scholarly journals Isolation and identification of an aerobic denitrifying phosphorus removing bacteria and analysis of the factors influencing denitrification and phosphorus removal

2018 ◽  
Vol 78 (11) ◽  
pp. 2288-2296 ◽  
Author(s):  
Hongying Xu ◽  
Ru Jin ◽  
Chan Zhang ◽  
Yupeng Wu ◽  
Xiaohui Wang
Keyword(s):  
Escherichia Coli ◽  
16S Rdna ◽  
Removal Efficiency ◽  
Phosphorus Removal ◽  
Municipal Wastewater ◽  
Screening Method ◽  
Treatment Plant ◽  
Effect Of Temperature ◽  
Nitrogen And Phosphorus ◽  
Isolation And Identification

Abstract Excessive emission of plant nutrients (such as nitrogen and phosphorus) into the water body can induce eutrophication. Therefore, how to control eutrophic water efficiently and economically is very important. In the paper, highly efficient aerobic denitrifying phosphorus removing J16 bacteria was isolated from the activated sludge of an aerobic bioreactor in Taiyuan municipal wastewater treatment plant by using the blue–white spot screening method, an aerobic phosphorus absorption test, nitrate reduction test, nitrogen removal experiments, and plate coating and streaking methods. Through 16S rDNA gene homology comparison and physiological and biochemical identification, the J16 strain was preliminarily identified as Escherichia coli, with a sequence similarity of 99%. The 16S rDNA sequence of strain J16 was submitted to GenBank (accession number: MF667015). The effect of temperature, pH, percentage of inoculum and phosphate-P (PO43−-P) concentration on denitrification and phosphorus removal efficiency was investigated through a single-factor experiment. The optimum conditions of the J16 strain for denitrification and phosphorus removal were as follows: 30°C, neutral or weak alkaline (pH: 7.2–8), and 3% of inoculum, respectively. The denitrification and phosphorus removal efficiency of strain J16 was the highest when PO43−-P and nitrate-N(NO3−-N) concentrations were 8.9 and 69.31 mg/L, and the removal were 96.03% and 94.55%, respectively. In addition, strain J16 could reduce phosphoric acid to phosphine (PH3) and remove some phosphorus under hypoxia conditions. This is the first study to report the involvement of Escherichia coli in nitrogen and phosphorus removal under aerobic and hypoxia conditions. Based on the above results, the strain J16 can effectively remove nitrogen and phosphorus, and will be utilized in enhancing treatment of nitrogen and phosphorus-containing industrial wastewater and phosphorus reclamation.

Application of reversed A2/O process on removing nitrogen and phosphorus from municipal wastewater in China

2011 ◽  
Vol 63 (10) ◽  
pp. 2138-2142 ◽  
Author(s):  
X. S. Kang ◽  
C. Q. Liu ◽  
B. Zhang ◽  
X. J. Bi ◽  
F. Zhang ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Phosphorus Removal ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Practical Application ◽  
Nitrogen And Phosphorus ◽  
Nitrogen And Phosphorus Removal ◽  
New Process ◽  
Technical Features

The application of reversed A2/O process in practice in China is mainly discussed in this paper. As a new process on nitrogen and phosphorus removal, principle and technical features of reversed A2/O process are also summarized. The application in rebuilt wastewater treatment plant shows that reversed A2/O process not only has merits on high nitrogen and phosphorus removal efficiency, but also has merits on energy saving. The application in newly-build wastewater treatment plant shows that infrastructure and equipment investment of reversed A2/O process economized 15% and 10% respectively, compared to conventional A2/O process. The practical application shows that reversed A2/O process is a new nitrogen and phosphorus removal process, which is suitable for China's national conditions.

