A new analytical tool to assess health risks associated with the virological quality of drinking water (EMIRA study)

2001 ◽  
Vol 43 (12) ◽  
pp. 39-48 ◽  
Author(s):  
L. Gofti-Laroche ◽  
B. Gratacap-Cavallier ◽  
O. Genoulaz ◽  
J. C. Joret ◽  
Ph. Hartemann ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Tap Water ◽  
Enteric Virus ◽  
Analytical Tool ◽  
Raw Water ◽  
Rt Pcr ◽  
Fast Detection ◽  
Promising Tool

This work assessed the risks associated with the virological quality of tapwater using a molecular analytical tool manageable in a field survey. It combined a daily epidemiological follow-up of digestive morbidity among a panel of volunteers and a microbiological surveillance of drinking water. RT-PCR was used for detection of enterovirus, rotavirus and astrovirus. 712 cases of acute digestive conditions occurred in the 544 volunteers. 38% (9/24) raw water and 23% (10/44) tap water samples were positive for at least one virus marker with 9/10 positive tap water samples complying with bacterial criteria. No statistically significant association was found between the presence of viral markers and observed incidence of digestive morbidity. However, when an outbreak occurred, enterovirus and rotavirus RNA was detected in the corresponding stored tap water samples. Sequencing of the amplified fragments showed that the rotavirus detected was of bovine origin. This work demonstrated that enteric virus markers were common in tapwater of the study communities (characterised by a vulnerable raw water) despite absence of bacterial indicators. Tangential ultrafiltration coupled to RT-PCR allowed a simultaneous and fast detection of the study viruses from environmental samples. This process is a promising tool usable for virological water surveillance, in as much the corresponding know-how is transferred to the field professionals.

Enteric viruses in drinking water supplies

2002 ◽  
Vol 2 (3) ◽  
pp. 17-22
Author(s):  
A.P. Wyn-Jones ◽  
J. Watkins ◽  
C. Francis ◽  
M. Laverick ◽  
J. Sellwood
Keyword(s):  
Drinking Water ◽  
Heavy Rainfall ◽  
Liquid Culture ◽  
Plaque Assay ◽  
Enteric Viruses ◽  
Enteric Virus ◽  
The Other ◽  
Raw Water ◽  
Rt Pcr ◽  
Water Supplies

Two rural spring drinking water supplies were studied for their enteric virus levels. In one, serving about 30 dwellings, the water was chlorinated before distribution; in the other, which served a dairy and six dwellings the water was not treated. Samples of treated (40 l) and untreated (20 l) water were taken under normal and heavy rainfall conditions over a six weeks period and concentrated by adsorption/elution and organic flocculation. Infectious enterovirus in concentrates was detected in liquid culture and enumerated by plaque assay, both in BGM cells, and concentrates were also analysed by RT-PCR. Viruses were found in both raw water supplies. Rural supplies need to be analysed for viruses as well as bacterial and protozoan pathogens if the full microbial hazard is to be determined.

Bacterial quality of drinking water sources and antimicrobial resistance profile of Enterobacteriaceae in Bahir Dar city, Ethiopia

2014 ◽  
Vol 4 (3) ◽  
pp. 384-390 ◽  
Author(s):  
Bayeh Abera ◽  
Mulugeta Kibret ◽  
Goraw Goshu ◽  
Mulat Yimer
Keyword(s):  
Drinking Water ◽  
Antimicrobial Resistance ◽  
Water Samples ◽  
Multiple Drug Resistance ◽  
Tap Water ◽  
World Health ◽  
Most Probable Number ◽  
Bahir Dar ◽  
Probable Number

A cross-sectional study was conducted to determine the bacterial quality and antimicrobial susceptibility profiles of Enterobacteriaceae from drinking water in Bahir Dar city. A total of 140 water samples were collected in the wet and dry periods from springs (n = 4), reservoirs (n = 10) and private tap water at households (n = 126). Bacteriological analysis of water was conducted using multiple tube method. Overall, 21.4%, 18.6% and 17.8% of drinking water samples had total coliforms (TC), faecal coliforms (FC) and Escherichia coli, respectively. All spring water samples and 29.2% of private tap water had the highest TC load (18 most probable number/100 mL, 95% CI: 100). For FC, 81.4% of the drinking water supplies tested complied with both World Health Organization and Ethiopian Standards. High levels of resistance (98–100%) were observed for ampicillin by Enterobacteriaceae and Pseudomonas aeruginosa. All P. aeruginosa isolates and 20 (66.7%) of E. coli revealed multiple drug resistance. Enterobacteriaceae and P. aeruginosa isolates exhibited high levels of antimicrobial resistance. The bacterial quality of drinking water in Bahir Dar city was poor. Microbial surveillance and monitoring with periodic assessment on physical integrity of the water pipelines need to be undertaken.

