Number of residual thermotolerant coliforms on plants and in soil when using reclaimed domestic wastewater for irrigation

2013 ◽  
Vol 67 (2) ◽  
pp. 380-386
Author(s):  
Sasirot Khamkure ◽  
Edmundo Peña Cervantes ◽  
Alejandro Zermeño González ◽  
Rubén López Cervantes ◽  
Prócoro Gamero Melo ◽  
...  

The reclamation of domestic wastewater for irrigation is one alternative approach to solve the water scarcity crisis, but it is essential to control the microbiological quality of wastewater used for irrigation. The removal of thermotolerant coliforms, also known as faecal coliforms (FC), from treated domestic wastewater by intermittent media infiltration (IMI) in column was studied. The columns were filled with natural filter media (soil, soil/charcoal and zinc-modified zeolite, Zeo-Zn), and wastewater, IMI-treated wastewater and disinfected wastewater were compared. The numbers of residual FC on Swiss chard (Beta vulgaris) and in agricultural soil were determined over a 4-month period. The column using Zeo-Zn had a higher FC removal efficiency (2.98 log) than columns with other filter media and disinfection (1.87–2.57 log) due to the bactericidal properties of Zn2+. The treatment of wastewater using Zeo-Zn and disinfection both decreased the accumulation of FC on plants and in soil to approximately 1–20 MPN/g dry matter. IMI-treated wastewater using the column with Zeo-Zn was suitable for unrestricted agricultural use, complied with Mexican regulations (as did disinfected wastewater) and had a low risk of FC contamination of plants and soil.

2002 ◽  
Vol 45 (1) ◽  
pp. 127-132 ◽  
Author(s):  
O.R. Vélez ◽  
G.E. Fasciolo ◽  
A.V. Bertranou

Arid areas call for imaginative water management solutions to avoid the dangers of water shortages. Growing demands of water for domestic and industrial uses decrease the availability of water for agriculture. It therefore becomes necessary to set up a policy for the use of domestic effluents. For the province of Mendoza, Argentina, with 1,500,000 inhabitants, a master plan was designed as of 1991 for the treatment of domestic effluents and subsequent disposal for irrigation. The guidelines set up by WHO for the use of wastewater in agricultural applications were taken into consideration. At present, the Province of Mendoza has available projects which are either complete, in execution or in the bidding process, entailing secondary treatment capacity with reuse of 320,000 cubic metres/day and an estimated possible irrigation area of 10,000 hectares. With this infrastructure, some strategic lines of action are recommended to establish a policy for the agricultural use of wastewater: (a) to program the use of treated wastewater to avoid discharges to irrigation flows; (b) to develop an institutional scheme for the efficient and safe use of these waters; and (c) develop scientific and technologic know-how to accompany the updated policies.


2018 ◽  
Vol 11 (1) ◽  
pp. 220
Author(s):  
J. de F. Xavier ◽  
C. A. V. de Azevedo ◽  
M. R. de Q. A. Azevedo ◽  
V. L. A. Lima ◽  
J. Dantas Neto ◽  
...  

The study aimed to determine the microbiological quality for coliform in three lettuce cultivars grown hydroponically with optimized solutions. The study was conducted in a protected environment and driven in a hydroponic system adopting the technique of laminar flow nutrientes-NFT, on the premises of the State University of Paraíba-Campus II in Lagoa Seca, PB having the following coordinates: 7°10′15″S; 35°51′14″W. The experimental design was a randomized block with a split-plot treatment in 7 × 3 factorial, with three replicates whose factors were 7 hydroponic solutions with three lettuce cultivars. The optimized solutions were formulated taking as reference the nutrient solution Furlani. The plot was constituted by nutrient solution (S). S1 = Furlani solution; S2 = domestic wastewater; S3 = optimized domestic wastewater; S4 = well water; S5 = optimized well water; S6 = wastewater from the UASB reactor and S7 = optimized wastewater from the UASB reactor and as for the subplot the three lettuce cultivars (Thais, Vanda and Veronica). In none of the samples of the three lettuce cultivars in the first experiment did the microbiological quality increase at 45°C, by the Most Probable Number (MPN) table, when the tube combination was 0-0-0, that is, without growth in none of the tubes, it is assumed that the MPN is less than 1.8 μg. ll samples were considered acceptable for the microbiological standard of thermotolerant coliforms at 45 °C/g. The leaf chlorophyll content is positively correlated with the N content in the plant.


