Detection and identification of dyes showing AhR–binding affinity in treated sewage effluents

2006 ◽  
Vol 53 (11) ◽  
pp. 35-42 ◽  
Author(s):  
P.-H. Chou ◽  
S. Matsui ◽  
T. Matsuda
Keyword(s):  
Rhodamine B ◽  
Reversed Phase ◽  
Future Research ◽  
Acid Dyes ◽  
Anthraquinone Dyes ◽  
Chemical Structures ◽  
Sewage Effluents ◽  
Treated Sewage ◽  
Recombinant Yeast Strain ◽  
Ligand Activity

A bioassay using the YCM3 recombinant yeast strain was utilised to investigate the presence of dioxin-like compounds that activate the aryl hydrocarbon receptor (AhR) in treated sewage effluents. AhR ligand activity was detected in the concentrated extracts of effluent samples collected in March, June and October 2004 from Kyoto city, Japan. HPLC fractionation was carried out using C18 reversed-phase columns, and possible AhR ligands were further isolated and purified. By using LC/MS/MS, one weak AhR ligand was identified to be rhodamine B base, a fluoran dye. In addition, two other coloured ligands were postulated to be disperse anthraquinone dyes or their metabolites because of their UV spectra and HPLC retention times. The AhR-binding affinities of 12 commercial dyes with different chemical structures were also studied. Among the dyes tested, hydrophobic anthraquinone dyes exhibited higher AhR ligand activity, but azo dyes or hydrophilic acid dyes showed no or very low AhR ligand activity. Rhodamine B base and disperse anthraquinone dyes were suggested to be potential xenobiotic AhR ligands. Future research regarding their contamination in aquatic environments and toxicological information is necessary.

Contamination of polycyclic aromatic hydrocarbons and their implication for environmental hazard

2006 ◽  
Vol 6 (6) ◽  
pp. 165-174
Author(s):  
P.-H. Chou ◽  
S. Matsui
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
River System ◽  
Point Sources ◽  
Anthraquinone Dyes ◽  
Sources Of Pollution ◽  
Sewage Effluents ◽  
Treated Sewage ◽  
Gene Assay ◽  
Polycyclic Aromatic

Studies concerning the contamination and pollution sources of polycyclic aromatic hydrocarbons (PAHs) in the Yodo River System, Japan, are summarized in this review. Mutagenicity and genotoxicity have been frequently detected in the tributary rivers of Yodo River, and novel contaminants such as phenylbenzotriazole type mutagens, nitrated PAHs, and heterocyclic amines have been suggested to be the causative agents. The contribution of nonsubstituted PAHs to the toxicity is considered to be low since only few ng/l of phenanthrene, fluoranthene, and pyrene have been found in the river waters. The application of an aryl hydrocarbon receptor (AhR) dependent bioassay in evaluating the combined toxicity caused by various pollutants in environmental samples is also introduced. Point and non-point sources of pollution discharging into the Lake Biwa-Yodo River System, including treated sewage effluents and deposited road particles, have been investigated using the AhR-dependent reporter gene assay. The results suggested the presence of newly identified AhR ligands such as anthraquinone dyes or unidentified AhR ligands in the treated sewage effluents and the road particles, respectively. Finally, toxic effects of PAHs that are mediated via AhR binding are also summarized.

Pharmacological Uses of the Plants Belonging to the Genus Commiphora

2020 ◽  
Vol 18 ◽  
Author(s):  
Subbiah Latha ◽  
Palanisamy Selvamani ◽  
Thangavelu Prabha
Keyword(s):  
Secondary Metabolites ◽  
Plant Species ◽  
Dna Cleavage ◽  
Structural Information ◽  
Chemical Constituents ◽  
Future Research ◽  
Pharmacological Properties ◽  
Chemical Structures ◽  
Indigenous Uses ◽  
Available Information

