factors. F. suspensa is an acceptable indicator of the level of environmental pollution by the deviation of pollen fertility from the control value. In areas with high aerogenic load, for example, areas with large highways (Bus Station «Darnytsia»), as well as with a significant recreational load (HolosiivskyiPark, Recreation Park on the Olena Teliha Street) much smaller pollen is formed. Itis noted that the conditions of forsythia growth affect the quantitative indicators of the formed pollen not only in the flower but also in the closed bud, which confirms the chronic effect of the complex of ingredients of aerotechnogenic emissions on plants of this species. The coefficient of sterility of pollen (CS) was used to objectively compare the data of 2019 and 2020 on the condition of the generative organs of F. suspensa in the studied areas. The calculation of the CS confirmed that in the closedbud pollen has a higher resistance to exogenous factors. In areas with high and increased levels of air pollution,during budding and flowering of plants (March-May,) there is a much higher CS of pollen of F. suspensа. Significant parkland territoriesof the city (for example, HolosiivskyiPark, Botanical Garden named after O. V. Fomin) provide less stressful conditions for growth and development of plants, even with the "very high" level of air pollution (ISA above 14.0) observed in April 2020 on this territory.The study of the susceptibility of F. suspensаpollen to growing conditions will be continued, as the prospects of using this species for bioindication of ecological status and zoning of park ecosystems of Kyiv according to the gradient of anthropogenic impact have been revealed.Key words:forsythia, fertility, coefficient of sterility of pollen, bioindication. Проведене дослідження чутливості репродуктивних структур Forsythiasuspensaдо комплексного впливу недиференційованих факторів навколишнього середовища. Моніторингові ділянки розташовані в 15 різних паркових екосистемах 6 адміністративних районівКиєва. Для оцінки умов середовища вирощування рослин використані дані Центральної геофізичної обсерваторії імені Бориса Срезневського (індекс забруднення атмосферного повітря (ІЗА) та метеорологічні умови за 2018-2020 рр.). Відмічено вплив комплексу екологічно важливих факторів (за показниками відхилення від норми середньої місячної температури повітря та місячної кількості опадів у Києві) уперіод квітування форзиції на якість сформованого пилку. Так, за тривалої експозиції рослин в умовах з низьким рівнем забруднення повітря (ІЗА до 5,0) в аномальних погодних умовах 2020 р. сформувалось на 40-50% менше фертильних зерен. А у форзиції, яка росте на територіях з підвищеним (ІЗА 5,0-7,0) та високим (ІЗА 7,0-14,0) рівнями забруднення частка фертильних зерен в популяції пилку зменшилась на 60-80%. У закритому бутоні пилок має вищу стійкість до комплексу екзогенних факторів середовища зростання. Рослини форзиції, які розташовані у значних за площею паркових насадженнях, менше втрачають якість пилку за несприятливих погодних умов та дії урботехногенних чинників. За відхиленням показника фертильності пилку від контрольного значення F. suspensaє допустимим індикатором рівня забруднення середовища. На територіях з підвищеним аерогенним навантаженням, наприклад, ділянки з автотранспортними магістралями (Автостанція «Дарниця»), а також зі значним рекреаційним навантаженням (Голосіївський парк імені М. Рильського, Парк відпочинку по вул. Олени Теліги)формується значно дрібніший пилок. Відмічено, що умови росту позначаються на кількісних показниках сформованого пилку не лише в квітці, але й в закритому бутоні, що підтверджує хронічний вплив комплексу інгредієнтів аеротехногенних викидів нарослини цього виду. Для об’єктивного співставлення даних 2019 і 2020 рр. щодо стану генеративних органів F. suspensaна досліджених ділянках був використаний коефіцієнт стерильності (КС) пилку. Розрахунок КС підтвердив, що в закритому бутоні пилок має вищу стійкість до впливу екзогенних чинників. Вищий КС був у F. suspense, з ділянок, на яких в період бутонізації і квітування рослин (березень-травень) відмічений високий і підвищений рівень забруднення атмосфери. Значні за площею паркові насадження міста (наприклад, Голосіївський парк, Ботанічний сад ім.акад. О.В. Фоміна) забезпечують менш напружені умови росту і розвитку рослин навіть при відміченому в квітні 2020 р. «дуже високому» рівні забруднення повітря (ІЗА вище 14,0) на цих територіях. Дослідження чутливості пилку F. suspenseдо умов вирощування буде продовжене,оскільки виявлена перспективність використання цього виду для біоіндикації екологічного стану та зонування паркових екосистем Києва за градієнтом антропогенного впливу.Ключові слова: форзиція, фертильність, індекс стерильності, біоіндикація.

