Anaerobic degradation of palm oil mill effluent (POME)

2011 ◽  
Vol 64 (10) ◽  
pp. 2001-2008 ◽  
Author(s):  
W. Yoochatchaval ◽  
S. Kumakura ◽  
D. Tanikawa ◽  
T. Yamaguchi ◽  
M. F. M. Yunus ◽  
...  
Keyword(s):  
Palm Oil ◽  
Anaerobic Degradation ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Enrichment Culture ◽  
Palm Oil Mill Effluent ◽  
Enrichment Cultures ◽  
Degrading Bacteria ◽  
Thermophilic Conditions ◽  
Gradient Gel Electrophoresis ◽  
Palm Oil Mill

The biodegradation characteristics of palm oil mill effluent (POME) and the related microbial community were studied in both actual sequential anaerobic ponds in Malaysia and enrichment cultures. The significant degradation of the POME was observed in the second pond, in which the temperature was 35–37 °C. In this pond, biodegradation of major long chain fatty acids (LCFA), such as palmitic acid (C16:0) and oleic acid (C18:1), was also confirmed. The enrichment culture experiment was conducted with different feeding substrates, i.e. POME, C16:0 and C18:1, at 35 °C. Good recovery of methane indicated biodegradation of feeds in the POME and C16:0 enrichments. The methane production rate of the C18:1 enrichment was slower than other substrates and inhibition of methanogenesis was frequently observed. Denaturing gradient gel electrophoresis (DGGE) analyses indicated the existence of LCFA-degrading bacteria, such as the genus Syntrophus and Syntorophomonas, in all enrichment cultures operated at 35 °C. Anaerobic degradation of the POME under mesophilic conditions was stably processed as compared with thermophilic conditions.

scholarly journals Performance of Anaerobic Digestion of Acidified Palm Oil Mill Effluent under Various Organic Loading Rates and Temperatures

Water ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2432
Author(s):  
Muhammad Arif Fikri Hamzah ◽  
Peer Mohamed Abdul ◽  
Safa Senan Mahmod ◽  
Azratul Madihah Azahar ◽  
Jamaliah Md. Jahim
Keyword(s):  
Anaerobic Digestion ◽  
Palm Oil ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Oxygen Demand ◽  
Microbial Community Analysis ◽  
Palm Oil Mill Effluent ◽  
Methane Formation ◽  
Organic Loading ◽  
Loading Rates ◽  
Palm Oil Mill

This study compared the performance of thermophilic and mesophilic digesters of an anaerobic digestion system from palm oil mill effluent (POME), in which temperature is a key parameter that can greatly affect the performance of anaerobic digestion. The digesters were incubated at two distinct temperatures of 55 and 37 °C, and operated with varying organic loading rates (OLRs) of 2.4, 3.2, and 4.0 g COD/L.d by altering the chemical oxygen demand (COD) of acidified POME during feeding. The results indicated that the performance of anaerobic digestion increased as the OLR increased from 2.4 to 4.0 g COD/L.d. At the OLR of 4.0 g COD/L.d, the thermophilic condition showed the highest methane yield of 0.31 ± 0.01 L/g COD, accompanied by the highest COD removal and volatile solid reduction, which were found to be higher than the mesophilic condition. Microbial community analysis via denaturing gradient gel electrophoresis (DGGE) revealed that Methanothermobacter sp. emerges as the dominant microbe, which is known to utilize the carbon dioxide pathway with hydrogen acting as an electron donor for methane formation

Suspended growth kinetic analysis on biogas generation from newly isolated anaerobic bacterial communities for palm oil mill effluent at mesophilic temperature

RSC Advances ◽  
2014 ◽  
Vol 4 (110) ◽  
pp. 64659-64667 ◽  
Author(s):  
Yee-Shian Wong ◽  
Tjoon Tow Teng ◽  
Soon-An Ong ◽  
Norhashimah Morad ◽  
Mohd Rafatullah
Keyword(s):  
Kinetic Analysis ◽  
Palm Oil ◽  
Bacterial Communities ◽  
Anaerobic Degradation ◽  
Growth Kinetic ◽  
Palm Oil Mill Effluent ◽  
Suspended Growth ◽  
Palm Oil Mill ◽  
Mesophilic Temperature

