scholarly journals Assessment of the bioremediation efficacy of the mercury resistant bacterium isolated from the Mithi River

2018 ◽  
Vol 19 (1) ◽  
pp. 191-199 ◽  
Author(s):  
Bhupendra Pushkar ◽  
Pooja Sevak ◽  
Suvarna Sounderajan
Keyword(s):  
16S Rdna ◽  
River Water ◽  
Water Samples ◽  
Bacterial Isolate ◽  
Heterotrophic Bacteria ◽  
Mercury Contamination ◽  
Resistant Bacteria ◽  
Biochemical Tests ◽  
River Water Samples ◽  
Wide Range

Abstract The contamination of natural resources with mercury has gained attention due to its high toxicity to all life forms. Bioremediation of mercury using bacteria is a useful technique to remediate mercury contamination. In the present study mercury resistant bacteria (MRB) were isolated from Mithi River water samples. Total heterotrophic bacteria (THB) and MRB present in the Mithi river water samples were enumerated. The count for THB was found to be 3.7 × 106 CFU/ml. MRB enumerated in the nutrient agar medium with mercury concentrations of 50, 100 and 150 ppm had counts of 2.8 × 106, 9.1 × 105 and 5.8 × 104 CFU/ml, respectively. The minimum inhibitory concentration (MIC) of the isolated bacterium was found to be around 500 ppm of mercury, and it was selected for further analysis. The bacterial isolate was found to tolerate a wide range of salt concentrations from 5 to 35 ppt of NaCl. The bacterial isolate was characterized by using standard biochemical tests and identified by using the 16S rDNA technique. Homology analysis of the 16S rDNA gene has confirmed the identity of the bacterium as Bacillus thuringiensis strain RGN1.2 with NCBI accession no. KX832953.1. It could remove 96.72%, 90.67% and 90.10% of mercury in 48 hours at 10, 25 and 50 ppm of mercury.

On-site determination of bisphenol A in river water by a novel solid-state electrochemiluminescence quenching sensor

2017 ◽  
Vol 14 (2) ◽  
pp. 115 ◽  
Author(s):  
Xiaoying Wang ◽  
Yijie Wang ◽  
Meng Jiang ◽  
Yanqun Shan ◽  
Xiaobing Wang
Keyword(s):  
Solid State ◽  
Bisphenol A ◽  
River Water ◽  
Water Samples ◽  
High Sensitivity ◽  
Intensity Change ◽  
Electrospinning Technique ◽  
River Water Samples ◽  
Wide Range

Environmental contextBisphenol A is an endocrine disruptor, which may migrate and transfer to the environment where it presents a potential risk to the health of humans and animals. Herein, we demonstrate that electrospun nanofibers could be used to develop a highly efficient solid-state quenching sensor for on-site determination of bisphenol A in river water samples. The strategy has great potential for routine environmental analyses. AbstractA novel solid-state electrochemiluminescence (ECL) quenching sensor based on luminescent composite nanofibres for detection of bisphenol A (BPA) has been designed. Luminescent composite nanofibres of ruthenium(ii) tris(bipyridine) (Ru(bpy)32+)-doped core–shell Cu@Au alloy nanoparticles (Ru/Cu@Au) mixed with nylon 6 (PA6)–amino-functionalised multi-walled carbon nanotubes (MWCNTs), Ru/Cu@Au-MWCNTs-PA6, were successfully fabricated by a one-step electrospinning technique. The Ru/Cu@Au-MWCNTs-PA6 nanofibres, with a unique 3D nanostructure, large specific surface area and double Ru(bpy)32+-ECL signal amplification, exhibited excellent ECL photoelectric behaviours on a glassy carbon electrode. As a solid-state ECL sensor, the Ru/Cu@Au-MWCNTs-PA6 nanofibres can sensitively detect low concentrations of BPA by monitoring the BPA-dependent ECL intensity change. The detection limit for BPA is 10 pM, which is comparable or better than that in the reported assays. The sensor was successfully applied to on-site determination of BPA in river water samples obtained from eight different sampling sites with good recovery, ranging from 97.8 to 103.4%. The solid-state ECL sensor displayed wide-range linearity, high sensitivity and good stability, and has great potential in the field of environmental analyses.

Ekplorasi dan Karakterisasi Biokimia Bakteri Resisten Timbal (Pb) dari Sungai Tallo Makassar

2020 ◽  
Vol 5 (3) ◽  
Author(s):  
Fahruddin Fahruddin ◽  
Nur Haedar ◽  
Slamet Santosa ◽  
Sri Wahyuni
Keyword(s):  
Heavy Metal ◽  
Water Samples ◽  
Bacterial Isolate ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Sediment Samples ◽  
Agar Media ◽  
Biological Methods ◽  
Selection Of

Industrial progress and waste of urban domestic activities have an impact on the Pb heavy metal pollution on the environment, this will have an impact on human health. Pb metal can be overcome with biological methods by utilizing bacteria in reducing Pb metal. The study aimed to obtain the species  of resistant bacterial isolates of Pb metal and determine its characteristics. Sediment and water samples were obtained from the Tallo River. Isolation and selection of Pb metal resistant bacteria were carried out on nutrient agar media is 10 ppm added of PbAgNO3, afterwards the colonies that grew and differed were characterized by morphological morphology and several biochemical tests. The characterization results obtained 8 isolates of Pb metal resistant bacteria consisted of 8 isolates from sediment samples and 3 isolates from water samples. The characteristics of each bacterial isolate on cell morphology are the same and biochemical tests show different results.

Fabrication of dendritic nanoporous gold via a two-step amperometric approach: Application for electrochemical detection of methyl parathion in river water samples

Talanta ◽  
2021 ◽  
Vol 226 ◽  
pp. 122130
Author(s):  
Gilberto J. Silva Junior ◽  
Jéssica Soares Guimarães Selva ◽  
Anandhakumar Sukeri ◽  
Josué M. Gonçalves ◽  
Matias Regiart ◽  
...  
Keyword(s):  
River Water ◽  
Electrochemical Detection ◽  
Water Samples ◽  
Methyl Parathion ◽  
Nanoporous Gold ◽  
River Water Samples

Modified paramagnetic beads in a microfluidic system for the determination of ethinylestradiol (EE2) in river water samples

2010 ◽  
Vol 25 (6) ◽  
pp. 1376-1381 ◽  
Author(s):  
Noelia A. Martínez ◽  
Rudolf J. Schneider ◽  
Germán A. Messina ◽  
Julio Raba
Keyword(s):  
River Water ◽  
Water Samples ◽  
Microfluidic System ◽  
River Water Samples ◽  
Paramagnetic Beads

Spectrophotometry determination of Congo red in river water samples using nanosilver

2012 ◽  
Vol 94 (10) ◽  
pp. 1886-1892 ◽  
Author(s):  
R. Sahraei ◽  
A. Farmany ◽  
S.S. Mortazavi ◽  
H. Noorizadeh
Keyword(s):  
River Water ◽  
Water Samples ◽  
Congo Red ◽  
River Water Samples
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