Paraben resistance in bacteria from sewage treatment plant effluents in India

2013 ◽  
Vol 68 (9) ◽  
pp. 2067-2073 ◽  
Author(s):  
Krishna Kumar Selvaraj ◽  
Senthilkumari Sivakumar ◽  
Srimurali Sampath ◽  
Govindaraj Shanmugam ◽  
Umamaheswari Sundaresan ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Bacterial Resistance ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Diffusion Method ◽  
Continuous Exposure ◽  
Gram Negative Bacteria ◽  
Gram Positive Bacteria ◽  
Receiving Waters

Parabens, the antimicrobial preservatives used in cosmetics, food and pharmaceuticals, are often detected in the aquatic environment. Generally, sewage treatment plants (STPs) receive community sewage containing parabens, which are ultimately released into streams/rivers. In this study, bacteria in STP effluents were evaluated for their resistance to parabens. The susceptibility was in the order of Staphylococcus aureus > Bacillus sp. >Escherichia coli > Pseudomonas aeruginosa. Gram-negative bacteria showed less susceptibility than their control and Gram-positive bacteria. Further, the bacteria were more sensitive towards butyl and ethyl parabens. Interestingly, the strains showed resistance to ≥5 mg of parabens, which is equivalent to or higher than reported environmental concentrations. The increase in paraben chain length did not enhance the susceptibility in all cases and it was understood that the activity may differ for each bacterium in the environment. This is the first profile on paraben resistance in common pathogens of Indian STPs. Paraben resistance may be developed due to continuous exposure even at sub-inhibitory and/or chronic levels in the environment and this resistance may be transferred to other pathogenic bacteria in receiving waters. Thus the study demonstrates the effectiveness of the disc diffusion method in environmental bacterial resistance assessment and addresses the risk involved in the use of parabens.

scholarly journals Bacteriological, Physical And Chemical Properties Of The Pagla Sewage Treatment Plant’s Water

1970 ◽  
Vol 21 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Mihir Lal Saha ◽  
Ashraful Alam ◽  
Mahbubar Rahman Khan ◽  
Sirajul Hoque
Keyword(s):  
Heterotrophic Bacteria ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Chemical Properties ◽  
Bacillus Sphaericus ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Treated Water

Samples from Pagla sewage treatment plant at different treatment stages showed more or less similar temperature (26.9 - 27.5°C). The pH ranged from 7.2 - 7.9. Influent water and primary sedimentation tank water were brownish in colour while sludge water was light black. The lagoon water and treated water were greenish. The ammonium?nitrogen (NH+4-N) and nitrate?nitrogen (NO-3?N) ranged from 5.24 - 61.94 mg/l and 2.55 - 11.02 mg/l, respectively. Phosphorus of the water was 1.34 - 4.50 mg/l. The suspended solids (SS) ranged from 25.48 ? 374.69 mg/l. In the present study the amount of SS in the treated water were found to be quite satisfactory. The total bacterial population in Pagla sewage treatments plant was in between 2.9 × 104 and 2.5 × 106 cfu/ml. The qualitative bacterial spectrum showed a potential consortium of bacteria associated with the treatment plant. Both Gram positive and Gram negative bacteria were present. Gram positive bacteria were represented by the different species of the genus Bacillus, Aureobacterium and Kurthia. Among them Bacillus was the dominant genus. The different species of Bacillus were Bacillus sphaericus, B. fastidiosus, B. circulans and B. pasteurii. Gram negative bacteria were Zoogloea, Yersinia, Citrobacter and Pseudomonas. A good number of microorganisms were found to be associated with the bio-oxidation of the organic compounds of the influent. The Zoogloea along with other free flowing aerobic heterotrophic bacteria like Bacillus, Pseudomonas could play the major role in the sewage treatment.DOI: http://dx.doi.org/10.3329/dujbs.v21i1.9739 DUJBS 2012 21(1): 1-7

scholarly journals Microbial air contamination on the premises of the sewage treatment plant in Bydgoszcz (Poland) and antibiotic resistance of Staphylococcus spp.

