Optimization of the nutrition for biodegradation of vinasse by Aspergillus oryzae using response surface methodology

2013 ◽  
Vol 67 (4) ◽  
pp. 772-779 ◽  
Author(s):  
Zhicai Zhang ◽  
Dan Liu ◽  
Fan Feng ◽  
Jiashao Li ◽  
Ming Li ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Vitamin B6 ◽  
Oxygen Demand ◽  
Organic Substrates ◽  
Box Behnken Design ◽  
Design Expert ◽  
Environmental Destruction ◽  
Model Equations ◽  
Direct Discharge ◽  
Develop Mathematical Model

Direct discharge of vinasse from the distillery industry causes resource wasting and environmental destruction due to its mass of organic components. Aspergillus oryzae CGMCC5992 is capable of degrading the organic substrates of wastewater. One-factor-at-a-time design was adopted to select the most important nutrients influencing the degradation of organic materials of vinasse. Box–Behnken Design (BBD) with Design-Expert (8.0.4) was used to develop mathematical model equations, study responses, and optimize concentrations of the key nutrients to improve the degradation efficiency. The optimized medium containing 0.3 g/L urea, 20.73 mg/L ZnSO4, and 19.79 mg/L vitamin B6 was supplied to 10-times diluted vinasse; under the optimal condition, a decrease of chemical oxygen demand (COD) from 4,635 to 323 mg/L in vinasse was achieved in 5 days. The reduction of vinasse COD after the optimization of nutrient condition in this study is more significant than those reported previously.

scholarly journals Optimization of Crude Oil and PAHs Degradation by Stenotrophomonas rhizophila KX082814 Strain through Response Surface Methodology Using Box-Behnken Design

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Praveen Kumar Siddalingappa Virupakshappa ◽  
Manjunatha Bukkambudhi Krishnaswamy ◽  
Gaurav Mishra ◽  
Mohammed Ameenuddin Mehkri
Keyword(s):  
Response Surface Methodology ◽  
Crude Oil ◽  
Response Surface ◽  
Oxygen Demand ◽  
Inoculum Size ◽  
Coefficient Of Determination ◽  
Oil Removal ◽  
Box Behnken Design ◽  
Stenotrophomonas Rhizophila ◽  
Crude Oil Removal

The present paper describes the process optimization study for crude oil degradation which is a continuation of our earlier work on hydrocarbon degradation study of the isolate Stenotrophomonas rhizophila (PM-1) with GenBank accession number KX082814. Response Surface Methodology with Box-Behnken Design was used to optimize the process wherein temperature, pH, salinity, and inoculum size (at three levels) were used as independent variables and Total Petroleum Hydrocarbon, Biological Oxygen Demand, and Chemical Oxygen Demand of crude oil and PAHs as dependent variables (response). The statistical analysis, via ANOVA, showed coefficient of determination R2 as 0.7678 with statistically significant P value 0.0163 fitting in second-order quadratic regression model for crude oil removal. The predicted optimum parameters, namely, temperature, pH, salinity, and inoculum size, were found to be 32.5°C, 9, 12.5, and 12.5 mL, respectively. At this optimum condition, the observed and predicted PAHs and crude oil removal were found to be 71.82% and 79.53% in validation experiments, respectively. The % TPH results correlate with GC/MS studies, BOD, COD, and TPC. The validation of numerical optimization was done through GC/MS studies and   % removal of crude oil.

scholarly journals Modelization of Nutrient Removal Processes at a Large WWTP Using a Modified ASM2d Model

2018 ◽  
Vol 15 (12) ◽  
pp. 2817 ◽  
Author(s):  
Jakub Drewnowski ◽  
Jacek Makinia ◽  
Lukasz Kopec ◽  
Francisco-Jesus Fernandez-Morales
Keyword(s):  
Nutrient Removal ◽  
Carbon Sources ◽  
Oxygen Demand ◽  
Full Scale ◽  
Biological Nutrient Removal ◽  
Organic Substrates ◽  
Modified Model ◽  
Rate Limiting Step ◽  
Uptake Rates ◽  
Rate Limiting

