Kinetic and metabolic aspects of Defluviicoccus vanus-related organisms as competitors in EBPR systems

2008 ◽  
Vol 58 (8) ◽  
pp. 1693-1697 ◽  
Author(s):  
A. B. Lanham ◽  
M. A. M Reis ◽  
P. C. Lemos
Keyword(s):  
In Situ Hybridization ◽  
Volatile Fatty Acid ◽  
Consumption Rate ◽  
Carbon Sources ◽  
Aerobic Conditions ◽  
Initial Batch ◽  
Batch Tests ◽  
Consumption Rates ◽  
Pha Production

A reactor was successfully enriched (90% as shown by Fluorescence in situ Hybridization) in Defluviicoccus vanus-related organisms presenting a Glycogen Accumulating Organisms (GAO) phenotype. Initial batch tests were performed using anaerobic/aerobic conditions to assess the capacity of different carbon sources utilization frequently abundant in wastewater: acetate, propionate, butyrate, valerate and glucose. Acetate and propionate were totally consumed in the anaerobic phase as well as butyrate and valerate, though these last ones with a very low consumption rate. All substrates were converted to polyhydroxyalkanoates (PHA). Glucose had a very slight anaerobic consumption but failed to disclose a typical GAO phenotype. In aerobic conditions, again all carbon sources were readily consumed except for glucose, with acetate and propionate having the higher consumption rates. Therefore, glucose seems not be used by this type of organisms. Acetate and propionate consumption rates indicated that these GAOs could reveal good competition advantages in EBPR systems where these carbon sources are available, especially propionate. Volatile Fatty Acid (VFA) uptake in aerobic phase and consequential PHA production indicate these organisms as possible candidates for PHA production.

scholarly journals Use of Combined Microautoradiography and Fluorescence In Situ Hybridization To Determine Carbon Metabolism in Mixed Natural Communities of Uncultured Bacteria from the GenusAchromatium

2000 ◽  
Vol 66 (10) ◽  
pp. 4518-4522 ◽  
Author(s):  
N. D. Gray ◽  
R. Howarth ◽  
R. W. Pickup ◽  
J. Gwyn Jones ◽  
I. M. Head
Keyword(s):  
In Situ Hybridization ◽  
Organic Carbon ◽  
Fluorescence In Situ Hybridization ◽  
Carbon Metabolism ◽  
Carbon Sources ◽  
Uncultured Bacteria ◽  
Sulfur Bacteria ◽  
Organic Carbon Sources ◽  
Natural Communities

ABSTRACT Combined microautoradiography and fluorescence in situ hybridization (FISH) was used to investigate carbon metabolism in uncultured bacteria from the genus Achromatium. All of theAchromatium species identified in a freshwater sediment from Rydal Water, Cumbria, United Kingdom, which were distinguishable only by FISH, assimilated both [14C]bicarbonate and [14C]acetate. This extends previous findings thatAchromatium spp. present at another location could only utilize organic carbon sources. Achromatium spp., therefore, probably exhibit a range of physiologies, i.e., facultative chemolithoautotrophy, mixotrophy, and chemoorganoheterotrophy, similar to other large sulfur bacteria (e.g., Beggiatoa spp.).

scholarly journals Towards PHA production from wastes: The bioconversion potential of different activated sludge and food industry wastes into VFAs through acidogenic fermentation

2021 ◽  
Author(s):  
Gabriela Montiel-Jarillo ◽  
Teresa Gea ◽  
Adriana Artola ◽  
Javier Fuentes ◽  
Julián Carrera ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Low Cost ◽  
Carbon Sources ◽  
Olive Mill Wastewater ◽  
High Rate ◽  
Methanogenic Activity ◽  
Batch Tests ◽  
Acidogenic Fermentation ◽  
Pha Production ◽  
Olive Mill

