Organic carbon effect on nitrifying bacteria in a mixed culture

2010 ◽  
Vol 61 (11) ◽  
pp. 2951-2956 ◽  
Author(s):  
L. Racz ◽  
T. Datta ◽  
R. K. Goel

This study investigated the effect of organic carbon source on ammonia oxidizing community in single sludge laboratory scale sequencing batch reactors (SBR). Two sequencing batch reactors performing simultaneous carbon oxidation and nitrification were operated. Operationally and functionally, these two reactors were identical, except that one reactor was fed peptone and sodium acetate, and the other was fed glucose and sodium acetate as external organic carbon sources. The peptone-fed reactor had 98.1±1.84% COD removal and 97.3±6.69% NH3-N oxidation. The glucose-fed reactor had 99.1±1.29% COD removal and 99.4±0.76% NH3-N oxidation. The reactor fed with peptone, a complex organic carbon source comprised of enzymatic digests of animal proteins, had greater diversity in both the heterotrophic bacterial community and the ammonia oxidizing bacteria community than in the reactor fed with glucose, a simple sugar as evidenced by automated ribosomal intergenic spacer analysis (ARISA) and terminal restriction fragment length polymorphism (TRFLP) experiments respectively.

2017 ◽  
Vol 75 (9) ◽  
pp. 2149-2156 ◽  
Author(s):  
A. Ito ◽  
H. Kawakami ◽  
N. Ishikawa ◽  
M. Ito ◽  
T. Oikawa ◽  
...  

Accelerated release of potassium (K), magnesium (Mg) and phosphorus (P) from surplus activated sludge (SAS) was investigated to develop a new system for the recovery of the elements. Anaerobic cultivation of SAS during 24 h released 78% of K and about 50% of Mg and P from SAS more effectively compared to aerobic cultivation (K: 40%, Mg: 15%, P: 15%). Furthermore, the addition of sodium acetate as an organic carbon source remarkably accelerated the release of K, Mg and P from SAS under anaerobic condition. However, no increase in the maximum release efficiencies was observed. The elements released from SAS could be transferred to separate liquid with the existing mechanical thickener and be recovered as MgKPO4 by some additional process. Furthermore, the removal of the elements from SAS would inhibit the formation of struvite causing the blockage of sludge transport pipe after anaerobic digestion process of thickened sludge.


2013 ◽  
Vol 726-731 ◽  
pp. 2589-2593
Author(s):  
Tao Yang ◽  
Pei Ying Wu ◽  
Zhan Sheng Zhao ◽  
Hua Wei Xu ◽  
Gao Zhi Lv

Enriched domestication in SBR was used to improve the proportion of aerobic denitrifiers in activated sludge, and actual power plant wastewater was treated, which proved the existence of aerobic denitrification. But at the later stage, because of carbon deficiency, aerobic denitrifiers were inhibited and NO3--N accumulated. Sodium acetate used as external organic carbon source was added when reaction carried on 3.5 hours to improve the COD/NH4+-N ratio from 6.5 to 10, effluent NO3--N concentration was 3.6 mg\L, average removal efficiency of TN was 90%, which could improve the aerobic denitrification performance of whole system effectively.


2006 ◽  
Vol 6 (3) ◽  
pp. 105-113 ◽  
Author(s):  
D.Y. Zhang ◽  
G.H. Li ◽  
Y. Wang ◽  
G.Z. Zhou

Many nitrate pollution cases exceed the threshold as recommended by the World Health Organization (50 mg NO3/L) and by the USA (10 mg N/L) for drinking water. In-situ denitrification was regarded as a good method to decrease nitrate contamination but is restricted by carbon absent in groundwater. Considering the disadvantages of known carbon sources, this paper provided slow-release organic carbon-source (SOC) technique to solve the problem and the results showed that SOC materials showed good performance during simulated groundwater denitrification. Structure analysis suggested that hydroxy chemical bond existed between PVA and starch in SOC and surface configuration changed with materials dissolving into water. After seven days of domestication, with 40–50 mg/L initial NO3-N, denitrification efficiency increased from 80.6% to 90.7% and the real COD consumption per N-NO3 reduction was 1.82–3.73 with 2.79 as average. Denitrification process followed the law of zero order kinetics and the parameter of denitrification dynamics, K, was from 0.1366 to 0.1873. It was suggested that SOC was a potential carbon source material (electron donor) suitable for in-situ groundwater denitrification.


Aquaculture ◽  
2021 ◽  
pp. 736669
Author(s):  
Rildo José Vasconcelos de Andrade Brazil ◽  
Elizabeth Pereira dos Santos ◽  
Gisely Karla de Almeida Costa ◽  
Clarissa Vilela Figueiredo Campos ◽  
Suzianny Maria Bezerra Cabral da Silva ◽  
...  

1999 ◽  
Vol 39 (6) ◽  
pp. 61-68 ◽  
Author(s):  
Klangduen Pochana ◽  
Jürg Keller

Experiments have been performed to gain an understanding of the conditions and processes governing the occurrence of SND in activated sludge systems. Sequencing batch reactors (SBRs) have been operated under controlled conditions using the wastewater from the first anaerobic pond in an abattoir wastewater treatment plant. Under specific circumstances, up to 95% of total nitrogen removal through SND has been found in the system. Carbon source and oxygen concentrations were found to be important process parameters. The addition of acetate as an external carbon source resulted in a significant increase of SND activity in the system. Stepwise change of DO concentration has also been observed in this study. Experiments to determine the effect of the floc size on SND have been performed in order to test the hypothesis that SND is a physical phenomenon, governed by the diffusion of oxygen into the activated sludge flocs. Initial results support this hypothesis but further experimental confirmation is still required.


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