: Natural products have a unique place in the healthcare industry. The genus Commiphora emerged as a potential medicinal with huge benefits as evidenced through its use in various traditional and modern systems of medicine. Therefore, we aimed to prepare a concise review on the pharmacological activities and the indigenous uses of various plant species belonging to the genus Commiphora along with the structural information of various active botanical ingredients present in these plants based on the published literatures and scientific reports. To collect the various published literatures on Commiphora in various journals; to study and classify the available information on the pharmacological uses and chemical constituents; and to present the gathered information as a precise review to serve as a potential reference for future research. Pharmacological and phytochemical data on Commiphora plant species were collected from various journals, books, reference materials, websites including scientific databases, etc for compilation. This review article describes the various pharmacological properties of plants of Commiphora species viz., Anti-arthritic and anti-inflammatory, Anti-atherogenic, Antibacterial, Anti-coagulant, Anti-dicrocoeliasis, Anti-epileptic, Anti-fascioliasis, Anti-fungal, Anti-heterophyidiasis, Anti-hyper cholesterolemic, Anti-hyperlipidemic, Anti-hypothyroidism, Anti-obesity, Anti-osteoarthritic, Anti-osteoclastogenesis, Anti-oxidant, Anti-parasitic, Anti-pyretic, Anti-schistosomiasis, Anti-septic, Anti-thrombotic, Anti-ulcer, Cardioprotective, COX enzyme inhibitory, Cytotoxic /Anti-carcinogenic/Anti-cancer, DNA cleavage, Hypotensive, Inhibits lipid peroxidation, Inhibits NO and NO synthase production, Insecticidal, Local anesthetic, Molluscicidal, Smooth muscle relaxant, Tick repellent activities along with toxicity studies. Furthermore, the review also included various secondary metabolites isolated from various species of Commiphora genus along with their chemical structures serve as a ready resource for researchers. We conclude that the plant species belonging to the genus Commiphora possesses abundant pharmacological properties with a huge treasure of diverse secondary metabolites within themselves. This review indicates the necessity of further in-depth research, pre-clinical and clinical studies with Commiphora genus which may help to detect the unidentified potential of the Commiphora plant species.

Investigation of the effects of silicone emulsions having different particle sizes on knitted fabrics depending on the type of yarn

2017 ◽  
Vol 29 (3) ◽  
pp. 394-416 ◽  
Author(s):  
Burcu Sancar Besen ◽  
Onur Balci
Keyword(s):  
Future Research ◽  
Particle Sizes ◽  
Knitted Fabrics ◽  
Content Type ◽  
Bursting Strength ◽  
Chemical Structures ◽  
Strength And Stiffness ◽  
Ring Open ◽  
Working Parameters ◽  
Physical And Chemical

Purpose The purpose of this paper is to investigate the effects of silicone-based softeners, which were developed with different particle sizes (nano, micro, and macro) and chemical structures, on the performance of 100 percent cotton fabrics knitted with different type of yarn (ring, open-end, and compact). Design/methodology/approach In the study, the silicone emulsions having expected particle sizes were produced at laboratory conditions. The produced silicone emulsions were applied to knitted fabrics with both padding and exhaust methods at different concentrations. Some characterization tests (particle size and zeta potential) were applied to the silicone emulsions before the applications. After the applications, CIELab values, whiteness and color fastness, hyrophility, abrasion, pilling, bursting strength, and stiffness performances of the samples were tested. The changes of the investigated properties were also examined via ANOVA. Findings According to the results, it was found that the silicone applications caused the CIELab values, whiteness degree, hyrophility, pilling, bursting strength and stiffness performance of the fabrics to change depending on the particle sizes of the emulsions, the yarn type of the fabrics, the application type, and the concentration of the silicone emulsions. When the ANOVA results were examined, it was seen that the types of the yarn and the silicone emulsions were the most effective working parameters on the results. Research limitations/implications Because no additives were added to the produced silicone emulsions, in the future research, they can be developed with the use of some additives. Thus, it can resolve some of the disadvantages of the silicone emulsions on the textiles. Practical implications While applying the silicone softeners to the knitted fabrics, the type of the yarn and the particle sizes of the emulsions must be determined according to each other, in order to obtain enough handle performance without causing negative change on the other important properties of the knitted fabrics. Originality/value When the studies regarding silicone softeners were investigated, it was found that there were no studies about the effect of the silicone softeners having different particle sizes on the physical and chemical structures of the knitted fabrics depending on the type of yarn and some working parameters such as concentration and type of the application.

Bait uptake by free living brush-tailed phascogales Phascogale tapoatafa and other non-target mammals during simulated buried fox baiting.

