Comparison of the quantity and quality of the microbiological content of sludge in countries with low and high content of pathogens

2002 ◽  
Vol 46 (10) ◽  
pp. 17-24 ◽  
Author(s):  
B. Jimínez ◽  
C. Maya ◽  
E. Sãnchez ◽  
A. Romero ◽  
L. Lira ◽  
...  
Keyword(s):  
Giardia Lamblia ◽  
Microbiological Quality ◽  
The United States ◽  
Salmonella Typhi ◽  
Fecal Coliforms ◽  
Residual Water ◽  
Industrialized Countries ◽  
Treatment Processes ◽  
Residual Sludge

The parasites present in the residual sludge generated by the diverse treatment processes of residual water vary, depending on the socioeconomic and epidemiological conditions of different communities. And although data exist at the global level regarding the microbiological quality of sludge, these data are practically nonexistent in developing countries. Therefore, this project compares the content of the four principal groups of parasites in residual sludge from the United States and Mexico. The marked differences between the orders of magnitude found in the two countries (coliphages 103-106 and 1.3 × 105 PFU/gTS, fecal coliforms 107-1010 and 107, Salmonella typhi 106-108 and 103 MPN/gTS, Giardia lamblia 102-104 and 102 cysts/gTS and helminth ova from 73-177 and <1 to 10 viable HO/gTS, respectively) will permit proposing real, effective and economical stabilization processes (undoubtedly different from those of industrialized countries), and will thus take advantage of the benefits represented by the reuse and disposal of sludge, in addition to developing regulations in agreement with the conditions of each region.

Incidence of Vibrio parahaemolyticus in and the Microbiological Quality of Seafood in North Carolina1

1980 ◽  
Vol 43 (10) ◽  
pp. 769-773 ◽  
Author(s):  
C. R. HACKNEY ◽  
B. RAY ◽  
M. L. SPECK
Keyword(s):  
Vibrio Parahaemolyticus ◽  
Fecal Coliform ◽  
Microbiological Quality ◽  
The United States ◽  
Statistical Correlation ◽  
Fecal Coliforms ◽  
Processing Plants ◽  
United States Food ◽  
Coagulase Positive Staphylococci

Microbiological analyses of 716 seafood samples over a 3-year period revealed that the microbiological quality of fresh seafood in North Carolina was generally acceptable. The mean aerobic counts (APC) and fecal coliform counts were low as was the occurrence of enteric pathogens, except for Vibrio parahaemolyticus and coagulase-positive staphylococci. Salmonella species were isolated from three samples, but the fecal coliform counts of these samples far exceeded the shellfish standard of the United States Food and Drug Administration. Coagulase-positive staphylococci were isolated in low numbers from nearly all the different types of seafoods; unpasteurized crabmeat and head-peeled shrimp samples showed the highest counts (10% of these samples had numbers which exceeded 100/g). V. parahaemolyticus occurred frequently in fresh seafood (overall 46% of the samples were positive) and its numbers showed a definite seasonal variation. No positive statistical correlation was found between the numbers of V. parahaemolyticus and the bacteriological indices, such as coliforms, fecal coliforms, enterococci and APC. Processing practices were found to influence the occurrence of V. parahaemolvticus in seafood; for example, improperly cleaned flumes were found to be a reservoir for V. parahaemolyticus in mechanical scallop processing plants. Also ‘picking’ waste ‘containers’ were found to be sources of V. parahaemolyticus in crab processing plants. Some processing practices such as heat shocking of oysters to facilitate opening were found to reduce the numbers of V. parahaemolyticus. Fifty V. parahaemolyticus isolates from different seafoods were tested for their Kanagawa reaction and all were found to be negative.

Microbiological Quality of Fresh Blue Crabmeat, Clams and Oysters

1983 ◽  
Vol 46 (11) ◽  
pp. 978-981 ◽  
Author(s):  
B. A. WENTZ ◽  
A. P. DURAN ◽  
A. SWARTZENTRUBER ◽  
A. H. SCHWAB ◽  
R. B. READ
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Geometric Means ◽  
Colony Forming Units ◽  
Plate Counts ◽  
The Mean ◽  
Eastern Oysters

The microbiological quality of fresh blue crabmeat, soft- and hardshell clams and shucked Eastern oysters was determined at the retail (crabmeat, oysters) and wholesale (clams) levels. Geometric means of aerobic plate counts incubated at 35°C were: blue crabmeat 140,000 colony-forming units (CFU)/g, hardshell clams, 950 CFU/g, softshell clams 680 CFU/g and shucked Eastern oysters 390,000 CFU/g. Coliform geometric means ranged from 3,6/100 g for hardshell clams to 21/g for blue crabmeat. Means for fecal coliforms or Escherichia coli ranged from <3/100 g for clams to 27/100 g for oysters, The mean Staphylococcus aureus count in blue crabmeat was 10/g.

