Biodegradation of o-nitrophenol by aerobic granules with glucose as co-substrate

2012 ◽  
Vol 65 (12) ◽  
pp. 2132-2139 ◽  
Author(s):  
Farrukh Basheer ◽  
M. H. Isa ◽  
I. H. Farooqi
Keyword(s):  
Activated Sludge ◽  
Kinetic Parameters ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Aerobic Granules ◽  
Haldane Model

Aerobic granules to treat wastewater containing o-nitrophenol were successfully developed in a sequencing batch reactor (SBR) using activated sludge as inoculum. Stable aerobic granules were obtained with a clearly defined shape and diameters ranging from 2 to 6 mm after 122 days of operation. The integrity coefficient (IC) and granules density was found to be 98% and 1,054 kg m−3 respectively. After development of aerobic granules, o-nitrophenols were successfully degraded to an efficiency of 78% at a concentration of 70 mg L−1. GC-MS study revealed that the biodegradation of o-nitrophenol occured via catechol via nitrobenzene pathway. Specific o-nitrophenol biodegradation rates followed the Haldane model and the associated kinetic parameters were found as follows: Vmax = 3.96 g o-nitrophenol g−1VSS−1d−1, Ks = 198.12 mg L−1, and Ki = 31.16 mg L−1. The aerobic granules proved to be a feasible and effective way to degrade o-nitrophenol containing wastewater.

scholarly journals Development of aerobic granules in sequencing batch reactor with p-nitrophenol as sole carbon source

2012 ◽  
Vol 2 (1) ◽  
pp. 22-32 ◽  
Author(s):  
Farrukh Basheer ◽  
I. H. Farooqi
Keyword(s):  
Removal Efficiency ◽  
Degradation Rate ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Substrate Inhibition ◽  
Gas Chromatography Mass Spectrometry ◽  
Aerobic Granules ◽  
Compact Structure ◽  
Inhibitory Compounds ◽  
Haldane Model

The present study was aimed at the development of aerobic granules in sequencing batch reactor (SBR) for the biodegradation of p-nitrophenol (PNP). The reactor was started with 50 mg L−1 of PNP. Aerobic granules first appeared within 1 month of the start up of the reactor. The granules were large and strong and had a compact structure. The diameter of stable granules on day 200 was in the range of 2–3 mm. The integrity coefficient and granule density was found to be 98% and 1,031 kg m−3 respectively. The settling velocity of granules was found to be in the range of 3 × 10−2 to 4 × 10−2m s−1. The aerobic granules were able to degrade PNP up to 700 mg L−1 at a removal efficiency of 87%. Gas chromatography–mass spectrometry studies confirmed that the biodegradation of PNP occurred by an initial oxygenase attack that resulted in the release of nitrite and the accumulation of hydroquinone. The specific PNP degradation rate in aerobic granules followed the Haldane model for substrate inhibition. A high specific PNP degradation rate up to 0.872 g PNP g−1 VSS−1 d−1 was sustained up to PNP concentration of 200 mg L−1. Higher removal efficiency, good settling characteristics of aerobic granules, makes a SBR suitable for enhancing the microorganism potential for biodegradation of inhibitory compounds.

Biodegradation of Congo Red by Aerobic Granules in a Sequencing Batch Reactor

2014 ◽  
Vol 955-959 ◽  
pp. 656-662
Author(s):  
Deng Yue Ma ◽  
Xin Hua Wang ◽  
Chao Song ◽  
Shu Guang Wang
Keyword(s):  
Activated Sludge ◽  
Organic Compounds ◽  
Congo Red ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Aerobic Granules ◽  
Biological Removal ◽  
Target Treatment

In this study, compact well-settling aerobic granules were developed in a sequencing batch reactor (SBR) for the biological removal of Congo red (CR) using activated sludge as inoculum. The granules had a clearly defined shape and appearance, and were capable of removing over 90% of CR and COD. The result in this research demonstrates that it is possible to use aerobic granules for CR biodegradation and broadens the benefits of using the SBR to target treatment of toxic and recalcitrant organic compounds.

