Molecular biological methods (DGGE) as a tool to investigate nitrification inhibition in wastewater treatment

2003 ◽  
Vol 47 (11) ◽  
pp. 165-172 ◽  
Author(s):  
N. Kreuzinger ◽  
A. Farnleitner ◽  
G. Wandl ◽  
R. Hornek ◽  
R. Mach
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Full Scale ◽  
Two Stage ◽  
Plant Laboratory ◽  
Biological Methods

Incomplete nitrification at an activated sludge plant for biological pre-treatment of rendering plant effluents led to a detailed investigation on the origin and solution of this problem. Preliminary studies revealed that an inhibition of ammonia oxidising microorganisms (AOM) by process waters of the rendering plant was responsible for the situation. We were able to show a correlation between the existence of specific AOM and nitrification capacity expressed as oxygen uptake rate for maximal nitrification (OURNmax). Only Nitrosospira sp. was found in the activated sludge of the rendering plant and another industrial wastewater treatment plant with problems in nitrification, while reference plants without nitrification problems showed Nitrosomonas spp. as the predominant ammonia oxidising bacteria. By accompanying engineering investigations and experiments (cross-feeding experiments, operation of a two-stage laboratory plant) with molecular biological methods (DGGE - Denaturing Gradient Gel Electrophoresis) we were able to elaborate an applicable solution for the rendering plant. Laboratory experiments with a two-stage process layout finally provided complete nitrification overcoming the inhibiting nature of process waters from the rendering plant. DGGE analysis of the second stage activated sludge from the laboratory plant showed a shift in population structure from Nitrosospira sp. towards Nitrosomonas spp. simultaneous to the increase of nitrification capacity. Nitrification capacities comparable to full-scale municipal wastewater treatment plants could be maintained for more than two months. As the design of wastewater treatment plants for nitrification is linked to the growth characteristics of Nitrosomonas spp., established criteria can be applied for the redesign of the full-scale plant.

scholarly journals LCA of a Membrane Bioreactor Compared to Activated Sludge System for Municipal Wastewater Treatment

Membranes ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 421
Author(s):  
Dimitra C. Banti ◽  
Michail Tsangas ◽  
Petros Samaras ◽  
Antonis Zorpas
Keyword(s):  
Wastewater Treatment ◽  
Life Cycle ◽  
Activated Sludge ◽  
Membrane Bioreactor ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Real Data ◽  
Municipal Wastewater Treatment ◽  
Conventional Activated Sludge

Membrane bioreactor (MBR) systems are connected to several advantages compared to the conventional activated sludge (CAS) units. This work aims to the examination of the life cycle environmental impact of an MBR against a CAS unit when treating municipal wastewater with similar influent loading (BOD = 400 mg/L) and giving similar high-quality effluent (BOD < 5 mg/L). The MBR unit contained a denitrification, an aeration and a membrane tank, whereas the CAS unit included an equalization, a denitrification, a nitrification, a sedimentation, a mixing, a flocculation tank and a drum filter. Several impact categories factors were calculated by implementing the Life Cycle Assessment (LCA) methodology, including acidification potential, eutrophication potential, global warming potential (GWP), ozone depletion potential and photochemical ozone creation potential of the plants throughout their life cycle. Real data from two wastewater treatment plants were used. The research focused on two parameters which constitute the main differences between the two treatment plants: The excess sludge removal life cycle contribution—where GWPMBR = 0.50 kg CO2-eq*FU−1 and GWPCAS = 2.67 kg CO2-eq*FU−1 without sludge removal—and the wastewater treatment plant life cycle contribution—where GWPMBR = 0.002 kg CO2-eq*FU−1 and GWPCAS = 0.14 kg CO2-eq*FU−1 without land area contribution. Finally, in all the examined cases the environmental superiority of the MBR process was found.

scholarly journals Initiating Biodegradation of Polyvinylpyrrolidone in an Aqueous Aerobic Environment: Technical Note / Zainicjowanie Biodegradacji Poliwinylopirolidonu W Środowisku Wodno-Tlenowym: Notatki Techniczne

2013 ◽  
Vol 20 (1) ◽  
pp. 199-208 ◽  
Author(s):  
Marketa Julinova ◽  
Jan Kupec ◽  
Roman Slavik ◽  
Maria Vaskova
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Waste Treatment ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Oxygen Demand ◽  
Technical Note ◽  
Aerobic Biodegradation ◽  
Municipal Wastewater Treatment

