Microbial ecological processes in MBBR biofilms for biological phosphorus removal from wastewater

2019 ◽  
Vol 79 (8) ◽  
pp. 1467-1473 ◽  
Author(s):  
Knut Rudi ◽  
Inger Andrea Goa ◽  
Torgeir Saltnes ◽  
Gjermund Sørensen ◽  
Inga Leena Angell ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Phosphorus Removal ◽  
Alpha Diversity ◽  
Fold Increase ◽  
Environmental Pollutant ◽  
Biofilm Reactor ◽  
Operational Performance ◽  
Biological Phosphorus Removal ◽  
Ecological Processes ◽  
Biological Phosphorus

Abstract Phosphorus is both a major environmental pollutant and a limiting resource. Although enhanced biological phosphorus removal (EBPR) is used worldwide for phosphorus removal, the standard activated sludge-based EBPR process shows limitations with stability and efficiency. Recently, a new EBPR moving bed biofilm reactor (MBBR) process has been developed at HIAS (Hamar, Norway), enabling a phosphorus removal stability above 90% during a whole year cycle. To increase the knowledge of the HIAS (MBBR) process the aim of the current work was to characterize the MBBR microbiota and operational performance weekly for the operational year. Surprisingly, we found a major succession of the microbiota, with a five-fold increase in phosphorus accumulating organisms (PAOs), and major shifts in eukaryote composition, despite a stable phosphorus removal. Temperature was the only factor that significantly affected both phosphorus removal and the microbiota. There was a lower phosphor removal during the winter, coinciding with a higher microbiota alpha diversity, and a lower beta diversity. This differs from what is observed for activated sludge based EBPR. Taken together, the knowledge gained from the current microbiota study supports the efficiency and stability of MBBR-based systems, and that knowledge from activated sludge-based EBPR approaches cannot be translated to MBBR systems.

Biological Phosphorus Removal in a Sequencing Batch Moving Bed Biofilm Reactor

1999 ◽  
Vol 40 (4-5) ◽  
pp. 161-168 ◽  
Author(s):  
H. Helness ◽  
H. Ødegaard
Keyword(s):  
Phosphorus Removal ◽  
Batch Reactor ◽  
Complete Removal ◽  
Biofilm Reactor ◽  
Biological Phosphorus Removal ◽  
Moving Bed Biofilm Reactor ◽  
Moving Bed ◽  
Biofilm Carrier ◽  
Biofilm Process ◽  
Biological Phosphorus

Experiments have been carried out with biological phosphorus removal in a sequencing batch moving bed biofilm reactor (SBMBBR) with a plastic biofilm carrier (Kaldnes) suspended in the wastewater. The aim of the research leading to this paper was to evaluate biological phosphorus removal in this type of biofilm process. Biological phosphorus removal can be achieved in a moving bed biofilm reactor operated as a sequencing batch reactor. In order to achieve good and stable phosphorus removal over time, the length of the anaerobic period should be tuned to achieve near complete removal of easily biodegradable COD in the anaerobic period. The total COD-loading rate must at the same time be kept high enough to achieve a net growth of biomass in the reactor. Use of multivariate models based on UV-absorption spectra and measurements of the redox potential show potential for control of such a process.

Innovative methods for sludge characterization in biological phosphorus removal systems

1999 ◽  
Vol 39 (6) ◽  
pp. 37-43 ◽  
Author(s):  
Damir Brdjanovic ◽  
Mark C. M. van Loosdrecht ◽  
Christine M. Hooijmans ◽  
Takashi Mino ◽  
Guy J. Alaerts ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Phosphorus Removal ◽  
Chemical Measurement ◽  
Biological Phosphorus Removal ◽  
Ongoing Research ◽  
Innovative Methods ◽  
Sludge Characterization ◽  
Biological Phosphorus

This paper describes several techniques and methods for sludge characterization which are recently developed and used in our ongoing research on biological phosphorus removal (BPR): (i) chemical measurement of the poly-hydroxy-alkanoate (PHA) content of the biomass, (ii) bioassay for glycogen determination in BPR systems, (iii) determination of the fraction of denitrifying phosphorus accumulating organisms (DPAOs) in P-removing sludge, (iv) determination of the aerobic and anoxic ATP/NADH2 ratio (δ value) in BPR systems, and (v) determination of the glycogen accumulating organisms (GAOs) / phosphorus accumulating organisms (PAOs) ratio in activated sludge.