A Technique to Determine Unsteady-State Inhibition Kinetics in the Activated Sludge Process

1989 ◽  
Vol 21 (6-7) ◽  
pp. 593-602 ◽  
Author(s):  
Andrew T. Watkin ◽  
W. Wesley Eckenfelder
Keyword(s):  
Activated Sludge ◽  
Kinetic Parameters ◽  
Curve Fitting ◽  
Glucose Utilization ◽  
Batch Reactor ◽  
Synthetic Data ◽  
Utilization Rate ◽  
Fitting Algorithm ◽  
Test Reactor ◽  
Two Parameter

A technique for rapidly determining Monod and inhibition kinetic parameters in activated sludge is evaluated. The method studied is known as the fed-batch reactor technique and requires approximately three hours to complete. The technique allows for a gradual build-up of substrate in the test reactor by introducing the substrate at a feed rate greater than the maximum substrate utilization rate. Both inhibitory and non-inhibitory substrate responses are modeled using a nonlinear numerical curve-fitting technique. The responses of both glucose and 2,4-dichlorophenol (DCP) are studied using activated sludges with various acclimation histories. Statistically different inhibition constants, KI, for DCP inhibition of glucose utilization were found for the various sludges studied. The curve-fitting algorithm was verified in its ability to accurately retrieve two kinetic parameters from synthetic data generated by superimposing normally distributed random error onto the two parameter numerical solution generated by the algorithm.

Enrichment and characterization of an anaerobic methyl ethyl ketoxime degrading culture from an anoxic/anaerobic/aerobic activated sludge sequencing batch reactor

1998 ◽  
Vol 37 (4-5) ◽  
pp. 95-98 ◽  
Author(s):  
Nancy G. Love ◽  
Mary E. Rust ◽  
Kathy C. Terlesky
Keyword(s):  
Energy Source ◽  
Activated Sludge ◽  
Electron Acceptor ◽  
Sequencing Batch Reactor ◽  
Enrichment Culture ◽  
Batch Reactor ◽  
Nitrification Inhibitor ◽  
Time Frame ◽  
Methyl Ethyl

An anaerobic enrichment culture was developed from an anoxic/anaerobic/aerobic activated sludge sequencing batch reactor using methyl ethyl ketoxime (MEKO), a potent nitrification inhibitor, as the sole carbon and energy source in the absence of molecular oxygen and nitrate. The enrichment culture was gradually fed decreasing amounts of biogenic organic compounds and increasing concentrations of MEKO over 23 days until the cultures metabolized the oxime as the sole carbon source; the cultures were maintained for an additional 41 days on MEKO alone. Turbidity stabilized at approximately 100 mg/l total suspended solids. Growth on selective media plates confirmed that the microorganisms were utilizing the MEKO as the sole carbon and energy source. The time frame required for growth indicated that the kinetics for MEKO degradation are slow. A batch test indicated that dissolved organic carbon decreased at a rate comparable to MEKO consumption, while sulfate was not consumed. The nature of the electron acceptor in anaerobic MEKO metabolism is unclear, but it is hypothesized that the MEKO is hydrolyzed intracellularly to form methyl ethyl ketone and hydroxylamine which serve as electron donor and electron acceptor, respectively.

Excess nitrogen accumulation in activated sludge in sequencing batch reactor with a single-stage oxic process

2009 ◽  
Vol 59 (3) ◽  
pp. 573-582 ◽  
Author(s):  
Xiao-ming Li ◽  
Dong-bo Wang ◽  
Qi Yang ◽  
Wei Zheng ◽  
Jian-bin Cao ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Nitrogen Removal ◽  
Sequencing Batch Reactor ◽  
Ph Value ◽  
Batch Reactor ◽  
Nitrogen Loss ◽  
Single Stage ◽  
Synthetic Wastewater ◽  
Nitrogen Accumulation ◽  
Excess Nitrogen