Abstract A synthetic polymer, polyvinylpyrrolidone (PVP - E 1201) primarily finds applications in the pharmaceutical and food industries due to its resistance and zero toxicity to organisms. After ingestion, the substance passes through the organism unchanged. Consequently, it enters the systems of municipal wastewater treatment plants (WWTP) without decomposing biologically during the waste treatment process, nor does it attach (through sorption) to particles of activated sludge to any significant extent, therefore, it passes through the system of a WWTP, which may cause the substance to accumulate in the natural environment. For this reason the paper investigates the potential to initiate aerobic biodegradation of PVP in the presence of activated sludge from a municipal wastewater treatment plant. The following agents were selected as the initiators of the biodegradation process - co-substrates: acrylamide, N-acethylphenylalanine and 1-methyl-2-pyrrolidone, a substance with a similar structure to PVP monomer. The biodegradability of PVP in the presence of co-substrates was evaluated on the basis of biological oxygen demand (BOD) as determined via a MicroOxymax O2/CO2/CH4 respirometer. The total substrate concentration in the suspension equaled 400 mg·dm-3, with the ratio between PVP and the cosubstrate being 1:1, while the concentration of the dry activated sludge was 500 mg·dm-3. Even though there was no occurrence of a significant increase in the biodegradation of PVP alone in the presence of a co-substrate, acrylamide appeared to be the most effective type of co-substrate. Nevertheless, a recorded decrease in the slope of biodegradation curves over time may indicate that a process of primary decomposition was underway, which involves the production of metabolites that inhibit activated sludge microorganisms. The resulting products are not identified at this stage of experimentation.

scholarly journals Activated Sludge Microbial Community and Treatment Performance of Wastewater Treatment Plants in Industrial and Municipal Zones

2020 ◽  
Vol 17 (2) ◽  
pp. 436 ◽  
Author(s):  
Yongkui Yang ◽  
Longfei Wang ◽  
Feng Xiang ◽  
Lin Zhao ◽  
Zhi Qiao
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Wastewater Sludge ◽  
Community Diversity ◽  
Nitrogen And Phosphorus ◽  
Metabolic Functions

Controlling wastewater pollution from centralized industrial zones is important for reducing overall water pollution. Microbial community structure and diversity can adversely affect wastewater treatment plant (WWTP) performance and stability. Therefore, we studied microbial structure, diversity, and metabolic functions in WWTPs that treat industrial or municipal wastewater. Sludge microbial community diversity and richness were the lowest for the industrial WWTPs, indicating that industrial influents inhibited bacterial growth. The sludge of industrial WWTP had low Nitrospira populations, indicating that influent composition affected nitrification and denitrification. The sludge of industrial WWTPs had high metabolic functions associated with xenobiotic and amino acid metabolism. Furthermore, bacterial richness was positively correlated with conventional pollutants (e.g., carbon, nitrogen, and phosphorus), but negatively correlated with total dissolved solids. This study was expected to provide a more comprehensive understanding of activated sludge microbial communities in full-scale industrial and municipal WWTPs.

scholarly journals SARS-CoV-2 Detection in Istanbul Wastewater Treatment Plant Sludges

2020 ◽  
Author(s):  
Bilge Alpaslan Kocamemi ◽  
Halil Kurt ◽  
Ahmet Sait ◽  
Fahriye Sarac ◽  
Ahmet Mete Saatci ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Waste Activated Sludge ◽  
Secondary Process ◽  
Virus Concentration ◽  
Municipal Wastewater Treatment ◽  
Primary Sludge

