The development of species-specific probes for a quantitative estimation of non-cultured bacteria in groundwater samples

2012 ◽  
Vol 12 (2) ◽  
pp. 212-219
Author(s):  
Annemieke Ultee ◽  
Ines Hahn ◽  
Stephanie Schwarz ◽  
Helmut König
Keyword(s):  
Drinking Water ◽  
Quantitative Estimation ◽  
Bacterial Species ◽  
Treatment Plant ◽  
Drinking Water Treatment ◽  
Water Treatment Plant ◽  
Water Production ◽  
Groundwater Samples ◽  
Production Wells ◽  
Species Specific

The aim of this study was to quantify different bacteria in drinking water production wells that supply the city of Mainz (capital of Rhineland-Palatinate, Germany). Knowledge of the counts of individual microorganisms is important for the appraisal of the hygienic and sensory quality of drinking water. Specific 16S rRNA probes were designed for 14 previously identified but non-cultured bacterial species (Ferribacterium limneticum, Hydrogenophaga sp., Methylotenera sp., Janthinobacterium sp., Pseudomonas sp., Rhizobium selenireducens, Rhodoferax sp., Nitrosomonadaceae bacterium, Thauera sp. and five strains which could not be identified to species level). With fluorescence in situ hybridization the titer of these species was determined. Total bacterial counts were 4.3 × 104 cells/mL (April) and 4.0 × 104cells/mL (December). The highest relative counts were observed for Methylotenera sp. (4.6–21.7%), F. limneticum (6.1–10.2%), Janthinobacterium sp. (6.1–7.1%), strain K-S10-Sep02 (5.8–5.9%) and Nitrosomonadaceae bacterium (4.8–5.9%). These organisms accounted for 50% (April) and 29% (December) of the total counts. Finally, single water production wells that supply drinking water were studied for the counts of Methylotenera sp., F. limneticum and Janthinobacterium sp. These counts differed between the wells and did not reflect counts in the drinking water treatment plant.

scholarly journals Metagenomic profiling of ammonia- and methane-oxidizing microorganisms in a Dutch drinking water treatment plant

2020 ◽  
Author(s):  
Lianna Poghosyan ◽  
Hanna Koch ◽  
Jeroen Frank ◽  
Maartje A.H.J. van Kessel ◽  
Geert Cremers ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
Microbial Communities ◽  
Treatment Plant ◽  
Drinking Water Treatment ◽  
Water Treatment Plant ◽  
Water Production ◽  
Sand Filter ◽  
Clade B ◽  
Drinking Water Production

AbstractElevated concentrations of ammonium and methane in groundwater can cause severe problems during drinking water production. To avoid their accumulation, raw water in the Netherlands, and many other countries, is purified by sand filtration. These drinking water filtration systems select for microbial communities that mediate the biodegradation of organic and inorganic compounds. In this study, the active layers and wall biofilm of a Dutch drinking water treatment plant (DWTP) were sampled at different locations along the filtration units of the plant over three years. We used high-throughput sequencing in combination with differential coverage and sequence composition-based binning to recover 56 near-complete metagenome-assembled genomes (MAGs) with an estimated completion of ≥70% and with ≤10% redundancy. These MAGs were used to characterize the microbial communities involved in the conversion of ammonia and methane. The methanotrophic microbial communities colonizing the wall biofilm (WB) and the granular material of the primary rapid sand filter (P-RSF) were dominated by members of the Methylococcaceae and Methylophilaceae. The abundance of these bacteria drastically decreased in the secondary rapid sand filter (S-RSF) samples. In all samples, complete ammonia-oxidizing (comammox) Nitrospira were the most abundant nitrifying guild. Clade A comammox Nitrospira dominated the P-RSF, while clade B was most abundant in WB and S-RSF, where ammonium concentrations were much lower. In conclusion, the knowledge obtained in this study contributes to understanding the role of microorganisms in the removal of carbon and nitrogen compounds during drinking water production. We furthermore found that drinking water treatment plants represent valuable model systems to study microbial community function and interaction.HighlightsMicrobial distribution was mainly influenced by sampling location within the DWTPClade A comammox Nitrospira were the dominant nitrifiers in the primary sand filterClade B was most abundant in samples from wall biofilm and the secondary filterA novel Methylophilaceae-affiliated methanotroph dominated the primary sand filter

Barcelona's water supply improvement: the brine collector of the Llobregat river

1994 ◽  
Vol 30 (10) ◽  
pp. 221-227 ◽  
Author(s):  
Jordi Martín-Alonso
Keyword(s):  
Drinking Water ◽  
Surface Water ◽  
Treatment Plant ◽  
Water Treatment Plant ◽  
Salt Mine ◽  
Water Production ◽  
Public Work ◽  
Drinking Water Production ◽  
Llobregat River

The Llobregat is a 156 km long river, which supplies 35% of the Barcelona's drinking water needs from the Sant Joan Despí Water Treatment Plant. Since the establishment of the Salt Mine Works in the Llobregat basin in 1923, a progressive salinization of the water sources has been recorded. The operation of the Brine Collector, as a public work carried out by Aigües de Barcelona (AGBAR), started in 1989; it enabled a very significant improvement in the quality of the surface water used for drinking-water production.