2010 ◽  
Vol 56 (2) ◽  
pp. 188-194 ◽  
Author(s):  
Annie Locas ◽  
Veronica Martinez ◽  
Pierre Payment

Aerated lagoons offer a low-cost and simple approach to treating domestic wastewater in small municipalities. The objective of the current study was to evaluate, for each cell in the lagoons, the removal of indicator microorganisms and human enteric viruses under warm (summer) and cold (early spring) conditions. The two sites are located in southwest Quebec, Canada. Samples were assayed for thermotolerant coliforms, enterococci, Clostridium perfringens , somatic and male-specific coliphages, and culturable human enteric viruses (HEV). The results show higher removal under warm ambient conditions for all microorganisms. Thermotolerant coliforms and enterococci were removed to a greater extent than C. perfringens and HEV. HEV removal was only observed in warm ambient conditions. The removal of coliphages was different from the observed removal of HEV. The use of coliphages as surrogates for HEV has been proposed, but this does not seem appropriate for aerated lagoons, as the removal of coliphages overestimates the removal of HEV. Given the low observed removal of HEV during this study, the effluents remain a significant source of pathogens that can affect drinking water treatment plants drawing their raw water from receiving streams. Ultraviolet disinfection of treated wastewater effluent is a possible solution.


1995 ◽  
Vol 31 (5-6) ◽  
pp. 19-26 ◽  
Author(s):  
G. J. Medema ◽  
I. A. van Asperen ◽  
J. M. Klokman-Houweling ◽  
A. Nooitgedagt ◽  
M. J. W. van de Laar ◽  
...  

This pilot study was carried out to determine the relationship between microbiological water quality parameters and the occurrence of health complaints among triathletes. Data were collected at an Olympic distance triathlon (n=314) and a run-bike-run (n=81; controls for exposure to fresh water). At the time of the triathlon, the concentrations of Escherichia coli , thermotolerant coliforms, faecal streptococci, entero- and reoviruses, F-specific RNA phages, Salmonella, Campylobacter, Aeromonas, Plesiomonas shigelloides, Pseudomonas aeruginosa and Staphylococcus aureus were examined over the swimming course. Information on the occurrence of health complaints during the competition and in the week thereafter was collected through a written questionnaire. The results show that triathletes and run-bike-runners are comparable with respect to factors other than water exposure (age, sex, training history, physical stress, lower intestinal health complaints during the competition) that may influence the occurrence of health complaints in the week after the competition. Triathletes and run-bike-runners reported gastro-intestinal (7.7% vs 2.5%), respiratory (5.5% vs 3.7%), skin/mucosal (2.6% vs 1.2%), general (3.5% vs 1.2%) and total symptoms (14.8% vs 7.4%) in the week after the event. The health risks for triathletes for all symptom groups are not significantly higher than for run-bike-runners. The geometric mean concentration of faecal indicator bacteria is relatively low: E. coli 170/100 ml; faecal streptococci 13/100 ml, enteroviruses were present at concentrations of 0.1/l. The group of triathletes was homogeneusly and relatively intensely exposed to water; they all swam in the same body of water at the same time and 75% reported to have swallowed freshwater. It was concluded that this study design is suitable to study the relationship between health complaints and microbiological water quality. In the summers of 1993 and 1994, a study will be carried out concerning several run-bike-runs and triathlons in freshwaters of different quality.


