Short-term effect of ammonia concentration and salinity on activity of ammonia oxidizing bacteria

2010 ◽  
Vol 61 (12) ◽  
pp. 3008-3016 ◽  
Author(s):  
J. Claros ◽  
E. Jiménez ◽  
L. Borrás ◽  
D. Aguado ◽  
A. Seco ◽  
...  
Keyword(s):  
Pilot Plant ◽  
Nitrogen Concentration ◽  
Mathematical Expression ◽  
Ammonia Concentration ◽  
Ammonia Removal ◽  
Free Ammonia ◽  
Biological Nutrient Removal ◽  
Substrate Affinity ◽  
Ammonia Oxidizing Bacteria ◽  
Maximum Value

A continuously aerated SHARON (single reactor high activity ammonia removal over nitrite) system has been operated to achieve partial nitritation. Two sets of batch experiments were carried out to study the effect of ammonia concentration and salinity on the activity of ammonia-oxidizing bacteria (AOB). Activity of AOB raised as free ammonia concentration was increased reaching its maximum value at 4.5 mg NH3-N l−1. The half saturation constant for free ammonia was determined (KNH3 = 0.32 mg NH3-N l−1). Activity decreased at TAN (total ammonium–nitrogen) concentration over 2,000 mg NH4-N l−1. No free ammonia inhibition was detected. The effect of salinity was studied by adding different concentrations of different salts to the biomass. No significant differences were observed between the experiments carried out with a salt containing or not containing NH4. These results support that AOB are inhibited by salinity, not by free ammonia. A mathematical expression to represent this inhibition is proposed. To compare substrate affinity and salinity inhibitory effect on different AOB populations, similar experiments were carried out with biomass from a biological nutrient removal pilot plant. The AOB activity reached its maximum value at 0.008 mg NH3-N l−1 and decreased at TAN concentration over 400 mg NH4-N l−1. These differences can be explained by the different AOB predominating species: Nitrosomonas europaea and N. eutropha in the SHARON biomass and Nitrosomonas oligotropha in the pilot plant.

scholarly journals ComammoxNitrospiraare the dominant ammonia oxidizers in a mainstream low dissolved oxygen nitrification reactor

2018 ◽  
Author(s):  
Paul Roots ◽  
Yubo Wang ◽  
Alex F. Rosenthal ◽  
James S. Griffin ◽  
Fabrizio Sabba ◽  
...  
Keyword(s):  
Dissolved Oxygen ◽  
Activated Sludge ◽  
Removal Rate ◽  
Ammonia Removal ◽  
Biological Nutrient Removal ◽  
Ammonia Oxidizing Bacteria ◽  
Ammonia Oxidizers ◽  
Total Abundance ◽  
Low Dissolved Oxygen ◽  
Activated Sludge Processes

AbstractRecent findings show that a subset of bacteria affiliated withNitrospira, a genus known for its importance in nitrite oxidation for biological nutrient removal applications, are capable ofcompleteammoniaoxidation (comammox) to nitrate. Early reports suggested that they were absent or present in low abundance in most activated sludge processes, and thus likely functionally irrelevant. Here we show the accumulation of comammoxNitrospirain a nitrifying sequencing batch reactor operated at low dissolved oxygen (DO) concentrations. Actual mainstream wastewater was used as influent after primary settling and an upstream pre-treatment process for carbon and phosphorus removal. The ammonia removal rate was stable and exceeded that of the treatment plant’s parallel full-scale high DO nitrifying activated sludge reactor. 16S rRNA sequencing showed a steady accumulation ofNitrospirato 53% total abundance and a decline in conventional ammonia oxidizing bacteria to <1% total abundance over 400+ days of operation. After ruling out other known ammonia oxidizers, qPCR confirmed the accumulation of comammoxNitrospirabeginning around day 200, to eventually comprise 94% of all detectedamoAand 4% of total bacteria by day 407. Quantitative fluorescence in-situ hybridization confirmed the increasing trend and high relative abundance ofNitrospira. These results demonstrate that comammox can be metabolically relevant to nitrogen transformation in wastewater treatment, and can even dominate the ammonia oxidizing community. Our results suggest that comammox may be an important functional group in energy efficient nitrification systems designed to operate at low DO levels.