scholarly journals SPECIES IDENTIFICATION FOR AQUATIC BIOMONITORING USING DEEP RESIDUAL CNN AND TRANSFER LEARNING

2021 ◽  
Vol 20 (1) ◽  
pp. 001
Author(s):  
Aleksandar Milosavljević ◽  
Đurađ Milošević ◽  
Bratislav Predić
Keyword(s):  
Species Identification ◽  
Transfer Learning ◽  
Aquatic Insects ◽  
Aquatic Ecosystems ◽  
Data Augmentation ◽  
Ecological Status ◽  
Multiple Images ◽  
Public Datasets ◽  
Learning Data ◽  
Small Dataset

Aquatic insects and other benthic macroinvertebrates are mostly used as bioindicators of the ecological status of freshwaters. However, an expensive and time-consuming process of species identification represents one of the key obstacles for reliable biomonitoring of aquatic ecosystems. In this paper, we proposed a deep learning (DL) based method for species identification that we evaluated on several available public datasets (FIN-Benthic, STONEFLY9, and EPT29) along with our Chironomidae dataset (CHIRO10). The proposed method relies on three DL techniques used to improve robustness when training is done on a relatively small dataset: transfer learning, data augmentation, and feature dropout. We applied transfer learning by employing ResNet-50 deep convolutional neural network (CNN) pretrained on ImageNet 2012 dataset. The results show significant improvement compared to original contributions and confirms that there is a considerable gain when there are multiple images per specimen.

scholarly journals The Fellowship of the Ring Test: DNAqua-Net WG2 initiative to compare diatom metabarcoding protocols used in routine freshwater biomonitoring for standardisation

2021 ◽  
Vol 4 ◽  
Author(s):  
Valentin Vasselon ◽  
Éva Ács ◽  
Salomé Almeida ◽  
Karl Andree ◽  
Laure Apothéloz-Perret-Gentil ◽  
...  
Keyword(s):  
Dna Extraction ◽  
High Throughput ◽  
Aquatic Ecosystems ◽  
Quality Index ◽  
Ecological Status ◽  
Pcr Amplification ◽  
Ring Test ◽  
Dna Metabarcoding ◽  
Ecological Status Assessment ◽  
Status Assessment

During the past decade genetic approaches have been developed to monitor biodiversity in aquatic ecosystems. These enable access to taxonomic and genetic information from biological communities using DNA from environmental samples (e.g. water, biofilm, soil) and methods based on high-throughput sequencing technologies, such as DNA metabarcoding. Within the context of the Water Framework Directive (WFD), such approaches could be applied to assess Biological Quality Elements (BQE). These are used as indicators of the ecological status of aquatic ecosystems as part of national monitoring programs of the european network of 110,000 surface water monitoring sites with 79.5% rivers and 11% lake sites (Charles et al. 2020). A high-throughput method has the potential to increase our spatio-temporal monitoring capacity and to accelerate the transfer of information to water managers with the aim to increase protection of aquatic ecosystems. Good progress has been made with developing DNA metabarcoding approaches for benthic diatom assemblages. Technological innovation and protocol optimization have allowed robust taxonomic (species) and genetic (OTU, ESV) information to be obtained from which diatom quality indices can be calculated to infer ecological status to rivers and lakes. Diatom DNA metabarcoding has been successfully applied for biomonitoring at the scale of national river monitoring networks in several countries around the world and can now be considered technically ready for routine application (e.g. Apothéloz-Perret-Gentil et al. 2017, Bailet et al. 2019, Mortágua et al. 2019, Vasselon et al. 2019, Kelly et al. 2020, Pérez-Burillo et al. 2020, Pissaridou et al. 2021). However, protocols and methods used by each laboratory still vary between and within countries, limiting their operational transferability and the ability to compare results. Thus, routine use of DNA metabarcoding for diatom biomonitoring requires standardization of all steps of the metabarcoding procedure, from the sampling to the final ecological status assessment in order to define good practices and standards. Following previous initiatives which resulted in a CEN technical report for biofilm sampling and preservation (CEN 2018), a set of experiments was initiated during the DNAqua-Net WG2 diatom workshop (Cyprus, 2019) to focus on DNA extraction and PCR amplification steps in order to evaluate: i) the transferability and reproducibility of a protocol between different laboratories; ii) the variability introduced by different protocols currently applied by the scientific community. 19 participants from 14 countries performed DNA extraction and PCR amplification in parallel, using i) the same fixed protocol and ii) their own protocol. Experiments were performed by each participant on a set of standardized DNA and biofilm samples (river, lake, mock community). In order to specifically test the variability of DNA extraction and PCR amplification steps, all other steps of the metabarcoding process were fixed and the preparation of the Miseq sequencing was performed by only one laboratory. The variability within and between participants will be evaluated on DNA extracts quantity, taxonomic (genus, species) and genetic richness, community structure comparison and diatom quality index scores (IPS). We will also evaluate the variability introduced by different DNA extraction and PCR amplification protocols on diatom quality index scores and the final ecological status assessment. The results from this collaborative work will not serve to define “one protocol to rule them all”, but will provide valuable information to define guidelines and minimum requirements that should be considered when performing diatom metabarcoding for biomonitoring.

