scholarly journals Bioremediation of methylene blue dye using Bacillus subtilis MTCC 441

2017 ◽  
Vol 75 (7) ◽  
pp. 1572-1583 ◽  
Author(s):  
Ganta Upendar ◽  
Susmita Dutta ◽  
Pinaki Bhattacharya ◽  
Abhishek Dutta

Methylene blue (MB) commonly found in the textile industry effluent has been chosen as a model dye to investigate bioremediation using Bacillus subtilis MTCC 441. Both free cells and calcium alginate immobilized cells have been used to remove MB from the effluent. The operating variables of initial concentration of dye (20–60 mg/L), inoculum size (4–8%) and temperature (25–35 °C) have been varied judiciously during the kinetic study in a batch contactor. A maximum removal of 91.68% is obtained when 20 mg/L MB solution was inoculated with 8% inoculum and cultured for 6 h at 30 °C. Continuous removal of MB has been studied in a fixed bed contactor using immobilized cells as packing materials. Influent concentration (10–30 mg/L) was varied and breakthrough parameters have been determined. With increase in influent concentration from 10 mg/L to 30 mg/L, percentage removal of dye decreases from 72.44% to 49.62%.

2013 ◽  
Vol 65 (1) ◽  
Author(s):  
Norzita Ngadi ◽  
Chin Chiek Ee ◽  
Nor Aida Yusoff

Dyes contain carcinogenic materials which can cause serious hazards to aquatic life and the users of water. Textile industry is the main source of dye wastewater which results in environmental pollution. Many studies have been conducted to investigate the use of low cost adsorbent as an alternative technique for the adsorption of dye. The objective of this study is to determine the potential of eggshell powder as an adsorbent for methylene blue removal and find out the best operating conditions for the color adsorption at laboratory scale. The adsorption of cationic methylene blue from aqueous solution onto the eggshell powder was carried out by varying the operating parameters which were contact time, pH, dosage of eggshell powder and temperature in order to study their effect in adsorption capacity of eggshell powder. The results obtained showed that the best operating condition for removal of methylene blue was at pH 10 (78.98 %) and temperature 50°C (47.37 %) by using 2 g of eggshell powder (57.03 %) with 30 minutes equilibrium time (41.36 %). The kinetic studies indicated that pseudo-second-order model best described the adsorption process.


2021 ◽  
Vol 7 (2) ◽  
pp. 188-195
Author(s):  
Nurhasni Nurhasni ◽  
Sariana Harahap ◽  
Ahmad Fathoni ◽  
Hendrawati Hendrawati

The ability of bagasse adsorbents to adsorb methylene blue without activation using 0.5 M H2SO4 solution was examined. Methylene blue is widely used in the textile industry because it produces bright colors, and the dyeing process is fast and easy. This research aims to determine the optimum adsorption conditions, namely the variations in contact time, dye concentration, adsorbent mass, and pH effect on methylene blue, which were carried out using the batch method. Furthermore, the adsorbents were characterized by FT-IR and SEM. The optimum state of the bagasse adsorbent to adsorb methylene blue dye has a mass of 0.5 grams, a contact time of 30 minutes, a concentration of 50 ppm, and a pH of 5. The character of the adsorbent after activation with H2SO4 was better than without activation. The highest adsorption efficiency of methylene blue dye in the batch method was 99.67%. The FTIR spectrum of the bagasse adsorbent showed OH, C-H, C=O, C=C, and C-O functional groups. The adsorption isotherm model for methylene blue dye follows the Langmuir isotherm since the graph obtained is linear with the correlation coefficient (R2) = 1, where the adsorbent has a homogeneous surface.


2020 ◽  
Vol 24 (4) ◽  
pp. 04020037 ◽  
Author(s):  
Suraj Punathil ◽  
Damodhar Ghime ◽  
Titikshya Mohapatra ◽  
Chandrakant Thakur ◽  
Prabir Ghosh

2021 ◽  
Vol 17 (4) ◽  
pp. 707-716
Author(s):  
Hema Gunti ◽  
Vyshali Venkatappa Maruthiramaih ◽  
Tippeswamy Boreddy Shivanandappa

Untreated effluents from the textile industry affect aquatic life irreversibly. Synthetic dyes not only change the color of water resources but also make them hazardous.The main objective of the study was to evaluate the decolorizing potential of a new isolate of Bacillus subtilis from soil samples contaminated with industrial effluent in and around textile industrial area in South Karnataka. This isolate of Bacillus subtilis has high decolorizing potential and took only 24 hrs for complete decolorization of acid orange-10 azo dye at 200ppm. Different parameters like temperature, pH, aeration, dye concentration and inoculum size were optimized for complete decolorization of Acid orange-10 azo dye by this isolate of Bacillus subtilis. The dye was completely decolorized at 400C within 24 hrs and it was capable of decolorizing 700 ppm dye in 72 hrs. Optimum pH was found to be 8.5 and maximum decolorization was achieved under static conditions. As the inoculum size increased, the time taken for complete decolorization of Acid orange-10 dye was decreased from 36 hrs at 1% to 16 hrs at 10% of inoculum size. The new isolate decolorizes 100 ppm of dye completely (i.e.100%) within 12hrs of incubation. The time taken for the complete decolorization increased with increase in the concentration of Acid orange-10 azo dye. In conclusion, the new isolate of Bacillus subtilis from soil samples contaminated with textile industrial effluent was found to be a potential candidate for decolorization of Acid orange-10 azo dye in textile effluents.