The biodegradation of particulate substrates starts by a hydrolytic stage. Hydrolysis is a slow reaction and usually becomes the rate limiting step of the organic substrates biodegradation. The objective of this work was to evaluate a novel hydrolysis concept based on a modification of the activated sludge model (ASM2d) and to compare it with the original ASM2d model. The hydrolysis concept was developed in order to accurately predict the use of internal carbon sources in enhanced biological nutrient removal (BNR) processes at a full scale facility located in northern Poland. Both hydrolysis concepts were compared based on the accuracy of their predictions for the main processes taking place at a full-scale facility. From the comparison, it was observed that the modified ASM2d model presented similar predictions to those of the original ASM2d model on the behavior of chemical oxygen demand (COD), NH4-N, NO3-N, and PO4-P. However, the modified model proposed in this work yield better predictions of the oxygen uptake rate (OUR) (up to 5.6 and 5.7%) as well as in the phosphate release and uptake rates.

Physico-chemical monitoring of biodegradation of furfural from oil industry effluents from the Arzew refinery (Oran) Algeria

2022 ◽  
Vol 11 (6) ◽  
pp. 667-675
Author(s):  
Amina-Afaf MOUFFAK
Keyword(s):  
Biological Treatment ◽  
Oxygen Demand ◽  
Oil Industry ◽  
Petroleum Products ◽  
Wash Water ◽  
Treatment Level ◽  
Chemical Monitoring ◽  
Pollution Problem ◽  
Physico Chemical ◽  
Direct Discharge

Furfural is one of the petroleum products posing a potential danger to the environment and human health. However, the decontamination of these pollutants released into the environment is primarily governed by biodegra-dation processes. This study is based on biodegradation kinetics at increasing concentrations of furfural by natural mixed culture in order to assess the potential of this process in the elimination of furfural from petrochemical effluents from the ARZEW refinery. This biodegradation was measured through physicochemical parameters such as pH, electrical conductivity, con-centration of hydrocarbons, the chemical oxygen demand (COD), biochemi-cal oxygen demand (BOD5) and the concentration of furfural. The results obtained show at a concentration of 250ppm of injected furfural: a decrease in pH 4.9 and an increase in other parameters (conductivity 3450 μS.cm-1, HC 102 mg / l; furfural 210 ppm, COD 327mg / l, BOD5 98mgO2 / l. The study findings indicated that the injection of these effluents with concentrations greater than 180 ppm leads to values of pH, EC, HC, Furfural, COD, BOD5 which do not comply with direct discharge standards and disrupt biological treatment. The high levels of furfural not only cause a pollution problem but can also disrupt the functioning of bacteria at the biological treatment level. Therefore, dilution with the filtration wash water before switching to biologi-cal treatment is recommended in order to reduce the concentrations below 180 ppm.

Optimization of the condition for adsorption of gallic acid by Aspergillus oryzae mycelia using Box-Behnken design

2014 ◽  
Vol 22 (2) ◽  
pp. 1085-1094 ◽  
Author(s):  
Zhicai Zhang ◽  
Qiaoxia Pang ◽  
Min Li ◽  
Huihua Zheng ◽  
Hui Chen ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Aspergillus Oryzae ◽  
Box Behnken Design

scholarly journals The Optimization of Frying Process of Tumpi-tumpi from Milkfish used Response Surface Methodology

2018 ◽  
Vol 20 (3) ◽  
pp. 432 ◽  
Author(s):  
Syahrul Syahrul ◽  
Rizal Syarief ◽  
Joko Hermanianto ◽  
Budi Nurtama
Keyword(s):  
Quality Parameters ◽  
Raw Material ◽  
Frying Temperature ◽  
Frying Time ◽  
Coating Materials ◽  
Box Behnken Design ◽  
Design Expert ◽  
Frying Process ◽  
Optimal Processing ◽  
L Value