scholarly journals Evaluation of Drinking Water Quality in Schools in a District Area in Hanoi, Vietnam

2020 ◽  
Vol 14 ◽  
pp. 117863022095967
Author(s):  
Dang The Hung ◽  
Vu Thi Cuc ◽  
Vu Thi Bich Phuong ◽  
Dao Thi Thanh Diu ◽  
Nguyen Thi Huyen Trang ◽  
...  
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Water Samples ◽  
Primary Schools ◽  
Tap Water ◽  
Total Iron ◽  
Drinking Water Quality ◽  
National Standard ◽  
Taste And Odor

Background: Drinking water quality affects directly human health. Assessment and prevention of water-borne diseases are crucial for primary prevention, especially for children. Objective: The main aim of this study was to investigate the quality of drinking water from tap water in preschools and primary schools in a district area in Hanoi City, Vietnam. Methods: A cross-sectional study was performed from August to October 2019. Water samples from tap water of 154 schools in a district area of Hanoi were collected to determine the quality of drinking water. From each school, at least 2 bottles of water samples were collected on the basis of a standard operating procedure (SOP). Each water sample was analyzed for microbial and physicochemical parameters, including Color, Taste and Odor, Turbidity, pH, Nitrite, Nitrate, Ammonium, Total Iron, Permanganate, Chloride, Hardness, Total Manganese, Sulfate, Arsenic, Coliform, and E.coli, by analytical methods. The obtained values of each parameter were compared with the standard values set by WHO and National Technical Regulation on Domestic Water Quality of Vietnam. Results: All of the schools employed community water system as a main source for drinking water. The results showed that all tested samples were found to be within the standards for some physicochemical properties, including Color, Taste and Odor, Hardness, Chloride, Total Iron (Fe2+ và Fe3+), Total Manganese (Mn), Nitrate (NO3–), Sulfate (SO42–), and Total Arsenic (As). On the other hand, some samples did not meet the allowable limits of the national standard, due to pH (3.9%), Turbidity (0.6%), Nitrite (3.2%), Permanganate (6.5%), and Ammonium (5.8%). Furthermore, the microbial data revealed that the substandard water samples from municipal water systems were contaminated by Coliform (9.7%) and/or E.coli (7.8%). Conclusions and recommendations: Contaminants such as bacterial and chemical agents in to drinking water could be occurred during transport, storage and handling before using by the consumer without regular surveillance. A periodic treatment procedure and monitoring system to keep the level of microbial and chemical contamination of drinking water in schools under control should be performed.

scholarly journals Drinking Water Quality of Kathmandu Valley

2015 ◽  
Vol 15 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Neel Kamal Koju ◽  
Tista Prasai ◽  
Sujen Man Shrestha ◽  
Prakash Raut
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Water Samples ◽  
Tap Water ◽  
Total Coliform ◽  
Coliform Bacteria ◽  
Kathmandu Valley ◽  
Treated Water ◽  
Who Standards

This research was conducted to analyze drinking water quality of Kathmandu valley. Total 969 water samples (392 from dug wells, 287 from deep boring, 218 from treated water, 46 from tap and 26 from other water sources) received from different places of Kathmandu, Lalitpur and Bhaktapur districts during March 2012 to March 2013. These samples were analyzed for the determination of physical (pH, temperature, conductivity, turbidity), chemical (hardness, chloride, iron, arsenic, ammonia, nitrate) and microbiological (total Coliform) parameters. It was found that the temperature and nitrate were within the WHO standards while pH, conductivity, turbidity, chloride, iron, arsenic, ammonia, and Coliform bacteria exceeded the WHO standard guideline. In ground water ( well and Boring), pH, conductivity, hardness, turbidity, iron, arsenic, chloride, ammonia and total Coliform count crossed WHO standards as 5%, 2%, 0.8%, 36%, 51%, 0.1%, 2%, 11% and 86 % respectively. Hardness was within the standard in both  reated and tap water samples. Compared to treated water, pH, arsenic and chloride were within the standard in tap water. The common problematic parameters of different sources of drinking were turbidity, iron, ammonia and conductivity. Coliform bacteria were found in 36% samples of treated water whereas 80% tap water samples were contaminated from Coliform bacteria.DOI: http://dx.doi.org/10.3126/njst.v15i1.12027Nepal Journal of Science and TechnologyVol. 15, No.1 (2014) 115-120

scholarly journals Cryptosporidium Oocyst Contamination in Drinking Water: A Case Study in Italy

