Culture of Helicobacter pylori from domestic water samples – the impact of strain variation on growth on solid and in liquid media

2006 ◽  
Vol 54 (3) ◽  
pp. 147-152 ◽  
Author(s):  
R.J. Owen ◽  
S.A. Chisholm ◽  
G. Brick ◽  
J.V. Lee ◽  
S. Surman-Lee ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Culture Media ◽  
Tap Water ◽  
Clinical Isolates ◽  
Strain Variation ◽  
Liquid Media ◽  
Domestic Water ◽  
Source Of Infection ◽  
Solid Media ◽  
The Impact

Helicobacter pylori is an important global human pathogen and there is growing evidence from PCR assays that contaminated drinking water might be a possible source of infection in some circumstances. There are no validated protocols for direct isolation but various culture media have been developed for possible environmental sampling. Our aim here was to investigate how inter-strain variation might affect the interpretation of results with such media. Two laboratory adapted reference strains and four recent clinical isolates were tested on four solid media and in ten liquid media. Considerable variation was found between strains in their ability to recover on the different media after stress exposure (suspension in sterile tap water). Generally, clinical isolates were less robust than the laboratory-adapted strains and, overall, the former required longer recovery times. Our findings highlighted the importance of using a range of isolates for evaluations, as examination of laboratory-adapted strains alone did not provide an accurate representation of the utility of media that may be used to recover H. pylori from water.

scholarly journals Comparison of Methods for Processing Drinking Water Samples for the Isolation of Mycobacterium avium and Mycobacterium intracellulare

2008 ◽  
Vol 74 (10) ◽  
pp. 3094-3098 ◽  
Author(s):  
Rachel Thomson ◽  
Robyn Carter ◽  
Chris Gilpin ◽  
Chris Coulter ◽  
Megan Hargreaves
Keyword(s):  
Mycobacterium Avium ◽  
Water Samples ◽  
Culture Media ◽  
Tap Water ◽  
Cetylpyridinium Chloride ◽  
Sodium Thiosulfate ◽  
Mycobacterium Intracellulare ◽  
Solid Media ◽  
Lowenstein Jensen ◽  
Mycobacterial Growth

ABSTRACT Several protocols for isolation of mycobacteria from water exist, but there is no established standard method. This study compared methods of processing potable water samples for the isolation of Mycobacterium avium and Mycobacterium intracellulare using spiked sterilized water and tap water decontaminated using 0.005% cetylpyridinium chloride (CPC). Samples were concentrated by centrifugation or filtration and inoculated onto Middlebrook 7H10 and 7H11 plates and Lowenstein-Jensen slants and into mycobacterial growth indicator tubes with or without polymyxin, azlocillin, nalidixic acid, trimethoprim, and amphotericin B. The solid media were incubated at 32°C, at 35°C, and at 35°C with CO2 and read weekly. The results suggest that filtration of water for the isolation of mycobacteria is a more sensitive method for concentration than centrifugation. The addition of sodium thiosulfate may not be necessary and may reduce the yield. Middlebrook M7H10 and 7H11 were equally sensitive culture media. CPC decontamination, while effective for reducing growth of contaminants, also significantly reduces mycobacterial numbers. There was no difference at 3 weeks between the different incubation temperatures.

scholarly journals Production of a bioherbicide agent in liquid and solid medium and in a biphasic cultivation system

2014 ◽  
Vol 32 (2) ◽  
pp. 255-264 ◽  
Author(s):  
C. Moraes ◽  
A.C. Monteiro ◽  
A.C.R. Machado ◽  
J.C. Barbosa ◽  
D.A. Mochi
Keyword(s):  
Large Scale ◽  
Culture Media ◽  
Biphasic System ◽  
Liquid Media ◽  
Control Programs ◽  
Solid Media ◽  
Conidia Production ◽  
Specific Pathogen ◽  
Weeds Control ◽  
Sorghum Grain