Research on the Fast Start-up of Anaerobic-Aerobic-Anoxic-Aerobic Sequencing Batch Reactor

2011 ◽  
Vol 374-377 ◽  
pp. 1013-1016
Author(s):  
Hui Yang ◽  
Yu Zhang ◽  
Yue Xu
Keyword(s):  
Phosphorus Removal ◽  
Sequencing Batch Reactor ◽  
Municipal Wastewater ◽  
Removal Rate ◽  
Batch Reactor ◽  
Treatment Plant ◽  
Nitrogen And Phosphorus ◽  
Nitrogen And Phosphorus Removal ◽  
Start Up ◽  
Fast Start

Abstract. The paper aims to study the fast start-up of anaerobic-aerobic-anoxic-aerobic sequencing batch reactor, with domestic sewage as treating object, to solve the problem of SBR that can be used for denitrification or dephosphorization independently and to realize simultaneous nitrogen and phosphorus removal in a single SBR system. Phosphorus accumulating organisms were enriched at the anaerobic condition for 2h/aerobic for 3h after activated sludge were inoculated. Then denitrifying polyphosphate-accumulating organisms were enriched by inserting an anoxic phase into the aerobic phase. The lengths of anaerobic time, anoxic time and aerobic time were adjusted and the nitrogen and phosphorus removal effect of (AO)2SBR system were observed. The (AO)2SBR system was started successfully with 80d of training and domesticating. The nitrogen and phosphorus removal effect was performed preferably at the condition of anaerobic(2h)-aerobic(1.5h)-anoxic(1.5h)-aerobic(0.5h). The removal rate of COD, NH4+-N, TN and TP reached 90%, 97%, 88% and 92% respectively. And 33% of energy was saved when aerobic time was shortened from 3h to 2h, while the treating effect dropped off rarely. The results show that (AO)2SBR is applicable for simultaneous nitrogen and phosphorus removal, and the effluent water quality meets the first level B criteria specified in Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant(GB 18918-2002). The system can also reach the aim of saving energy and providing theoretical basis for the nitrogen and phosphorus removal in single SBR systems.

Nitrogen and phosphorus removal using fluidized-carriers in a full-scale A2O biofilm system

2020 ◽  
Author(s):  
Keke Xiao
Keyword(s):  
Wastewater Treatment ◽  
Phosphorus Removal ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Full Scale ◽  
Anammox Bacteria ◽  
Municipal Wastewater Treatment ◽  
Nitrogen And Phosphorus ◽  
Class Level ◽  
Microbial Analysis

<p>A full-scale biofilm system using fluidized-carriers integrated with anaerobic-anoxic–aerobic process (treatment capacity of 3.75 × 10<sup>5</sup> m<sup>3</sup> d<sup>−1</sup>) was used for municipal wastewater treatment. The results indicated relatively higher removal efficiencies of 86% total nitrogen (TN), 97% ammonium (NH<sub>4</sub>-N) and 97% total phosphorus (TP) were achieved, with 0.32 mg L<sup>−1</sup> TP, 0.81 mg L<sup>−1</sup> NH<sub>4</sub>-N and 8.07 mg L<sup>−1</sup> TN in the effluent, which meet the Class A of Discharge standard of pollutants for municipal wastewater treatment plant (GB18918-2002) of China. The results of microbial analysis indicated that the dominant microorganisms in the suspended sludge were Proteobacteria and Bacteroidetes at phylum level and β-Proteobacter at class level. The dominant microorganism in the biofilm was Proteobacteria at phylum level, with γ-Proteobacter (17.5%), β-Proteobacter (14%) and δ-Proteobacter (13.08%) distributed at class levels. The presence of Proteobacteria and Bacteroidetes in this system may be related with the phosphorus removal. A reddish color biofilm was formed on the surface of fluidized-carriers in the anaerobic tank and showed specific anammox ability, this may be related with the dominance of 0.0278% Planctomycetaceae at family level and 0.0278% Planctomycetales at order level. Besides the denitrification effects, the possible anammox bacteria present in the anaerobic tank might have also contributed to high nitrogen removal efficiency.</p>

Study on the Sewage Treatment by SBBR-BAF Filled with Ceramic Filling

2011 ◽  
Vol 299-300 ◽  
pp. 3-6
Author(s):  
Wei Li ◽  
Wei Wei ◽  
Yuan Liu ◽  
Tian Jiao Wang
Keyword(s):  
Removal Efficiency ◽  
Municipal Wastewater ◽  
Sewage Treatment ◽  
Treatment Plant ◽  
Biological Aerated Filter ◽  
Municipal Wastewater Treatment ◽  
Nitrogen And Phosphorus ◽  
Save Energy ◽  
Aerated Filter ◽  
Aeration Volume

