Production of biopesticides using wastewater sludge as a raw material - effect of process parameters

2003 ◽  
Vol 48 (8) ◽  
pp. 239-246 ◽  
Author(s):  
M.L. Tirado Montiel ◽  
R.D. Tyagi ◽  
J.R. Valero ◽  
R.Y. Surampalli
Keyword(s):  
Shake Flask ◽  
Viable Cell ◽  
Toxin Production ◽  
Agitation Speed ◽  
Wastewater Sludge ◽  
Raw Material ◽  
Effect Of Temperature ◽  
Spore Count ◽  
Sludge Hydrolysis ◽  
Raw Wastewater

Production of Bacillus thuringiensis (Bt) based biopesticide was studied using hydrolysed or raw wastewater sludge as a raw material. The sludge hydrolysis was carried out at different pH (2, 4 and 6). The sludge after hydrolysis was sterilised at 121°C for 30 minutes. The effect of temperature, pH and agitation speed on growth and toxin production was also investigated. The pH in the shake flask was controlled by adding 0.1 M TRIS buffer. The progress of the biopesticide production process was monitored by measuring viable cell count (VC), spore count (SC) and entomotoxicity (Tx). The entomotoxicity was measured against spruce budworm. Control of pH substantially increased the entomotoxicity of the final product. Increased agitation speed resulted in higher VC, SC and Tx values.

Effect of surface active agents on the production of biopesticides using wastewater sludge as a raw material

2001 ◽  
Vol 44 (10) ◽  
pp. 253-259 ◽  
Author(s):  
A.S. Vidyarthi ◽  
R.D. Tyagi ◽  
J.R. Valéro
Keyword(s):  
Bacillus Thuringiensis ◽  
Shake Flask ◽  
Spruce Budworm ◽  
Tween 80 ◽  
Wastewater Sludge ◽  
Raw Material ◽  
Viable Cells ◽  
Surface Active Agents ◽  
Surface Active ◽  
Effect Of Surface

Effect of surface active agents on the production of Bacillus thuringiensis based biopesticides using secondary wastewater sludge as a raw material was studied. The experiments were conducted in a shake flask and nine different commercial surface active agents were used at a concentration of 0.2% (v/v). The sludge samples after addition of surface active agents were sterilised at 121°C for 30 min. The progress of biopesticide production was monitored by measuring viable cells and viable spores counts. The entomotoxicity was determined through bioassay against 3rd instar larvae of spruce budworm. ATPLUS 522, Tween 80 and Tween 85 increased the entomotoxicity potential in comparison to the control. The highest increase of 24% in entomotoxicity was observed when sludge was fortified with Tween 80.

Simultaneous production of biopesticide and alkaline proteases by Bacillus thuringiensis using sewage sludge as a raw material

2002 ◽  
Vol 46 (10) ◽  
pp. 247-254 ◽  
Author(s):  
R.D. Tyagi ◽  
V. Sikati Foko ◽  
S. Barnabe ◽  
A.S. Vidyarthi ◽  
J.R. Valéro ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Protease Activity ◽  
Synthetic Medium ◽  
Viable Cell ◽  
Wastewater Sludge ◽  
Raw Material ◽  
Alkaline Proteases ◽  
Ph Optimum ◽  
Simultaneous Production ◽  
Computer Controlled

The simultaneous production of Bacillus thuringiensis (Bt) based biopesticide and proteases was studied using synthetic medium and wastewater sludge as a raw material. The studies were conducted in shake flask and computer controlled 15-L capacity fermentors. Measuring viable cell and spore counts, entomotoxicity and protease activity monitored the progress of the biopesticide production process. A higher viable cell count and spore count was observed in synthetic Soya medium, however, higher entomotoxicity and protease activity were observed in wastewater sludge medium. Thus, the wastewater sludge is a better raw material than commercial Soya medium for the biopesticides and enzyme production. The maximum entomotoxicity and protease activity observed in the fermentor was 9,332 IU/μL and 4.58 IU/mL, respectively. The proteases produced by Bt were also characterised. Two types of proteases were detected; neutral proteases with pH optimum 7.0 and alkaline proteases with pH optimum 10-11. Further, two types of alkaline proteases were detected; one having a pH and temperature optimum at 10 and 50°C while the other at 11 and 70°C. The protease thermal stability was found to increase in the presence of CaCl2, indicating the proteases were metalloproteases.

Effect of strain and medium variation on mosquito toxin production by Bacillus thuringiensis var. israelensis

1982 ◽  
Vol 28 (9) ◽  
pp. 1089-1092 ◽  
Author(s):  
Robert A. Smith
Keyword(s):  
Bacillus Thuringiensis ◽  
Growth Medium ◽  
Shake Flask ◽  
Culture Medium ◽  
Toxin Production ◽  
Shake Flask Culture ◽  
Mosquito Larvae ◽  
Flask Culture ◽  
Strain Variation ◽  
Spore Count