It was occasionally found that a significant nitrogen loss in solution under neutral pH value in a sequencing batch reactor with a single-stage oxic process using synthetic wastewater, and then further studies were to verify the phenomenon of nitrogen loss and to investigate the pathway of nitrogen removal. The result showed that good performance of nitrogen removal was obtained in system. 0–7.28 mg L−1 ammonia, 0.08–0.38 mg L−1 nitrite and 0.94–2.12 mg L−1 nitrate were determined in effluent, respectively, when 29.85–35.65 mg L−1 ammonia was feeding as the sole nitrogen source in influent. Furthermore, a substantial nitrogen loss in solution (95% of nitrogen influent) coupled with a little gaseous nitrogen increase in off-gas (7% of nitrogen influent) was determined during a typical aerobic phase. In addition, about 322 mg nitrogen accumulation (84% of nitrogen influent) was detected in activated sludge. Based on nitrogen mass balance calculation, the unaccounted nitrogen fraction and the ratio of nitrogen accumulation in sludge/nitrogen loss in solution were 14.6 mg (3.7% of nitrogen influent) and 0.89, respectively. The facts indicated that the essential pathway of nitrogen loss in solution in this study was excess nitrogen accumulation in activated sludge.

Advanced Treatment of Domestic Wastewater Using Sequencing Batch Reactor Activated Sludge Process

1993 ◽  
Vol 28 (10) ◽  
pp. 267-274 ◽  
Author(s):  
M. Imura ◽  
E. Suzuki ◽  
T. Kitao ◽  
S. Iwai
Keyword(s):  
Activated Sludge ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Domestic Wastewater ◽  
Experimental Period ◽  
Small Scale ◽  
Experimental Plant ◽  
Activated Sludge Process ◽  
Nitrogen And Phosphorus ◽  
Experimental Apparatus

In order to apply a sequencing batch reactor activated sludge process to small scale treatment facilities, various experiments were conducted by manufacturing an experimental apparatus made of a factory-produced FRP cylinder transverse tank (Ø 2,500mm). Results of the verification test conducted for one year by leading the wastewater discharged from apartment houses into the experimental apparatus were as follows. Excellent performance was achieved without any addition of carbon source, irrespective of the organic compound concentration and the temperature of raw wastewater. Organic substances, nitrogen and phosphorus were removed simultaneously. Due to the automated operation format, stable performance was obtained with only periodic maintenance. Though water depth of the experimental plant was shallow, effective sedimentation of activated sludge was continued during the experimental period. Regarding the aerobic and anaerobic process, nitrification and denitrification occurred smoothly.

scholarly journals Dynamic control of nutrient-removal from industrial wastewater in a sequencing batch reactor, using common and low-cost online sensors

2015 ◽  
Vol 73 (4) ◽  
pp. 740-745 ◽  
Author(s):  
Jan Dries
Keyword(s):  
Activated Sludge ◽  
Nutrient Removal ◽  
Industrial Wastewater ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Low Cost ◽  
Dynamic Control ◽  
Oxygen Demand ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential

On-line control of the biological treatment process is an innovative tool to cope with variable concentrations of chemical oxygen demand and nutrients in industrial wastewater. In the present study we implemented a simple dynamic control strategy for nutrient-removal in a sequencing batch reactor (SBR) treating variable tank truck cleaning wastewater. The control system was based on derived signals from two low-cost and robust sensors that are very common in activated sludge plants, i.e. oxidation reduction potential (ORP) and dissolved oxygen. The amount of wastewater fed during anoxic filling phases, and the number of filling phases in the SBR cycle, were determined by the appearance of the ‘nitrate knee’ in the profile of the ORP. The phase length of the subsequent aerobic phases was controlled by the oxygen uptake rate measured online in the reactor. As a result, the sludge loading rate (F/M ratio), the volume exchange rate and the SBR cycle length adapted dynamically to the activity of the activated sludge and the actual characteristics of the wastewater, without affecting the final effluent quality.

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