Experiences with the practical implementation and operation of biological nitrification for a small-scale drinking water treatment plant

2016 ◽  
Vol 16 (4) ◽  
pp. 922-930 ◽  
Author(s):  
L. Richard ◽  
E. Mayr ◽  
M. Zunabovic ◽  
R. Allabashi ◽  
R. Perfler
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
Water Supply ◽  
Treatment Plant ◽  
Drinking Water Treatment ◽  
Water Treatment Plant ◽  
Drinking Water Supply ◽  
Small Scale ◽  
Treatment Performance ◽  
Biological Nitrification

The implementation and evaluation of biological nitrification as a possible treatment option for the small-scale drinking water supply of a rural Upper Austrian community was investigated. The drinking water supply of this community (average system input volume: 20 m3/d) is based on the use of deep anaerobic groundwater with a high ammonium content of geogenic origin (up to 5 mg/l) which must be treated to prevent the formation of nitrites in the drinking water supply system. This paper describes the implementation and operation of biological nitrification despite several constraints including space availability, location and financial and manpower resources. A pilot drinking water treatment plant, including biological nitrification implemented in sand filters, was designed and constructed for a maximum treatment capacity of 1.2 m3/h. Online monitoring of selected physicochemical parameters has provided continuous treatment performance data. Treatment performance of the plant was evaluated under standard operation as well as in the case of selected malfunction events.

scholarly journals Adsorption of Carbon Dioxide by Reusing Drinking Water Treatment Plant Sludge

2017 ◽  
Vol 29 (12) ◽  
pp. 2665-2670
Author(s):  
Soleha Mohamat Yusuff ◽  
K.K. Ong ◽  
W.M.Z. Wan Yunus ◽  
A. Fitrianto ◽  
M. Ahmad ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Drinking Water ◽  
Water Treatment ◽  
Treatment Plant ◽  
Drinking Water Treatment ◽  
Water Treatment Plant ◽  
Drinking Water Treatment Plant ◽  
Water Treatment Plant Sludge

scholarly journals Bacillus purgationiresistans sp. nov., isolated from a drinking-water treatment plant

2012 ◽  
Vol 62 (1) ◽  
pp. 71-77 ◽  
Author(s):  
Ivone Vaz-Moreira ◽  
Vânia Figueira ◽  
Ana R. Lopes ◽  
Alexandre Lobo-da-Cunha ◽  
Cathrin Spröer ◽  
...  
Keyword(s):  
Drinking Water ◽  
Water Treatment ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Treatment Plant ◽  
Drinking Water Treatment ◽  
Water Treatment Plant ◽  
Rrna Gene ◽  
Rrna Gene Sequence

A Gram-positive, aerobic, non-motile, endospore-forming rod, designated DS22T, was isolated from a drinking-water treatment plant. Cells were catalase- and oxidase-positive. Growth occurred at 15–37 °C, at pH 7–10 and with <8 % (w/v) NaCl (optimum growth: 30 °C, pH 7–8 and 1–3 % NaCl). The major respiratory quinone was menaquinone 7, the G+C content of the genomic DNA was 36.5 mol% and the cell wall contained meso-diaminopimelic acid. On the basis of 16S rRNA gene sequence analysis, strain DS22T was a member of the genus Bacillus. Its closest phylogenetic neighbours were Bacillus horneckiae NRRL B-59162T (98.5 % 16S rRNA gene sequence similarity), Bacillus oceanisediminis H2T (97.9 %), Bacillus infantis SMC 4352-1T (97.4 %), Bacillus firmus IAM 12464T (96.8 %) and Bacillus muralis LMG 20238T (96.8 %). DNA–DNA hybridization, and biochemical and physiological characterization allowed the differentiation of strain DS22T from its closest phylogenetic neighbours. The data supports the proposal of a novel species, Bacillus purgationiresistans sp. nov.; the type strain is DS22T ( = DSM 23494T = NRRL B-59432T = LMG 25783T).

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