Experimental and model-based evaluation of the role of denitrifying polyphosphate accumulating organisms at two large scale WWTPs in northern Poland

2006 ◽  
Vol 54 (8) ◽  
pp. 73-81 ◽  
Author(s):  
J. Makinia ◽  
K.-H. Rosenwinkel ◽  
M. Swinarski ◽  
E. Dobiegala
Keyword(s):  
Large Scale ◽  
Field Measurements ◽  
Model Parameters ◽  
Biological Phosphorus Removal ◽  
Batch Tests ◽  
Northern Poland ◽  
Real Wastewater ◽  
Polyphosphate Accumulating Organisms ◽  
Biological Phosphorus

The capabilities of denitrifying Polyphosphate Accumulating Organisms (DPAOs) in two large-scale plants in northern Poland performing enhanced biological phosphorus removal (EBPR) were evaluated in this study. A series of batch tests with the process biomass aimed at the measurements of phosphate release (with artificial substrate and real wastewater) and subsequent phosphate uptake under anoxic/aerobic conditions. The process kinetics were predicted using ASM2d implemented in the GPS-X ver. 4.0.2 simulation package. The results from one experimental series (summer) were used for the model calibration, whereas the results from another series (spring) were used for the model validation. The model parameters were also accurately confirmed by predictions of the accompanying field measurements in the full-scale bioreactors. The experimental and simulation results revealed that a relatively small fraction of PAO could denitrify (ηNO3,PAO=0.32). The denitrification rates associated with the anoxic storage of PP and the anoxic growth of PAO only constituted 16.0–21.0% of the denitrification rates associated with the anoxic activity of “ordinary” heterotrophs.

scholarly journals Recovery of Phosphorus in Wastewater in the Form of Polyphosphates: A Review

Processes ◽  
2022 ◽  
Vol 10 (1) ◽  
pp. 144
Author(s):  
Weiran Chu ◽  
Yi Shi ◽  
Liang Zhang
Keyword(s):  
Phosphorus Removal ◽  
Renewable Resource ◽  
Phosphate Removal ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Treatment Processes ◽  
Real Wastewater ◽  
Polyphosphate Accumulating Organisms ◽  
Chain Lengths ◽  
Biological Phosphorus

As non-renewable resource, the recovery and utilization of phosphorus from wastewater is an enduring topic. Stimulated by the advances in research on polyphosphates (polyP) as well as the development of Enhanced Biological Phosphorus Removal (EBPR) technology to achieve the efficient accumulation of polyP via polyphosphate accumulating organisms (PAOs), a novel phosphorus removal strategy is considered with promising potential for application in real wastewater treatment processes. This review mainly focuses on the mechanism of phosphorus aggregation in the form of polyP during the phosphate removal process. Further discussion about the reuse of polyP with different chain lengths is provided herein so as to suggest possible application pathways for this biosynthetic product.

A flux-based stoichiometric model of enhanced biological phosphorus removal metabolism

1998 ◽  
Vol 37 (4-5) ◽  
pp. 609-613
Author(s):  
J. Pramanik ◽  
P. L. Trelstad ◽  
J. D. Keasling
Keyword(s):  
Phosphorus Removal ◽  
Reaction Network ◽  
Bacterial Biomass ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Stoichiometric Model ◽  
Aerobic Reactor ◽  
Complete Set ◽  
Biological Phosphorus

Enhanced biological phosphorus removal (EBPR) in wastewater treatment involves metabolic cycling through the biopolymers polyphosphate (polyP), polyhydroxybutyrate (PHB), and glycogen. This cycling is induced through treatment systems that alternate between carbon-rich anaerobic and carbon-poor aerobic reactor basins. While the appearance and disappearance of these biopolymers has been documented, the intracellular pressures that regulate their synthesis and degradation are not well understood. Current models of the EBPR process have examined a limited number of metabolic pathways that are frequently lumped into an even smaller number of “reactions.” This work, on the other hand, uses a stoichiometric model that contains a complete set of the pathways involved in bacterial biomass synthesis and energy production to examine EBPR metabolism. Using the stoichiometric model we were able to analyze the role of EBPR metabolism within the larger context of total cellular metabolism, as well as predict the flux distribution of carbon and energy fluxes throughout the total reaction network. The model was able to predict the consumption of PHB, the degradation of polyP, the uptake of acetate and the release of Pi. It demonstrated the relationship between acetate uptake and Pi release, and the effect of pH on this relationship. The model also allowed analysis of growth metabolism with respect to EBPR.