Abstract Acidogenic fermentation of wastes produces volatile fatty acid (VFA)-rich streams that can be used as low-cost carbon sources for polyhydroxyalkanoate (PHA) production. In this study, an inoculum collected from an anaerobic reactor of a municipal WWTP was conditioned to suppress methanogenic activity. The heat-shock conditioning method of the inoculum proved to be more efficient than acid and alkaline conditioning methods for methanogen inhibition. Then, the pre-conditioned inoculum was used to determine the acidogenic potential of different wastes: three waste activated sludge (WAS) samples generated at different sludge retention times (SRTs, 2, 7 and 14 days), olive mill wastewater (OMW), glycerol, apple pomace (AP) and winterization oil cake (WOC). Batch tests were performed in quintuplicate at 37°C and pH 7. A higher degree of acidification was observed for high-rate activated sludge (2 days of SRT) (69%), followed by olive mill wastewater (OMW) (43%), while the lowest was for glycerol (16%). The results for the winterization oil cake (WOC) samples interestingly elucidated a high content of propionic acid with a high odd-to-even ratio (0.86) after fermentation. Feeding the VFA profile obtained from WOC into a PHA production system led to a significant production of 0.64 g PHA g− 1 C with 30% polyhydrobutyrate (PHB) to 69% polyhydroxyvalerate (PHV) as monomeric units of HB-co-HV, decoupling the need for a related carbon source for co-polymer production.

DNA sequence mapping in interphase and metaphase chromosomes by fluorescence in situ hybridization

1992 ◽  
Vol 50 (1) ◽  
pp. 496-497
Author(s):  
Barbara Trask ◽  
Susan Allen ◽  
Anne Bergmann ◽  
Mari Christensen ◽  
Anne Fertitta ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Dna Sequence ◽  
Dna Sequences ◽  
Dual Band ◽  
Nick Translation ◽  
Metaphase Chromosomes ◽  
Band Pass ◽  
Texas Red ◽  
Fluorescent Spot

Using fluorescence in situ hybridization (FISH), the positions of DNA sequences can be discretely marked with a fluorescent spot. The efficiency of marking DNA sequences of the size cloned in cosmids is 90-95%, and the fluorescent spots produced after FISH are ≈0.3 μm in diameter. Sites of two sequences can be distinguished using two-color FISH. Different reporter molecules, such as biotin or digoxigenin, are incorporated into DNA sequence probes by nick translation. These reporter molecules are labeled after hybridization with different fluorochromes, e.g., FITC and Texas Red. The development of dual band pass filters (Chromatechnology) allows these fluorochromes to be photographed simultaneously without registration shift.

Ultrastructural analysis of the spatial distribution of MRNA

1992 ◽  
Vol 50 (1) ◽  
pp. 552-553
Author(s):  
Gary Bassell ◽  
Robert H. Singer
Keyword(s):  
Electron Microscopy ◽  
Spatial Distribution ◽  
In Situ Hybridization ◽  
High Resolution ◽  
Nucleic Acids ◽  
Colloidal Gold ◽  
Dna Probe ◽  
Nucleotide Analogs ◽  
Gold Particles

We have been investigating the spatial distribution of nucleic acids intracellularly using in situ hybridization. The use of non-isotopic nucleotide analogs incorporated into the DNA probe allows the detection of the probe at its site of hybridization within the cell. This approach therefore is compatible with the high resolution available by electron microscopy. Biotinated or digoxigenated probe can be detected by antibodies conjugated to colloidal gold. Because mRNA serves as a template for the probe fragments, the colloidal gold particles are detected as arrays which allow it to be unequivocally distinguished from background.

1796: Analysis of Chromosomal Aneuploidy in Patients with Oligoasthenoteratozoospermia(OAT) Using Fluorescent in-situ Hybridization

2007 ◽  
Vol 177 (4S) ◽  
pp. 596-597
Author(s):  
Joseph P. Alukal ◽  
Bobby B. Najari ◽  
Wilson Chuang ◽  
Lata Murthy ◽  
Monica Lopez-Perdomo ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Chromosomal Aneuploidy

890: Multiple Fluorescence in Situ Hybridization (FISH) Probes Increase Sensitivity for Detection of Bladder Cancer

2006 ◽  
Vol 175 (4S) ◽  
pp. 287-288 ◽  
Author(s):  
Juliann M. Dziubinski ◽  
Michael F. Sarosdy ◽  
Paul R. Kahn ◽  
Mark D. Ziffer ◽  
William R. Love ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization

588: Detection of Chromosome 8 Alterations in Paired Needle Biopsy and Prostatectomy Specimens Using Fluorescence In-Situ Hybridization

2004 ◽  
Vol 171 (4S) ◽  
pp. 156-156
Author(s):  
Chandler D. Dora ◽  
Yasushi Kondo ◽  
Fusheng X. Lan ◽  
Jeffrey M. Slezak ◽  
Erik J. Bergstralh ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Needle Biopsy ◽  
Chromosome 8
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