Syntrophic acetate oxidation in two-phase (acid–methane) anaerobic digesters

2011 ◽  
Vol 64 (9) ◽  
pp. 1812-1820 ◽  
Author(s):  
T. Shimada ◽  
E. Morgenroth ◽  
M. Tandukar ◽  
S. G. Pavlostathis ◽  
A. Smith ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Anaerobic Digestion ◽  
Full Scale ◽  
Laboratory Scale ◽  
Gene Clone ◽  
Rrna Gene ◽  
Acetate Oxidation ◽  
Two Phase ◽  
Anaerobic Digesters ◽  
Syntrophic Acetate Oxidation

The microbial processes involved in two-phase anaerobic digestion were investigated by operating a laboratory-scale acid-phase (AP) reactor and analyzing two full-scale, two-phase anaerobic digesters operated under mesophilic (35 °C) conditions. The digesters received a blend of primary sludge and waste activated sludge (WAS). Methane levels of 20% in the laboratory-scale reactor indicated the presence of methanogenic activity in the AP. A phylogenetic analysis of an archaeal 16S rRNA gene clone library of one of the full-scale AP digesters showed that 82% and 5% of the clones were affiliated with the orders Methanobacteriales and Methanosarcinales, respectively. These results indicate that substantial levels of aceticlastic methanogens (order Methanosarcinales) were not maintained at the low solids retention times and acidic conditions (pH 5.2–5.5) of the AP, and that methanogenesis was carried out by hydrogen-utilizing methanogens of the order Methanobacteriales. Approximately 43, 31, and 9% of the archaeal clones from the methanogenic phase (MP) digester were affiliated with the orders Methanosarcinales, Methanomicrobiales, and Methanobacteriales, respectively. A phylogenetic analysis of a bacterial 16S rRNA gene clone library suggested the presence of acetate-oxidizing bacteria (close relatives of Thermacetogenium phaeum, ‘Syntrophaceticus schinkii,’ and Clostridium ultunense). The high abundance of hydrogen consuming methanogens and the presence of known acetate-oxidizing bacteria suggest that acetate utilization by acetate oxidizing bacteria in syntrophic interaction with hydrogen-utilizing methanogens was an important pathway in the second-stage of the two-phase digestion, which was operated at high ammonium-N concentrations (1.0 and 1.4 g/L). A modified version of the IWA Anaerobic Digestion Model No. 1 (ADM1) with extensions for syntrophic acetate oxidation and weak-acid inhibition adequately described the dynamic profiles of volatile acid production/degradation and methane generation observed in the laboratory-scale AP reactor. The model was validated with historical data from the full-scale digesters.

scholarly journals Identifying the abundant and active microorganisms common to full-scale anaerobic digesters

2017 ◽  
Author(s):  
Rasmus H. Kirkegaard ◽  
Simon J. McIlroy ◽  
Jannie M. Kristensen ◽  
Marta Nierychlo ◽  
Søren M. Karst ◽  
...  
Keyword(s):  
Microbial Community ◽  
Anaerobic Digestion ◽  
Wastewater Treatment Plants ◽  
Microbial Community Composition ◽  
Amplicon Sequencing ◽  
Full Scale ◽  
Rrna Gene ◽  
Anaerobic Digesters ◽  
Abundant Otus ◽  
Source Of Information

AbstractAnaerobic digestion is widely applied to treat organic waste at wastewater treatment plants. Characterisation of the underlying microbiology represents a source of information to develop strategies for improved operation. To this end, we investigated the microbial community composition of thirty-two full-scale digesters over a six-year period using 16S rRNA gene amplicon sequencing. Sampling of the sludge fed into these systems revealed that several of the most abundant populations were likely inactive and immigrating with the influent. This observation indicates that a failure to consider immigration will interfere with correlation analysis and give an inaccurate picture of the active microbial community. Furthermore, several abundant OTUs could not be classified to genus level with commonly applied taxonomies, making inference of their function unreliable. As such, the existing MiDAS taxonomy was updated to include these abundant phylotypes. The communities of individual plants surveyed were remarkably similar – with only 300 OTUs representing 80% of the total reads across all plants, and 15% of these identified as likely inactive immigrating microbes. By identifying the abundant and active taxa in anaerobic digestion, this study paves the way for targeted characterisation of the process important organisms towards an in-depth understanding of the microbial ecology of these biotechnologically important systems.

scholarly journals Syntrophic acetate oxidation replaces acetoclastic methanogenesis during thermophilic digestion of biowaste

Microbiome ◽  
2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Stefan Dyksma ◽  
Lukas Jansen ◽  
Claudia Gallert
Keyword(s):  
Anaerobic Digestion ◽  
Amplicon Sequencing ◽  
Metabolic Reconstruction ◽  
Rrna Gene ◽  
Wastewater Management ◽  
Acetate Oxidation ◽  
Mineralization Of Organic Matter ◽  
Acetoclastic Methanogenesis ◽  
Syntrophic Acetate Oxidation ◽  
Production Knowledge