Enhanced biological phosphorus removal process implemented in membrane bioreactors to improve phosphorous recovery and recycling

2003 ◽  
Vol 48 (1) ◽  
pp. 87-94 ◽  
Author(s):  
B. Lesjean ◽  
R. Gnirss ◽  
C. Adam ◽  
M. Kraume ◽  
F. Luck
Keyword(s):  
Phosphorus Removal ◽  
Theoretical Calculations ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
P Limitation ◽  
Batch Tests ◽  
P Content ◽  
On Line ◽  
Biological Phosphorus ◽  
P Removal

The enhanced biological phosphorus removal (EBPR) process was adapted to membrane bioreactor (MBR) technology. One bench-scale plant (BSP, 200-250 L) and two pilot plants (PPs, 1,000-3,000 L each) were operated under several configurations, including pre-denitrification and post-denitrification without addition of carbon source, and two solid retention times (SRT) of 15 and 26 d. The trials showed that efficient Bio-P removal can be achieved with MBR systems, in both pre- and post-denitrification configurations. EBPR dynamics could be clearly demonstrated through batch-tests, on-line measurements, profile analyses, P-spiking trials, and mass balances. High P-removal performances were achieved even with high SRT of 26 d, as around 9 mgP/L could be reliably removed. After stabilisation, the sludge exhibited phosphorus contents of around 2.4%TS. When spiked with phosphorus (no P-limitation), P-content could increase up to 6%TS. The sludge is therefore well suited to agricultural reuse with important fertilising values. Theoretical calculations showed that increased sludge age should result in a greater P-content. This could not be clearly demonstrated by the trials. This effect should be all the more significant as the influent is low in suspended solids.

scholarly journals The importance of vegetation to understand terrestrial water storage variations

2021 ◽  
Author(s):  
Tina Trautmann ◽  
Sujan Koirala ◽  
Nuno Carvalhais ◽  
Andreas Güntner ◽  
Martin Jung
Keyword(s):  
Soil Moisture ◽  
Large Scale ◽  
Water Storage ◽  
Model Parameters ◽  
Intermediate Water ◽  
Model Structure ◽  
Hydrological Models ◽  
Terrestrial Water Storage ◽  
Terrestrial Water

Abstract. So far, various studies aimed at decomposing the integrated terrestrial water storage variations observed by satellite gravimetry (GRACE, GRACE-FO) with the help of large-scale hydrological models. While the results of the storage decomposition depend on model structure, little attention has been given to the impact of the way how vegetation is represented in these models. Although vegetation structure and activity represent the crucial link between water, carbon and energy cycles, their representation in large-scale hydrological models remains a major source of uncertainty. At the same time, the increasing availability and quality of Earth observation-based vegetation data provide valuable information with good prospects for improving model simulations and gaining better insights into the role of vegetation within the global water cycle. In this study, we use observation-based vegetation information such as vegetation indices and rooting depths for spatializing the parameters of a simple global hydrological model to define infiltration, root water uptake and transpiration processes. The parameters are further constrained by considering observations of terrestrial water storage anomalies (TWS), soil moisture, evapotranspiration (ET) and gridded runoff (Q) estimates in a multi-criteria calibration approach. We assess the implications of including vegetation on the simulation results, with a particular focus on the partitioning between water storage components. To isolate the effect of vegetation, we compare a model experiment with vegetation parameters varying in space and time to a baseline experiment in which all parameters are calibrated as static, globally uniform values. Both experiments show good overall performance, but including vegetation data led to even better performance and more physically plausible parameter values. Largest improvements regarding TWS and ET were seen in supply-limited (semi-arid) regions and in the tropics, whereas Q simulations improve mainly in northern latitudes. While the total fluxes and storages are similar, accounting for vegetation substantially changes the contributions of snow and different soil water storage components to the TWS variations, with the dominance of an intermediate water pool that interacts with the fast plant accessible soil moisture and the delayed water storage. The findings indicate the important role of deeper moisture storages as well as groundwater-soil moisture-vegetation interactions as a key to understanding TWS variations. We highlight the need for further observations to identify the adequate model structure rather than only model parameters for a reasonable representation and interpretation of vegetation-water interactions.

