Quantitative estimation of the role of denitrifying phosphate accumulating organisms in nutrient removal

2003 ◽  
Vol 47 (11) ◽  
pp. 23-29 ◽  
Author(s):  
T. Shoji ◽  
H. Satoh ◽  
T. Mino

It has been reported that a proportion of polyphosphate-accumulating organisms (PAOs) can denitrify or utilize nitrate as an electron acceptor. The usage of denitrifying-PAO (DN-PAO) can relieve the competition for COD between denitrification and phosphorus removal because they can treat nitrate and phosphate by using the same carbon source. To effectively use DN-PAO for biological nutrient removal (BNR), a new system was proposed in which an anaerobic phase is placed at the influent end, followed by the anoxic and external nitrification phase. In this study, the lab-scale proposed system (A2N system) was operated with a municipal wastewater 1) to confirm stable settlement of DN-PAO in the proposed system, 2) to quantitatively estimate the fraction of different groups of organisms like denitrifiers without polyphosphate accumulating capability, aerobic-PAO and DN-PAO and 3) to estimate the advantages of DN-PAO's presence in the system. Moreover, batch experiments in which anoxic and aerobic phosphate uptake rates (PUR) were measured were also carried out. The activity of DN-PAO was observed throughout the experimental period by the batch experiment. From the results of the calculation of COD utilized by each group of organisms, it was concluded that the proposed system could accumulate much more PAO (as DN-PAO) than conventional BNR systems. Moreover, they were responsible for both EBPR and denitrification.

2006 ◽  
Vol 53 (9) ◽  
pp. 169-175 ◽  
Author(s):  
K. Hamada ◽  
T. Kuba ◽  
V. Torrico ◽  
M. Okazaki ◽  
T. Kusuda

A shortage of organic substances (COD) may cause problems for biological nutrient removal, that is, lower influent COD concentration leads to lower nutrient removal rates. Biological phosphorus removal and denitrification are reactions in which COD is indispensable. As for biological simultaneous nitrogen and phosphorus removal systems, a competition problem of COD utilisation between polyphosphate accumulating organisms (PAOs) and non-polyphosphate-accumulating denitrifiers is not avoided. From the viewpoint of effective utilisation of limited influent COD, denitrifying phosphorus-removing organisms (DN-PAOs) can be effective. In this study, DN-PAOs activities in modified UCT (pre-denitrification process) and DEPHANOX (post-denitrification ptocess) wastewater treatments were compared. In conclusion, the post-denitrification systems can use influent COD more effectively and have higher nutrient removal efficiencies than the conventional pre-denitrification systems.


1998 ◽  
Vol 37 (9) ◽  
pp. 219-226 ◽  
Author(s):  
Choi Euiso ◽  
Rhu Daewhan ◽  
Yun Zuwhan ◽  
Lee Euisin

The wastewater characteristics of low organic strength coupled with low temperature would be considerable variables for design and operation of biological nutrient removal (BNR) systems. But temperature studies have mostly been focused on individual process with biological phosphorus removal, nitrification and denitrification, respectively. Overall temperature effects on BNR system may not be fully represented by sum of results of separated studies on biological nutrient removal steps. The operating result of a retrofitted full scale unit along with laboratory-scale BNR unit indicated 90% of nitrification was possible at temperature as low as 8°C. However, the denitrification was turned out to be a key step to regulate the overall nutrient removal efficiencies. When the operating temperature dropped down, a rapid decrease of phosphorus removal efficiencies was observed by the nitrate in return sludge. If nitrification was not well developed, phosphorus removal returned to the normal efficiency even at low temperature of 5°C. The phosphorus removal mechanism was not influenced at this low temperature.


Author(s):  
Jakub Drewnowski ◽  
Jacek Makinia ◽  
Lukasz Kopec ◽  
Francisco-Jesus Fernandez-Morales

The biodegradation of particulate substrates starts by a hydrolytic stage. Hydrolysis is a slow reaction and usually becomes the rate limiting step of the organic substrates biodegradation. The objective of this work was to evaluate a novel hydrolysis concept based on a modification of the activated sludge model (ASM2d) and to compare it with the original ASM2d model. The hydrolysis concept was developed in order to accurately predict the use of internal carbon sources in enhanced biological nutrient removal (BNR) processes at a full scale facility located in northern Poland. Both hydrolysis concepts were compared based on the accuracy of their predictions for the main processes taking place at a full-scale facility. From the comparison, it was observed that the modified ASM2d model presented similar predictions to those of the original ASM2d model on the behavior of chemical oxygen demand (COD), NH4-N, NO3-N, and PO4-P. However, the modified model proposed in this work yield better predictions of the oxygen uptake rate (OUR) (up to 5.6 and 5.7%) as well as in the phosphate release and uptake rates.


1997 ◽  
Vol 36 (12) ◽  
pp. 61-68 ◽  
Author(s):  
Eun Lee Sang ◽  
Soo Kim Kwang ◽  
Hwan Ahn Jae ◽  
Whoe Kim Chang

Bench scale experiments were carried out with four biological nutrient removal(BNR) units, A/O, A2/O, Phostrip and P/L units, to investigate the behavior of phosphorus in the system and to compare the characteristics of phosphorus removal in four experimental BNR units. The influent COD/T-P ratio was varied from 22 to 64 by changing COD concentration while maintaining phosphorus concentration constant. In general sidestream BNR units such as Phostrip and P/L units outperformed mainstream BNR units such as A/O and A2/O units in terms of phosphorus removal. While phosphorus release and uptake in A/O and A2/O units became less significant at low influent COD/T-P, the phosphorus release in A2/O unit was further influenced by nitrate in return sludge and thus A2/O unit required even higher influent COD/T-P ratio for luxury uptake of phosphorus. The luxury uptake of phosphorus in Phostrip and P/L units were not affected by influent COD/T-P ratio and the adverse effect of nitrate in return sludge on anaerobic phosphorus release in P/L process was not significant due to the sludge blanket in P-stripper.


1983 ◽  
Vol 15 (3-4) ◽  
pp. 233-259 ◽  
Author(s):  
A R Pitman ◽  
S L V Venter ◽  
H A Nicholls

This paper describes three years operating experience with two full-scale biological nutrient removal activated sludge plants. Factors affecting biological phosphorus removal are highlighted and possible process improvements suggested.


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