2019 ◽  
Vol 16 (11) ◽  
pp. 2055 ◽  
Author(s):  
Cristina Pignata ◽  
Silvia Bonetta ◽  
Sara Bonetta ◽  
Simone M. Cacciò ◽  
Anna R. Sannella ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Tap Water ◽  
Cryptosporidium Oocyst ◽  
Treatment Plant ◽  
Drinking Water Treatment ◽  
High Variability ◽  
Rt Pcr ◽  
Cryptosporidium Spp ◽  
Pcr Targeting

The aim of this study was to evaluate the occurrence of Cryptosporidium oocysts in a drinking water treatment plant (DWTP) located in a rural area of northern Italy. Influent and effluent samples were collected at the DWTP over three years (2013–2016). In parallel, tap water samples from a public drinking fountain were collected as well. All samples were analyzed for the presence of Cryptosporidium spp. oocysts by a common method based on an immunomagnetic separation (IMS)/immunofluorescence assay (IFA), complemented by 4,6-diamidino-2-phenylindole (DAPI) staining. A reverse transcriptase-PCR (RT-PCR) protocol was added to evaluate oocyst viability. The results highlighted a high variability of oocyst concentrations across all samples (mean 4.3 ± 5.8/100 L) and a high variability in the percentage of DAPI-positive specimens (mean 48.2% ± 40.3%). Conversely, RT-PCR did not reveal the presence of viable C. parvum and C. hominis oocysts. A nested PCR targeting Cryptosporidium 18S ribosomal DNA, carried out in two water samples, confirmed the presence of a Cryptosporidium genotype associated with wild animals in the river and in tap water. The results obtained underline the vulnerability of the investigated surface water to Cryptosporidium spp. contamination. Although the recovered Cryptosporidium genotype is not a human pathogen, its presence demonstrates the existence of a potential pathogen Cryptosporidium spp. contamination risk. Moreover, these results underline the importance of also considering unconventional (not bacterial) biological contaminations (protozoa) in water resources in rural areas, including those of developed countries.

An Investigation of Public Drinking Fountains and the Changes in Bacterial Colony Counts when Tap Water is Allowed to Flow

1998 ◽  
Vol 33 (3) ◽  
pp. 363-376
Author(s):  
Veronica A. Loving ◽  
Robert W. Burden ◽  
Anne Lagrange Loving
Keyword(s):  
Drinking Water ◽  
Water Samples ◽  
Tap Water ◽  
Bacterial Colony ◽  
Public Drinking

Abstract The quality of drinking water is a worldwide concern. Public drinking fountains could contain microbes from the water as well as from the saliva, lips and fingertips of people who have used the fountain. In this study, water samples were collected and cultured for bacteria from 97 public drinking fountains at 0, 5 and 10 seconds after the water was turned on. Overall, this study found that running tap water for 5 seconds significantly decreases the numbers of microbes in the water, as does running it for 10 seconds. However, of the 27 different microbes that were isolated from 291 samples, increased colony counts were noted for many when the water was allowed to flow.

Cryptosporidium oocysts: are they still a risk for human population? A case study in Italy

2020 ◽  
Vol 30 (Supplement_5) ◽  
Author(s):  
C Pignata ◽  
S i Bonetta ◽  
S a Bonetta ◽  
S M Cacciò ◽  
A R Sannella ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
Water Samples ◽  
Tap Water ◽  
Treatment Plant ◽  
Drinking Water Treatment ◽  
Water Treatment Plant ◽  
Rt Pcr ◽  
Cryptosporidium Spp ◽  
Cryptosporidium Oocysts

Abstract Over the past decade, several outbreaks of cryptosporidiosis have been reported in many EU countries in association with the consumption of contaminated drinking water, recreational waters, food consumption and contact with animals. The aim of this study was to evaluate the occurrence of Cryptosporidium oocysts in a drinking water treatment plant (DWTP) located in a rural area of northern Italy. Influent and effluent samples at the DWTP, together with tap water samples from a public fountain were collected for three years (2013-2016). All samples were analysed for the presence of Cryptosporidium spp. oocysts by the EPA Method 1623 based on immunomagnetic separation (IMS)/immunofluorescence assay (IFA), complemented by 4,6-diamidino-2-phenylindole (DAPI) staining. The detection has been implemented with a reverse transcriptase-PCR (RT-PCR) protocol to evaluate the oocyst viability. The results highlighted a high variability of oocyst concentrations in all samples (mean 4.3 - 5.8/100 L) and a high percentage variability of the DAPI-positive specimens (mean 48.2% - 40.3%). Conversely, RT-PCR did not reveal the presence of any viable C. parvum and C. hominis oocysts. A nested PCR targeting Cryptosporidium 18S ribosomal DNA was carried out in two water samples to deepen the understanding of the obtained results. This test revealed the presence of a particular Cryptosporidium genotype associated with wild animals in the river and in tap water. Although the recovered Cryptosporidium genotype is not a human pathogen, its presence demonstrates the existence of a potential pathogen Cryptosporidium spp. contamination risk. Moreover, these results underline the importance of considering unconventional (not bacterial) biological contaminations (protozoa) in water resources in rural areas, including those of developed countries. Key messages The supply source and the drinking water treatment plant examined represent a classic example of a system that requires continuous monitoring to guarantee population health. Cryptosporidium oocysts may still be a Public Health problem even in industrialized countries, especially in rural and mountain areas.