Author(s):  
Iliane Muller Otto ◽  
Luiza Beatriz Gamboa Araújo Morselli ◽  
Dienifer Aline Braun Bunde ◽  
Simone Pieniz ◽  
Maurízio Silveira Quadro ◽  
...  

The textile industry, very important for the world economy, generates an effluent containing dyes, and which, when discarded in water bodies without proper treatment, can cause impacts to human health and the environment. One of these widely used dyes is methylene blue, whose characteristics are high solubility in water and its toxic potential, and which effects range from eye irritations, nausea, vomiting and even mental confusion. Among the potential adsorbents of this dye is chitin, which is a biopolymer extracted from the shrimp exoskeleton. Aiming at the development of a low-cost adsorbent material with potential use in the textile effluent treatment industry, the ability to remove methylene blue dye by shrimp residue chitin, obtained by eleven different methodologies, was verified. The three most efficient treatments reached approximately 75% of dye removal, proving the high adsorption power of shrimp residue. In addition to providing technological development of materials, the research brings socioeconomic benefits to the fishermen’s colony with the use of shrimp residue for the adsorption of other waste from the textile industry, contributing to the sustainability of both activities and reducing the environmental impact.


2020 ◽  
Vol 36 (6) ◽  
pp. 127-137
Author(s):  
O.A. Zhuravliova ◽  
T.A. Voeikova ◽  
V.G. Debabov

It has been demonstrated for the first time that biogenic nanoparticles of cadmium sulfide (NPsCdS) obtained using the bacterial strain Bacillus subtilis 168 by an optimized environmentally friendly, nature-like microbial synthesis are able to photocatalytically decolorize methylene blue dye under UV irradiation (365 nm). NPsCdS, characterized as quantum dots, fluoresce in the blue spectral region (400-440 nm), have a spherical shape, a diameter of 5±1 nm and a hydrodynamic diameter of 250--300 nm. The dynamics of methylene blue decolorization was studied depending on the NPsCdS and the dye concentrations, the thickness of the dye solution layer, as well as the power and time of exposure to UV irradiation. The possibility of reusing biogenic NPsCdS for dye decolorization was shown, which characterizes the photostability of the nanoparticles and is relevant for the practical application of biogenic photonanocatalysts. biogenic CdS nanoparticles, Bacillus subtilis 168, photocatalysis, UV irradiation, methylene blue The authors are grateful to the Russian National Collection of Industrial Microorganisms of NRC "Kurchatov Institute" - GOSNIIGENETIKA for providing the bacterial strains and to the Kurchatov Genomic Center-NRC "Kurchatov Institute"- GosNIIGENETIKA for the opportunity to determine the photocatalytic degradation of the dye using biogenic nanoparticles on spectrophotometric equipment. This work was supported by the Russian Foundation for Basic Research in the framework of project no. 19-04-00088.


2021 ◽  
Author(s):  
NORAIN ISA ◽  
Mohamed Syazwan Osman ◽  
Haslinda Abdul Hamid ◽  
Nurdiana Samsulrizal ◽  
Vicinisvarri Inderan ◽  
...  

Abstract The textile industry is a heavy producer of wastewater, which may result in the discharge of toxic dyes into the environment. Methylene blue (MB) is an example of the most used dye in the textile industry. It is difficult to degrade MB under normal conditions due to its highly stable molecules. Therefore, a catalyst route is desired in MB reduction. The catalyst chosen in this work was silver nanoparticles (AgNPs) synthesised by a biological method utilising shortleaf spikesedge extract (SSE) as a reducing agent. The formations of SSE driven AgNPs were monitored using visual observation (colour), ultraviolet-visible spectroscopy (UV-vis), and transmission electron microscopy (TEM). The different process variables (concentration of AgNO3, concentration of SSE, reaction time, temperature and pH) upon synthesis of SSE driven AgNPs were evaluated based on the absorbance of surface plasmon resonance (SPR) band. The TEM image showed that SSE driven AgNPs are highly dispersed with a quasi-spherical shape and an average particle size of approximately 17.64 nm. For the catalytic study, the reduction of MB was evaluated using two systems. A detailed batch study of the removal efficiency (%RE) and kinetics was done at ambient temperature, various MB initial concentrations, and reaction time. The batch study for System 2 clearly showed that SSE driven AgNPs exhibited 100% reduction of MB at 30–100 mg/L initial concentration (sample coding of MB30, MB50, MB70 and MB100) between 1.5 and 5.0 min reaction time. The kinetic data best fitted a pseudo-first-order kinetic model with the highest reaction rate of 2.5715 min-1. The reduction of MB occurs via the electron relay effect. These findings demonstrate that the SSE driven AgNPs are a promising candidate with potential influence on coloured wastewater.


2017 ◽  
Vol 65 ◽  
pp. 346-358 ◽  
Author(s):  
V. Makrigianni ◽  
A. Giannakas ◽  
D. Hela ◽  
M. Papadaki ◽  
I. Konstantinou

2019 ◽  
Vol 213 ◽  
pp. 884-891 ◽  
Author(s):  
Mayur B. Kurade ◽  
Tatoba R. Waghmode ◽  
Jiu-Qiang Xiong ◽  
Sanjay P. Govindwar ◽  
Byong-Hun Jeon

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