scholarly journals Effect of influent ammonia nitrogen concentration on microbial community in MBBR reactor

Author(s):  
Huizhou Yuan ◽  
Yong Li ◽  
Kun Wang

Abstract The purpose of this study is to explore the nitrogen removal efficiency of the moving bed biofilm reactor (MBBR) under different ammonia nitrogen concentrations (states P1–P5), especially the composition of various forms of nitrogen, related genes and microbial community structure and succession law in the effluent of the reactor. The results show that the average efficiency of MBBR effluent denitrification is 63.63%. The concentration dynamics of NO3−-N and NO2−-N in the effluent indicated a relatively short-range nitrification and denitrification reaction in the MBBR. The results of 16SrDNA sequencing of P1–P5 microbial samples found that changes in the concentration of ammonia nitrogen in the influent produced significant changes in the composition of the microbial community in the MBBR. The genera Ottowia and Flavobacterium played an important role in the nitrogen removal of the MBBR system.

2017 ◽  
Vol 245 ◽  
pp. 1282-1285 ◽  
Author(s):  
Xinbo Zhang ◽  
Zi Song ◽  
Wenshan Guo ◽  
Yanmin Lu ◽  
Li Qi ◽  
...  

2012 ◽  
Vol 23 (5) ◽  
pp. 739-749 ◽  
Author(s):  
Ivar Zekker ◽  
Kristel Kroon ◽  
Ergo Rikmann ◽  
Toomas Tenno ◽  
Martin Tomingas ◽  
...  

2009 ◽  
Vol 2009 (15) ◽  
pp. 2266-2279
Author(s):  
Arbina Shrestha ◽  
Rumana Riffat ◽  
Charles Bott ◽  
Imre Takacs ◽  
Beverley Stinson ◽  
...  

2016 ◽  
Vol 74 (12) ◽  
pp. 2909-2916 ◽  
Author(s):  
Lukasz Kopec ◽  
Jakub Drewnowski ◽  
Adam Kopec

The paper presents research of a prototype moving bed biofilm reactor (MBBR). The device was used for the post-denitrification process and was installed at the end of a technological system consisting of a septic tank and two trickling filters. The concentrations of suspended biomass and biomass attached on the EvU Perl moving bed surface were determined. The impact of the external organic carbon concentration on the denitrification rate and efficiency of total nitrogen removal was also examined. The study showed that the greater part of the biomass was in the suspended form and only 6% of the total biomass was attached to the surface of the moving bed. Abrasion forces between carriers of the moving bed caused the fast stripping of attached microorganisms and formation of flocs. Thanks to immobilization of a small amount of biomass, the MBBR was less prone to leaching of the biomass and the occurrence of scum and swelling sludge. It was revealed that the maximum rate of denitrification was an average of 0.73 gN-NO3/gDM·d (DM: dry matter), and was achieved when the reactor was maintained in external organic carbon concentration exceeding 300 mgO2/dm3 chemical oxygen demand. The reactor proved to be an effective device enabling the increase of total nitrogen removal from 53.5% to 86.0%.


2007 ◽  
Vol 2007 ◽  
pp. 214-214
Author(s):  
Alireza Vakili ◽  
Mohsen Danesh Mesgaran ◽  
Reza Valizadeh ◽  
Alireza Heravi Moussavi ◽  
Mohammad Reza Nassiry ◽  
...  

In ruminants, as much as 50% of the dietary crude protein can be converted to ammonia by ruminal microorganisms. A part of ammonia can be utilized as a bacterial nitrogen source; however, rates of ammonia production often exceed rates of ammonia utilization. Peptides are intermediates in the conversion of ingested protein to ammonia in the rumen and their accumulation depends upon the nature of diet (Mesgaran & Parker, 1995). The objective of the present experiment was to investigate the effect of diets differing in concentrate: lucerne hay ratios on the ruminal pH, ammonia-nitrogen concentration and ruminal peptide nitrogen concentration in Holstein steers.