The use of fungi in weeds control programs depends upon the conidia production in large scale. Therefore, this study aimed to evaluate liquid and solid culture media and the cultivation by biphasic system for the conidia production of Bipolaris euphorbiae Muchovej & Carvalho a specific pathogen of Euphorbia heterophylla. The liquid media were obtained from agro-industrial waste or by-products, and the solid media were prepared with mixtures of grains and grain derivatives. The liquid medium made with sugar cane molasses stood out from the others because it provided great sporulation (23 x 10(4) conidia mL-1 of medium), conidial viability (99.7%), and formation of mycelial fungal biomass (1.26 g 100 mL-1 of medium). On solid media conidial production was markedly higher than in liquid media, especially the medium composed by a blend of sorghum grain (40%) and soybean hulls (60%) where the fungus produced 2.3 x 10(7) conidia g-1 of medium. The cultivation of B. euphorbiae in biphasic system not promoted a significant increase in the production of conidia. The solid media were more effective for the mass production of fungus and mixtures of grains and derivatives were effective for increasing conidia production.

scholarly journals Evaluation of culture media and conditions of Amazonian filamentous fungi in an antimicrobial screening program

2021 ◽  
Vol 10 (14) ◽  
pp. e370101422065
Author(s):  
Monique Antunes Negreiros ◽  
Milthes Viana Guedes ◽  
Thaís Karoline de Sousa Rodrigues ◽  
Beatriz Rebelo Rodrigues ◽  
Andria Lopes Cruz ◽  
...  
Keyword(s):  
Liquid Medium ◽  
Filamentous Fungi ◽  
Screening Program ◽  
Culture Media ◽  
Coli Strain ◽  
Active Metabolites ◽  
Fermentation Conditions ◽  
Screening Programs ◽  
Solid Media ◽  
The Impact

The rediscovery of bioactive compounds is a problem within natural product screening programs, because the chemical-genetic diversity of fungi is little explored and the standardization of cultivation conditions that allow obtaining new actives is critical in such programs. In this work, the impact of two solid mediums (rice and oats), a liquid medium (Czapeck broth) and different fermentation conditions were evaluated in order to explore new metabolic routes. Twelve filamentous fungi from Amazonian environments were used. UV-Vis spectrophotometry estimated the complexity of the extracts produced. The antimicrobial activity of the extracts was evaluated against an isolate of each Escherichia coli strain, Salmonella sp. and Staphylococcus aureus. Solid media proved to be more promising, as they allowed a wider range of active metabolites to be obtained. The oat medium provided a greater variety of metabolites, but due to the great complexity of the extracts obtained, the separation procedures were considerably more complex than for rice. Together, the rice culture medium and the use of 39 days of fermentation proved to be more promising conditions than the liquid medium normally used in screening programs in Brazil. The cultivation of Penicillium maximae (isolated for the second time in Brazilian territory) in solid medium provided the production of active fractions against E. coli in bioautography. In this study, it was observed that different fermentation conditions in solid culture are considerably promising in the search for bioactive natural products.

scholarly journals Free-living amoebae, Legionella and Mycobacterium in tap water supplied by a municipal drinking water utility in the USA

2009 ◽  
Vol 8 (1) ◽  
pp. 71-82 ◽  
Author(s):  
Francine Marciano-Cabral ◽  
Melissa Jamerson ◽  
Edna S. Kaneshiro
Keyword(s):  
Drinking Water ◽  
Distribution Systems ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
Tap Water ◽  
Light And Electron Microscopy ◽  
Domestic Water ◽  
Free Living ◽  
Water Utility ◽  
The Usa

Legionella and Mycobacterium can proliferate within free-living amoebae (FLA) where they are protected from disinfectants at concentrations that can kill bacteria but not protozoa. Despite effective treatment of drinking water, microbes can enter water utility distribution systems (DS) and hence the plumbing within building premises. Additionally, biofilm formation may account for the persistence of microbes in the DS. In the present study a domestic water tap in north-central United States (USA) was sampled in March and September 2007 and analysed for FLA, Legionella and Mycobacterium. Identification of organisms was determined by growth on specific culture media, light and electron microscopy, and amplification of DNA probes specific for each organism. In both the spring and fall samples, amoebae, Legionella and Mycobacterium were detected. However, Acanthamoeba was prominent in the spring sample whereas Vahlkampfia and Naegleria were the amoebae detected in the autumn. Bacterial proliferation in laboratory cultures was noticeably enhanced in the presence of amoebae and biofilms rapidly formed in mixed amoebae and bacteria cultures. It is hypothesized that temperature affected the dynamics of FLA species population structure within the DS and that pathogenic bacteria that proliferate within FLA, which are themselves opportunistic pathogens, pose dual public health risks.