Distributed microbial state effects on competitionin enhanced biological phosphorus removal systems

2006 ◽  
Vol 54 (1) ◽  
pp. 199-207 ◽  
Author(s):  
A.J. Schuler
Keyword(s):  
Phosphorus Removal ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Storage Product ◽  
Distributed Approach ◽  
Average System ◽  
Storage Products ◽  
Profile Characteristics ◽  
Polyphosphate Accumulating Organisms ◽  
Biological Phosphorus

Computer simulation of activated sludge population dynamics is a useful tool in process design, operation, and troubleshooting, but currently available programs rely on the assumption of “lumped,” or average, system characteristics in each reactor, such as microbial storage product contents. In reality, the states of individual bacteria are likely to vary due to variable residence times in reactors with completely mixed hydraulics. Earlier work by the present author introduced the MATLAB-based distributed state simulation program, Dissimulator 1.0, and demonstrated that distributed states may be particularly important in enhanced biological phosphorus removal (EBPR) systems, which rely on the cycling of bacteria through anaerobic and aerobic reactors to select for a population accumulating multiple microbial storage products. This paper explores the relationships between distributed state profiles, variable anaerobic and aerobic SRTs, and the process rates predicted by lumped and distributed approaches. Consistent with previous results, the lumped approach consistently predicted better EBPR performance than did the distributed approach. The primary reason for this was the presence of large fractions of polyphosphate accumulating organisms (PAOs) with depleted microbial storage product contents, which led to overestimation of process rates by the lumped approach. Distributed and lumped predictions were therefore most similar when microbial storage product depletion was minimal. The effects of variable anaerobic and aerobic SRTs on distributed profile characteristics and process rates are presented. This work demonstrated that lumped assumptions may overestimate EBPR performance, and the degree of this error is a function of the distributed state profile characteristics such as the degree to which fractions of the biomass contain depleted microbial storage product contents.

Role of Phosphate-Accumulating Bacteria in Biological Phosphorus Removal from Wastewater

2020 ◽  
Vol 56 (1) ◽  
pp. 1-14 ◽  
Author(s):  
A. G. Dorofeev ◽  
Yu. A. Nikolaev ◽  
A. V. Mardanov ◽  
N. V. Pimenov
Keyword(s):  
Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Biological Phosphorus

Biological Phophorus Removal: Study of the Main Parameters

1984 ◽  
Vol 16 (10-11) ◽  
pp. 173-185 ◽  
Author(s):  
D Malnou ◽  
M Meganck ◽  
G M Faup ◽  
M du Rostu
Keyword(s):  
Fatty Acids ◽  
Bacterial Strain ◽  
Phosphorus Removal ◽  
Volatile Fatty Acids ◽  
Organic Substances ◽  
Biological Phosphorus Removal ◽  
Batch Tests ◽  
Microbiological Examination ◽  
Biological Phosphorus ◽  
Phosphorus Storage

The biological phosphorus removal phenomenon has been studied in a modified “Phoredox” type pilot plant. The interpretation of the results obtained was facilitated by batch tests on the sludge. The influence of the duration of anaerobiosis, the presence of nitrates and various organic substances in the anaerobic zone were thus studied successively. The results obtained tend to confirm the hypothesis that biological phosphorus removal is due primarily to the bacterial strain Acinetobacter. Microbiological examination of the sludge has revealed the presence of these bacteria and that of acidogenic bacteria producing volatile fatty acids promoting the growth of Acinetobacter. Pure culture tests have confirmed the possibility of a greater phosphorus storage capability of Acinetobacter.

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