Effect of oxygen on the stability and inducibility of the biodegradative capability of benzoate

2003 ◽  
Vol 48 (8) ◽  
pp. 247-254 ◽  
Author(s):  
Ö Çinar ◽  
T. Deniz ◽  
C.P.L. Grady

Anoxic zones in biological nitrogen removal systems are typically open to the atmosphere and receive oxygen from the atmosphere and the recirculation flow from the aerobic zone. This raises the question of how such oxygen input might influence the stability and inducibility of the enzyme systems involved in biodegradation of aromatic compounds. To investigate this, various amounts of oxygen were added to mixed culture denitrifying chemostats receiving benzoate at 667 mg/h as chemical oxygen demand (COD), and the stability and inducibility of the culture’s benzoate biodegradative capability (BBC) were tested in aerobic and anoxic fed-batch reactors (FBRs). Cultures from chemostats receiving oxygen at 0, 33, 133, 266, and 466 mg O2/h lost almost all of their anoxic BBC within one hour after being transferred to an aerobic FBR and the first three cultures did not recover it upon being returned to anoxic conditions. The last two cultures recovered their anoxic BBC between 9 and 16 h during the 16 h aerobic exposure period that preceded their return to anoxic conditions and continued to increase their anoxic BBC as they were retained under anoxic conditions. In contrast, the culture from a chemostat receiving oxygen at 67 mg O2/h retained its anoxic BBC longer, recovered it within 3 h after its return to anoxic conditions, and increased it linearly thereafter. None of the cultures developed any aerobic BBC during the 16 h aerobic exposure period in FBRs. The results suggest that higher oxygen inputs into anoxic reactors helped the mixed microbial cultures recover and/or induced anoxic BBC more easily when they were exposed to alternating aerobic/anoxic environments. The exceptional behavior of the culture from the chemostat receiving oxygen at a rate of 67 mg O2/h may have been caused by the presence of a protective mechanism against the toxic forms of oxygen.

Author(s):  
A.A. Dronic A.A. ◽  

The article presents an assessment of the stability of introduced cherry varieties to spring return frosts in 2020 in the conditions of the sharply continental climate of the Astrakhan region. As a result of unfavorable weather conditions, the total damage score of all varieties was 2-5 points. Almost all the studied varieties showed an insufficient level of resistance to recurrent frosts.


1986 ◽  
Vol 51 (6) ◽  
pp. 1259-1267
Author(s):  
Josef Horák ◽  
Petr Beránek

A simulation apparatus for the experimental study of the methods of control of batch reactors is devised. In this apparatus, the production of heat by an exothermic reaction is replaced by electric heating controlled by a computer in a closed loop; the reactor is cooled with an external cooler whose dynamic properties can be varied while keeping the heat exchange area constant. The effect of the cooler geometry on its dynamic properties is investigated and the effect of the cooler inertia on the stability and safety of the on-off temperature control in the unstable pseudostationary state is examined.


Author(s):  
Hussain Aqeel ◽  
Mahendran Basuvaraj ◽  
Steven N. Liss

BNR granules rich in amyloid adhesins and denitrifying bacteria were formed in the SBRs that were operated with extended anoxic conditions.


Materials ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2491
Author(s):  
Elena Garcia ◽  
Julio Torres ◽  
Nuria Rebolledo ◽  
Raul Arrabal ◽  
Javier Sanchez

The number of reinforced concrete structures subject to anoxic conditions such as offshore platforms and geological storage facilities is growing steadily. This study explored the behaviour of embedded steel reinforcement corrosion under anoxic conditions in the presence of different chloride concentrations. Corrosion rate values were obtained by three electrochemical techniques: Linear polarization resistance, electrochemical impedance spectroscopy, and chronopotenciometry. The corrosion rate ceiling observed was 0.98 µA/cm2, irrespective of the chloride content in the concrete. By means of an Evans diagram, it was possible to estimate the value of the cathodic Tafel constant (bc) to be 180 mV dec−1, and the current limit yielded an ilim value of 0.98 µA/cm2. On the other hand, the corrosion potential would lie most likely in the −900 mVAg/AgCl to −1000 mVAg/AgCl range, whilst the bounds for the most probable corrosion rate were 0.61 µA/cm2 to 0.22 µA/cm2. The experiments conducted revealed clear evidence of corrosion-induced pitting that will be assessed in subsequent research.


