Characteristics of different fractions of microbial flocs and their role in membrane fouling

2011 ◽  
Vol 63 (2) ◽  
pp. 262-269 ◽  
Author(s):  
H. J. Lin ◽  
W. J. Gao ◽  
K. T. Leung ◽  
B. Q. Liao
Keyword(s):  
Microbial Community ◽  
Membrane Fouling ◽  
Extracellular Polymeric Substances ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Sludge Flocs ◽  
Microbial Flocs ◽  
Thermomechanical Pulping ◽  
Gradient Gel Electrophoresis ◽  
Sludge Cake ◽  
The Difference

Characteristics of different fractions (small flocs vs. large flocs) of sludge flocs from a submerged anaerobic membrane bioreactor treating thermomechanical pulping (TMP) whitewater were determined using various analytic techniques, including extraction and chemical analysis of extracellular polymeric substances (EPS), particle size analyzer, and polymer chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE). The results showed that the fraction of smaller flocs contained a higher level of bound EPS and had a higher fractal dimension as compared to the fraction of larger flocs. PCR-DGGE analysis indicated that there were significant differences in microbial community between the fraction of smaller flocs and large flocs. The microbial community of the smaller flocs was similar to that of the sludge cake layers, indicating the pioneering role of the microbial community in smaller flocs in membrane fouling. These findings provide a new insight in the difference of membrane fouling potential between smaller flocs and larger flocs fraction.

Impact of sludge retention time on sludge characteristics and microbial community in MBR

2011 ◽  
Vol 63 (10) ◽  
pp. 2250-2254 ◽  
Author(s):  
Yuchun Su ◽  
Jill Ruhsing Pan ◽  
Chihpin Huang ◽  
Chialing Chang
Keyword(s):  
Microbial Community ◽  
Retention Time ◽  
Membrane Fouling ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Membrane Surface ◽  
Sludge Characteristics ◽  
Sludge Retention Time ◽  
Gradient Gel Electrophoresis ◽  
Microbial Products ◽  
The Impact

In this study, the impact of sludge retention time (SRT) on sludge characteristics and microbial community and the effect on membrane fouling in membrane bioreactor (MBR) was investigated. The results show that MBR with longer SRT has less fouling propensity, in agreement with other studies, despite the fact that the MBR with longer SRT contained higher MLSS and smaller particle size. However, much more soluble microbial products (SMPs) were released in MBR with shorter SRT. More slime on the membrane surface was observed in MBR with shorter SRT while sludge cakes formed on the membrane surface in MBR with longer SRT. The results show that SMP contributes to the severe fouling observed in MBR with shorter SRT, which is in agreement with other studies showing that SMPs were the major foulants in MBR. Under different SRTs of operation, the bacterial community structures of the sludge obtained by use of polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) were almost identical, but those on the membrane surface differed substantially. It suggests that, although SRT has impact on sludge characteristics, it doesn't affect the microbial community in the suspension.

Correlation between membrane fouling and soluble/colloidal organic substances in membrane bioreactors for municipal wastewater treatment

2005 ◽  
Vol 51 (6-7) ◽  
pp. 1-8 ◽  
Author(s):  
B. Lesjean ◽  
S. Rosenberger ◽  
C. Laabs ◽  
M. Jekel ◽  
R. Gnirss ◽  
...  
Keyword(s):  
Membrane Fouling ◽  
Extracellular Polymeric Substances ◽  
Municipal Wastewater ◽  
Membrane Bioreactors ◽  
Size Exclusion ◽  
Water Phase ◽  
Parallel Operation ◽  
Municipal Wastewater Treatment ◽  
Operational Conditions ◽  
The Difference

Two similar membrane bioreactors of 2 m3 each were operated in parallel over two years under the same operational conditions, fed with the same municipal wastewater. The only process and operational difference between both pilot plants was the position of the denitrification zone (pre-denitrification in pilot 1 and post-denitrification in pilot 2). Despite parallel operation, the two MBRs exhibited different fouling rates and decreases in permeability. These differences could not be accounted for by MLSS concentrations, loading rates, or filtration flux. In a one-year investigation, soluble and colloidal organic material in the activated sludge of both MBR was regularly analysed by spectrophotometric and Size Exclusion Chromatography (SEC) methods. The larger organic molecules present in the sludge water phase (i.e. polysaccharides, proteins and organic colloids) originating from microbial activity (extracellular polymeric substances) were found to impact on the fouling and to explain the difference in membrane performance between the two MBR units. In both pilot plants, a linear relationship could be clearly demonstrated between the fouling rate of the membrane and the concentration of polysaccharides in the sludge water phase during a 5 month operational period at an SRT of 8 days.