Abstract Background Anaerobic digestion (AD) is a globally important technology for effective waste and wastewater management. In AD, microorganisms interact in a complex food web for the production of biogas. Here, acetoclastic methanogens and syntrophic acetate-oxidizing bacteria (SAOB) compete for acetate, a major intermediate in the mineralization of organic matter. Although evidence is emerging that syntrophic acetate oxidation is an important pathway for methane production, knowledge about the SAOB is still very limited. Results A metabolic reconstruction of metagenome-assembled genomes (MAGs) from a thermophilic solid state biowaste digester covered the basic functions of the biogas microbial community. Firmicutes was the most abundant phylum in the metagenome (53%) harboring species that take place in various functions ranging from the hydrolysis of polymers to syntrophic acetate oxidation. The Wood-Ljungdahl pathway for syntrophic acetate oxidation and corresponding genes for energy conservation were identified in a Dethiobacteraceae MAG that is phylogenetically related to known SAOB. 16S rRNA gene amplicon sequencing and enrichment cultivation consistently identified the uncultured Dethiobacteraceae together with Syntrophaceticus, Tepidanaerobacter, and unclassified Clostridia as members of a potential acetate-oxidizing core community in nine full-scare digesters, whereas acetoclastic methanogens were barely detected. Conclusions Results presented here provide new insights into a remarkable anaerobic digestion ecosystem where acetate catabolism is mainly realized by Bacteria. Metagenomics and enrichment cultivation revealed a core community of diverse and novel uncultured acetate-oxidizing bacteria and point to a particular niche for them in dry fermentation of biowaste. Their genomic repertoire suggests metabolic plasticity besides the potential for syntrophic acetate oxidation.

scholarly journals Phylogeny of Acetate-Utilizing Microorganisms in Soils along a Nutrient Gradient in the Florida Everglades

2006 ◽  
Vol 72 (10) ◽  
pp. 6837-6840 ◽  
Author(s):  
Ashvini Chauhan ◽  
Andrew Ogram
Keyword(s):  
Stable Isotope ◽  
16S Rrna ◽  
Stable Isotope Probing ◽  
Florida Everglades ◽  
Gene Clone ◽  
Rrna Gene ◽  
Acetate Oxidation ◽  
Soil Microcosms ◽  
Nutrient Gradient ◽  
Syntrophic Acetate Oxidation

ABSTRACT The consumption of acetate in soils taken from a nutrient gradient in the northern Florida Everglades was studied by stable isotope probing. Bacterial and archaeal 16S rRNA gene clone libraries from eutrophic and oligotrophic soil microcosms strongly suggest that a significant amount of acetate is consumed by syntrophic acetate oxidation in nutrient-enriched soil.

scholarly journals Anaerobic Digestion and Removal of Sulfamethoxazole, Enrofloxacin, Ciprofloxacin and Their Antibiotic Resistance Genes in a Full-Scale Biogas Plant

Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 502
Author(s):  
Andrea Visca ◽  
Anna Barra Caracciolo ◽  
Paola Grenni ◽  
Luisa Patrolecco ◽  
Jasmin Rauseo ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Anaerobic Digestion ◽  
Resistance Genes ◽  
Emerging Contaminants ◽  
Antibiotic Resistance Genes ◽  
Animal Manure ◽  
Full Scale ◽  
Antibiotic Residues ◽  
Anaerobic Digesters ◽  
One Year

Anaerobic digestion is one of the best ways to re-use animal manure and agricultural residues, through the production of combustible biogas and digestate. However, the use of antibiotics for preventing and treating animal diseases and, consequently, their residual concentrations in manure, could introduce them into anaerobic digesters. If the digestate is applied as a soil fertilizer, antibiotic residues and/or their corresponding antibiotic resistance genes (ARGs) could reach soil ecosystems. This work investigated three common soil emerging contaminants, i.e., sulfamethoxazole (SMX), ciprofloxacin (CIP), enrofloxacin (ENR), their ARGs sul1, sul2, qnrS, qepA, aac-(6′)-Ib-cr and the mobile genetic element intI1, for one year in a full scale anaerobic plant. Six samplings were performed in line with the 45-day hydraulic retention time (HRT) of the anaerobic plant, by collecting input and output samples. The overall results show both antibiotics and ARGs decreased during the anaerobic digestion process. In particular, SMX was degraded by up to 100%, ENR up to 84% and CIP up to 92%, depending on the sampling time. In a similar way, all ARGs declined significantly (up to 80%) in the digestate samples. This work shows how anaerobic digestion can be a promising practice for lowering antibiotic residues and ARGs in soil.