VERMICOMPOSTING TECHNOLOGY FOR STABILIZING THE SEWAGE SLUDGE FROM RURAL WASTE WATER TREATMENT PLANTS

2008 ◽  
Vol 3 (1) ◽  
Author(s):  
V. Lina Cardoso ◽  
C. Esperanza Ramírez ◽  
E. Violeta Escalante
Keyword(s):  
Rural Communities ◽  
Sewage Sludge ◽  
Low Cost ◽  
Waste Water Treatment ◽  
Treatment Plant ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Nitrogen And Phosphorus ◽  
Sludge Management

There are problems associated with sludge management in small treatment plants (<10 L/s) located in rural communities, due to costly conventional technology for sludge stabilization. Many of these plants have only sludge drying beds. Mexican Institute of Water Technology has proposed developing suitable low-cost technologies, one of which is vermicomposting a biodegradation system using earthworms of the species Eisenia foetida (earthworm) which stabilize sludge and reduce its pathogenicity. The objective of this work is to present two case studies where vermicomposting technology has been applied in Mexico. The first study corresponds to a plant where 4.8 m3/month of sludge are produced; for these wastes, a vermicomposting system was built and installed. The second study is a treatment plant where 9 m3/month of sludge are produced; experimental tests were conducted with sludge and water hyacinth and a vermicomposting system was designed. The vermicomposts were analyzed using parameters defined by Mexican standards. In regards to stabilization, TVS was reduced by 38% and the microbiological quality of the vermicompost was Class A and B, with a reduction in fecal coliforms and Helminth eggs according to NOM-004-SEMARNAT-2002. A CRETI (Corrosivity, Reactivity, Explosivity, Toxicity and Ignitability) analysis (NOM-052-SEMARNAT-2005) was used to show that the process reduced the concentration of releasable sulfides. The agronomic quality of the vermicompost exhibited a high content of organic matter comparable to many organic manures and high content of nutrients such as nitrogen and phosphorus. It is concluded that it is possible to improve the conditions of sewage sludge management in small plants of rural communities with a minimum investment (less than $10,000.00 USD) and with a requirement of a minimum area of 60 to 70 m2 for a production of less than 9 m3/month of dehydrated sludge (80% humidity).

scholarly journals CALIDAD MICROBIOLÓGICA DE Ulva lactuca Y Ulva clathrata (CHLOROPHYTA) DE LA COSTA DE LA PAZ, B.C.S., MÉXICO

2008 ◽  
Vol 23 (1-2) ◽  
pp. 35
Author(s):  
R. N. Águila-Ramírez ◽  
C. J. Hernández-Guerrero ◽  
S. Rodríguez-Astudillo ◽  
R. Guerrero-Caballero
Keyword(s):  
Water Quality ◽  
Microbiological Quality ◽  
Marine Organisms ◽  
Ulva Lactuca ◽  
Fecal Coliforms ◽  
Human Consumption ◽  
La Paz ◽  
Ecological Criteria ◽  
Ulva Clathrata

Microbiological quality of Ulva lactuca and Ulva clathrata (Chlorophyta) along the coastline of La Paz, B.C.S., México Total, fecal coliforms and the enterococcus group in Ulva lactuca and U. clathrata were measured in four localities of the coastline of La Paz, B.C.S, Mexico. Levels of fecal coliforms in water were below the standard of the Ecological Criteria of Water Quality (EC-WQ) which indicates a healthy La Paz inlet (below 200 MPN/100ml). Values of fecal coliforms in algae were below the NOM-031-SSA1-1993 levels determined for marine organisms destined for human consumption. These values were high only in few months. No enterococci were detected in the samples.

Microbiological Quality of Frozen Cream-Type Pies Sold in Canada

1983 ◽  
Vol 46 (1) ◽  
pp. 34-40 ◽  
Author(s):  
E. C. D. TODD ◽  
G. A. JARVIS ◽  
K. F. WEISS ◽  
G. W. RIEDEL ◽  
S. CHARBONNEAU
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Microbiological Quality ◽  
The United States ◽  
National Guidelines ◽  
E Coli ◽  
Yeasts And Molds

Ten types of frozen cream-type pies, manufactured in Canada and imported from the United States, were analyzed for aerobic colony counts, yeasts and molds, coliforms, Escherichia coli, Staphylococcus aureus and Salmonella. The variations in counts depended more on the manufacturer than on the type of pie and the ingredients used. Five of the 465 examined pies had an excess of 105 aerobic colony counts/g, whereas the median value for all the pies examined was between 102 and 103 CFU/g. E. coli and S. aureus were present in few pies, mainly made by one manufacturer, but there was no correlation between high aerobic colony counts and these organisms. Salmonella was not found in any of the pies. Percentage distributions of the estimated ‘population’ of pies available nationally at the time of the survey were statistically determined. These were then compared with suggested national guidelines in the form of a three-class acceptance plan based on United States surveys and desirable manufacturing practices. These indicate that pies should contain aerobic colony counts of <50,000/g, yeast and mold counts of <500/g, S. aureus counts of <100/g, coliform counts of <50/g, E. coli counts of <10/g, and no Salmonella. Three of the six manufacturers would have had an estimated 5.4 to 32.6% of lots in excess of the guidelines at the time of the survey.