Study of microbial community of brewery-treating granular sludge by denaturing gradient gel electrophoresis of 16S rRNA gene

2001 ◽  
Vol 43 (1) ◽  
pp. 77-82 ◽  
Author(s):  
O.-C. Chan ◽  
W.-T. Liu ◽  
H. H. Fang
Keyword(s):  
Microbial Community ◽  
16S Rrna ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Granular Sludge ◽  
Upflow Anaerobic Sludge Blanket ◽  
Anaerobic Sludge ◽  
Rrna Gene ◽  
Related Sequence ◽  
Gradient Gel Electrophoresis

The microbial community structure of granular sludge from an upflow anaerobic sludge blanket (UASB) reactor treating brewery effluent was studied by denaturing gradient gel electrophoresis (DGGE). Twelve major bands were observed in the DGGE fingerprint for the Bacteria domain and four bands for the Archaea domain. Of the bacterial bands observed, six were successfully purified and sequenced. Among them, three were related to the gram-positive low G+C group, one to the Delta subclass of the Proteobacteria, one to the Gamma subclass, and one to the Cytophaga group with no close related sequence. The 16S rRNA sequences of the four archaeal bands were closely associated with Methanosaeta concilii and Methanobacterium formicum.

Microbial Community Analysis of Underground Drinking Water Using Denaturing Gradient Gel Electrophoresis and Flow Cytometry Technologies

2014 ◽  
Vol 700 ◽  
pp. 519-524 ◽  
Author(s):  
Jie Liu ◽  
Yan Li Ding ◽  
Mark Bartlam ◽  
Ying Ying Wang
Keyword(s):  
Drinking Water ◽  
Microbial Community ◽  
Rural Areas ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Poyang Lake ◽  
Removal Rate ◽  
Underground Water ◽  
Water Sources ◽  
Gradient Gel Electrophoresis ◽  
Bacteria And Fungi

Underground water is directly used as drinking water in most rural areas of developing countries due to limitations in infrastructure. As an important indicator of drinking water quality, however, microbial quality has been largely ignored for a long time. Microbial quality poses a great threat to the safety of underground drinking water, especially in rural areas. The current study compared microbial abundance and community structure of three different water sources, i.e. underground water, Poyang Lake and Hai River, combined with flow cytometry (FCM) and denaturing gradient gel electrophoresis (DGGE). FCM results showed that the bacterial concentration of underground water is the lowest (1.037×106cell/ml) of the three water sources, but still approximates that of the Poyang Lake. The removal rate of bacteria after filtration through a 0.45μm-pore-size filter is 98.16% in underground water. The removal rate for Poyang Lake and Hai River is much lower (i.e. 66.57% and 74.17% respectively). DGGE profiles demonstrated that the microbial community structure in underground water shares higher similarity to Poyang Lake (51.0% and 53.1% similarity for bacteria and fungi respectively) than Hai River. The microbial diversity index (i.e. Shannon-Weaver index) for bacteria and fungi are 2.906 and 2.847 respectively in underground water, which is lower than in Poyang Lake. The evenness (i.e. Simpson index) of groundwater was lowest among the three water sources tested. The results suggested that groundwater has a complex microbial community and hence it is critical to apply necessary hygienic barriers to remove microbes for the safety of underground drinking water.

Spatial variation in microbial community structure, richness, and diversity in an alluvial aquifer

2012 ◽  
Vol 58 (9) ◽  
pp. 1135-1151 ◽  
Author(s):  
P.G. Medihala ◽  
J.R. Lawrence ◽  
G.D.W. Swerhone ◽  
D.R. Korber
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Spatial Variability ◽  
Microbial Communities ◽  
Microbial Community Structure ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Microbial Community Composition ◽  
Alluvial Aquifer ◽  
Gradient Gel Electrophoresis