scholarly journals Impact of Water Treatment Processes on Selected Heavy Metals Concentrations in Drinking Water Within Katsina Metropolis

2020 ◽  
pp. 336-342
Author(s):  
Yusuf Abdulrashid ◽  
Nuraddeen Abdurrahman ◽  
Kamaladdeen Abdullahi ◽  
Aisha Ado Shehu
Keyword(s):  
Heavy Metals ◽  
Drinking Water ◽  
Water Treatment ◽  
Water Samples ◽  
Tap Water ◽  
P Value ◽  
Raw Water ◽  
Value Analysis ◽  
Treatment Processes ◽  
The Impact

The research focused on the impact of water treatment processes on selected heavy metals concentrations in drinking water within Katsina metropolis. Water samples were collected from taps, sachets and Ajiwa dam raw waters and analyzed for Pb, Cu, Fe, Ni, Mn, Zn and Mg using atomic absorption spectrophotometer (AAS). The solid residues filtered from the treated samples were also analyzed using x-ray fluorescence (XRF) method. The result obtained from the water analysis shows that Cu concentration determined between (0.862-17.232) µg/L with the highest concentration below WHO limit in Ajiwa dam raw water, Pb concentration determined between (0.000-109.63) µg/L with the highest concentration above WHO limit in Ajiwa dam raw water, Ni concentration was detected in almost all the water samples, with the concentration between (0.000-0.72) µg/L, all the concentrations were below WHO limit, Mn concentration determined between (16.554-66.234) µg/L with the highest concentration within WHO limit in Ajiwa dam raw water, Zn concentration determined between (47.286-58.122) µg/L with the highest concentration within WHO limit in tap water, Mg concentration determined between (102.5-213.77) µg/L with the highest concentration within WHO limit in Ajiwa dam raw water. While the amount(%) of isolated element shows that Si(42.51), O(25.39), K(0.24), Ca(1.16), Ti(0.16), Mn(0.04), Fe(1.19), Cu(0.02), Zn(0.009), Ag(0.40), Ba(0.31), Ce(0.03), Nd(0.002), Eu(0.03), Re(0.04), Cl(1.14) and S(0.22) with Silicon with the highest percentage and Neodymium with the least percentage value. Analysis of variance (ANOVA) shows that Pb (p-value 0.000), Cu (p-value 0.000), Fe (p-value 0.000), Ni (p-value 0.490), Mn (p-value 0.007), Zn (p-value 0.000) and Mg (p-value 0.046). This results show that the concentrations of heavy metals analyzed were either removed or drastically reduced to tolerable level or standard limit set by WHO for safe drinking water through series of water treatment processes and this render the water in the areas safe for drinking for now.

scholarly journals DEVELOPMENT OF A PORTABLE HOUSEHOLD WATER DEVICE FOR IMPROVING DRINKING WATER QUALITY IN NIGERIA

2020 ◽  
Vol 5 (2) ◽  
pp. 60-66
Author(s):  
J.G. Akinbomi ◽  
I.I. Odika
Keyword(s):  
Water Quality ◽  
Drinking Water ◽  
Water Samples ◽  
Tap Water ◽  
Drinking Water Quality ◽  
State University ◽  
Water Filter ◽  
Household Water ◽  
Α Level

Pipe borne water supplied to Nigerian households may not always meet the accepted drinking water quality standards due to possible water pipe damage. The objective of this study was, therefore, to develop a portable water filter device for improving household drinking water quality. Five litres of representative tap water samples collected from Lagos State University Staff quarters were passed through the developed device. The experimental variables investigated for data acquisition were aeration period of untreated tap water; device backwashing operation cycles and retention times of tap water in the filter device. The results of the analyses of water samples for the various values of the experimental variables showed that the range values of water parameters between the treated and untreated tap water samples were 0.04-0.05, 0.02-0.03, 0.04-0.12, 0.3-0.4, 125-137, 10-20, 3-10 mg/L for barium, chromium, iron, pH, Total Dissolved Solids, hardness and chloride, respectively. Although the developed device helped in improving the quality of the tap water; the p-values for the different experimental variables were greater than α-level of 0.05 indicating the non-significance of using the developed water device. Consequently, future study will consider other experimental variables that will justify the significance of using the developed device.