Study on Fungi Inhabiting Indoor Woods and their Eco-Friendly Management

2016 ◽  
Vol 59 ◽  
pp. 55-61
Author(s):  
Ashwani Tapwal ◽  
Priya Kalyan ◽  
Shailendra Kumar ◽  
Suresh Chandra
Keyword(s):  
Growth Inhibition ◽  
Wood Surface ◽  
Tree Species ◽  
Leaf Extract ◽  
Culture Media ◽  
Maximum Growth ◽  
Liquid Media ◽  
Leaf Extracts ◽  
Solid Media ◽  
The Media

Biodeterioration of indoor wood and microbial pollution inside buildings is one of important problem in humid areas. Number of fungi are associated with indoor wood and many of them are responsible for its deterioration by causing decay and stain. Some of them may release mycotoxins, which have hazardous impact on human health. In present study, fifteen fungi associated with wood surface were isolated and out of which genus Aspergillus dominate with four species. A. flavus was recorded from all locations. The potential of leaf extracts of nine tree species on the growth of A. flavus was evaluated by amending culture media. Considerable growth inhibition of A. flavus in the range of 1.14-45.45% was recorded on solid media and 9.37-86.66% in liquid media. Amendment of culture media @ 30% concentration of the leaf extract of Corymbia torelliana have recorded maximum growth inhibition irrespective of the media used.

Assessment of the Multifactorial Effect on Antimicrobial Sensitivity in Positive Staphylococcus Aureus Clinical Isolates from Assir Region, Saudi Arabia

2012 ◽  
Vol 13 (2) ◽  
pp. 152-159 ◽  
Author(s):  
Nazar M Abdalla ◽  
Waleed O Haimour ◽  
Amani A Osman ◽  
Hassan Abdul Aziz
Keyword(s):  
Staphylococcus Aureus ◽  
Saudi Arabia ◽  
Culture Media ◽  
Meca Gene ◽  
Laboratory Data ◽  
Age Groups ◽  
Clinical Isolates ◽  
Diabetic Patients ◽  
Staph Aureus ◽  
Antimicrobial Sensitivity

General objectives: This study aimed at assessment of factors affecting antimicrobial sensitivity in Staphylococcus aureus clinical isolates from Assir region, Saudi Arabia. Materials and Methods: In this study, eighty one patients presented with Staph. aureus infections either nosocomial or community acquired infections were involved by collecting nasal swabs from them at Aseer Central Hospital General Lab. These patients were from all age groups and from males and females during the period of Jan 2011- Jun 2011. These samples were undergone variable laboratory procedures mainly; bactech, culture media, antibiotics sensitivity test using diffusion disc test (MIC) and molecular (PCR) for detection of mec A gene. Clinical and laboratory data were recorded in special formats and analyzed by statistical computer program (SPSS). Results: Showed that; Descriptive and analytical statistical analysis were performed and final results were plotted in tables. In Staph aureus MecA gene positive cases (50) showed: Oxacillin/ Mithicillin, Ciprofloxacin and Fusidin resistant in diabetic patients were 13, 26.0%, 9, 18% and 7, 14% respectively and in non diabetic patients were 37, 74.0%, 22, 44% and 20, 40% respectively. While no sensitivity in diabetic and non diabetic patients using Oxacillin/ Mithicillin. In Staph aureus MecA gene negative cases (31) showed: Oxacillin/ Mithicillin, sensitivity in diabetic patients (5, 16.1%) and in non diabetic were (26, 83.9%). While no resistant in diabetic and non diabetic patients. In Ciprofloxacin and Fusidin resistant in diabetic patients were 1, 3.2% and 1, 3.2% respectively and in non diabetic patients were 12, 38.7% and 7, 22.6%respectively. Erythromycin in Staph aureus ( MecA gene) positive cases (50) showed: resistant in age (0-15) years were (5, 10%), (16-50) years were (16, 32%) and ( ›50 years) were (12, 24%). Erythromycin in Staph aureus (MecA gene) negative cases (31) showed: resistant in age (0-15) years were (6, 19.3%), (16-50) years were (5, 16.1%) and ( ›50 years) were (3, 9.7%). Conclusion: Drugs resistance is a major progressive multifactorial problem facing the treatment of Staph aureus infections. DOI: http://dx.doi.org/10.3329/jom.v13i2.12750 J Medicine 2012; 13 : 152-159

