A metabolic network of a phosphate-accumulating organism provides new insights into enhanced biological phosphorous removal

2011 ◽  
Vol 64 (12) ◽  
pp. 2410-2416
Author(s):  
Sergio Bordel
Keyword(s):  
Metabolic Network ◽  
Process Design ◽  
Carbon Metabolism ◽  
Central Carbon Metabolism ◽  
Phosphorous Removal ◽  
Flux Balance ◽  
Central Carbon ◽  
Balance Analysis ◽  
Polyphosphate Accumulation ◽  
Accumulibacter Phosphatis

Here we present a metabolic network representing the central carbon metabolism as well as the synthesis of polyhydroxyalcanohates and the polyphosphate accumulation mechanisms of the bacterium Candidatus Accumulibacter phosphatis, which was previously identified from metagenomic studies in enhanced biological phosphorous removal sludges. The reconstructed metabolic network, together with flux balance analysis can be used to provide new insights into controversial aspects of the metabolism of phosphate-accumulating organisms and is also a tool that can be used in to help enhanced biological phosphorous removal (EBPR) process design and operation.

scholarly journals Entner–Doudoroff pathway and Non-OxPPP bypasses glycolysis and OxPPP in Ralstonia solanacearum

2020 ◽  
Author(s):  
Poonam Jyoti ◽  
Manu Shree ◽  
Chandrakant Joshi ◽  
Tulika Prakash ◽  
Suvendra Kumar Ray ◽  
...  
Keyword(s):  
Network Analysis ◽  
Metabolic Network ◽  
Ralstonia Solanacearum ◽  
Metabolic Flux ◽  
Pentose Phosphate ◽  
Flux Analysis ◽  
Flux Balance ◽  
Metabolic Network Analysis ◽  
Central Carbon ◽  
Balance Analysis

AbstractIn Ralstonia solanacearum, a devastating phytopathogen whose metabolism is poorly understood, we observed that Entner-Doudoroff (ED) pathway and NonOxidative pentose phosphate pathway (OxPPP) bypasses glycolysis and OxPPP under glucose oxidation. Evidences derived from 13C stable isotopes feeding and genome annotation based comparative metabolic network analysis supported the observations. Comparative metabolic network analysis derived from the currently available 53 annotated R. solanacearum strains also including the recently reported strain (F1C1), representing the four phylotypes confirmed the lack of key genes coding for phosphofructokinase (pfk-1) and phosphogluconate dehydrogenase (gnd) enzymes that are relevant for glycolysis and OxPPP respectively. R. solanacearum F1C1 cells fed with 13C Glucose (99%[1-13C]- or 99%[1,2-13C]- or 40%[13C6]-glucose) followed by GC-MS based labelling analysis of fragments from amino acids, glycerol and ribose provided clear evidence that rather than Glycolysis and OxPPP, ED pathway and NonOxPPP are the main routes sustaining metabolism in R. solanacearum. The 13C incorporation in the mass ions of alanine (m/z 260, m/z 232); valine (m/z 288, m/z 260), glycine (m/z 218), serine (m/z 390, m/z 362), histidine (m/z 440, m/z 412), tyrosine (m/z 466, m/z 438), phenylalanine (m/z 336, m/z 308), glycerol (m/z 377) and ribose (m/z 160) mapped the pathways supporting the observations. The outcomes help better defining the central carbon metabolic network of R. solanacearum that can be integrated with 13C metabolic flux analysis as well as flux balance analysis studies for defining the metabolic phenotypes.ImportanceUnderstanding the metabolic versatility of Ralstonia solanacearum is important as it regulates the tradeoff between virulence and metabolism (1, 2) in a wide range of plant hosts. Due to a lack of clear evidence until this work, several published research papers reported on potential roles of Glycolysis and Oxidative pentose phosphate pathways (OxPPP) in R. solanacearum (3, 4). This work provided evidence from 13C stable isotopes feeding and genome annotation based comparative metabolic network analysis that Entner-Doudoroff pathway and Non-OxPPP bypasses glycolysis and OxPPP during the oxidation of Glucose, one of the host xylem pool that serves as a potential carbon source (5). The outcomes help better defining the central carbon metabolic network of R. solanacearum that can be integrated with 13C metabolic flux analysis as well as flux balance analysis studies for defining the metabolic phenotypes. The study highlights the need to critically examine phytopathogens whose metabolism is poorly understood.

scholarly journals Metformin Targets Central Carbon Metabolism and Reveals Mitochondrial Requirements in Human Cancers

2016 ◽  
Vol 24 (5) ◽  
pp. 728-739 ◽  
Author(s):  
Xiaojing Liu ◽  
Iris L. Romero ◽  
Lacey M. Litchfield ◽  
Ernst Lengyel ◽  
Jason W. Locasale
Keyword(s):  
Carbon Metabolism ◽  
Central Carbon Metabolism ◽  
Human Cancers ◽  
Central Carbon

Trisomy 21 results in modest impacts on mitochondrial function and central carbon metabolism

2021 ◽  
Author(s):  
Colin C. Anderson ◽  
John O. Marentette ◽  
Kendra M. Prutton ◽  
Abhishek K. Rauniyar ◽  
Julie A. Reisz ◽  
...  
Keyword(s):  
Mitochondrial Function ◽  
Carbon Metabolism ◽  
Trisomy 21 ◽  
Central Carbon Metabolism ◽  
Central Carbon

Metabolic phenotyping of the Yersinia high-pathogenicity island that regulates central carbon metabolism

The Analyst ◽  
2015 ◽  
Vol 140 (10) ◽  
pp. 3356-3361 ◽  
Author(s):  
Leyu Yan ◽  
Wenna Nie ◽  
Haitao Lv
Keyword(s):  
Carbon Metabolism ◽  
Virulence Genes ◽  
Pathogenicity Island ◽  
Central Carbon Metabolism ◽  
Metabolic Phenotyping ◽  
High Pathogenicity ◽  
Central Carbon ◽  
Regulatory Effects

The regulatory effects of the HPI virulence genes on central carbon metabolism differentiate UPEC from non-UPEC.

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