The effect of strain variation and culture medium on production of toxin lethal to mosquito larvae by Bacillus thuringiensis var. israelensis serotype H-14 was investigated. Shake flask culture of B. thuringiensis H-14 strains showed varied ability to produce toxins lethal to mosquito larvae dependent upon the particular strain and growth medium used. Buffered media demonstrated no better mosquito toxicity than did unbuffered media that ranged in pH from 5.7 to 8.1 at harvest. Although toxin production is associated with sporulation, spore count was generally not proportional to toxin produced for those strains and media evaluated.

scholarly journals Effect of temperature on acid hydrolysis of Jerusalem artichoke as raw material for ethanol production

2013 ◽  
pp. 279-287 ◽  
Author(s):  
Radojka Razmovski ◽  
Vesna Vucurovic ◽  
Uros Miljic ◽  
Vladimir Puskas
Keyword(s):  
Hold Time ◽  
Jerusalem Artichoke ◽  
Raw Material ◽  
Effect Of Temperature ◽  
Industrial Biotechnology ◽  
Microbial Conversion ◽  
Experimental Conditions ◽  
Theoretical Yield ◽  
Renewable Raw Material ◽  
Layer Chromatography

Jerusalem artichoke (JA) is a low-requirement crop, which does not interfere with food chain, and is a promising carbon source for industrial fermentation. Microbial conversion of such a renewable raw material to useful products, such as ethanol, is an important objective in industrial biotechnology. In this study, ethanol was efficiently produced from the hydrolyzates of JA obtained at different pH values (pH 2.5, pH 3.0 and pH 3.5), temperature (120, 130, 132 and 134?C) and hold time (30 and 60 min) by Saccharomyces cerevisiae. The efficient degradation of JA by HCl under certain experimental conditions was confirmed by thin-layer chromatography. Ethanol concentration of 7.52% (w/w), which corresponds to 93.89 % of the theoretical yield is achieved by ethanol fermentation of JA hydrolyzate obtained at pH 2.5.

scholarly journals Effect of Temperature on Pyrolysis Oil Using High-Density Polyethylene and Polyethylene Terephthalate Sources From Mobile Pyrolysis Plant

2020 ◽  
Vol 8 ◽  
Author(s):  
Ruktai Prurapark ◽  
Kittwat Owjaraen ◽  
Bordin Saengphrom ◽  
Inpitcha Limthongtip ◽  
Nopparat Tongam
Keyword(s):  
Polyethylene Terephthalate ◽  
High Density Polyethylene ◽  
Main Product ◽  
Carbon Number ◽  
High Density ◽  
Raw Material ◽  
Effect Of Temperature ◽  
Pyrolysis Oil ◽  
Boiling Points ◽  
And Performance

This research aims to study the effect of temperature, collecting time, and condensers on properties of pyrolysis oil. The research was done be analyzing viscosity, density, proportion of pyrolysis products and performance of each condenser towers for the pyrolysis of high-density polyethylene (HDPE) and polyethylene terephthalate (PET) in the mobile pyrolysis plant. Results showed that the main product of HDPE resin was liquid, and the main product of PET resin was solid. Since the pyrolysis of PET results in mostly solid which blocked up the pipe, the analysis of pyrolysis oil would be from the use of HDPE as a raw material. The pyrolysis of HDPE resin in the amount of 100 kg at 400, 425, and 450°C produced the amount of oil 22.5, 27, and 40.5 L, respectively. The study found that 450°C was the temperature that gives the highest amount of pyrolysis oil in the experiment. The viscosity was in the range of 3.287–4.850 cSt. The density was in the range of 0.668–0.740 kg/L. The viscosity and density were increased according to three factors: high pyrolysis temperature, number of condensers and longer sampling time. From the distillation at temperatures below 65, 65–170, 170–250, and above 250°C, all refined products in each temperature range had the carbon number according to their boiling points. The distillation of pyrolysis oil in this experiment provided high amount of kerosene, followed by gasoline and diesel.

Effect of Temperature on Growth and Alpha Toxin Production by Clostridium perfringens1

1979 ◽  
Vol 42 (11) ◽  
pp. 848-851 ◽  
Author(s):  
Y. PARK ◽  
E. M. MIKOLAJCIK
Keyword(s):  
Growth Phase ◽  
Incubation Temperature ◽  
Ground Beef ◽  
Population Level ◽  
Toxin Production ◽  
Stationary Growth Phase ◽  
Effect Of Temperature ◽  
Alpha Toxin ◽  
Stationary Growth ◽  
Incubation Temperatures

Growth and alpha toxin production by a strain of Clostridium perfringens was determined in Thioglycollate medium, beef broth with ground beef, and beef broth with ground beef and soy protein. Incubation temperatures ranged from 15 to 50 C. In Thioglycollate medium, maximum alpha toxin production occurred at 35 C and was 40 times greater than that observed at 45 C. However, generation time and maximum population were approximately the same at 35 and 45 C. At 15 C, a two log cycle reduction in viable counts occurred within 6 h. Irrespective of incubation temperature, alpha toxin levels in Thioglycollate medium declined as the incubation period was extended beyond the stationary growth phase. In the beef broth with ground beef system which was studied at 35 C only, the organism grew slower and produced less toxin than in Thioglycollate medium. The amount of alpha toxin detected was influenced to a greater extent by the incubation time and temperature, the holding time beyond the stationary growth phase, and the growth medium than by the population level of C. perfringens.

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