scholarly journals Dynamic changes of bacterial community in activated sludge with pressurized aeration in a sequencing batch reactor

2017 ◽  
Vol 75 (11) ◽  
pp. 2639-2648 ◽  
Author(s):  
Yong Zhang ◽  
Wei-Li Jiang ◽  
Yang Qin ◽  
Guo-Xiang Wang ◽  
Rui-Xiao Xu ◽  
...  

This study aimed to investigate the organic removal efficiency and microbial population dynamics in activated sludge with pressurized aeration. The activated sludge was fed with synthetic wastewater composed of simple carbon source to avoid the effect of complex components on microbial communities. The pressurized acclimation process was conducted in a bench-scale sequencing batch reactor (SBR) under 0.3 MPa gage pressure. Another SBR was running in atmospheric environment as a control reactor, with the same operation parameters except for the pressure. Bacterial diversity was investigated by Illumina sequencing technology. The results showed that the total organic carbon removal efficiency of the pressurized reactor was significantly higher, while the mixed liquor suspended solids concentrations were much lower than those of the control reactor. Moderate pressure of 0.3 MPa had little effect on Alpha-diversity of bacterial communities due to the similar running conditions, e.g., feed water, solids retention time (SRT) and the cyclic change of dissolved oxygen (DO) concentrations. Although the relative percentage of the bacterial community changed among samples, there was no major change of predominant bacterial populations between the pressurized group and the control group. Pressurized aeration would have a far-reaching impact on microbial community in activated sludge when treating wastewaters being unfavorable to the dissolution of oxygen.

2015 ◽  
Vol 73 (1) ◽  
pp. 95-101 ◽  
Author(s):  
Guanglei Qiu ◽  
Sin-Yi Neo ◽  
Yen-Peng Ting

The effects of CeO2 nanoparticles (NPs) on the system performance and the bacterial community dynamics in a sequencing batch reactor (SBR) were investigated, along with the fate and removal of CeO2 NPs within the SBR. Significant impact was observed on nitrification; NH4+-N removal efficiency decreased from almost 100% to around 70% after 6 days of continuous exposure to 1.0 mg/L of CeO2 NPs, followed by a gradual recovery until a stable value of around 90% after 20 days. Additionally, CeO2 NPs also led to a significant increase in the protein content in the soluble microbial products, showing the disruptive effects of CeO2 NPs on the extracellular polymeric substance matrix and related activated sludge structure. Denaturing gradient gel electrophoresis analysis showed remarkable changes in the bacterial community structure in the activated sludge after exposure to CeO2 NPs. CeO2 NPs were effectively removed in the SBR mainly via sorption onto the sludge. However, the removal efficiency decreased from 95 to 80% over 30 days. Mass balance evaluation showed that up to 50% of the NPs were accumulated within the activated sludge and were removed with the waste sludge.


2009 ◽  
Vol 59 (3) ◽  
pp. 573-582 ◽  
Author(s):  
Xiao-ming Li ◽  
Dong-bo Wang ◽  
Qi Yang ◽  
Wei Zheng ◽  
Jian-bin Cao ◽  
...  

It was occasionally found that a significant nitrogen loss in solution under neutral pH value in a sequencing batch reactor with a single-stage oxic process using synthetic wastewater, and then further studies were to verify the phenomenon of nitrogen loss and to investigate the pathway of nitrogen removal. The result showed that good performance of nitrogen removal was obtained in system. 0–7.28 mg L−1 ammonia, 0.08–0.38 mg L−1 nitrite and 0.94–2.12 mg L−1 nitrate were determined in effluent, respectively, when 29.85–35.65 mg L−1 ammonia was feeding as the sole nitrogen source in influent. Furthermore, a substantial nitrogen loss in solution (95% of nitrogen influent) coupled with a little gaseous nitrogen increase in off-gas (7% of nitrogen influent) was determined during a typical aerobic phase. In addition, about 322 mg nitrogen accumulation (84% of nitrogen influent) was detected in activated sludge. Based on nitrogen mass balance calculation, the unaccounted nitrogen fraction and the ratio of nitrogen accumulation in sludge/nitrogen loss in solution were 14.6 mg (3.7% of nitrogen influent) and 0.89, respectively. The facts indicated that the essential pathway of nitrogen loss in solution in this study was excess nitrogen accumulation in activated sludge.


