Treated wastewater will play important role in the implementation of Kuwait National Agriculture Development Plan. As a substitute for brackish or fresh water in irrigation, treated municipal wastewater can be a practical solution. Nevertheless, special health and environmental considerations should be carefully assessed when using this water source. These considerations include potential infection with human pathogens, effects of heavy metals on the public health, and the ecological consequence on soil and water resources at large. These topics were investigated under controlled environment using a range of crops and two types of irrigation water: a tertiary treated wastewater source and fresh tap water as a control. Soil, water, and plant samples were collected at fixed intervals and were analyzed for total viable count; total Coliforms; faecal Coliforms; and faecal Streptococc, Salmonellae, Shigella, Ascaris imbricoides and pathogenic viruses. In addition, we measured for nine heavy metals (Cd, Zn, Fe, Mn, Cu, Ni, Co, Cr, and Pb). Soil salinity and build-up of sodicity in soil and ground water sources also were investigated for any changes that could indicate a potential long-term degradation effect. The results after 3 years of study indicate that the generated tertiary treated wastewater is of high quality. Its potential pathogenic content is below the guidelines recommended by WHO for using wastewater in mixed agriculture, and therefore, it had no accumulative effect on the irrigated soil or the different parts of plant tissues that were analyzed. However, treated effluent have occasionally exhibited pathogenic index higher than permitted levels reflecting the fluctuating nature of wastewater treatment. Heavy metals content of irrigation water and from the irrigated soils were negligible, and the plant tissues that were analyzed contained metallic contents below the established range in IPE. The treated water had low TDS but high sodium content with 25–35 SAR values; however, the effect on the irrigated soil was insignificant due to the calcareous nature of the soil used. This study used drip irrigation system and under similar conditions no health problems will be expected when using tertiary treated wastewater for irrigation. To minimize potential risk to the consumers, specific guidelines are recommended on the type of crops to grow and the horticulture practices to be used.