Nutrient removal from pickle industry wastewater by cultivation of Chlorella pyrenoidosa for lipid production

2019 ◽  
Vol 79 (11) ◽  
pp. 2166-2174 ◽  
Author(s):  
Liang Wan ◽  
Yixiao Wu ◽  
Xuemei Zhang ◽  
Weihao Zhang
Keyword(s):  
Nutrient Removal ◽  
Methyl Esters ◽  
Lipid Production ◽  
Oxygen Demand ◽  
Biomass Concentration ◽  
Biomass Productivity ◽  
Chlorella Pyrenoidosa ◽  
Algae Biomass ◽  
Nutrient Removal Efficiency ◽  
Industry Wastewater

AbstractThe present research examined the feasibility of cultivating Chlorella pyrenoidosa in pickle industry wastewater for simultaneous nutrient removal and lipid production. The characteristics of microalgae growth, nutrient removal, lipid accumulation and composition of C. pyrenoidosa cultivated in pickle wastewater with different dilution ratios were investigated. The results showed the maximum algae biomass concentration of 1.57 ± 0.12 g L−1 was achieved in non-diluted pickle wastewater with the highest biomass productivity of 170.65 mg L−1 day−1. Maximum nutrient removal efficiency was observed in 20.0% pickle wastewater with removal rates of chemical oxygen demand (COD), total phosphorus (TP), total nitrogen (TN) and NH4-N at 84.67%, 92.46%, 85.82% and 93.42%, respectively. The lipid content of C. pyrenoidosa growing in pickle wastewater ranged from 29.73% to 31.78%, with a highest lipid productivity of 57.23 mg L−1 day−1. The relative content of triolefinic acids (C16:3 and C18:3) decreased while the monoenoic acids (C16:1 and C18:1) increased synchronously with the pickle wastewater concentration. Unsaturated fatty acid methyl esters were the main components, ranging from 73.04% to 77.6%. The biodiesel properties satisfied the major specifications in US and European biodiesel standards. The results indicated that C. pyrenoidosa is a promising species for nutrient removal together with lipid production in pickle industry wastewater.

scholarly journals Assessment of Domestic Wastewaters as Potential Growth Media for Chlorella vulgaris and Haematococcus pluvialis

2022 ◽  
Vol 30 (1) ◽  
pp. 565-580
Author(s):  
Yeong Hwang Tan ◽  
Mee Kin Chai ◽  
Yang Kai Ooi ◽  
Ling Shing Wong
Keyword(s):  
Removal Efficiency ◽  
Chlorella Vulgaris ◽  
Nutrient Removal ◽  
Haematococcus Pluvialis ◽  
Biomass Concentration ◽  
Basal Medium ◽  
Domestic Wastewater ◽  
Chemical Compounds ◽  
Growth Media ◽  
Nutrient Removal Efficiency

Domestic wastewater contains chemical compounds that can be used as nutrients for microalgae. Removing these chemical compounds from wastewater by microalgae might help in reducing the operation cost of wastewater management while minimizing the cultivation cost for large-scale microalgae metabolite production. In this study, domestic wastewater collected from Indah Water Konsortium (IWK), Kuala Lumpur, Malaysia, was assessed as growth media for two types of microalgae, namely Chlorella vulgaris and Haematococcus pluvialis. The biomass growth and nutrient removal efficiency of total nitrogen (TN), total phosphorus (TP), and total ammonia (TAN) in different concentrations of diluted wastewater were measured. The results showed that biomass concentration (0.227 g/L), biomass productivity (0.029 g/L/day), and specific growth rate (0,284 d-1) yielded by C. vulgaris in 14 days of 80% wastewater were comparable to those microalgae grew in standard Bold’s Basal medium (BBM). Besides, C. vulgaris grew in 50% wastewater to remove TN, TP, and TAN with the highest removal efficiency (>88%). For H. pluvialis, the biomass concentration in all wastewater concentrations was lower than BBM. The removal efficiencies of TN and TP were lower than 55%, but more than 80% for removal efficiency of TAN in 50% and 80% wastewater. Hence, C. vulgaris has better growth performance and nutrient removal efficiency than H. pluvialis. These findings indicated that IWK domestic wastewater could be used as growth media for microalgae, especially C. vulgaris.

scholarly journals Biomass and Lipid Production Potential of an Indian Marine Algal Isolate Tetraselmis striata BBRR1

Energies ◽  
2020 ◽  
Vol 13 (2) ◽  
pp. 341 ◽  
Author(s):  
Annakkili Baskara Boopathy ◽  
Thanasekaran Jayakumar ◽  
Senthil Chinnasamy ◽  
Muthu Ganesan Rajaram ◽  
Natarajan Mohan ◽  
...  
Keyword(s):  
Tamil Nadu ◽  
Lipid Production ◽  
Octadecenoic Acid ◽  
Biomass Concentration ◽  
Biomass Productivity ◽  
Systematic Position ◽  
Tetraselmis Chuii ◽  
Tetraselmis Striata ◽  
Different Strains ◽  
Raceway Ponds