2012 ◽  
Vol 518-523 ◽  
pp. 214-217
Author(s):  
Tao Hong Liao ◽  
Kang Huai Liu ◽  
Jia Chun Yang ◽  
Kenji Furukawa

This experiment aimed to explore the effect of high- concentration nitrogen removal by Anaerobic ammonium oxidation process. The mixed liquor suspended solid(MLSS)was 510 mg/L. In the beginning of the experiment, the inflow concentration rate of ammonia nitrogen and nitrite nitrogen was about 1:1.3 and the total nitrogen concentration was low. When the reactor reached the ideal treatment effect then gradually increased the total nitrogen concentration, until reaching the maximum nitrogen load rate(NLR) of the reactor. In the temperature of 34.4 °C, PH was 7.23, the inflow concentration of ammonia nitrogen was 223.3 mg. N/L, the inflow concentration nitrite nitrogen was 289.7 mg. N/L, the dissolved oxygen (DO) was 2.3 mg. N/L, the nitrogen load rate (NLR) was 6.08 kg. N / (m3. d), the nitrogen removal rate (NRR) was 5.60 kg. N / (m3. d), the total nitrogen (TN) removal rate was 92.2%, under this conditions, the reactor achieved the best effect.


Water ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 3240
Author(s):  
Jinfeng Jiang ◽  
Liang Ma ◽  
Lianjie Hao ◽  
Daoji Wu ◽  
Kai Wang

In order to achieve advanced nitrogen removal from landfill leachate without the addition of external carbon sources, a Sequencing Batch Reactor (SBR) and a Sequencing Biofilm Batch Reactor (SBBR) were proposed for the treatment of actual landfill leachate with ammonia nitrogen (NH4+-N) and chemical oxygen demand (COD) concentrations of 1000 ± 100 mg/L and 4000 ± 100 mg/L, respectively. The operating modes of both systems are anaerobic–aerobic–anoxic. After 110 days of start-up and biomass acclimation, the effluent COD and the total nitrogen (TN) of the two systems were 650 ± 50 mg/L and 20 ± 10 mg/L, respectively. The removal rates of COD and total nitrogen could reach around 85% and above 95%, respectively. Therefore, advanced nitrogen removal was implemented in landfill leachate without adding any carbon sources. After the two systems were acclimated, nitrogen removing cycles of SBR and SBBR were 24 h and 20 h, respectively. The nitrogen removing efficiency of SBBR was improved by 16.7% in comparison to SBR. In the typical cycle of the two groups of reactors, the nitrification time of the system was the same, which was 5.5 h, indicating that although the fiber filler occupied part of the reactor space, it had no significant impact on the nitrification performance of the system. At the end of aeration, the internal carbon source content of sludge of SBBR was equivalent to that of the SBR system. However, the total nitrogen concentration of SBBR was only 129 mg/L, which is 33.8% lower than that of SBR at 195 mg/L. The main reason was that biofilm enhanced the simultaneous nitrification and denitrification (SND) effect of the system.


Water ◽  
2021 ◽  
Vol 13 (18) ◽  
pp. 2484
Author(s):  
César Huiliñir ◽  
Vivian Fuentes ◽  
Carolina Estuardo ◽  
Christian Antileo ◽  
Ernesto Pino-Cortés

This work aimed to achieve partial nitrification (PN) in a Sequencing Moving Bed Biofilm Reactor SMBBR with zeolite as a biomass carrier by using sulfide pulses in the presence of organic matter as an inhibitor. Two conditions were evaluated: sulfide (HS−) = 5 mg S/L and vvm (air volume per liquid volume per minute, L of air L−1 of liquid min−1) = 0.1 (condition 1); and a HS− = 10 mg S/L and a vvm = 0.5 (condition 2). The simultaneous effect of organic matter and sulfide was evaluated at a Chemical Oxygen Demand (COD) = 350 mg/L and HS− = 5 mg S/L, with a vvm = 0.5. As a result, using the sulfide pulse improved the nitrite accumulation in both systems. However, Total Ammonia Nitrogen (TAN) oxidation in both processes decreased by up to 60%. The simultaneous presence of COD and sulfide significantly reduced the TAN and nitrite oxidation, with a COD removal yield of 80% and sulfide oxidation close to 20%. Thus, the use of a sulfide pulse enabled PN in a SMBBR with zeolite. Organic matter, together with the sulfide pulse, almost completely inhibited the nitrification process despite using zeolite.


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