2012 ◽  
Vol 610-613 ◽  
pp. 1454-1458
Author(s):  
Ming Fen Niu ◽  
Hong Jing Jiao ◽  
Li Xu ◽  
Yan Yu ◽  
Jian Wei

A2N is two-sludge system, by using the method that first bringing up the cultivation of denitrifying phosphorus removing bacteria (DPB) and nitrification biofilm separately then connecting them, which can start up A2N system successfully. Nitrification biofilm was cultivated in a sequencing batch reactor (SBR). After 30 days, NH4+-N effluent concentration steadily stayed below 0.5mg·L-1.In another SBR, the activated sludge for the enrichment of DPB is from the anaerobic tank, which was firstly operated under anaerobic/aerobic (A/O) condition. After 20 days, PAOs was successfully enriched. Then, the activated sludge was conducted under anaerobic/anoxic/aerobic (A/A/O) condition, maintaining the anaerobic time, gradually increased anoxic time and induced aerobic time. After 30 days DPB was successfully enriched, two phases totally take 50 days. The removal efficiency of total nitrogen and phosphorus are above 85 % and 95 %, so that A2N system was started up successfully.


2015 ◽  
Vol 72 (9) ◽  
pp. 1653-1661 ◽  
Author(s):  
YangWei Yan ◽  
YuWen Wang ◽  
Yan Liu ◽  
Xiang Liu ◽  
ChenChao Yao ◽  
...  

The effects of synthetic wastewater that contained 20 mg/L Cu(II) on the removal of organic pollutants in a sequencing batch reactor were investigated. Results of continuous 20 mg/L Cu(II) exposure for 120 days demonstrated that the chemical oxygen demand (COD) removal efficiency decreased to 42% initially, followed by a subsequent gradual recovery, which peaked at 78% by day 97. Effluent volatile fatty acid (VFA) concentration contributed 67 to 89% of the influent COD in the experimental reactor, which indicated that the degradation of the organic substances ceased at the VFA production step. Meanwhile, the varieties of soluble microbial products (SMP) content and main components (protein, polysaccharide, and DNA) were discussed to reveal the response of activated sludge to the toxicity of 20 mg/L Cu(II). The determination of Cu(II) concentrations in extracellular polymeric substances (EPS) and SMP throughout the experiment indicated an inverse relationship between extracellular Cu(II) concentration and COD removal efficiency.


2012 ◽  
Vol 19 (3) ◽  
pp. 405-413
Author(s):  
Mirosław Szyłak-Szydłowski

Effectiveness of Removal of Humic Substances and Heavy Metals from Landfill Leachates During their Pretreatment Process in the SBR ReactorIn the paper the removal efficiency of heavy metals as well as humic compounds, in the treatment of leachate mixed with municipal waste in a sequencing batch reactor was studied. Also, the accumulation of those metals in the activated sludge was examined. It has been shown that the removal efficiency of contamination with humic compounds, forBxranging from 0.23 to 0.45 mg COD mg-1d.m. can reach 71÷74%. An increase in the concentrations of heavy metals in the activated sludge was recorded forBxin the range 0.23÷1.64 mg COD mg-1d.m. The amount of heavy metals in the effluent of the SBR in carrying out the process atBx= 0.23÷0.96 mg COD mg-1d.m. does not limit their discharge into water and sewer system.