ECOLOGICAL AND PHYSIOLOGICAL ASSESSMENT OF CHEMICAL COMPOSITION OF DRINKING TAP WATER IN KHANTY-MANSIYSK AND SALEKHARD

2020 ◽  
pp. 159-167
Author(s):  
V.V. Lapenko ◽  
L.N. Bikbulatova ◽  
E.M. Ternikova
Keyword(s):  
Drinking Water ◽  
Chemical Composition ◽  
Water Samples ◽  
Tap Water ◽  
Iron Concentration ◽  
Absorption Spectrometry ◽  
Northern Region ◽  
Transport Processes ◽  
Magnesium Concentration ◽  
Geochemical Environment

Water is very important for humans, as it is a solvent for metabolic products. Moreover, it is necessary for metabolism, biochemical and transport processes. The elemental status in persons depends on the geochemical environment and consumption of bioelements with food and water. The aim of the paper is to conduct a comprehensive assessment of chemical composition of drinking tap water in Khanty-Mansiysk and Salekhard. Materials and Methods. The chemical composition of 100 samples of drinking tap water was analyzed by atomic absorption spectrometry, spectrophotometry and capillary electrophoresis. All in all, there were 50 samples from Khanty-Mansiysk and 50 samples from Salekhard. The results were compared with Sanitary Rules and Norms 2.1.4.1074-01. Results. Drinking tap water in Salekhard contains a significantly higher concentration of iron, which is much above the maximum allowable concentration, if compared to water samples in Khanty-Mansiysk (p=0.03). In the cities under consideration, the water undergoes high-quality reagent-free treatment. However, the deterioration of the water supply networks in Salekhard is 3 times as high as in Khanty-Mansiysk. Calcium and magnesium concentration in water samples from Khanty-Mansiysk is 5.6 and 3.9 times lower than the MAC; in water samples from Salekhard calcium concentration is 6.3 (p=0.008) and magnesium concentration 4.6 (p<0.001) times lower than the MAC. Conclusion. The consumption of ultra-fresh drinking water leads to low intake of bioelements, which are a part of enzymes contributing to the human antioxidant defense and can result in manifestation of cardiovascular diseases. This is especially true for Salekhard with very soft drinking water and high iron concentration, which excess can exhibit prooxidant properties. Keywords: tap water, bioelements, northern region, antioxidants. Вода является важнейшим соединением для человека: необходима в качестве растворителя продуктов метаболизма и протекания обменных, биохимических и транспортных процессов. Элементный статус организма человека зависит от геохимического окружения и поступления биоэлементов с пищей и водой. Цель. Провести комплексную оценку химического состава водопроводной воды городов Ханты-Мансийск и Салехард. Материалы и методы. Методами атомно-абсорбционной спектрометрии, спектрофотометрии и капиллярного электрофореза проанализирован химический состав 100 проб водопроводной воды: по 50 из Ханты-Мансийска и Салехарда. Результаты сравнивали с СанПиН 2.1.4.1074-01. Результаты. В водопроводной воде Салехарда установлена превышающая ПДК и достоверно более высокая концентрация железа сравнительно с водой Ханты-Мансийска (р=0,03). При условии качественной безреагентной водоподготовки в изучаемых городах это обусловлено изношенностью водопроводных сетей в Салехарде, более чем в 3 раза превышающей этот показатель в Ханты-Мансийске. Концентрация кальция и магния в воде Ханты-Мансийска в 5,6 и 3,9 раза ниже ПДК; в воде Салехарда – в 6,3 (р=0,008) и 4,6 (р<0,001) раза ниже ПДК соответственно. Заключение. Употребление ультрапресной питьевой воды на фоне очень малого поступления с водой биоэлементов, входящих в состав ферментов антиоксидантной защиты организма человека, может привести к манифестации кардиоваскулярных заболеваний. Это особенно актуально для г. Салехарда с очень мягкой питьевой водой с повышенным содержанием железа, избыток которого может проявлять прооксидантные свойства. Ключевые слова: водопроводная вода, биоэлементы, северный регион, антиоксиданты.

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