Following the announcement of SARS-CoV-2 worldwide pandemic spread by WHO on March 11, 2020, wastewater based epidemiology received great attention in several countries: The Netherlands [Medama et al., 2020; K-Lodder et al., 2020], USA [Wu et al., 2020; Memudryi et al., 2020], Australia [Ahmed et al., 2020], France [Wurtzer et al., 2020], China [Wang et al., 2020], Spain [Randazzo et al., 2020; Walter et al., 2020], Italy (La Rosa et al., 2020; Rimoldi et al., 2020) and Israel [Or et al., 2020], performed analysis in wastewaters by using different virus concentration techniques. Turkey took its place among these countries on 7th of May, 2020 by reporting SARS-CoV-2 RT-qPCR levels at the inlet of seven (7) major municipal wastewater treatment plants (WWTPs) of Istanbul [Alpaslan Kocamemi et al., 2020], which is a metropole with 15.5 million inhabitants and a very high population density (2987 persons/km2) and having about 65 % of Covid-19 cases in Turkey. Sludges that are produced in WWTPs should be expected to contain SARS-CoV-2 virus as well. There has not yet been any study for the fate of SAR-CoV-2 in sludges generated from WWTPs. Knowledge about the existing of SARS-CoV-2 in sludge may be useful for handling the sludge during its dewatering, stabilizing and disposal processes. This information will also be valuable in case of sludges that are used as soil conditioners in agriculture or sent to landfill disposal. In wastewater treatment plants, generally two different types of sludges are generated; primary sludge (PS) and waste activated sludge (WAS). PS forms during the settling of wastewater by gravity in the primary settling tanks. Little decomposition occurs during primary sludge formation. Since most of the inorganic part of the wastewater is removed in the earlier grit removal process, the PS consists of mainly organic material that settles. The PS is about 1-2 % solids by weight. In the biological treatment part of the WWTPs, the biomass that forms in the anaerobic, anoxic and oxic zones of the process is settled in final clarifiers by gravity and returned to the beginning of the biological process so that it is not washed off. The waste activated sludge (WAS) is the excess part of the biomass that grows in this secondary treatment process. It has to be removed from the process not to increase the mixed liquor suspended solids concentration (bacteria concentration) in the secondary process more than a fixed value. The WAS is about 0.6 - 0.9 % solids by weight. This work aims to find whether SARS-CoV-19 is present in the PS and WAS before it is dewatered and sent to anaerobic or aerobic digester processes or to thermal drying operations. For this purpose, on the 7th of May 2020, two (2) PS samples were collected from Ambarlı and Tuzla WWTPs, seven (7) WAS samples were collected from Terkos, Ambarlı, Atakoy I & II, Pasakoy II, Buyukcekmece and Tuzla I WWTPs. Polyethylene glycol 8000 (PEG 8000) adsorption [Wu et al., 2020] SARS-Cov-2 concentration method was used for SARS-CoV-2 concentration after optimization. [Alpaslan Kocamemi et al., 2020]. Real time RT-PCR diagnostic panel validated by US was used to quantify SARS-CoV-2 RNA in primary and waste activated sludge samples taken from WWTPs in Istanbul. All samples were tested positive. Titers of SARS-CoV-2 have been detected ranging copies between 1.17E4 to 4.02x104 per liter.

The main wastewater treatment plant of Vienna: an example of cost effective wastewater treatment for large cities

2006 ◽  
Vol 54 (10) ◽  
pp. 79-86 ◽  
Author(s):  
G. Wandl ◽  
H. Kroiss ◽  
K. Svardal
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Nitrogen Removal ◽  
Nutrient Removal ◽  
Process Management ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Single Stage ◽  
Volume Index ◽  
Two Stage

Two-stage activated sludge plants succeed in stable treatment efficiency concerning carbon removal and nitrification with far less reactor tank volume than conventional single stage systems. In case of large treatment plants this fact is of great economic relevance. Because of the very small specific volume of these two-stage treatment plants in comparison with low loaded single-stage plants, internal cycles have to be applied to ensure sufficient nitrogen removal. Due to these internal cycles two stage activated sludge plants offer many possibilities in terms of process management which results in new process optimisation procedures as compared to conventional single-stage nutrient removal treatment plants. The proposed extension concept for the Main Treatment Plant of Vienna was validated with pilot plant investigations especially with regard to nitrogen removal where it proved to comply with the legal requirements. The operation of the treatment plant can easily be adapted to changes in temperature and sludge volume index occurring in full scale practice. Sludge retention time and aerobic volume in the second stage are controlled in order to secure sufficient nitrification capacity and to optimise nitrogen removal by means of the variation of the loading conditions for the two stages. The investigations confirmed that the specific two-stage activated sludge concept applied in Vienna is an economically advantageous alternative for large wastewater treatment plants with stringent requirements for nitrification and nutrient removal.

scholarly journals Causes and remedies for filamentous foaming in activated sludge treatment plant

2014 ◽  
Vol 16 (4) ◽  
pp. 762-772 ◽  
Keyword(s):  
Activated Sludge ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Mycolic Acid ◽  
Foam Formation ◽  
Activated Sludge Process ◽  
Polymer Addition ◽  
Physical Chemical ◽  
Biological Methods