Application of the IWA Anaerobic Digestion Model (ADM1) for simulating full-scale anaerobic sewage sludge digestion

2005 ◽  
Vol 52 (1-2) ◽  
pp. 487-492 ◽  
Author(s):  
Y. Shang ◽  
B.R. Johnson ◽  
R. Sieger
Keyword(s):  
Wastewater Treatment ◽  
Steady State ◽  
Anaerobic Digestion ◽  
Biogas Production ◽  
Treatment Plant ◽  
Biochemical Parameter ◽  
Full Scale ◽  
Anaerobic Digesters ◽  
Biogas Yield ◽  
Volatile Solids

A steady-state implementation of the IWA Anaerobic Digestion Model No. 1 (ADM1) has been applied to the anaerobic digesters in two wastewater treatment plants. The two plants have a wastewater treatment capacity of 76,000 and 820,000 m3/day, respectively, with approximately 12 and 205 dry metric tons sludge fed to digesters per day. The main purpose of this study is to compare the ADM1 model results with full-scale anaerobic digestion performance. For both plants, the prediction of the steady-state ADM1 implementation using the suggested physico-chemical and biochemical parameter values was able to reflect the results from the actual digester operations to a reasonable degree of accuracy on all parameters. The predicted total solids (TS) and volatile solids (VS) concentration in the digested biosolids, as well as the digester volatile solids destruction (VSD), biogas production and biogas yield are within 10% of the actual digester data. This study demonstrated that the ADM1 is a powerful tool for predicting the steady-state behaviour of anaerobic digesters treating sewage sludges. In addition, it showed that the use of a whole wastewater treatment plant simulator for fractionating the digester influent into the ADM1 input parameters was successful.

scholarly journals Methanosarcinaceae and Acetate-Oxidizing Pathways Dominate in High-Rate Thermophilic Anaerobic Digestion of Waste-Activated Sludge

2013 ◽  
Vol 79 (20) ◽  
pp. 6491-6500 ◽  
Author(s):  
Dang P. Ho ◽  
Paul D. Jensen ◽  
Damien J. Batstone
Keyword(s):  
Anaerobic Digestion ◽  
Activated Sludge ◽  
Retention Time ◽  
Elevated Temperatures ◽  
Stable Carbon Isotope ◽  
High Rate ◽  
Continuous Reactor ◽  
Waste Activated Sludge ◽  
Acetate Oxidation ◽  
Syntrophic Acetate Oxidation

ABSTRACTThis study investigated the process of high-rate, high-temperature methanogenesis to enable very-high-volume loading during anaerobic digestion of waste-activated sludge. Reducing the hydraulic retention time (HRT) from 15 to 20 days in mesophilic digestion down to 3 days was achievable at a thermophilic temperature (55°C) with stable digester performance and methanogenic activity. A volatile solids (VS) destruction efficiency of 33 to 35% was achieved on waste-activated sludge, comparable to that obtained via mesophilic processes with low organic acid levels (<200 mg/liter chemical oxygen demand [COD]). Methane yield (VS basis) was 150 to 180 liters of CH4/kg of VSadded. According to 16S rRNA pyrotag sequencing and fluorescence in situ hybridization (FISH), the methanogenic community was dominated by members of theMethanosarcinaceae, which have a high level of metabolic capability, including acetoclastic and hydrogenotrophic methanogenesis. Loss of function at an HRT of 2 days was accompanied by a loss of the methanogens, according to pyrotag sequencing. The two acetate conversion pathways, namely, acetoclastic methanogenesis and syntrophic acetate oxidation, were quantified by stable carbon isotope ratio mass spectrometry. The results showed that the majority of methane was generated by nonacetoclastic pathways, both in the reactors and in off-line batch tests, confirming that syntrophic acetate oxidation is a key pathway at elevated temperatures. The proportion of methane due to acetate cleavage increased later in the batch, and it is likely that stable oxidation in the continuous reactor was maintained by application of the consistently low retention time.

Biogas production from cheese whey wastewater: laboratory- and full-scale studies

2014 ◽  
Vol 69 (6) ◽  
pp. 1320-1325 ◽  
Author(s):  
K. Stamatelatou ◽  
N. Giantsiou ◽  
V. Diamantis ◽  
C. Alexandridis ◽  
A. Alexandridis ◽  
...  
Keyword(s):  
High Efficiency ◽  
Cheese Whey ◽  
Biogas Production ◽  
Oxygen Demand ◽  
Full Scale ◽  
Laboratory Scale ◽  
Phase System ◽  
Two Phase ◽  
Set Up ◽  
Cheese Production

A two-phase system for biogas production from cheese whey wastewater (CWW) was designed, set up and operated at laboratory and full scale for a whole cheese production season (8–9 months). The high efficiency and stability of the laboratory-scale system was demonstrated under various organic loading rates (OLRs) reaching 13 g chemical oxygen demand (COD) L−1d−1 and producing up to 9 L L−1d−1 of biogas (approximately 55% in methane). The COD removal was above 95% and the pH was maintained above 6.3 without any chemical addition. The full-scale system was operated at lower OLRs than its normal capacity, following the good response and high stability in disturbances of the laboratory-scale unit.

Extracellular enzymes and acidogen profiles of a laboratory-scale two-phase anaerobic digestion system

1992 ◽  
Vol 27 (1) ◽  
pp. 43-47 ◽  
Author(s):  
Kathleen Kaseng ◽  
Korina Ibrahim ◽  
S.V. Paneerselvam ◽  
R.S. Hassan
Keyword(s):  
Anaerobic Digestion ◽  
Extracellular Enzymes ◽  
Laboratory Scale ◽  
Two Phase
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