scholarly journals Efficiency of the Integrated Nitrogen Removal Device to Remove Ammonia in a Hog House

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Chen Lyu ◽  
Na Li ◽  
Ming Li ◽  
Yiyan Lyu ◽  
Zhaoying Liu ◽  
...  
Keyword(s):  
Nitrogen Concentration ◽  
Environmental Parameters ◽  
Ammonia Concentration ◽  
Ammonia Nitrogen ◽  
Ion Concentration ◽  
Ammonia Oxidizing Bacteria ◽  
Ammonia Gas ◽  
Average Temperature ◽  
New Methods ◽  
Functional Strain

It is crucial to explore new methods to deal with ammonia pollution in hog barns. In this experiment, ammonia gas generated from the decomposition of nitrogenous organic matter, such as feed and manure in hog barns, was studied. Growing environmental parameters monitored included temperature, humidity, and ammonia nitrogen concentration. For 92 days between March and May, ammonia emissions were characterized by monitoring and collecting the ammonia concentration during the selected time. The results showed that the average temperature in the hog house was 18.2 ± 2.7°C, the humidity was 62.7 ± 0.3%, and the average ammonia concentration range was 17.7∼23.1 mg m−3. The collected ammonia-nitrogen-containing wastewater that entered the denitrification device showed 173, 232, 201, and 280 mgNH4-N/L, respectively. An integrated denitrification device with anaerobic ammonia-oxidizing bacteria as a functional strain was used for denitrification treatment. Through the change of ion concentration in the incoming and outgoing water, an 85.5% average denitrification efficiency was calculated according to the denitrification reaction chemical formula. Thus, the results presented here provide data support for the future use of microbial denitrification equipment to treat ammonia in hog houses.

scholarly journals Light as a Novel Inhibitor of Nitrite-Oxidizing Bacteria (NOB) for the Mainstream Partial Nitrification of Wastewater Treatment

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 346
Author(s):  
Keugtae Kim ◽  
Yong-Gyun Park
Keyword(s):  
Wastewater Treatment ◽  
Blue Light ◽  
Nitrogen Removal ◽  
Municipal Wastewater ◽  
Partial Nitrification ◽  
Biological Nutrient Removal ◽  
Nitrite Accumulation ◽  
Light Illumination ◽  
Ammonia Oxidizing Bacteria ◽  
Nitrite Oxidizing Bacteria

Conventional biological nutrient removal processes in municipal wastewater treatment plants are energy-consuming, with oxygen supply accounting for 45–75% of the energy expenditure. Many recent studies examined the implications of the anammox process in sidestream wastewater treatment to reduce energy consumption, however, the process did not successfully remove nitrogen in mainstream wastewater treatment with relatively low ammonia concentrations. In this study, blue light was applied as an inhibitor of nitrite-oxidizing bacteria (NOB) in a photo sequencing batch reactor (PSBR) containing raw wastewater. This simulated a biological nitrogen removal system for the investigation of its application potential in nitrite accumulation and nitrogen removal. It was found that blue light illumination effectively inhibited NOB rather than ammonia-oxidizing bacteria due to their different sensitivity to light, resulting in partial nitrification. It was also observed that the NOB inhibition rates were affected by other operational parameters like mixed liquor suspended solids (MLSS) concentration and sludge retention time (SRT). According to the obtained results, it was concluded that the process efficiency of partial nitrification and anammox (PN/A) could be significantly enhanced by blue light illumination with appropriate MLSS concentration and SRT conditions.

Nitrification preservation in activated sludge during curative bulking chlorination

2003 ◽  
Vol 47 (11) ◽  
pp. 85-92 ◽  
Author(s):  
E. Cotteux ◽  
P. Duchene
Keyword(s):  
Activated Sludge ◽  
Pilot Plant ◽  
Wastewater Treatment Plants ◽  
Oxygen Uptake Rate ◽  
Ammonia Concentration ◽  
Synthetic Wastewater ◽  
Biological Wastewater Treatment ◽  
Simple Method ◽  
Method Of Measurement ◽  
The Impact

The bulking that occurs in biological wastewater treatment plants using activated sludge is very often controlled by the injection of sodium hypochlorite into the return activated sludge (RAS) stream. In the present study undertaken at two pilot plants fed with synthetic wastewater, the impact of the pass frequency of the sludge at the chlorine dosing point on the nitrifying flora is analysed. The pass frequency is one for the pilot plant 1 and two for the pilot plant 2. A dose of chlorine of 4.85 ± 0.05 g/kg/MLVSS per day was applied at both pilots. The preservative effect on nitrifying activity of the lowest concentration of chlorine at the dosing point and therefore of the highest pass frequency was evidenced. Among other tools, a simple method of measurement of the oxygen uptake rate enabled us to monitor the effect of chlorination on nitrification before recording an increase in the ammonia concentration in the bulking.