1997 ◽  
Vol 35 (11-12) ◽  
pp. 157-163 ◽  
Author(s):  
G. J. Medema ◽  
I. A. van Asperen ◽  
A. H. Havelaar

As part of a prospective cohort study among triathletes to determine a relationship between the microbiological quality of fresh bathing water and the risk of acquiring an intestinal infection, the exposure of the triathletes to microbiological contaminants was assessed. Waters were collected at seven triathlons (swimming course 1–1.5km) held in the summer of 1993 and 1994 to have a range of water qualities. All were influenced by sewage effluents, most also by agricultural run-off. Samples were collected several weeks before the event to establish a sampling programme (1993) and during the actual exposure of the triathletes (1993 and 1994) and examined for thermotolerant coliforms alone (samples preceding the event) and for E. coli, faecal enterococci, Staphylococcus aureus, F-specific RNAphages, enteroviruses (1993 and 1994) and for thermophilic Campylobacter, Salmonella, Aeromonas, Plesiomonas shigelloides and Pseudomonas aeruginosa (1993). The samples taken in the weeks before the exposure showed significant differences in thermotolerant coliform concentration between locations, depths and times. Also during swimmer exposure, significant differences occurred in microorganism levels at the different sampling points over the swimming course. As the triathletes swam as a group, they were exposed to approximately the same water at the same time. The geometric mean concentration was used to characterise each site. In the epidemiological study, the risk of an intestinal infection correlated with the concentration of thermotolerant coliforms and E. coli but not with the other parameters. The geometric mean concentration of thermotolerant coliforms at the triathlons ranged from 11–330/100mL and 54–1,200/100mL E. coli. Ranking of the seven sites by faecal pollution level, based on the geometric mean concentration of a faecal indicator, resulted in a different ranking for each indicator. At the fresh water sites studied, only the ratio between the geometric mean density of E. coli and thermotolerant coliforms was constant. The ratio between the other parameters related to faecal pollution (faecal enterococci, F-specific RNA phages, enteroviruses) varied considerably. Water quality standards relating to faecal pollution can only be based on parameters that show a significant correlation with risk of intestinal illness.


2021 ◽  
Vol 11 (4) ◽  
pp. 1889 ◽  
Author(s):  
Agnieszka Micek ◽  
Krzysztof Jóźwiakowski ◽  
Michał Marzec ◽  
Agnieszka Listosz ◽  
Tadeusz Grabowski

The results of research on the efficiency and technological reliability of domestic wastewater purification in two household wastewater treatment plants (WWTPs) with activated sludge are presented in this paper. The studied facilities were located in the territory of the Roztocze National Park (Poland). The mean wastewater flow rate in the WWTPs was 1.0 and 1.6 m3/day. In 2017–2019, 20 series of analyses were done, and 40 wastewater samples were taken. On the basis of the received results, the efficiency of basic pollutant removal was determined. The technological reliability of the tested facilities was specified using the Weibull method. The average removal efficiencies for the biochemical oxygen demand in 5 days (BOD5) and chemical oxygen demand (COD) were 66–83% and 62–65%, respectively. Much lower effects were obtained for total suspended solids (TSS) and amounted to 17–48%, while the efficiency of total phosphorus (TP) and total nitrogen (TN) removal did not exceed 34%. The analyzed systems were characterized by the reliability of TSS, BOD5, and COD removal at the level of 76–96%. However, the reliability of TN and TP elimination was less than 5%. Thus, in the case of biogenic compounds, the analyzed systems did not guarantee that the quality of treated wastewater would meet the requirements of the Polish law during any period of operation. This disqualifies the discussed technological solution in terms of its wide application in protected areas and near lakes, where the requirements for nitrogen and phosphorus removal are high.


2009 ◽  
Vol 39 (6) ◽  
pp. 1836-1841 ◽  
Author(s):  
Thaís Mioto Martineli ◽  
Oswaldo Durival Rossi Junior ◽  
Natacha Deboni Cereser ◽  
Marita Vedovelli Cardozo ◽  
Cristianne Lino Fontoura ◽  
...  