Four different strains of marine algae viz. Tetraselmis tetrathele, Tetraselmis striata, Tetraselmis chuii, and Tetraselmis gracilis were isolated from the saltpans in Kovelong, Chennai, Tamil Nadu, India. The systematic position of Tetraselmis striata BBRR1 was confirmed through molecular identification. Under laboratory conditions, T. striata Butcher BBRR1 grown in f/2-medium recorded highest biomass concentration of 0.58 ± 0.021 g L−1, volumetric productivity of 0.025 ± 0.004 g L−1 d−1, 19 ± 2.3% proteins, 17 ± 1.5% carbohydrates, and 15 ± 2.4% lipids. Volumetric biomass productivity of 0.063 ± 0.08 g L−1 d−1, specific growth rate of 0.45 day−1 and lipid content of 19.42 ± 0.98% were recorded for the alga T. striata Butcher BBRR1 cultivated in 10-m2 open raceway ponds using Modified CFTRI ABRR1 medium. The fatty acid profile of T. striata Butcher BBRR1 showed the presence of 33.14% palmitic acid, 22.64% 11-octadecenoic acid, and 21.94% heptadecanoic acid. This study confirms the feasibility of cultivating the marine alga T. striata in open raceway ponds to produce biomass, which can be used for the production of biofuels.

Using wastewater and high-rate algal ponds for nutrient removal and the production of bioenergy and biofuels

2013 ◽  
Vol 67 (4) ◽  
pp. 915-924 ◽  
Author(s):  
David Batten ◽  
Tom Beer ◽  
George Freischmidt ◽  
Tim Grant ◽  
Kurt Liffman ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Nutrient Removal ◽  
Large Scale ◽  
Oxygen Demand ◽  
Recent Change ◽  
High Rate ◽  
Coastal Zones ◽  
Nitrogen And Phosphorus ◽  
Treatment Technology ◽  
Algae Biomass

This paper projects a positive outcome for large-scale algal biofuel and energy production when wastewater treatment is the primary goal. Such a view arises partly from a recent change in emphasis in wastewater treatment technology, from simply oxidising the organic matter in the waste (i.e. removing the biological oxygen demand) to removing the nutrients – specifically nitrogen and phosphorus – which are the root cause of eutrophication of inland waterways and coastal zones. A growing need for nutrient removal greatly improves the prospects for using new algal ponds in wastewater treatment, since microalgae are particularly efficient in capturing and removing such nutrients. Using a spreadsheet model, four scenarios combining algae biomass production with the making of biodiesel, biogas and other products were assessed for two of Australia’s largest wastewater treatment plants. The results showed that super critical water reactors and anaerobic digesters could be attractive pathway options, the latter providing significant savings in greenhouse gas emissions. Combining anaerobic digestion with oil extraction and the internal economies derived from cheap land and recycling of water and nutrients on-site could allow algal oil to be produced for less than US$1 per litre.

scholarly journals Treatment and Re-Use of Raw Blackwater by Chlorella vulgaris-Based System

Water ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2660
Author(s):  
Marco Antonio Segovia Bifarini ◽  
Miha Žitnik ◽  
Tjaša Griessler Bulc ◽  
Aleksandra Krivograd Klemenčič
Keyword(s):  
Biomass Production ◽  
Chlorella Vulgaris ◽  
Nutrient Removal ◽  
Liquid Waste ◽  
Oxygen Demand ◽  
Biomass Productivity ◽  
Dry Weight ◽  
Nutrient Content ◽  
Total Biomass ◽  
Sanitation Systems

In this study, we examined a Chlorella vulgaris-based system as a potential solution to change liquid waste, such as blackwater, into valuable products for agriculture while protecting waters from pollution without technical demanding pre-treatment. To evaluate the possibility of nutrient removal and biomass production from raw blackwater, four blackwater dilutions were tested at lab-scale: 50%, 30%, 20%, and 10%. The results showed that even the less diluted raw blackwater was a suitable growth medium for microalgae C. vulgaris. As expected, the optimum conditions were observed in 10% blackwater with the highest growth rate (0.265 d−1) and a nutrient removal efficiency of 99.6% for ammonium and 33.7% for phosphate. However, the highest biomass productivity (5.581 mg chlorophyll-a L−1 d−1) and total biomass (332.82 mg dry weight L−1) were achieved in 50% blackwater together with the highest chemical oxygen demand removal (81%) as a result of the highest nutrient content and thus prolonged growth phase. The results suggested that the dilution factor of 0.5 followed by microalgae cultivation with a hydraulic retention time of 14 days could offer the highest biomass production for the potential use in agriculture and, in parallel, a way to treat raw blackwater from source-separation sanitation systems.

scholarly journals Peningkatan Phycocyanin pada Spirulina Platensis dengan Media Limbah Virgin Coconut Oil pada Photobioreactor Tertutup

Eksergi ◽  
2016 ◽  
Vol 13 (2) ◽  
pp. 1
Author(s):  
Sri Sukadarti ◽  
Sri Wahyu Murni ◽  
M.M. Azimatun Nur
Keyword(s):  
Light Intensity ◽  
Spirulina Platensis ◽  
Oxygen Demand ◽  
Biomass Concentration ◽  
Coconut Oil ◽  
Biomass Productivity ◽  
Virgin Coconut Oil ◽  
Blue Green Algae ◽  
Anti Oxidant ◽  
Excellent Source