Application of a synthetic zeolite as a storage medium in SBRs to achieve the stable partial nitrification of ammonium

2019 ◽  
Vol 5 (2) ◽  
pp. 287-295 ◽  
Author(s):  
Jing Chen ◽  
Xiaojun Wang ◽  
Zhenguo Chen ◽  
Xinghui Feng ◽  
Xiaokun Chen
Keyword(s):  
Synthetic Zeolite ◽  
Free Ammonia ◽  
Partial Nitrification ◽  
Ammonia Oxidizing Bacteria ◽  
Growth Substrate ◽  
Storage Medium ◽  
High Concentration

Free ammonia (FA) is the growth substrate for ammonia-oxidizing bacteria (AOB), but a high concentration of FA could also inhibit AOB activities.

Improvement of ammonia removal in activated sludge process with feedforward-feedback aeration controllers

2006 ◽  
Vol 53 (4-5) ◽  
pp. 125-132 ◽  
Author(s):  
Darko Vrečko ◽  
Nadja Hvala ◽  
Aljaž Stare ◽  
Olga Burica ◽  
Marjeta Stražar ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Pilot Plant ◽  
Pi Controller ◽  
Ammonia Removal ◽  
Activated Sludge Process ◽  
Aerobic Reactor

In the paper three linear aeration controllers that can be easily implemented are presented and evaluated on the activated sludge process pilot plant. Controllers differ according to the information that is used about the process, which can be oxygen in the last aerobic reactor, ammonia in the last aerobic reactor and ammonia in the influent. The aeration controllers that are addressed are: oxygen cascade PI controller, ammonia cascade PI controller and ammonia feedforward-cascade PI controller. Experiments show that, in comparison with the oxygen cascade PI controller, the ammonia cascade PI controller allows better control of effluent ammonia and airflow savings of around 23%, while the ammonia feedforward-cascade PI controller gives the best reduction of ammonia peaks and can save up to 45% of the airflow.

Nutrient removal from wastewaters using high performance materials

2003 ◽  
Vol 47 (11) ◽  
pp. 101-107 ◽  
Author(s):  
I.D.R. Mackinnon ◽  
K. Barr ◽  
E. Miller ◽  
S. Hunter ◽  
T. Pinel
Keyword(s):  
Wastewater Treatment ◽  
Total Nitrogen ◽  
Nutrient Removal ◽  
Pilot Plant ◽  
High Performance ◽  
Wastewater Treatment Plants ◽  
Significant Proportion ◽  
Biological Nutrient Removal ◽  
Wastewater Stream ◽  
Rich Solution

Return side streams from anaerobic digesters and dewatering facilities at wastewater treatment plants (WWTPs) contribute a significant proportion of the total nitrogen load on a mainstream process. Similarly, significant phosphate loads are also recirculated in biological nutrient removal (BNR) wastewater treatment plants. Ion exchange using a new material, known by the name MesoLite, shows strong potential for the removal of ammonia from these side streams and an opportunity to concurrently reduce phosphate levels. A pilot plant was designed and operated for several months on an ammonia rich centrate from a dewatering centrifuge at the Oxley Creek WWTP, Brisbane, Australia. The system operated with a detention time in the order of one hour and was operated for between 12 and 24 hours prior to regeneration with a sodium rich solution. The same pilot plant was used to demonstrate removal of phosphate from an abattoir wastewater stream at similar flow rates. Using MesoLite materials, &gt;90% reduction of ammonia was achieved in the centrate side stream. A full-scale process would reduce the total nitrogen load at the Oxley Creek WWTP by at least 18%. This reduction in nitrogen load consequently improves the TKN/COD ratio of the influent and enhances the nitrogen removal performance of the biological nutrient removal process.

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