The Influence of Nitrogen on Phosphorus Removal in Activated Sludge Plants

1982 ◽  
Vol 14 (1-2) ◽  
pp. 31-45 ◽  
Author(s):  
J L Barnard
Keyword(s):  
Organic Carbon ◽  
Activated Sludge ◽  
Phosphorus Removal ◽  
Complete Removal ◽  
Phosphate Removal ◽  
Electron Donors ◽  
Biological Phosphorus Removal ◽  
Anaerobic Conditions ◽  
Low Level ◽  
Biological Phosphorus

This paper discusses the need for anaerobiases as a pre-requisite for phosphorus removal in activated sludge plants and the effect of nitrates on the anaerobic conditions. If the plant could be operated to avoid nitrification, biological phosphorus removal presents no problems. When nitrification is required, the nitrates must be reduced to a low level through internal denitrification. If sufficient carbon is available to ensure complete removal of the nitrates and anaerobic conditions in a specific zone in the plant, good phosphate removal can be ensured. Below COD : TKN ratios of 10 : 1 it is becoming more difficult to control the plant and special care should be taken to determine not only the quantity of organic carbon available as electron donors for removal of the nitrates but also the form in which it arrives at the plant.

Biochemical Aspects of Enhanced Biological Phosphorus Removal from Wastewater

1985 ◽  
Vol 17 (11-12) ◽  
pp. 23-41 ◽  
Author(s):  
M. C. Hascoet ◽  
M. Florentz ◽  
P. Granger
Keyword(s):  
Activated Sludge ◽  
Phosphorus Removal ◽  
Phosphorus Uptake ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
The Past ◽  
Nitrate Addition ◽  
The Subject ◽  
Biological Phosphorus ◽  
Microbiological Analyses

Enhanced biological phosphorus removal from wastewater by means of microorganisms found in activated sludge has for the past few years been the subject of much research and it is now commonly recognized that an activated sludge system must include alternating anaerobic-aerobic periods. The present article covers biochemical aspects of this phenomenon using a phosphorus removing biomass obtained in a laboratory-scale pilot with alternating phases and a synthetic substrate feed. The percentage of phosphorus obtained in the pilot sludge was four times greater than that of a conventional sludge plant. By exposing the same biomass to different conditions and using 31P Nuclear Magnetic Resonance, we were able to accurately pin-point the various forms of phosphorus found within cells and follow their development during the course of alternating phases. The following results were obtained:the transformation of phosphorus in its inorganic to polyphosphate form depends on the medium's level of oxygenation,the presence of nitrates disturbs the anaerobic period but does not affect phosphorus uptake in the aerated period.Continuous nitrate addition alters biomass behaviour in the anaerobic phase, which loses the capacity to release phosphorus,copper at a concentration of over 1 mg Cu2+/1 inhibits phosphorus uptake in the aerated phase. Various microbiological analyses made on the pilot biomass isolated conventional bacteria found in activated sludge.

Design of nutrient removal activated sludge systems

2003 ◽  
Vol 47 (11) ◽  
pp. 115-122 ◽  
Author(s):  
J. Manga ◽  
J. Ferrer ◽  
A. Seco ◽  
F. Garcia-Usach
Keyword(s):  
Mathematical Model ◽  
Activated Sludge ◽  
Nutrient Removal ◽  
Phosphorus Removal ◽  
Pilot Plant ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
User Friendly ◽  
Biological Phosphorus ◽  
Activated Sludge Systems

A mechanistic mathematical model for nutrient and organic matter removal was used to describe the behavior of a nitrification denitrification enhanced biological phosphorus removal (NDEBPR) system. This model was implemented in a user-friendly software DESASS (design and simulation of activated sludge systems). A 484-L pilot plant was operated to verify the model results. The pilot plant was operated for three years over three different sludge ages. The validity of the model was confirmed with data from the pilot plant. Also, the utility of DESASS as a valuable tool for designing NDEBPR systems was confirmed.

Practical Experience with Biological Phosphorus Removal Plants in Johannesburg

1983 ◽  
Vol 15 (3-4) ◽  
pp. 233-259 ◽  
Author(s):  
A R Pitman ◽  
S L V Venter ◽  
H A Nicholls
Keyword(s):  
Activated Sludge ◽  
Nutrient Removal ◽  
Phosphorus Removal ◽  
Operating Experience ◽  
Practical Experience ◽  
Biological Nutrient Removal ◽  
Biological Phosphorus Removal ◽  
Factors Affecting ◽  
Process Improvements ◽  
Biological Phosphorus

This paper describes three years operating experience with two full-scale biological nutrient removal activated sludge plants. Factors affecting biological phosphorus removal are highlighted and possible process improvements suggested.

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