The consumption of lamb meat in Brazil has increased in the last years but little information about the microbiological quality of this product is available. To evaluate the hygienic-sanitary conditions of lamb carcasses, the quantification of microorganism populations indicators (mesophiles and psychrotrophs; total and thermotolerant coliforms; Escherichia coli; moulds and yeasts) and the pathogenic microorganisms indentification (Salmonella sp. and Listeria spp.) were performed. A total of 60 lamb carcasses were sampled from one abattoir in São Paulo. Swab samples were collected from three points (forequarter, back and hindquarter) on the muscle surface after carcasses final washing. Statistical analysis consisted of descriptive evaluation of the results whose counts were grouped by intervals of microorganism populations. Counts ranged from 1.0 x 10¹ to 8.0 x 10(4) colony-forming unit cm-2 (CFU cm-2) for mesophiles; 1.0 x 10(0) to 4.4 x 10(4)CFU cm-2 for psychrotrophs; < 1.0 x 10(0) to 4.4 x 10(4)CFU cm-2 for moulds and yeasts; < 0.3 to > 32.0 most probable number/cm² (MPN cm-2) for total and thermotolerant coliforms and Escherichia coli. Salmonella sp. and Listeria spp. were not found in any of the carcasses. Most carcasses presented low counts for all microorganisms. Overall results may be explained by the small size of the industry where the study was taken. Results suggest that good microbiological quality lamb meat is possible to be obtained, but improvement in hygienic-sanitary conditions is still required.


2020 ◽  
Vol 5 (1) ◽  
pp. 27-35
Author(s):  
Fivi Elvira Sirajuddin ◽  
Muhammad Fadly Saleh

This experimental study aims to determine the most optimal biofiltration media composition for domestic wastewater treatment using 4 upflow biofilter reactors with a combining pumice and coconut shell charcoal as an media filter. ,The combination ratio of media filter is UAF 1 = 1: 0, UAF 2 = 2: 1, UAF 3 = 1: 2, and UAF 4 = 1 : 1 with an overall filter media thickness of 60 cm. Sampling time is done every 2 days for 16 day were taken at the four experimental reactors to be tested for each parameter of pH, COD, nitrate and ammonia. The method of analysis carried out after obtaining data from testing in the laboratory, that is descriptively related to variations in filter media, and the residence time of wastewater in the reactor. The results showed that the reactor with the best processing in normalizing pH was UAF 1. While the best filter media composition in COD and nitrate removal was UAF 3 reactor with 77.78% and 87.17% removal results. Ammonia removal occurred throughout the experimental reactor with a result <-0.05 mg / l was considered quite good and indicated the decomposition process was successful. Penelitian ini dilakukan untuk menentukan komposisi media biofiltrasi yang paling optimal untuk pengolahan air limbah domestik dengan menggunakan 4 reaktor upflow biofilter dengan mengkombinasikan batu apung dan arang tempurung kelapa sebagai media filter. Rasio dari kombinasi media filter tersebut adalah UAF 1 = 1:0, UAF 2 = 2:1, UAF 3=1:2, dan UAF 4=1:1 dengan ketebalan media filter secara keseluruhan 60 cm. Waktu pengambilan sampel dilakukan setiap 2 hari selama 16 hari. Pengambilan sampel dilakukan pada keempat reaktor percobaan untuk diujikan masing-masing parameter pH, COD, nitrat dan amoniak. Metode analisa yang dilakukan setelah mendapatkan data dari pengujian di laboratorium, yaitu secara deskriptif terkait dengan variasi media filter, dan waktu tinggal air limbah dalam reaktor. Hasil penelitian menunjukkan reaktor yang paling baik pengolahannya dalam menormalkan pH adalah UAF 1. Sementara komposisi media filter yang paling baik dalam penyisihan COD dan nitrat adalah reaktor UAF 3 dengan hasil penyisihan sebesar 77,78% dam 87,17%. Penyisihan amoniak terjadi di seluruh reaktor percobaan dengan hasil <-0,05 mg/l dinilai cukup baik dan mengindikasikan proses penguraian polutan berjalan dengan baik.