2017 ◽  
Vol 76 (5) ◽  
pp. 1044-1058 ◽  
Author(s):  
Amir Mohammad Mansouri ◽  
Ali Akbar Zinatizadeh

The performance of two bench scale activated sludge reactors with two feeding regimes, continuous fed (an up-flow aerobic/anoxic sludge fixed film (UAASFF) bioreactor) and batch fed (sequencing batch reactor (SBR)) with intermittent aeration, were evaluated for simultaneous nutrients (N, P) removal. Three significant variables (retention/reaction time, chemical oxygen demand (COD): N (nitrogen): P (phosphorus) ratio and aeration time) were selected for modeling, analyzing, and optimizing the process. At high retention time (≥6 h), two bioreactors showed comparable removal efficiencies, but at lower hydraulic retention time, the UAASFF bioreactor showed a better performance with higher nutrient removal efficiency than the SBR. The experimental results indicated that the total Kjeldahl nitrogen removal efficiency in the UAASFF increased from 70.84% to 79.2% when compared to SBR. It was also found that the COD removal efficiencies of both processes were over 87%, and total nitrogen and total phosphorus removal efficiencies were 79.2% and 72.98% in UAASFF, and 71.2% and 68.9% in SBR, respectively.


2013 ◽  
Vol 781-784 ◽  
pp. 2047-2050
Author(s):  
Xiu Qiong Guan ◽  
Hong Xia Gao ◽  
Tian Xue Lin ◽  
Chun Liu

Sequencing Batch Reactor (SBR) was investigated for the treatment of liquor wastewater in this paper. This study includes the test of start-up of the SBR, running of the reactor by treating the wastewater. During the start-up process the activated sludge was cultured and acclimated. After acclimation the removal efficiency of CODcr can reach around 99%. Following investigations were focused on evaluation of the treatment efficiency and the reactive cycle during running the SBR. The experimental results showed that running parameters of SBR reaction system were Fill 0.5 h, React 10.0 h, Settle 2.0~2.5 h, Drain 0.5h, Idle 4.0h. Under these conditions the removal rate of CODcr can reach 99%. So the performance of SBR for the wastewater was satisfactory as the wastewater had 1400~2000mg/l of CODcr. The cyclic operation of SBR used in this study proved more effective in treating the wastewater.


2017 ◽  
Vol 244 ◽  
pp. 872-879 ◽  
Author(s):  
Qiang Kong ◽  
Xiao He ◽  
Shuai-shuai Ma ◽  
Yu Feng ◽  
Ming-sheng Miao ◽  
...  

1986 ◽  
Vol 18 (7-8) ◽  
pp. 363-370 ◽  
Author(s):  
M. Okada ◽  
R. Sudo

Laboratory-scale sequencing batch reactor (SBR) activated sludge process was fed with synthetic wastewater to clarify the possibility of simultaneous removal of nitrogen, phosphorus, and organic substances by modifying operational schedule. Anoxic and/or anaerobic reactions in addition to aerobic reactions were introduced into a cycle of batch operation. Simultaneous removal of nitrogen, phosphorus and organic carbon was shown to be possible by the modified operation of SBR into which reactions without aeration (anoxic/anaerobic) were introduced during the fill period. Oxidized nitrogen remaining in the reactor at the end of the former cycle was removed by denitrification during the fill. Anaerobic conditions after the denitrification was completed enhanced phosphorus release from the sludge and the following luxury uptake during aeration removed phosphorus from water. Both extension and reduction of anoxic/anaerobic period in the beginning of a cycle damaged phosphorus removal and sludge settlability. Thus, an optimum length of period for these reactions must be chosen for successful operations. The best length of this period coincided with that of the fill within the range of this study.


2012 ◽  
Vol 550-553 ◽  
pp. 2100-2103
Author(s):  
Jun Li ◽  
Xiu Qiong Guan ◽  
Chun Liu

ABSTRACT. SBR (Sequencing Batch Reactor) was investigated for the treatment of OCC pulping wastewater in this paper. This study includes the design of the SBR device and its control system, the test of start-up of the SBR, running of the reactor by treating the wastewater. During the start-up process the activated sludge was cultured and acclimated. After acclimation the removal efficiency of CODcr can reach around 80%. Following investigations were focused on evaluation of the treatment efficiency and the reactive cycle during running the SBR. The experimental results showed that the running parameters of SBR reaction system were Fill 2.0 h, React 12.0 h, Settle 1.0~2.0 h, Drain 0.5h, Idle 4.0h. Under these conditions the removal rate of COD can reach 94%. So the performance of SBR for the wastewater was satisfactory as the wastewater had 1500~2500mg/l of COD. The cyclic operation of SBR used in this study proved more effective in treating the wastewater.


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