2006 ◽  
Vol 111 ◽  
pp. 99-102 ◽  
Author(s):  
A.A. Ahmed ◽  
Faiz Mohammad

The films of polyaniline:polyethyleneterephthalate films were prepared by polymerizing aniline soaked in polyethyleneterephthalate films of different thicknesses. The films were characterized by FTIR as well as for their electrical properties. The electrical properties of the films were observed to be of good quality as almost all the films showed a great increase in their electrical conductivity from insulator to semiconductor region after doping with hydrochloric acid. All the films in their doped state follow the Arrhenius equation for the temperature dependence of electrical conductivity from 35 to 115oC. The thermooxidative stability was studied by thermogravimetry and differential thermal analysis. The stability in terms of dc electrical conductivity retention was also studied under ambient conditions by two slightly different techniques viz. isothermal and cyclic techniques. The dc electrical conductivity of the films was found to be stable below 90oC for all the films under ambient conditions.


2013 ◽  
Vol 67 (9) ◽  
Author(s):  
Karina Michalska ◽  
Stanisław Ledakowicz

AbstractThis work studies the influence of the alkali pre-treatment of Sorghum Moench — a representative of energy crops used in biogas production. Solutions containing various concentrations of sodium hydroxide were used to achieve the highest degradation of lignocellulosic structures. The results obtained after chemical pre-treatment indicate that the use of NaOH leads to the removal of almost all lignin (over 99 % in the case of 5 mass % NaOH) from the biomass, which is a prerequisite for efficient anaerobic digestion. Several parameters, such as chemical oxygen demand, total organic carbon, total phenolic content, volatile fatty acids, and general nitrogen were determined in the hydrolysates thus obtained in order to define the most favourable conditions. The best results were obtained for the Sorghum treated with 5 mass % NaOH at 121°C for 30 min The hydrolysate thus achieved consisted of high total phenolic compounds concentration (ca. 4.7 g L−1) and chemical oxygen demand value (ca. 45 g L−1). Although single alkali hydrolysis causes total degradation of glucose, a combined chemical and enzymatic pre-treatment of Sorghum leads to the release of large amounts of this monosaccharide into the supernatant. This indicates that alkali pre-treatment does not lead to complete cellulose destruction. The high degradation of lignin structure in the first step of the pre-treatment rendered the remainder of the biomass available for enzymatic action. A comparison of the efficiency of biogas production from untreated Sorghum and Sorghum treated with the use of NaOH and enzymes shows that chemical hydrolysis improves the anaerobic digestion effectiveness and the combined pre-treatment could have great potential for methane generation.


In this paper three sustainable approaches are made in waste management option. Firstly primary treated domestic sewage is treated by aquatic macrophytes using duckweed, water hyacinth and water lettuce. Biochemical Oxygen Demand (BOD), Chemical Oxygen Demand (COD), Total Suspended Solids (TSS), Total Dissolved Solids (TDS), Phosphate, Nitrates are tested before and after. Result indicates in terms of water quality, almost all three plants shows same removal efficiencies. BOD and TSS removal efficiency is attained more than 95%. COD and TDS removal is reached upto 50% for almost all plants. Secondly the used aquatic macrophytes for wastewater treatment is again used for generation of biogas (water lettuce unit, duckweed unit, water lettuce unit). In addition to three aquatic macrophytes, sludge is collected from aquatic macrophyte unit for generation of biogas. Comparison is made with conventional cow dung biogas unit. Result indicates water lettuce and duckweed produce biogas at earlier stage itself and water hyacinth takes some time for starting of biogas production. This may be due to the structure and texture causes some time for decomposition. Sludge gives maximum biogas generation among all experimental setup. Also in this study cow dung did not give biogas more may be due to poor blend ratio of cow dung with water is one of the reason.


