Biotreatment of persistent substances using effective microorganisms

2000 ◽  
Vol 42 (12) ◽  
pp. 93-106 ◽  
Author(s):  
M. Fujita ◽  
M. Ike ◽  
Y. Kawagoshi ◽  
N. Miyata

To efficiently biotreat the persistent substances contained in wastewater, it is necessary to fully elucidate the degradation mechanisms of the substances by specific degrading microorganisms. Especially clarifying the enzymatic reactions responsible for the degradation of persistent substances is very important. Here three different kinds of aerobic or oxidative degradation reactions of persistent substances are introduced. Polyvinyl alcohol (PVA) degradation by Pseudomonas vesicularis var. povalolyticus strain PH, co-oxidative degradation of trichloroethylene (TCE) by a variety of phenol degrading bacteria, and decolorization of melanoidin by a white rot fungus Coriolus hirsutus were shown.

2008 ◽  
Vol 41 (2) ◽  
pp. 100-107 ◽  
Author(s):  
Katsuhiro Ueshima ◽  
Kazuhiro Asami ◽  
Kazuhisa Ohtaguchi

1998 ◽  
Vol 64 (5) ◽  
pp. 1766-1772 ◽  
Author(s):  
Claudia Eggert ◽  
Peter R. LaFayette ◽  
Ulrike Temp ◽  
Karl-Erik L. Eriksson ◽  
Jeffrey F. D. Dean

ABSTRACT It was recently shown that the white rot basidiomycetePycnoporus cinnabarinus secretes an unusual set of phenoloxidases when it is grown under conditions that stimulate ligninolysis (C. Eggert, U. Temp, and K.-E. L. Eriksson, Appl. Environ. Microbiol. 62:1151–1158, 1996). In this report we describe the results of a cloning and structural analysis of the laccase-encoding gene (lcc3-1) expressed by P. cinnabarinus during growth under xylidine-induced conditions. The coding region of the genomic laccase sequence, which is preceded by the eukaryotic promoter elements TATA and CAATA, spans more than 2,390 bp. The corresponding laccase cDNA was identical to the genomic sequence except for 10 introns that were 50 to 60 bp long. A sequence analysis indicated that the P. cinnabarinus lcc3-1 product has a Phe residue at a position likely to influence the reduction-oxidation potential of the enzyme’s type 1 copper center. The P. cinnabarinus lcc3-1 sequence was most similar to the sequence encoding a laccase from Coriolus hirsutus (level of similarity, 84%).


1999 ◽  
Vol 65 (4) ◽  
pp. 1798-1800 ◽  
Author(s):  
Daniel M. Larking ◽  
Russell J. Crawford ◽  
Gregor B. Y. Christie ◽  
Greg T. Lonergan

ABSTRACT Degradation of polyvinyl alcohol (PVA) was investigated by using a combination of chemical treatment with Fenton’s reagent and biological degradation with the white rot fungus Pycnoporus cinnabarinus. Inclusion of the chemical pretreatment resulted in greater degradation of PVA than the degradation observed when biological degradation alone was used.


1997 ◽  
Vol 33 (1) ◽  
pp. 35-45 ◽  
Author(s):  
NAOYUKI MIYATA ◽  
MASAKO YAMASHITA ◽  
MICHIHIKO IKE ◽  
KEISUKE IWAHORI ◽  
MASANORI FUJITA

2012 ◽  
Vol 3 (1) ◽  
pp. 20-21
Author(s):  
A.Sangeetha A.Sangeetha ◽  
◽  
K.Thanigai K.Thanigai ◽  
Narasimhamurthy Narasimhamurthy ◽  
S.K.Nath S.K.Nath

2020 ◽  
Vol 93 (9) ◽  
pp. 289-292
Author(s):  
Yumi SHIMIZU ◽  
Shuma SATHO ◽  
Taro NAKAJIMA ◽  
Hiroaki KOUZAI ◽  
Kiminori SHIMIZU

2018 ◽  
Vol 69 (1) ◽  
pp. 38-44
Author(s):  
Nicoleta Mirela Marin ◽  
Olga Tiron ◽  
Luoana Florentina Pascu ◽  
Mihaela Costache ◽  
Mihai Nita Lazar ◽  
...  

This study investigates the synergistic effects of ion exchange and biodegradation methods to remove the Acid Blue 193 also called Gryfalan Navy Blue RL (GNB) dye from wastewater. Ion exchange studies were performed using a strongly basic anion exchange resin Amberlite IRA 400. The equilibrium was characterized by a kinetic and thermodynamic points of view, establishing that the sorption of the GNB dye was subject to the Freundlich isotherm model with R2 = 0.8710. Experimental results showed that the activated resin can removed up to 93.4% when the concentration of dye solution is 5.62�10-2 mM. The biodegradation of the GNB was induced by laccase, an enzyme isolated from white-rot fungus. It was also analyzed the role of pH and dye concentration on GNB biodegradation, so 5�10-2 mM dye had a maximum discoloration efficiency of 82.9% at pH of 4. The laccase showed a very fast and robust activity reaching in a few minutes a Km value of 2.2�10-1mM. In addition, increasing the GNB concentration up to 8�10-1 mM did not triggered a substrat inhibition effect on the laccase activity. Overall, in this study we proposed a mixt physicochemical and biological approach to enhance the GNB removal and biodegradability from the wastewaters and subsequently the environment.


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