Waste of Virgin Coconut Oil (VCO) industries has a high value of Chemical Oxygen Demand (COD), it is therefore a problem for the environment. This waste contains organic materials such as oil, proteins, carbohydrates and some minerals, that potentially to be used for the cultivation of blue-green algae Spirulina plantesis.  Spirulina plantesis is an excellent source of phycocyanin. The Phycocyanin has anti-aging, anti-oxidant and anti-inflammatory properties, it is therefore considered useful. This research is aimed to study the effects of the addition of urea and light intensity on the growth of Spirulina, the concentration of phycocyanin and decreasing of COD value. Spirulina platensis was cultivated in a closed photobioreactor with an air flow for 7 days.  The light intensity was varied as follows 5000 lux, 6000 lux, 7000 lux and 8000 lux, and the addition of urea as nutrients was also varied as follows 40 ppm, 50 ppm, 60 ppm and 70 ppm.  This research indicated that the optimum condition was obtained at the addition of urea of 70 ppm, light intensity  of 6000 lux. This research resulted μmax of 1,1375day-1 , the biomass  productivity of 0,0423 g/l/day,  biomass concentration of   0,1734 g/l  and  phycocyanin concentration of 5,047%. The largest of COD removal is was 98,06% and the COD value of  280 mg/l was finally achieved.

Optimization of simultaneous biomass production and nutrient removal by mixotrophic Chlorella sp. using response surface methodology

2015 ◽  
Vol 73 (7) ◽  
pp. 1520-1531 ◽  
Author(s):  
Yu-Ru Lee ◽  
Jen-Jeng Chen
Keyword(s):  
Removal Efficiency ◽  
Biomass Production ◽  
Nutrient Removal ◽  
Biomass Productivity ◽  
Aeration Rate ◽  
Effect Of Temperature ◽  
Piggery Wastewater ◽  
Cultivation Time ◽  
Chlorella Sp ◽  
Nutrient Removal Efficiency

The bioprospecting of potentially mixotrophic microalgae in a constructed wetland was conducted. A locally isolated microalga, Chlorella sp., was grown to determine the effect of temperature, aeration rate, and cultivation time on simultaneous biomass production and nutrient removal from piggery wastewater using central composite design (CCD). The most important variable for the biomass productivity of Chlorella sp. was aeration rate, while that for lipid content and nutrient removal efficiency was cultivation time. Total nitrogen (TN) and total phosphorus (TP) removal efficiencies were higher than that of chemical oxygen demand (COD) from piggery wastewater. The CCD results indicate that the highest biomass productivity (79.2 mg L−1 d−1) and simultaneous nutrient removal efficiency (TN 80.9%, TP 99.2%, COD 74.5%) were obtained with a cultivation temperature of 25 °C, a cultivation time of 5 days, and an air aeration rate of 1.6 L L−1 min−1. Palmitic acid (C16:0) and linoleic acid (C18:2) were both abundant in Chlorella sp. cells under mixotrophic cultivation with piggery wastewater.

scholarly journals Algal Remediation of Wastewater Produced from Hydrothermally Treated Septage

2019 ◽  
Vol 11 (12) ◽  
pp. 3454 ◽  
Author(s):  
Kyle McGaughy ◽  
Ahmad Abu Hajer ◽  
Edward Drabold ◽  
David Bayless ◽  
M. Toufiq Reza
Keyword(s):  
Wastewater Treatment ◽  
Treatment Plant ◽  
Oxygen Demand ◽  
Biomass Concentration ◽  
Hydrothermal Carbonization ◽  
Septic Tank ◽  
Algae Biomass ◽  
Untreated Wastewater ◽  
Chlorella Sp ◽  
Short Period

Hydrothermal carbonization (HTC) is a promising technology to convert wet wastes like septic tank wastes, or septage, to valuable platform chemical, fuels, and materials. However, the byproduct of HTC, process liquid, often contains large amount of nitrogen species (up to 2 g/L of nitrogen), phosphorus, and a variety of organic carbon containing compounds. Therefore, the HTC process liquid is not often treated at wastewater treatment plant. In this study, HTC process liquid was treated with algae as an alternative to commercial wastewater treatment. The HTC process liquid was first diluted and then used to grow Chlorella sp. over a short period of time (15 days). It was found that the algae biomass concentration increased by 644 mg/L over the course of 10 days, and which subsequently removed a majority of the nutrients in the HTC process liquid. Around 600 mg/L of algal biomass was collected in the process liquid at the end of treatment (day 15). Meanwhile, chemical oxygen demand (COD), total phosphorous, total Kheldjal nitrogen, and ammonia were reduced by 70.0, 77.7, 82.2, and 99.0% by fifteen days compared to the untreated wastewater, respectively. This study demonstrates that HTC process liquid can be treated by growing algae creating a potential replacement for expensive synthetic nutrient feeds for algal production.

Sign in / Sign up

Export Citation Format

Share Document