2019 ◽  
Vol 12 (9) ◽  
pp. 1383-1389
Author(s):  
Sakchai Ruenphet ◽  
Kornkamon Paditporn ◽  
Darsaniya Punyadarsaniya ◽  
Tippawan Jantafong ◽  
Kazuaki Takehara

Aim: This study aimed to evaluate the bactericidal and virucidal activity of food additive grade calcium hydroxide (FdCa(OH)2) under various concentrations, organic material conditions, and exposure duration including its stability. Materials and Methods: The FdCa(OH)2 powder as well as the 0.17% and 3% solutions were evaluated for bacteria and virus inactivating efficacies against Salmonella infantis (SI), Escherichia coli, Newcastle disease virus (NDV), and avian influenza virus (AIV), in the absence or presence of organic materials. In addition, the stability of FdCa(OH)2, was also examined using wet-dry conditions and under sunlight. Results: The FdCa(OH)2 powder could inactivate both NDV and AIV in the absence and presence of organic materials within a 3 min exposure period. The bactericidal efficacy using solution form revealed that 0.17% and 3% of FdCa(OH)2 could inactivate SI in the absence and presence of organic materials within 3 min of exposure. However, 3% of FdCa(OH)2 inactivated E. coli both with and without organic materials within 3min, while 0.17% required 5 min to be efficacious. The virucidal efficacy also showed that 0.17% FdCa(OH)2 could inactivate NDV in the absence and presence of organic materials within 10 min and 30 min, respectively. However, AIV inactivation was achieved within 30 sec under all conditions. In addition, under wet and dry conditions, FdCa(OH)2 powder demonstrated high efficacy when re-suspended at least 16 times for NDV and 7 times for AIV. Simultaneously, the FdCa(OH)2 powder retained its efficacy under the sunlight during up to 4 months for NDV and at least 6 months for AIV. Conclusion: The present study indicates that FdCa(OH)2 powder and solutions could inactivate SI, E. coli, NDV, and AIV while retaining good stability under challenging environmental conditions. Finally, the FdCa(OH)2 is safe for consumers because it is of food additive grade and can be useful as an alternative disinfectant, especially for biosecurity enhancement on and around poultry farms.


2018 ◽  
Vol 8 (20) ◽  
Author(s):  
Ebenezer Olasunkanmi Dada ◽  
Hadijat Oluwatobi Osilagun ◽  
Kelechi Longinus Njoku

Introduction. In spite of doubts over the safety and nutritional benefits of singed cowhide meat, called ponmo in some parts of Nigeria, and its potential negative impact on the hide and leather industry, consumption in Nigeria and many parts of Africa has continued unabated. Objectives. In the present study, physicochemical and genotoxic assessments of wastewater used to rinse ready-to-cook singed cowhide meat were carried out. Methods. Physicochemical analyses were carried out using the American Public Health Association procedures, while genotoxic assessment was carried out using Allium cepa chromosome assay. Results. The results of the physicochemical analyses indicated that some of the parameters, especially metals, were within the threshold of the limits set by the country's regulatory agencies, but some parameters like phosphate, chloride, nitrate, biological oxygen demand, and chemical oxygen demand were higher in concentration. The wastewater inhibited the growth of A. cepa roots and caused a decrease in its mitotic index relative to the control onions exposed to water only. The highest root growth inhibition of 72.14% was induced by a 10% wastewater concentration, while the lowest (53.57%) was induced by a 5% wastewater concentration. In addition, the wastewater induced bi-nucleated, attached, vagrant, C-mitosis, sticky and bridged chromosomal aberrations. Wastewater at a 5% concentration induced the highest significant (P < 0.05) percentage chromosome aberration of 36.62% at 48 hours of exposure. Sticky chromosomes had the highest significant frequency (P <0.01) at the end of the 72-hour exposure period. No chromosomal aberration was observed in the control. Conclusions. These results indicate that singed cowhide meat wastewater is potentially genotoxic and environmentally harmful. Governments, public health practitioners, and relevant stakeholders should work in synergy to discourage the habit of processing cowhide into cowhide meat. Competing Interests. The authors declare no competing financial interests.


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