Biological monitoring and assessment of rivers as a basis for identifying and prioritising river management options

1999 ◽  
Vol 39 (10-11) ◽  
pp. 207-210 ◽  
Author(s):  
D. J. Roux ◽  
C. J. Kleynhans ◽  
C. Thirion
Keyword(s):  
Biological Monitoring ◽  
Aquatic Ecosystem ◽  
Aquatic Ecosystems ◽  
River Management ◽  
Management Options ◽  
Specific Knowledge ◽  
Management Objectives ◽  
Ecosystem Condition ◽  
Systematic Framework ◽  
Quantitative Assessments

The output of monitoring programmes such as the National Aquatic Ecosystem Biomonitoring Programme must be used in the management of aquatic ecosystems. To achieve this, collected data must, through a systematic framework, be linked to measurable management objectives. This paper demonstrates how the results obtained with biological indices and system-specific knowledge, are combined to derive semi-quantitative assessments of ecosystem condition. These assessments provide the basis for responding to the results of a river monitoring programme.

scholarly journals Comparison of Methods to Estimate Lake-Surface-Water Temperature Using Landsat 7 ETM+ and MODIS Imagery: Case Study of a Large Shallow Subtropical Lake in Southern Brazil

Water ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 168 ◽  
Author(s):  
Matheus Tavares ◽  
Augusto Cunha ◽  
David Motta-Marques ◽  
Anderson Ruhoff ◽  
J. Cavalcanti ◽  
...  
Keyword(s):  
Surface Water ◽  
Water Temperature ◽  
Single Channel ◽  
Atmospheric Correction ◽  
Ecological Status ◽  
Surface Water Temperature ◽  
Lake Surface ◽  
Subtropical Lake ◽  
Landsat 7 ◽  
The Impact

Water temperature regulates many processes in lakes; therefore, evaluating it is essential to understand its ecological status and functioning, and to comprehend the impact of climate change. Although few studies assessed the accuracy of individual sensors in estimating lake-surface-water temperature (LSWT), comparative analysis considering different sensors is still needed. This study evaluated the performance of two thermal sensors, MODIS and Landsat 7 ETM+, and used Landsat methods to estimate the SWT of a large subtropical lake. MODIS products MOD11 LST and MOD28 SST were used for comparison. For the Landsat images, the radiative transfer equation (RTE), using NASA’s Atmospheric Correction Parameter Calculator (AtmCorr) parameters, was compared with the single-channel algorithm in different approaches. Our results showed that MOD11 obtained the highest accuracy (RMSE of 1.05 ° C), and is the recommended product for LSWT studies. For Landsat-derived SWT, AtmCorr obtained the highest accuracy (RMSE of 1.07 ° C) and is the recommended method for small lakes. Sensitivity analysis showed that Landsat-derived LSWT using the RTE is very sensitive to atmospheric parameters and emissivity. A discussion of the main error sources was conducted. We recommend that similar tests be applied for Landsat imagery on different lakes, further studies on algorithms to correct the cool-skin effect in inland waters, and tests of different emissivity values to verify if it can compensate for this effect, in an effort to improve the accuracy of these estimates.

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