scholarly journals Erratum: Water Science and Technology 72 (11), 1962–1972: Potential applications of next generation DNA sequencing of 16S rRNA gene amplicons in microbial water quality monitoring, J. Vierheilig, D. Savio, R. E. Ley, R. L. Mach, A. H. Farnleitner and G. H. Reischer, doi: 10.2166/wst.2015.407

2016 ◽  
Vol 73 (7) ◽  
pp. 1768-1768
Keyword(s):  
Water Quality ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Dna Sequencing ◽  
Water Quality Monitoring ◽  
Quality Monitoring ◽  
Rrna Gene ◽  
Microbial Water Quality ◽  
Next Generation Dna Sequencing ◽  
Potential Applications

scholarly journals Potential applications of next generation DNA sequencing of 16S rRNA gene amplicons in microbial water quality monitoring

2015 ◽  
Vol 72 (11) ◽  
pp. 1962-1972 ◽  
Author(s):  
J. Vierheilig ◽  
D. Savio ◽  
R. E. Ley ◽  
R. L. Mach ◽  
A. H. Farnleitner ◽  
...  
Keyword(s):  
Water Quality ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Dna Sequencing ◽  
Bacterial Communities ◽  
Bacterial Community Composition ◽  
Detection Methods ◽  
Rrna Gene ◽  
Next Generation ◽  
Next Generation Dna Sequencing

The applicability of next generation DNA sequencing (NGS) methods for water quality assessment has so far not been broadly investigated. This study set out to evaluate the potential of an NGS-based approach in a complex catchment with importance for drinking water abstraction. In this multi-compartment investigation, total bacterial communities in water, faeces, soil, and sediment samples were investigated by 454 pyrosequencing of bacterial 16S rRNA gene amplicons to assess the capabilities of this NGS method for (i) the development and evaluation of environmental molecular diagnostics, (ii) direct screening of the bulk bacterial communities, and (iii) the detection of faecal pollution in water. Results indicate that NGS methods can highlight potential target populations for diagnostics and will prove useful for the evaluation of existing and the development of novel DNA-based detection methods in the field of water microbiology. The used approach allowed unveiling of dominant bacterial populations but failed to detect populations with low abundances such as faecal indicators in surface waters. In combination with metadata, NGS data will also allow the identification of drivers of bacterial community composition during water treatment and distribution, highlighting the power of this approach for monitoring of bacterial regrowth and contamination in technical systems.

scholarly journals Astacopsidrilus hibernicus sp. nov. (Phreodrilidae, Oligochaeta, Annelida) from Irish peatlands

Zoosymposia ◽  
2020 ◽  
Vol 17 (1) ◽  
pp. 34-44
Author(s):  
RÜDIGER M. SCHMELZ ◽  
MÅRTEN J. KLINTH ◽  
RACHEL WISDOM ◽  
THOMAS BOLGER
Keyword(s):  
United Kingdom ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Dna Sequencing ◽  
Dorsal Side ◽  
Rrna Gene ◽  
The United Kingdom ◽  
The 16S Rrna Gene ◽  
Epidermal Gland ◽  
Whole Mounts

The discovery of a large and flourishing population of Phreodrilidae in terrestrial peatlands in northwest Ireland was surprising on two counts: these oligochaete worms are usually aquatic and most of the species occur in the Southern Hemisphere. The phreodrilids were discovered in a project that targeted Enchytraeidae, therefore methods adapted to the investigation of enchytraeids could be applied, including the study of living animals and properly fixed whole mounts. DNA sequencing was also performed. All worms identified here belong to one species, new to science, and placed in the genus Astacopsidrilus, because of the ventral position of the spermathecal pores and the opening of the female funnels inside the spermathecal vestibule. Astacopsidrilus hibernicus sp. nov. is mainly distinguished by thick segmental cushions of epidermal gland cells on the dorsal side of the posterior body half. Male sexual organs and spermathecae are comparatively small and without the often-observed bizarre modifications common in species of this family. DNA sequencing yielded a fragment of the 16S rRNA gene. This is the first description of a phreodrilid species from Europe; the few previous recordings of this family in Ireland and the United Kingdom had been left unidentified.

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