In the biological aerated filter, the removal efficiency of phosphorus is poor, while the efficiency of organics and nitrogen are very well. In order to solve these problems, the sequence bio-film reactor connects to traditional biological aerated filter, ceramic filler was added to the system, study the effect of bio-film culturing and biological disposal capacity .Control intermittent time of anaerobic and aerobic and aeration volume, the removal effect of COD 、nitrogen and phosphorus are good in the sewage . The results show that anaerobic and aerated time are 3h and 4h in SBBR, the removal efficiency of COD、NH4+-N、TN and TP are 90.07%、85.12%、81.74% and 83.63%, the concentration are 31.37mg/L, 5.15㎎/L, 6.60㎎/L and 1.10㎎/L separately. All of these satisfy theⅠ-B criteria specified in Discharge Standard of Pollutants for Municipal Wastewater Treatment Plant (GB 18918-2002). The system of SBBR-BAF run stably, removal sewage perfectly, avoid return and mud, save energy consumption.

Estimations of municipal point source pollution in the context of river basin management

2005 ◽  
Vol 52 (9) ◽  
pp. 175-182 ◽  
Author(s):  
M. Zessner ◽  
S. Lindtner
Keyword(s):  
Phosphorus Removal ◽  
Municipal Wastewater ◽  
Integrated Management ◽  
Treatment Plant ◽  
River Basin Management ◽  
Point Sources ◽  
Catchment Scale ◽  
Nitrogen And Phosphorus ◽  
Sources Of Pollution ◽  
Point And Diffuse Sources

Integrated presentation of total emissions on catchment scale is prerequisite for many tasks in integrated management of point and diffuse sources of pollution. This paper will focus on emissions of nutrients from municipal point sources. Based on calculations of discharges of N, P from households into wastewater and on the detailed evaluation of data from 76 municipal wastewater treatments plants, this paper presents ranges of specific loads of inhabitants and population equivalents in the raw wastewater. In addition data of these treatment plants have been evaluated in respect of the treatment efficiency for nitrogen and phosphorus (average reduction rates) dependent on the design characteristic (with or without nitrification, denitrification or enhanced phosphorus removal). The results of the investigation show that the specific N and P loads from households in Austria lie within the range 1.6–2.0gP/(inhabitant.d) and 11–13gN/(inhabitant.d). The specific contribution of industries to municipal wastewater varies between 0.3 and 2.0gP/(pe.d) and 0 and 13gN/(pe.d) with average values of 1.3gP/(pe.d) and 6.5gN/(population equivalent (pe)/d). As average values for municipal wastewater (contributions from household and industry) this leads to specific influent loads of 1.5gP/(pe.d) and 8.8gN/(pe.d). Average treatment efficiencies of treatment plants are for instance 50% nitrogen removal in treatment plants with nitrification and 80% in treatment plants with nitrification/denitrification. For phosphorus a removal of about 85% can be expected where the treatment plant was designed for enhanced phosphorus removal. Finally a method for load estimations based on standard values as mentioned above was tested for the estimation of emission from municipal point sources of selected regions.

scholarly journals Upflow packed bed Anammox reactor used in two-stage deammonification of sludge digester effluent

2018 ◽  
Vol 78 (9) ◽  
pp. 1843-1851 ◽  
Author(s):  
İ. Çelen-Erdem ◽  
E. S. Kurt ◽  
B. Bozçelik ◽  
B. Çallı
Keyword(s):  
Removal Efficiency ◽  
Nitrogen Removal ◽  
Municipal Wastewater ◽  
Removal Rate ◽  
Treatment Plant ◽  
Packed Bed ◽  
Municipal Wastewater Treatment ◽  
Two Stage ◽  
N Removal ◽  
Total Nitrogen Removal

