In Fluent, the 3-D RNG k–ε mathematical model is employed to compute water and air mixture pipe flow. The dissolved oxygen convectionaεnd diffusion model is established to simulate the concentration distribution of dissolved oxygen with user defined scalar method. Velocity, pressure and dissolved oxygen concentration are computed. Then, dissolved oxygen concentration and pressure are compared with the data of physical model, and they agree with each other approximately, showing it is valid and reliable to compute the mixture pipe flow and dissolved oxygen concentration with the model .Furthermore, under a specific condition, velocity, pressure and dissolved oxygen concentration of water and air mixture pipe flow are computed and their characteristics are analyzed.