AMBIO ◽  
2020 ◽  
Vol 50 (2) ◽  
pp. 413-424 ◽  
Author(s):  
Michał Preisner ◽  
Elena Neverova-Dziopak ◽  
Zbigniew Kowalewski

AbstractMitigation of eutrophication, intensified by excessive nutrient load discharge in wastewaters regulated by restrictive legal requirements, remains one of today’s most important global problems. Despite implementation of the Water Framework Directive, the Urban Wastewater Directive and the HELCOM recommendations, the actual condition of surface water is still not satisfactory. In response to the above, the study presents an alternative approach for surface water protection against eutrophication based on the selection of appropriate nutrient removal technologies. An activated sludge model simulation was used to enable the identification of environmentally justified nutrient removal systems with lowest eutrophication potential of treated wastewater conditioned by bioavailable nutrient forms content. Based on the outcome of the study, the 3-stage Bardenpho system was identified as the most efficient for bioavailable phosphorus removal, while the Johannesburg system proved to have the highest efficiency for bioavailable nitrogen removal. The proposed eutrophication mitigation approach underlines the need for a reconsideration of current legal regulations which ignore nutrient bioavailability and key eutrophication limiting factors.


2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Solveig L. Johannesdottir ◽  
Biljana Macura ◽  
Jennifer McConville ◽  
Dag Lorick ◽  
Neal R. Haddaway ◽  
...  

Abstract Background Eutrophication of the Baltic Sea, and many other water bodies, is partly the result of point-source emissions of nutrients and carbon from wastewater. At the same time, nitrogen and phosphorus planetary boundaries have been breached. There is a need for more efficient resource management, including the recovery and reuse of nutrients and carbon in waste. The aim of this paper is to collate evidence on ecotechnologies intended for use in the wastewater sector globally to facilitate the recovery or reuse of carbon and/or nutrients. Methods Searches were performed on literature published between 2013 and 2017 and in 5 bibliographic databases, 1 search engine, and 38 specialist websites. Database searches were performed in English. Searches in specialist websites were also performed in Finnish, Polish and Swedish. There was no geographical limitation. Screening was conducted at title and abstract level, and on full texts. Apart from bibliographical information, we extracted information on ecotechnology type, intervention, details of the recovery or reuse, the type of wastewater stream to which the ecotechnology is applied, the study location, type and design. Prior to screening and coding, we conducted consistency checks amongst reviewers. We generated a searchable database of coded studies. Findings were synthesised narratively and visualised in a geographical information system (i.e. an evidence atlas). We identified a series of knowledge gaps and clusters that warrant further research. Results The search resulted in 4024 records, out of which 413 articles were retained after the screening process. In addition, 35 pre-screened studies from the specialist website searches were added. Together, these 448 articles contained 474 individual studies of 28 types of ecotechnologies. A combination of ecotechnologies (16.7%), followed by microalgae cultivation (14.1%) were the most frequent ecotechnologies in the evidence base. Ecotechnologies for recovery composed 72.6% of the evidence base. The most common wastewater streams for recovery were mixed wastewater and sludge (73.8%). There was a relative lack of studies on recovery from source-separated wastewater. The most common type of recovery was energy (27.3%), followed by simultaneous recovery of nitrogen and phosphorus (22.1%). Reuse of recovered substances was described in 22.8% of the studies. The most common type of reuse was of nitrogen and phosphorus (57.4%), followed by joint reuse of organic carbon, nitrogen and phosphorus (35.2%). Reuse ecotechnologies were mostly focused on the use of wastewater for irrigation or reuse of biosolids, and not on the nutrients that had been extracted through e.g. precipitation of struvite. In 22 studies both recovery and reuse were described. In total, 60 different study countries were reported in the evidence base, and the most common study location was China. Conclusions We found substantial evidence for the recovery and reuse of nutrients and carbon from wastewater sources. The relative abundance of studies where substances are recovered compared to studies where they are reused, suggests a knowledge gap on reuse of recovered nutrients and carbon. The majority of studies on reuse were on irrigation with treated wastewater or reuse of biosolids, and not on reuse of extracted nutrients such as struvite.


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