2017 ◽  
Vol 43 (4) ◽  
pp. 58-65 ◽  
Author(s):  
Marta Małecka-Adamowicz ◽  
Łukasz Kubera ◽  
Wojciech Donderski ◽  
Katarzyna Kolet
Keyword(s):  
Heterotrophic Bacteria ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Antibiotic Sensitivity ◽  
Primary Treatment ◽  
Average Concentration ◽  
Diffusion Method ◽  
Air Contamination ◽  
Staphylococcus Spp

AbstractThe study was aimed at evaluating microbial contamination on the premises of the sewage treatment plant by determining the concentrations of selected groups of airborne microorganisms. Another objective was to determine the antibiotic sensitivity patterns of isolated strains of staphylococci. The research was conducted in a seasonal cycle, by the impaction method using Merck MAS-100 air sampler. Samples were collected at six sites, each representing a different stage of sewage treatment. The susceptibility of isolated staphylococci was assessed with the disc-diffusion method, following the recommendations of the EUCAST. The results indicate that the microbial population in the air of the investigated area was dominated by mold fungi, whose highest average concentration was recorded at site IV located near the final clarifier (7672 CFU•m-3). Heterotrophic bacteria and mannitol-positive staphylococci were the most numerous at locations where sewage undergoes primary treatment. In each subseuqent stage the number of microorganisms emitted into the air from the sewage was lower. Antibiograms show that more than 50% of Staphylococcus spp. exhibited resistance to penicillin and 20% to rifampicin. In addition, 90% of the analyzed strains were sensitive to other antibiotics. The fungal community included the following genera: Cladosporium, Fusarium, Alternaria, Penicillium, Aspergillus, Aureobasidium, and Acremonium.The highest air contamination with all studied groups of microorganisms was recorded at the locations where mechanical sewage treatment was performed. During the subsequent stages lower numbers of heterotrophic bacteria were emitted into the air. The air in the investigated sewage treatment plant did not contain multidrug-resistant staphylococci.

scholarly journals Distributions of Microplastics in Surface Water, Fish, and Sediment in the Vicinity of a Sewage Treatment Plant

Water ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3333
Author(s):  
Tae-Jin Park ◽  
Seung-Hyun Lee ◽  
Myung-Sung Lee ◽  
Jae-Kwan Lee ◽  
Ji-Hyoung Park ◽  
...  
Keyword(s):  
Surface Water ◽  
Rainy Season ◽  
Wastewater Treatment Plants ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Dry Season ◽  
Water Fish ◽  
Receiving Waters ◽  
The Impact

Microplastics are ubiquitously found in freshwater and marine environments worldwide. In particular, wastewater treatment plants (WWTPs) or sewage treatment plants (STPs) have been recognized as a main source of microplastics in the receiving freshwater. However, only a few studies have been conducted to examine the impact of these facilities on receiving waters. In this study, we investigated the distribution of microplastics in surface water, fish, and sediment near a sewage treatment plant (STP) in the Tanchon stream, one of the main tributaries flowing into the Han River, Korea. The concentration of microplastics in water varied spatially and temporarily, ranging between 5.3 and 87.3 particles/m3 (31.4 ± 28.5 particles/m3). In fish, the concentration in upstream and downstream sites was 7.3 ± 7.3 and 12.4 ± 17.9 particles/fish, respectively. Spatially, the downstream site was the most polluted with microplastics in water and fish. The concentration of microplastics was positively correlated with fish body length and weight. In sediment, microplastic concentration in upstream and downstream sites was 493.1 ± 136.0 and 380.0 ± 144.2 particles/kg, respectively. The contribution of upstream to the microplastic load in downstream was 15.8% in dry season (April), which was higher than that of STP effluent and Yangjaechon creek. Meanwhile, the highest load was observed in STP effluent (5.1%) in rainy season (August). Microplastics were more abundant in water in the rainy season (37.4 ± 37.0 particles/m3) than in the dry season (28.2 ± 22.2 particles/m3). Polyethylene (49%) and polypropylene (18%) were the most abundant polymer types in water, fish, and sediment. Regarding shape of microplastics, fragments were dominant (95%) over fiber and film in water, fish, and sediment.

scholarly journals Test for the antibacterial inhibition of kaffir lime leaf (Citrus hysteric D.C) extract against pathogen bacteria in improving food safety

2021 ◽  
Vol 883 (1) ◽  
pp. 012056
Author(s):  
S J Nendissa ◽  
D M Nendissa
Keyword(s):  
Antibacterial Activity ◽  
Food Safety ◽  
Salmonella Typhimurium ◽  
Pathogenic Bacteria ◽  
Leaf Extract ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria

Abstract Kafir lime leaf (Citrus hystrix) is a plant from the citrus tribe that has long been known by community as flavor ingredient. To support its use and increase its application in supporting food safety, a test the inhibition of on kaffir lime leaf extract against pathogenic bacteria, namely Gram Negative Bacteria (Escherichia coli, Salmonella typhimurium) and Gram Positive bacteria (Staphylococcus aereus, P. aeroginosa). Making kaffir lime leaf extract (Citrus hystrix) was done by weighing 150g of lime leaf powder, then immersing in 96% ethanol solution and leaving for + 3 days. Kaffir lime leaf extract was dissolved with sterile distilled aquades to obtain a concentration of 5%, 10% and 15%. The antibacterial activity of kaffir lime leaf extract was tested by diffusion method using disc paper to determine of the bacterial growth inhibition area. The results showed that kaffir lime extract had antibacterial activity inhibition of 12,78 mm of S. aereus, 9 mm of E.coli, 7,12 mm of S. typhimurium and 9,3 mm of P. aeroginosa. Kaffir lime leaf extract has inhibition effectiveness for gram positive bacteria Staphylococcus aereus and gram negative bacteria E. coli, Salmonella typhimurium, P. aeroginosa. Thus, kaffir lime leaf extract can be used as a decontaminant agait theses 4 type of bacteria, especially Staphylococcus aereus which has a strong inhibitory power, so it can maintain quality and increase the safety of mead based foods

scholarly journals Influence of Sewage Treatment Plant Effluent on the Presence of Culturable Pathogenic Bacteria in the Water Body

2021 ◽  
Vol 18 (1) ◽  
pp. 173-184
Author(s):  
Gulsanga Lemar ◽  
Saleha Shahar ◽  
Abdul Rahman i Osman
Keyword(s):  
Health Risk ◽  
Membrane Filtration ◽  
Pathogenic Bacteria ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Treated Wastewater ◽  
Resistant Bacteria ◽  
Culturable Bacteria ◽  
Filtration Method

The purpose of the sewage treatment process is to decrease the concentration of contaminants, including pathogens, before discharging into the receiving streams. And the standard operating procedure of STP in Malaysia is to discharge the treated wastewater with low nutrient and low organic materials into the streams but the bacterial content of the discharge and its risk to the stream’s natural microbial verity or health is unknown. However, studies reported that pathogens could escape sewage treatment plants (STPs) processes and showed health risk of streams impacted by STP effluent. On the other hand, majority of these studies relied on metagenomic strategy, without assessing changes to culturable bacteria. Isolation of living microbes provides realistic risk assessment compared to metagenome survey alone. Therefore, this study aims to determine the presence of culturable pathogenic bacteria from water impacted by STP effluent to establish justifiable public health risk. For that, the presence of bile resistant bacteria was determined from water taken from surface water receiving effluent from STP-1 (Kolej 9, UTM) in Malaysia. Enumeration and isolation of bacteria were done on MacConkey agar through membrane filtration method, followed by partial identification, using Triple Sugar Iron agar (TSI). The result showed that STP effluent changes the diversity, and abundance, of bile resistant bacteria (specifically Enterobacteriaceae family) of receiving streams. Most of the isolated bile resistant bacteria are opportunistic pathogens for human. Findings from this study provide a snapshot of the bigger picture of microbial changes in a stream impacted by STP effluent painted initially by metagenome studies. And shows that despite of treatment, some contaminants (microbes) remained and released into surface waters, which contribute to the water pollutions.

Analysis and balance of phosphorous removal from wastewater at urban wastewater treatment plant

2020 ◽  
Vol 15 (2) ◽  
pp. 142-151
Author(s):  
Peter Lukac ◽  
Lubos Jurik
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Wastewater Treatment Plants ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Phosphorus Recovery ◽  
Anaerobic Sludge ◽  
Biological Phosphorus Removal ◽  
The Mean

Abstract:Phosphorus is a major substance that is needed especially for agricultural production or for the industry. At the same time it is an important component of wastewater. At present, the waste management priority is recycling and this requirement is also transferred to wastewater treatment plants. Substances in wastewater can be recovered and utilized. In Europe (in Germany and Austria already legally binding), access to phosphorus-containing sewage treatment is changing. This paper dealt with the issue of phosphorus on the sewage treatment plant in Nitra. There are several industrial areas in Nitra where record major producers in phosphorus production in sewage. The new wastewater treatment plant is built as a mechanicalbiological wastewater treatment plant with simultaneous nitrification and denitrification, sludge regeneration, an anaerobic zone for biological phosphorus removal at the beginning of the process and chemical phosphorus precipitation. The sludge management is anaerobic sludge stabilization with heating and mechanical dewatering of stabilized sludge and gas management. The aim of the work was to document the phosphorus balance in all parts of the wastewater treatment plant - from the inflow of raw water to the outflow of purified water and the production of excess sludge. Balancing quantities in the wastewater treatment plant treatment processes provide information where efficient phosphorus recovery could be possible. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. The mean daily value of P tot is approximately 122.3 kg/day of these two sources. There are also two outflows - drainage of cleaned water to the recipient - the river Nitra - 9.9 kg Ptot/day and Ptot content in sewage sludge - about 120.3 kg Ptot/day - total 130.2 kg Ptot/day.

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