<p>This paper reviews the problem of foaming associated with the activated sludge process and its control using various physical, chemical and biological methods. Activated sludge process is widely used for treatment of every type of wastewater like industrial, domestic and municipal wastewater. This process is driven by a complex microbial population, among which some mycolic acid containing bacteria leads to the stable foam formation which ultimately results in poor efficiency of the plants and leading to major environmental, operational, and health problems. A number of researches provide the evidences of foaming in wastewater treatment plants and its control using physical, chemical and biological methods. Current approaches for controlling foam includes operational adjustments, additional structures, controlling dissolved oxygen levels, water sprays, steam application, polymer addition, chlorination and a novel and ecofriendly approach that is treatment of filamentous bacteria with the specific phages. A detailed study of all methods is presented and collectively described in this review paper for a better understanding of the foam controlling strategies.&nbsp;</p>

Influence of temperature and sludge loading on activated sludge settling, especially on Microthrix parvicella

1998 ◽  
Vol 37 (4-5) ◽  
pp. 27-35 ◽  
Author(s):  
S. Knoop ◽  
S. Kunst
Keyword(s):  
Wastewater Treatment ◽  
Low Temperature ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Loading Rate ◽  
Wastewater Treatment Plants ◽  
Full Scale ◽  
Causative Organism ◽  
Foam Formation ◽  
Microthrix Parvicella

During recent years modern full scale wastewater treatment plants with biological nitrification, denitrification and phosphorus removal have had increasing problems with foam formation on the surfaces of aerobic tanks and with bulking activated sludge. The results of a survey in 1995 (Kunst and Knoop, 1996) showed that most often the filamentous bacterium Microthrix parvicella is responsible for these problems. Up to today there is only little knowledge about its selection criteria in activated sludge. Therefore several experiments were done in full scale activated sludge plants and in laboratory systems under defined conditions to investigate the influence of low (&lt; 0.1 kg/(kg·d)) and high (≤ 0.2 kg/(kg·d)) BOD5-sludge loading rates on the growth and morphology of M. parvicella and the settlement of activated sludge. Furthermore the influence of temperatures of 5°C, 12°C and 20°C on the growth of M. parvicella was investigated. It was shown that M. parvicella grows at low BOD5-sludge loading rate and low temperature and is the main causative organism of bulking and foaming sludge in nutrient removal plants. On the basis of this investigation it was concluded that the growth of M. parvicella and the settling problems of the activated sludge resulting from excessive growth of this filament will always appear in modern municipal wastewater treatment plants with BOD5-sludge loading rate ≤ 0.1 kg/(kg·d) especially under low temperature conditions.

Benefits and drawbacks of thermal pre-hydrolysis for operational performance of wastewater treatment plants

2008 ◽  
Vol 58 (8) ◽  
pp. 1547-1553 ◽  
Author(s):  
P. Phothilangka ◽  
M. A. Schoen ◽  
B. Wett
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Biogas Production ◽  
Treatment Plant ◽  
Full Scale ◽  
Plant Performance ◽  
Ammonia Release ◽  
Hydrolysis Process ◽  
Solids Content

This paper presents benefits and potential drawbacks of thermal pre-hydrolysis of sewage sludge from an operator's prospective. The innovative continuous Thermo-Pressure-Hydrolysis Process (TDH) has been tested in full-scale at Zirl wastewater treatment plant (WWTP), Austria, and its influence on sludge digestion and dewatering has been evaluated. A mathematical plant-wide model with application of the IWA Activated Sludge Model No.1 (ASM1) and the Anaerobic Digestion Model No.1 (ADM1) has been used for a systematic comparison of both scenarios—operational plant performance with and without thermal pre-hydrolysis. The impacts of TDH pre-hydrolysis on biogas potential, dewatering performance and return load in terms of ammonia and inert organic compounds (Si) have been simulated by the calibrated model and are displayed by Sankey mass flow figures. Implementation of full scale TDH process provided higher anaerobic degradation efficiency with subsequent increased biogas production (+75–80%) from waste activated sludge (WAS). Both effects—enhanced degradation of organic matter and improved cake's solids content from 25.2 to 32.7% TSS—promise a reduction in sludge disposal costs of about 25%. However, increased ammonia release and generation of soluble inerts Si was observed when TDH process was introduced.

Sign in / Sign up

Export Citation Format

Share Document