Relatively little is known regarding the spatial variability of microbial communities in aquifers where well fouling is an issue. In this study 2 water wells were installed in an alluvial aquifer located adjacent to the North Saskatchewan River and an associated piezometer network developed to facilitate the study of microbial community structure, richness, and diversity. Carbon utilization data analysis revealed reduced microbial activity in waters collected close to the wells. Functional PCR and quantitative PCR analysis indicated spatial variability in the potential for iron-, sulphate-, and nitrate-reducing activity at all locations in the aquifer. Denaturing gradient gel electrophoresis analysis of aquifer water samples using principal components analyses indicated that the microbial community composition was spatially variable, and denaturing gradient gel electrophoresis sequence analysis revealed that bacteria belonging to the genera Acidovorax , Rhodobacter , and Sulfuricurvum were common throughout the aquifer. Shannon’s richness (H′) and Pielou’s evenness (J′) indices revealed a varied microbial diversity (H′ = 1.488–2.274) and an even distribution of microbial communities within the aquifer (J′ = 0.811–0.917). Overall, these analyses revealed that the aquifer’s microbial community varied spatially in terms of composition, richness, and metabolic activity. Such information may facilitate the diagnosis, prevention, and management of fouling.

Microbial community analysis in the autotrophic denitrification process using spent sulfidic caustic by denaturing gradient gel electrophoresis of PCR-amplified genes

2011 ◽  
Vol 63 (3) ◽  
pp. 475-483 ◽  
Author(s):  
J. -H. Lee ◽  
S. -M. Lee ◽  
G. -C. Choi ◽  
H. -S. Park ◽  
D. -H. Kang ◽  
...  
Keyword(s):  
Microbial Community ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Carbon Sources ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Autotrophic Denitrification ◽  
High Concentration ◽  
Spent Sulfidic Caustic ◽  
Gradient Gel Electrophoresis ◽  
Sulfidic Caustic

Spent sulfidic caustic (SSC) produced from petrochemical plants contains a high concentration of hydrogen sulfide and alkalinity, and some almost non-biodegradable organic compounds such as benzene, toluene, ethylbenzene and xylenes (BTEX). SSC is mainly incinerated with auxiliary fuel, leading to secondary pollution problems. The reuse of this waste is becoming increasingly important from economic and environmental viewpoints. To denitrify wastewater with low COD/N ratio, additional carbon sources are required. Thus, autotrophic denitrification has attracted increasing attention. In this study, SSC was injected as an electron donor for sulfur-based autotrophic denitrification in the modified Ludzack-Ettinger (MLE) process. The efficiencies of nitrification, COD, and total nitrogen (TN) removal were evaluated with varying SSC dosage. Adequate SSC injection exhibited stable autotrophic denitrification. No BTEX were detected in the monitored BTEX concentrations of the effluent. To analyse the microbial community of the MLE process, PCR-DGGE based on 16 S rDNA with EUB primers, TD primers and nirK gene with nirK primers was performed in order to elucidate the application of the MLE process to SSC.

Start-up of bio-hydrogen production reactor seeded with sewage sludge and its microbial community analysis

2005 ◽  
Vol 52 (1-2) ◽  
pp. 115-121 ◽  
Author(s):  
M.L. Gong ◽  
N.Q. Ren ◽  
D.F. Xing
Keyword(s):  
Microbial Community ◽  
Sewage Sludge ◽  
Hydrogen Production ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Organic Loading Rate ◽  
Start Up ◽  
Gradient Gel Electrophoresis ◽  
Continuously Stirred Tank Reactor

Start-up of a continuously stirred tank reactor for bio-hydrogen production under different initial organic loading rate (OLR) of 3, 7 and 10 kgCOD/m3 d, respectively, was carried out with sewage sludge as inoculum. Molasses wastewater was used as substrate and hydraulic retention time was kept at 6 h. This study aimed to assess OLR on the formation of fermentation types and the structure of microbial communities during the start-up period. It was found that at an initial OLR of 7 kgCOD/m3 d and an initial biomass of 6.24 gVSS/L, an equilibrial microbial community of ethanol-type fermentation could be established within 30 days. The observed average specific hydrogen production rate was 276 mLH2/gVSS d, which was 40% higher than that of the one acclimated with 3 kgCOD/m3 d. Based on denaturing gradient gel electrophoresis profiles, significant microbial population shifts took place at the first 15 days, but a longer period up to 30 days was required to establish a microbial community with stable metabolic activity.

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