Tumpi-tumpi is a traditional food from South and West Sulawesi (ethnic Bugis-Makassar) using fish<br />as raw material. The quality parameters of tumpi-tumpi, especially in terms of oil absorption, color and<br />texture were affected by the condition of the frying process (including the percentage of coating materials,<br />temperature and frying time). This study was aimed to assess the production technology of the tumpi-tumpi<br />through frying process optimization of deep fat frying using Design Expert 7.0® software with Response<br />Surface Methodology (RSM) Box-Behnken Design producing an optimal response. The results showed that<br />the response of color (L value, ohue) and texture (hardness, cohesiveness) of tumpi-tumpi were significantly<br />influenced by the percentage of coating materials as well as frying temperature and time. The optimization<br />using software Design Expert 7.0® with RSM Box-Behnken produced the optimal processing formula of<br />1.5% coating materials, 150oC frying temperature and 30 s frying time produced the tumpi-tumpi with<br />properties as follows: 62.25 of L (lightness) value indicating a bit light, 78.73 of ohue indicating yellow red<br />color, 15407.60 gs hardness reflecting moderat hardness and 0.51 g/g cohesiveness impressing slight chewy.<br /><br />

scholarly journals Optimization of electrocoagulation operating parameters for COD removal from olive mill wastewater: application of Box-Behnken design

2019 ◽  
Vol 9 (3) ◽  
pp. 212-221
Author(s):  
Fatima Erraib ◽  
Khalid El Ass
Keyword(s):  
Response Surface ◽  
Chloride Concentration ◽  
Oxygen Demand ◽  
Olive Mill Wastewater ◽  
Operating Conditions ◽  
Cod Removal ◽  
Coefficient Of Determination ◽  
Box Behnken Design ◽  
Initial Ph ◽  
Olive Mill

Box–Behnken response surface design was successfully employed to optimize and study the olive mill wastewater (OMW) treatment by electrocoagulation (EC) process. The influence of four decisive factors were modelled and optimized to increase the removal of chemical oxygen demand (COD). The Box–Behnken design (BBD) results were analyzed and the second-order polynomial model was developed using multiple regression analysis. The model developed from the experimental design was predictive and a good fit with the experimental data with a high coefficient of determination (R2 ) value (more than 0.98). The optimal operating conditions based on Derringer’s desired function methodology are found to be; initial pH of 4.4, a current density of 27.6 mA/cm2 , electrolysis time of 14.1 min, and chloride concentration of 3.2 g/L. Under these conditions, the predicted COD removal efficiency was found to be 67.14% with a desirability value of 0.94. These experimental results were confirmed by validation experiments and proved that Box–Behnken design and response surface methodology could efficiently be applied for modelling of COD removal from OMW.

Treatment of waste activated sludge together with agro-waste by anaerobic digestion: focus on effluent quality

2013 ◽  
Vol 69 (3) ◽  
pp. 525-531 ◽  
Author(s):  
C. Cavinato ◽  
C. Da Ros ◽  
P. Pavan ◽  
F. Cecchi ◽  
D. Bolzonella
Keyword(s):  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Biogas Production ◽  
Oxygen Demand ◽  
Pilot Scale ◽  
Point Of View ◽  
Waste Activated Sludge ◽  
Organic Substrates ◽  
Economic Point ◽  
Energetic Balance

Waste activated sludge production and management plays an important role in wastewater treatment plants (WWTPs), especially from an economic point of view. One possible approach is the anaerobic co-digestion of waste activated sludge with others organic substrates in mesophilic and thermophilic conditions in order to exploit the spare volume of existing reactors, recover energy from biogas production, and obtain a fertilizer as final product. The anaerobic trials were carried out at pilot scale, applying two organic loading rates (2.8 and 4.5 kg chemical oxygen demand (COD)/(m3·d)) with a hydraulic retention time of 16 and 21 days. Among agro-wastes, wine lees were chosen because of their continuous availability throughout the year, and their high COD content (up to 200–300 g/l, 70% soluble, on average). The addition of wine lees to activated sludge determined a higher biogas production (best yield was 0.40 Nm3/kgCODfed) improving the energetic balance of the sludge line of the WWTP. The characterization of both substrates fed and digester effluents was carried out in terms of heavy metals; comparison with EC proposed limits showed that, due to high content of Cu in wine lees, the loading rate of this agro-waste should be limited to maintain good characteristics of final biosolids.

scholarly journals Forces modeling in a surface peripheral grinding process with the use of various design of experiment (DoE)

Mechanik ◽  
2018 ◽  
Vol 91 (10) ◽  
pp. 929-931 ◽  
Author(s):  
Witold Habrat ◽  
Marcin Żółkoś ◽  
Janusz Świder ◽  
Elżbieta Socha
Keyword(s):  
Standard Deviation ◽  
Coefficient Of Determination ◽  
Grinding Wheel ◽  
Grinding Process ◽  
Box Behnken Design ◽  
Residual Standard Deviation ◽  
Design Expert ◽  
Face Centered ◽  
Result Analysis ◽  
Central Composite