2003 ◽  
Vol 25 (1) ◽  
pp. 31 ◽  
Author(s):  
D Fairbridge ◽  
R Anderson ◽  
T Wilkes ◽  
G Pell
Keyword(s):  
Small Mammals ◽  
Rhodamine B ◽  
Field Trials ◽  
Future Research ◽  
Research Needs ◽  
Trichosurus Vulpecula ◽  
Free Living ◽  
Central Victoria ◽  
Petaurus Breviceps

Field trials were undertaken in box-ironbark woodland at Puckapunyal Military Area (PMA) in central Victoria between January 2000 and April 2001 to assess bait uptake by the brushtailed phascogale (Phascogale tapoatafa) and other small mammals during simulated fox baiting exercises. The systemic marker Rhodamine B was used in non-toxic fox baits (Foxoff®) to detect non-target bait consumption. The trials demonstrated that free-living brush-tailed phascogales, yellow-footed antechinus (Antechinus flavipes), sugar gliders (Petaurus breviceps) and common brushtail possums (Trichosurus vulpecula) were capable of accessing non-toxic fox baits buried under 10 cm of sand. Rhodamine B markings were detected in six (15%) of 40 P. tapoatafa captured during the study period. The implications of these results and future research needs are discussed.

scholarly journals Advances in the Dereplication of Aroma Precursors from Grape Juice by Pretreatment with Lead Acetate and Combined HILIC- and RP-HPLC Methods

Foods ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 28
Author(s):  
Michele D’Ambrosio
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Grape Juice ◽  
2D Nmr ◽  
Reversed Phase ◽  
Natural Form ◽  
Chemical Structures ◽  
Rp Hplc ◽  
2D Nmr Spectra ◽  
Aroma Precursors

Glycosidic aroma precursors (GAPs) contribute to the varietal flavor of wine. Researchers have applied various sample preparation and analytical methods in attempts to achieve their separation and identification. However, mass spectrometric methods still fail to unequivocally define their structures. We have previously reported the separation of GAPs in their natural form and elucidated their structures by nuclear magnetic resonance (NMR) spectroscopy. In this study, we confirm the effectiveness of our established procedure and present methodological improvements. Grape juice was treated with lead (II) acetate and repeatedly chromatographed to give seven pure GAPs. Their chemical structures were characterized by MSn fragmentations and 1D- and 2D-NMR spectra. Ten GAPs were analyzed by both hydrophilic interaction liquid chromatography (HILIC) and reversed phase high performance liquid chromatography (RP-HPLC) to compare the two chromatograms. A selection of known phenols was treated with lead (II) acetate in order to check its binding properties.

scholarly journals Sulfated Glycans and Related Digestive Enzymes in the Zika Virus Infectivity: Potential Mechanisms of Virus-Host Interaction and Perspectives in Drug Discovery

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Vitor H. Pomin
Keyword(s):  
Neural Development ◽  
Zika Virus ◽  
Molecular Mechanisms ◽  
Surface Proteins ◽  
Host Cells ◽  
Future Research ◽  
Virus Infectivity ◽  
Virus Attachment ◽  
Chemical Structures ◽  
Host Interaction

As broadly reported, there is an ongoing Zika virus (ZIKV) outbreak in countries of Latin America. Recent findings have demonstrated that ZIKV causes severe defects on the neural development in fetuses in utero and newborns. Very little is known about the molecular mechanisms involved in the ZIKV infectivity. Potential therapeutic agents are also under investigation. In this report, the possible mechanisms of action played by glycosaminoglycans (GAGs) displayed at the surface proteoglycans of host cells, and likely in charge of interactions with surface proteins of the ZIKV, are highlighted. As is common for the most viruses, these sulfated glycans serve as receptors for virus attachment onto the host cells and consequential entry during infection. The applications of (1) exogenous sulfated glycans of different origins and chemical structures capable of competing with the virus attachment receptors (supposedly GAGs) and (2) GAG-degrading enzymes able to digest the virus attachment receptors on the cells may be therapeutically beneficial as anti-ZIKV. This communication attempts, therefore, to offer some guidance for the future research programs aimed to unveil the molecular mechanisms underlying the ZIKV infectivity and to develop therapeutics capable of decreasing the devastating consequences caused by ZIKV outbreak in the Americas.

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