Commercialization Conditions and Practices Influence the Microbiological Quality of Mineral Waters

2008 ◽  
Vol 71 (6) ◽  
pp. 1253-1257 ◽  
Author(s):  
SÉRGIO A. P. NUNES FILHO ◽  
ANDERSON S. SANT'ANA ◽  
ADRIANO G. CRUZ
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Water Samples ◽  
Mineral Water ◽  
Microbiological Quality ◽  
Mineral Waters ◽  
Fecal Coliforms ◽  
Street Vendors ◽  
Total Coliforms ◽  
Bottled Mineral Water

The objective of the present study was to determine the microbiological quality of bottled mineral water marketed in commercial establishments and by street vendors and to evaluate the influence of the storage and maintenance conditions on the microbiological quality of the product. Ten samples from the same batches of five different brands of water were analyzed, for a total of 50 samples. Of the five brands analyzed, only one (brand A), when collected in a commercial establishment, complied with the legal Brazilian standards for mineral water with respect to the presence of total coliforms, fecal coliforms, and Pseudomonas aeruginosa. The remaining samples failed to comply with these microbiological standards for at least one of the parameters evaluated. The water samples obtained from street vendors were inferior in microbiological quality to samples from the same batch that were obtained from commercial establishments.

Minimally Processed Vegetable Salads: Microbial Quality Evaluation

2007 ◽  
Vol 70 (5) ◽  
pp. 1277-1280 ◽  
Author(s):  
HANS FRÖDER ◽  
CECÍLIA GERALDES MARTINS ◽  
KATIA LEANI OLIVEIRA de SOUZA ◽  
MARIZA LANDGRAF ◽  
BERNADETTE D. G. M. FRANCO ◽  
...  
Keyword(s):  
Fruits And Vegetables ◽  
Microbiological Quality ◽  
Positive Sample ◽  
Fecal Coliforms ◽  
Microbial Quality ◽  
Biochemical Tests ◽  
Minimally Processed ◽  
Increasing Demand ◽  
Minimally Processed Vegetables

The increasing demand for fresh fruits and vegetables and for convenience foods is causing an expansion of the market share for minimally processed vegetables. Among the more common pathogenic microorganisms that can be transmitted to humans by these products are Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella. The aim of this study was to evaluate the microbial quality of a selection of minimally processed vegetables. A total of 181 samples of minimally processed leafy salads were collected from retailers in the city of Sao Paulo, Brazil. Counts of total coliforms, fecal coliforms, Enterobacteriaceae, psychrotrophic microorganisms, and Salmonella were conducted for 133 samples. L. monocytogenes was assessed in 181 samples using the BAX System and by plating the enrichment broth onto Palcam and Oxford agars. Suspected Listeria colonies were submitted to classical biochemical tests. Populations of psychrotrophic microorganisms >106 CFU/g were found in 51% of the 133 samples, and Enterobacteriaceae populations between 105 and 106 CFU/g were found in 42% of the samples. Fecal coliform concentrations higher than 102 CFU/g (Brazilian standard) were found in 97 (73%) of the samples, and Salmonella was detected in 4 (3%) of the samples. Two of the Salmonella-positive samples had <102 CFU/g concentrations of fecal coliforms. L. monocytogenes was detected in only 1 (0.6%) of the 181 samples examined. This positive sample was simultaneously detected by both methods. The other Listeria species identified by plating were L. welshimeri (one sample of curly lettuce) and L. innocua (2 samples of watercress). The results indicate that minimally processed vegetables had poor microbiological quality, and these products could be a vehicle for pathogens such as Salmonella and L. monocytogenes.

scholarly journals Quality of raw milks in three milk processing plants in Cordoba

2014 ◽  
Vol 6 (1) ◽  
pp. 103 ◽  
Author(s):  
VIRGINIA RODRÍGUEZ, R. ◽  
ALFONSO CALDERÓN R. ◽  
OSCAR VERGARA G.
Keyword(s):  
Physicochemical Parameters ◽  
Microbiological Quality ◽  
Raw Milk ◽  
Fecal Coliforms ◽  
Processing Plant ◽  
Normal Range ◽  
Dual Purpose ◽  
Milk Processing ◽  
Processing Plants