The anaerobic degradation of palm oil mill effluent (POME) was carried out under mesophilic temperature in an anaerobic suspended growth closed bioreactor (ASGCB).

scholarly journals Characteristics and Microbial Succession in Co-Composting of Oil Palm Empty Fruit Bunch and Partially Treated Palm Oil Mill Effluent

2009 ◽  
Vol 3 (1) ◽  
pp. 87-95 ◽  
Author(s):  
Azhari Samsu Baharuddin ◽  
Nakamura Kazunori ◽  
Suraini Abd-Aziz ◽  
Meisam Tabatabaei ◽  
Nor’ Aini Abdul Rahman ◽  
...  
Keyword(s):  
Palm Oil ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Cellulase Activity ◽  
Pilot Scale ◽  
Palm Oil Mill Effluent ◽  
Cellulolytic Enzymes ◽  
Empty Fruit Bunch ◽  
Microbial Succession ◽  
Composting Process ◽  
Palm Oil Mill

Microbial communities and cellulolytic enzymes activities were analyzed during the co-composting of empty fruit bunch (EFB) and partially treated palm oil mill effluent (POME) in pilot scale. The physicochemical parameters were also measured during the composting. The diversity of the bacterial community was investigated using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The results indicated that the composting process of EFB with partially treated POME was dominated by uncultured bacteria species. The dominant bacterial group changed from the phylum proteobacteria in the thermophilic stage to the phylum chloroflexi in the maturing stage. Scanning electron microscope (SEM) analysis exhibited the significant degradation of EFB structure during the composting process. The maximum cellulase activity for carboxymethylcellulase (CMCase), filter paperase (FPase) and β-glucosidase were 13.6, 4.1 and 20.3 U/g of dry substrate, respectively at day 30 of composting. The results of this study significantly contributed to a better understanding of mechanisms involved in co-composting process in pilot scale.

scholarly journals Potential Utilisation of Dark-Fermented Palm Oil Mill Effluent in Continuous Production of Biomethane by Self-Granulated Mixed Culture

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Safa Senan Mahmod ◽  
Azratul Madihah Azahar ◽  
Abdullah Amru Indera Luthfi ◽  
Peer Mohamed Abdul ◽  
Mohd Shahbudin Mastar ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Palm Oil ◽  
Continuous Production ◽  
Palm Oil Mill Effluent ◽  
Net Energy ◽  
Continuous Stirred Tank Reactor ◽  
Agricultural Industry ◽  
Thermophilic Conditions ◽  
Continuous Stirred Tank ◽  
Palm Oil Mill

Abstract Two-stage anaerobic digestion of palm oil mill effluent (POME) is a promising method for converting the waste from the largest agricultural industry in Southeast Asia into a clean and sustainable energy. This study investigates the degradation of acid-rich effluent from the dark fermentation stage for the production of biomethane (BioCH4) in a 30-L continuous stirred-tank reactor (CSTR). The continuous methanogenic process was operated with varied HRTs (10 - 1 day) and OLRs (4.6–40.6 gCOD/L.d−1) under thermophilic conditions. Methanothermobacter sp. was the dominant thermophilic archaea that was responsible for the production rate of 4.3 LCH4/LPOME.d−1 and methane yield of 256.77 LCH4kgCOD at HRT of 2 d, which is the lowest HRT reported in the literature. The process was able to digest 85% and 64% of the initial POME’s COD and TSS, respectively. The formation of methane producing granules (MPG) played a pivotal role in sustaining the efficient and productive anaerobic system. We report herein that the anaerobic digestion was not only beneficial in reducing the contaminants in the liquid effluent, but generating BioCH4 gas with a positive net energy gain of 7.6 kJ/gCOD.

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