Abstract The sludge digester effluent taken from a full scale municipal wastewater treatment plant (WWTP) in Istanbul, Turkey, was successfully deammonified using a laboratory scale two-stage partial nitritation (PN)/Anammox (A) process and a maximum nitrogen removal rate of 1.02 kg N/m3/d was achieved. In the PN reactor, 56.8 ± 4% of the influent NH4-N was oxidized to NO2-N and the effluent nitrate concentration was kept below 1 mg/L with 0.5–0.7 mg/L of dissolved oxygen and pH of 7.12 ± 12 at 24 ± 4°C. The effluent of the PN reactor was fed to an upflow packed bed Anammox reactor where high removal efficiency was achieved with NO2-N:NH4-N and NO3-N:NH4-N ratios of 1.32 ± 0.19:1 and 0.22 ± 0.10:1, respectively. The results show that NH4-N removal efficiency up to 98.7 ± 2.4% and total nitrogen removal of 87.7 ± 6.5% were achieved.

Evolution of the Southpest Wastewater Treatment Plant

2000 ◽  
Vol 41 (9) ◽  
pp. 7-14
Author(s):  
A. Jobbágy ◽  
B. Literáthy ◽  
F. Farkas ◽  
Gy. Garai ◽  
Gy. Kovács
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Phosphorus Removal ◽  
Treatment Plant ◽  
Low Flow ◽  
Nitrate Content ◽  
Nitrogen And Phosphorus ◽  
Treated Effluent ◽  
Processing Wastes

The treated effluent of the Southpest Wastewater Treatment Plant is discharged into a small, low-flow branch of the Danube susceptible to eutrophication. The first, high-load activated sludge system with a hydraulic retention time of 2.5 hrs in the aerated basins, was installed here in 1966. The paper presents the evolution of the technology by illustrating the effects of the different changes carried out since 1991. Reconfiguration of the existing activated sludge basins connected originally in parallel into an arrangement of tanks in series increased the settleability of the sludge as well as the efficiency of COD removal significantly. Introduction of an anaerobic zone preceding the aerated basins facilitated biological excess phosphorus removal with a consequent release in the thickener and digester. Introducing lime addition into the recycled sludge processing wastes significantly improved the performance of the system. However, since there had been no provision built for eliminating the nitrate content of the recycled sludge, efficiency of phosphorus removal proved to be dependent on the eventually occurring nitrification. In order to achieve both an effective nitrogen and phosphorus removal the current technology established in 1999 applies a nitrification and a denitrification filter following the activated sludge unit and uses precipitation for phosphorus removal.

Comparison of nitrogen and phosphorus removal efficiency between two types of baffled vertical flow constructed wetlands planted with Oenanthe Javanica

2020 ◽  
Vol 81 (9) ◽  
pp. 2023-2032
Author(s):  
Jingqing Gao ◽  
Lei Yang ◽  
Rui Zhong ◽  
Yong Chen ◽  
Jingshen Zhang ◽  
...  
Keyword(s):  
Removal Efficiency ◽  
Phosphorus Removal ◽  
Sewage Treatment ◽  
Drinking Water Treatment ◽  
Domestic Sewage ◽  
Good Choice ◽  
Vertical Flow ◽  
Nitrogen And Phosphorus ◽  
Nitrogen And Phosphorus Removal ◽  
Oenanthe Javanica

Abstract The environmental problems related to rural domestic sewage treatment are becoming increasingly serious, and society is also concerned about them. A baffled vertical flow constructed wetland (BVFCW) is a good choice for cleaning wastewater. Herein, a drinking-water treatment sludge-BVFCW (D-BVFCW) parallel with ceramsite-BVFCW (C-BVFCW) planted with Oenanthe javanica (O. javanica) to treat rural domestic sewage was investigated, aiming to compare nitrogen and phosphorus removal efficiency in different BVFCWs. A removal of 23.9% NH4+-N, 24.6% total nitrogen (TN) and 76.7% total phosphorus (TP) occurred simultaneously in the D-BVFCW; 56.4% NH4+-N, 60.8% TN and 55.2% TP respectively in the C-BVFCW. The root and plant height increased by an average of 7.9 cm and 8.3 cm, respectively, in the D-BVFCW, and by 0.7 cm and 1.1 cm, respectively, in the C-BVFCW. These results demonstrate that the D-BVFCW and C-BVFCW have different effects on the removal of N and P. The D-BVFCW mainly removed P, while C-BVFCW mainly removed N.

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