Acrylic acid removal from synthetic wastewater and industrial wastewater using Ralstonia solanacearum and Acidovorax avenae isolated from a wastewater treatment system manufactured with polyacrylonitrile fiber

2009 ◽  
Vol 60 (11) ◽  
pp. 3011-3016 ◽  
Author(s):  
C. C. Wang ◽  
C. M. Lee ◽  
A. S. Wu

Ralstonia solanacearum and Acidovorax avenae were isolated from a wastewater treatment system manufactured with polyacrylonitrile fiber. The investigation goal is to elucidate the effectiveness of Ralstonia solanacearum and Acidovorax avenae in treating acrylic acid from synthetic wastewater and industrial wastewater. The results reveal that Ralstonia solanacearum and Acidovorax avenae could utilize acrylic acid from synthetic wastewater for growth, when the initial acrylic acid concentration was below 1,009.1 mg/l and 1,383.4 mg/l, respectively. When the acrylic acid concentration was below 606.8 mg/l, the acrylic acid removal ability reached 96.7% and 100%, respectively. Both strains could tolerate acrylamide toxicity, but only Ralstonia solanacearum could tolerate acrylonitrile toxicity. Ralstonia solanacearum and Acidovorax avenae could utilize acrylic acid from industrial wastewater for growth, when the initial acrylic acid concentration was below 1,741.1 mg/l and 1,431.2 mg/l, respectively. When the acrylic acid concentration was below 690.8 mg/l, the acrylic acid removal efficiency reached 83.5% and 62.2%, respectively. Whether the acrylic acid existed in synthetic wastewater or in industrial wastewater, the removal efficiency of acrylic acid by Ralstonia solanacearum exceeded that by Acidovorax avena.

2006 ◽  
Vol 53 (6) ◽  
pp. 181-186 ◽  
Author(s):  
C.C. Wang ◽  
C.M. Lee

The aim of this study is to isolate the acrylic acid utilizing bacteria from the ABS resin manufactured wastewater treatment system. The bacteria should have the ability to remove acrylic acid and tolerate the acrylonitrile and acrylamide toxicity. The aim is also to understand the performance of isolated pure strain for treating different initial acrylic acid concentrations from synthetic wastewater. The results are: twenty strains were isolated from the ABS resin manufactured wastewater treatment system and twelve of them could utilize 600 mg/l acrylic acid for growth. Seven of twelve strains could tolerate the acrylonitrile and acrylamide toxicity, when the concentration was below 300 mg/l. Bacillus thuringiensis was one of the seven strains and the optimum growth temperature was 32 °C. Bacillus thuringiensis could utilize acrylic acid for growth, when the initial acrylic acid concentration was below 1,690.4 mg/l. Besides this, when the initial acrylic acid concentration was below 606.8 mg/l, the acrylic acid removal efficiency exceeded 96.3%. Bacillus thuringiensis could tolerate 295.7 mg/l acrylamide and 198.4 mg/l acrylonitrile toxicity but could not tolerate 297.3 mg/l ɛ-caprolactam.


2004 ◽  
Vol 49 (5-6) ◽  
pp. 341-354 ◽  
Author(s):  
C.M. Lee ◽  
C.C. Wang

The aim of this study is to isolate denitrifying bacteria utilizing ɛ-caprolactam as the substrate, from a polyacrylonitrile fibre manufactured wastewater treatment system. The aim is also to compare the performance of PAN (polyacrylonitrile) mixed bacteria cultures acclimated to ɛ-caprolactam and isolated pure strain for treating different initial e-caprolactam concentrations from synthetic wastewater under anoxic conditions. The result showed that the PAN mixed bacteria cultures acclimated to e-caprolactam could utilize 1538.5 mg/l of ɛ-caprolactam as a substrate for denitrification. Sufficient time and about 2200 mg/l of nitrate were necessary for the complete ɛ-caprolactam removal. Paracoccus thiophilus was isolated from the polyacrylonitrile fibre manufactured wastewater treatment system and it could utilize 1722.5 mg/l of ɛ-caprolactam as a substrate for denitrification. About 3500 mg/l of nitrate was necessary for the complete removal of ɛ-caprolactam. When the initial ɛ-caprolactam concentration was below 784.3 mg/l, the removal efficiency of ɛ-caprolactam by Paracoccus thiophilus was better than that for the PAN mixed bacteria cultures. The growth of Paracoccus thiophilus was better. However, when the initial ɛ-caprolactam concentration was as high as 1445.8 mg/l, both the ɛ-caprolactam removal efficiency by Paracoccus thiophilus and Paracoccus thiophilus specific growth rate were similar to the PAN mixed bacteria cultures.


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