The paper presents forces modeling with the use of DoE models, such as (Box-Wilson) central composite design in face centered variant (CCF) and Box-Behnken design in a surface peripheral grinding process of 100Cr6 steel with M3X60K5VE01-35 grinding wheel. Experiment design and result analysis were done with the use of Design-Expert software. Force models, obtained with application of selected designs of experiment, were compared on the basis of the coefficient of determination, and values of residual standard deviation.

scholarly journals POTENSI EKSTRAK POLISAKARIDA LARUT AIR (PLA) DARI BIJI ASAM (Tamarindus indica L) SEBAGAI KONTROL BERAT BADAN DAN GLUKOSA DARAH

2021 ◽  
Vol 9 (2) ◽  
pp. 121-128
Author(s):  
Harijono Harijono ◽  
◽  
Aulia Azizah Syamsuri ◽  
Kiki Fibrianto ◽  
Siti Narsito Wulan ◽  
...  
Keyword(s):  
Software Design ◽  
Tamarindus Indica ◽  
Box Behnken Design ◽  
Design Expert

Biji asam jawa (Tamarindus indica L) merupakan salah satu potensi lokal Indonesia khususnya daerah Nusa Tenggara. Ekstrak polisakarida larut air (PLA) biji asam berperan sebagai sumber serat yang dapat mengendalikan berat badan dan glukosa darah. Tujuan penelitian yaitu untuk mengoptimasi ekstraksi PLA biji asam yang memiliki rendemen dan tingkat kelarutan yang paling baik sebagai kontrol berat badan dan glukosa darah. Proses optimasi menggunakan metode Box Behnken Design (BBD) dengan bantuan software Design Expert 10.0. Hasil optimasi yang diperoleh diverifikasi dan dilakukan pengujian potensinya terhadap berat badan dan glukosa darah. Hasil menunjukkan bahwa pemberian ekstrak PLA biji asam pada pakan tikus diet tinggi lemak selama 40 hari memiliki pengaruh signifikan terhadap kelompok kontrol negatif dan kontrol positif pada kadar glukosa darah tikus. Sehingga dapat disimpulkan pemberian ekstrak kasar PLA biji asam dapat mengendalikan kadar glukosa tikus diet tinggi lemak.

scholarly journals Aplikasi Metode Advance Oxidation Process (AOP) Fenton pada Pengolahan Limbah Cair Pabrik Kelapa Sawit

2016 ◽  
Vol 11 (1) ◽  
pp. 1 ◽  
Author(s):  
Ruka Yulia ◽  
Hesti Meilina ◽  
Adisalamun Adisalamun ◽  
Darmadi Darmadi
Keyword(s):  
Response Surface Methodology ◽  
Chemical Oxygen Demand ◽  
Response Surface ◽  
Oxidation Process ◽  
Oxygen Demand ◽  
Total Suspended Solid ◽  
Suspended Solid ◽  
Box Behnken Design ◽  
Advance Oxidation Process

Penelitian ini bertujuan untuk mengetahui kemampuan proses Fenton dalam menurunkan kadar chemical oxygen demand (COD) dan kadar total suspended solid (TSS) dari limbah cair pabrik kelapa sawit (PKS) dan menentukan kondisi optimum dari parameter yang digunakan dengan Response Surface Methodology menurut Box- Behnken design. Sampel diambil pada keluaran pertama kolam anaerobik ketiga dari instalasi pengolahan limbah cair kelapa sawit yang mengandung nilai COD berkisar antara 8.000 hingga 12.000 ppm. Pada penelitian ini, dilakukan pengujian pada berbagai pH, konsentrasi FeSO4.7H2O dan konsentrasi hidrogen peroksida. Hasil  penelitian menunjukkan bahwa kemampuan proses AOP dengan metode Fenton dapat menurunkan konsentrasi COD dan TSS masing-masing adalah 70,7704% dan 88,3897% pada konsentrasi FeSO4.7H2O 3703,52 ppm, konsentrasi H2O2 5586,43 ppm, dan pH 3.

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