An important condition in dairy processing plant is to obtain materials of excellent physicochemical and microbiological quality to reduce public health risks. High counts of mesophilic show contamination and lack of good manipulation in milking and milk conservation. Coliforms are a demonstration of contamination with fecal material. The norms in Colombia establish the mesophilic count as standard of microbiological quality. The purpose of this study was to determine the physicochemical and microbiological quality and health of the udders, in raw milk from dual purpose farms, supplying three milk processing plants in Cordoba (Colombia). 149 raw milk samples were collected of tree processing plants in Cordoba department. Physicochemical parameters set out in Decree 616 of 2006 were determined and aerobic mesophilic, total coliforms, fecal coliforms, and somatic cell count were established too. The variables obtained were analyzed by descriptive statistics using SAS. The physicochemical parameters were within the normal range but averages of mesophilic and somatic cells counts were high, which allows conclude that the physicochemical quality is good, but there are shortcomings in the microbiological quality and in the udder health, and is on the farms devoted to milk production where must be implemented and certified excellent management and prevention practices.

scholarly journals Microbiological evaluation of certain fruits and vegetables marketed in the city of Kenitra-Morocco

2021 ◽  
Vol 319 ◽  
pp. 01112
Author(s):  
Rahma Erahioui ◽  
Sultana Inekach ◽  
Hassna Jaber ◽  
Khadija Atfaoui ◽  
Noureddine Rhaim ◽  
...  
Keyword(s):  
Fruits And Vegetables ◽  
Microbiological Quality ◽  
Fecal Coliforms ◽  
Microbiological Analysis ◽  
Microbial Quality ◽  
Total Absence ◽  
Nutritional Qualities ◽  
Raw Foods ◽  
The City

Fruits and vegetables are essential for human health due to their nutritional qualities, but the consumption of raw foods leads to food safety problems as they are recognized as sources of transmission of infectious diseases. The objective of this work is to assess the microbiological quality of some fruits and vegetables marketed in the Kenitra city. The samples were purchased at the fruit and vegetable markets during the period from April to July 2018.The detection of microorganisms in the samples requires several steps which are weighing, dilution, isolation, enumeration and 'identification. The results of the microbiological analysis of fruits and vegetables studied show significant concentrations of the total aerobic mesophilic flora varies between 2.25 and 8.48 log10 CFU / ml, Concerning total coliforms the contamination rates vary between 1.92 and 8.42 log10UFC / ml, as well as the presence of fecal coliforms between 1.13 and 8.05 log10UFC / ml, in the same samples analyzed, strains such as: Escherichia coli, staphylococcus aureus and a total absence of salmonella and shegilla. In order to improve the safety and hygienic quality of fruits and vegetables, the application of good hygienic practices as well as continuous microbial quality control is necessary to protect the health of consumers.

scholarly journals Impact Des Facteurs De Pollution Sur La Qualite Des Eaux De La Zone Aval De La Vallee De L’oued Nekor (Al-Hoceima, Maroc)

2017 ◽  
Vol 13 (3) ◽  
pp. 43
Author(s):  
Fouad Dimane ◽  
Khadija Haboubi ◽  
Issam Hanafi ◽  
Abdelouahad El Himri ◽  
Kaouthar Andaloussi
Keyword(s):  
Microbiological Quality ◽  
Nitrate Pollution ◽  
Fecal Coliforms ◽  
Human Consumption ◽  
Well Water ◽  
Chemical Parameters ◽  
Physico Chemical ◽  
Al Hoceima ◽  
Total Bacteria

Water is a precious and essential natural resource for multiple uses. Its use for food or hygiene requires excellent physicochemical and microbiological quality. To assess the quality of well water for human consumption in the Al Hoceima region which is located in North of Morocco, a physico-chemical and bacteriological control was carried out on the basis of 10 water samples from wells in this area. The analyzes were performed on these samples by measuring the following physic-chemical parameters: temperature, pH, electric conductivity (EC), chloride (Cl -),dissolved oxygen, nitrate (NO3 -), and possibly seeking unwanted bacteria (total bacteria, total coliforms, fecal coliforms, fecal streptococci). The samples were analyzed systematically to measure all the physicochemical indicators of pollution. Correlation between pollution parameters were conducted to give a reponse element on the main causes pollution. The results performed analysis, revealed a pollution of an agricultural origin. This is confirmed by the maximum levels of chlorides, which exceed 2640 mg/l, thereby explaining the high conductivity levels (4800μs/cm). In addition 14% of analyzed wells have nitre levels 50mg/l nitrate pollution a not alarming. However, if a pollution of agricultural origin is mentioned, the lithological contribution can not be neglected on the results obtained.

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