Impact of Particulate Matter on Distribution System Disinfection Efficacy

2008 ◽  
Vol 43 (1) ◽  
pp. 55-62 ◽  
Author(s):  
Linda Wojcicka ◽  
Carole Baxter ◽  
Ron Hofmann
Keyword(s):  
Helicobacter Pylori ◽  
Particulate Matter ◽  
Distribution System ◽  
Contact Time ◽  
Water Disinfection ◽  
Sphingomonas Paucimobilis ◽  
Soil Corrosion ◽  
Log Reduction ◽  
Drinking Water Disinfection ◽  
The Impact

Abstract Microorganisms have been shown to survive drinking water disinfection and remain viable in disinfected waters despite the presence of disinfectant residuals. This may be partially attributed to protection by particulate matter. The aim of this study was to determine the effects of the presence of particulate matter on disinfection kinetics. Sphingomonas paucimobilis ATCC 10829 and Helicobacter pylori ATCC 43504 were used in inactivation experiments in the presence and absence of soil, corrosion, and wastewater particles. The results showed that the presence of such particles tended to inhibit chlorine and monochloramine inactivation, although the magnitude of the impact under the conditions tested was small (e.g., 1-log reduction in inactivation for several minutes of contact time in the presence of less than 1 mg/L of disinfectant).

scholarly journals Polyphenol Profiling of Chestnut Pericarp, Integument and Curing Water Extracts to Qualify These Food By-Products as a Source of Antioxidants

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2335
Author(s):  
Gabriella Pinto ◽  
Sabrina De Pascale ◽  
Maria Aponte ◽  
Andrea Scaloni ◽  
Francesco Addeo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Negative Impact ◽  
Tap Water ◽  
Time Of Flight ◽  
The Other ◽  
Curing Process ◽  
Flight Mass Spectrometry ◽  
Water Curing ◽  
The Impact ◽  
By Products

Plant polyphenols have beneficial antioxidant effects on human health; practices aimed at preserving their content in foods and/or reusing food by-products are encouraged. The impact of the traditional practice of the water curing procedure of chestnuts, which prevents insect/mould damage during storage, was studied to assess the release of polyphenols from the fruit. Metabolites extracted from pericarp and integument tissues or released in the medium from the water curing process were analyzed by matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS) and electrospray-quadrupole-time of flight-mass spectrometry (ESI-qTOF-MS). This identified: (i) condensed and hydrolyzable tannins made of (epi)catechin (procyanidins) and acid ellagic units in pericarp tissues; (ii) polyphenols made of gallocatechin and catechin units condensed with gallate (prodelphinidins) in integument counterparts; (iii) metabolites resembling those reported above in the wastewater from the chestnut curing process. Comparative experiments were also performed on aqueous media recovered from fruits treated with processes involving: (i) tap water; (ii) tap water containing an antifungal Lb. pentosus strain; (iii) wastewater from a previous curing treatment. These analyses indicated that the former treatment determines a 6–7-fold higher release of polyphenols in the curing water with respect to the other ones. This event has a negative impact on the luster of treated fruits but qualifies the corresponding wastes as a source of antioxidants. Such a phenomenon does not occur in wastewater from the other curing processes, where the release of polyphenols was reduced, thus preserving the chestnut’s appearance. Polyphenol profiling measurements demonstrated that bacterial presence in water hampered the release of pericarp metabolites. This study provides a rationale to traditional processing practices on fruit appearance and qualifies the corresponding wastes as a source of bioactive compounds for other nutraceutical applications.

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