scholarly journals Analysis of membrane fouling by proteins during nanofiltration of chitin alkali wastewater

2014 ◽  
Vol 4 (4) ◽  
pp. 253-262 ◽  
Author(s):  
Juan Li ◽  
Liming Zhao ◽  
Yaosong Wang ◽  
Chaoqin Chen ◽  
Jiachun Zhou ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Membrane Fouling ◽  
Protein Concentration ◽  
Large Scale ◽  
Permeate Flux ◽  
Total Proteins ◽  
Fouling Mechanism ◽  
Protein Fouling ◽  
Processing Plants ◽  
And Diffusion

The membrane fouling mechanism used during the nanofiltration (NF) of chitin alkali effluent was investigated. Tests were carried out in three large-scale chitin-processing plants with three kinds of wastewater. An alkali resistant NF membrane with molecular weight cut-off of 250 Da was employed. The reflection coefficient (σ) and diffusion coefficient (Ps) of total proteins were deduced, assuming that the proteins were single entities in the feed. Viscosity and osmosis pressure were measured to evaluate their influences on the permeate flux. Furthermore, the fraction of the protein fouling was extracted and qualitatively analyzed by mass spectrometry. Results showed that the NF permeate flux of alkali wastewater with the highest protein concentration (4.00%) was the lowest, and that σ and penetration Ps decreased with protein content growth. Over 60% of the peptides in the permeate were hydrophobic, whereas 70% of the peptides in the adsorption cake were hydrophilic. Irreversible resistance was the predominant resistance during NF processing, and the fouling behaviour of hydrophilic fractions was dominant.

scholarly journals DETERMINATION OF FOULING MECHANISM IN ULTRAFILTRATION OF ELECTROPLATING WASTEWATER

2018 ◽  
Vol 80 (3-2) ◽  
Author(s):  
Danu Ariono ◽  
Anita Kusuma Wardani ◽  
Putu Teta Prihartini Aryanti ◽  
Ahmad Nurul Hakim ◽  
I Gede Wenten
Keyword(s):  
Flow Velocity ◽  
Membrane Fouling ◽  
Membrane Surface ◽  
Cross Flow ◽  
Permeate Flux ◽  
High Concentration ◽  
Filtration Time ◽  
Electroplating Wastewater ◽  
Fouling Mechanism ◽  
Cross Flow Velocity

Wastewater from electroplating industries is usually contaminated with high concentration of hazardous materials, such as nickel, copper, and chromium. Therefore, the electroplating wastewater is one of the environmental problems that require a novel solution to reduce risks for human and environment. Ultrafiltration is a promising technology to overcome this problem due to its ability to reject all suspended solids. However, membrane fouling still becomes a major obstacle in ultrafiltration processes. Fouling reduces the permeate flux and increases membrane operational costs due to membrane cleaning. In this work, fouling mechanism that occurred in polyacrylonitrile based ultrafiltration for electroplating wastewater treatment was investigated. The effects of trans-membrane pressure (TMP) and cross flow velocity on fouling mechanism were also studied. The results showed that in the first 20 minutes, intermediate blocking was occurred on the membrane surface, while cake formation was happened for the rest of filtration time. These results were applied for all TMP and cross flow velocity.

scholarly journals Large Scale Profiling of Protein Isoforms Using Label-Free Quantitative Proteomics Revealed the Regulation of Nonsense-Mediated Decay in Moso Bamboo (Phyllostachys edulis)

Cells ◽  
2019 ◽  
Vol 8 (7) ◽  
pp. 744 ◽  
Author(s):  
Xiaolan Yu ◽  
Yongsheng Wang ◽  
Markus V. Kohnen ◽  
Mingxin Piao ◽  
Min Tu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Proteomics ◽  
Large Scale ◽  
Gene Annotation ◽  
Function Analysis ◽  
Moso Bamboo ◽  
Protein Isoforms ◽  
Label Free ◽  
Total Proteins ◽  
Protein Coding

Moso bamboo is an important forest species with a variety of ecological, economic, and cultural values. However, the gene annotation information of moso bamboo is only based on the transcriptome sequencing, lacking the evidence of proteome. The lignification and fiber in moso bamboo leads to a difficulty in the extraction of protein using conventional methods, which seriously hinders research on the proteomics of moso bamboo. The purpose of this study is to establish efficient methods for extracting the total proteins from moso bamboo for following mass spectrometry-based quantitative proteome identification. Here, we have successfully established a set of efficient methods for extracting total proteins of moso bamboo followed by mass spectrometry-based label-free quantitative proteome identification, which further improved the protein annotation of moso bamboo genes. In this study, 10,376 predicted coding genes were confirmed by quantitative proteomics, accounting for 35.8% of all annotated protein-coding genes. Proteome analysis also revealed the protein-coding potential of 1015 predicted long noncoding RNA (lncRNA), accounting for 51.03% of annotated lncRNAs. Thus, mass spectrometry-based proteomics provides a reliable method for gene annotation. Especially, quantitative proteomics revealed the translation patterns of proteins in moso bamboo. In addition, the 3284 transcript isoforms from 2663 genes identified by Pacific BioSciences (PacBio) single-molecule real-time long-read isoform sequencing (Iso-Seq) was confirmed on the protein level by mass spectrometry. Furthermore, domain analysis of mass spectrometry-identified proteins encoded in the same genomic locus revealed variations in domain composition pointing towards a functional diversification of protein isoform. Finally, we found that part transcripts targeted by nonsense-mediated mRNA decay (NMD) could also be translated into proteins. In summary, proteomic analysis in this study improves the proteomics-assisted genome annotation of moso bamboo and is valuable to the large-scale research of functional genomics in moso bamboo. In summary, this study provided a theoretical basis and technical support for directional gene function analysis at the proteomics level in moso bamboo.

scholarly journals Filtration analysis and fouling mechanisms of PVDF membrane for POME treatment

2020 ◽  
Vol 10 (3) ◽  
pp. 187-199
Author(s):  
Mohd Azwan Ahmad ◽  
Bidattul Syirat Zainal ◽  
Nashrah Hani Jamadon ◽  
Thomas Choong Shean Yaw ◽  
Luqman Chuah Abdullah
Keyword(s):  
Membrane Fouling ◽  
Membrane Surface ◽  
Water Flux ◽  
Permeate Flux ◽  
Cake Filtration ◽  
Pvdf Membrane ◽  
Fouling Mechanism ◽  
Promising Treatment ◽  
Palm Oil Mill ◽  
Treatment Design

Abstract Palm oil mill effluent (POME) is a hazardous wastewater which contains high organic constituents and salt concentrations. The ultrafiltration (UF) process is a promising treatment design used for secondary treatment such as POME. However, membrane fouling is the major problem which limits the performance of the UF. This paper describes a detailed investigation of polyvinylidiene fluoride (PVDF) membrane for the treatment of POME. The fouling behavior was analyzed by water flux, fouling mechanism, scanning electron microscopy (SEM), particle size distribution (PSD) and Energy Dispersive X-ray (EDX). It was found that a significant reduction in the permeate flux was caused by the build up of a fouling layer. Study on the fouling mechanism shows that cake filtration dominated the fouling activities on the membrane surface, compared to standard blocking, intermediate blocking, and complete blocking. This result is supported by membrane autopsy through SEM, PSD and EDX.

Algorithm of combining chromatography mass spectrometry-untargeted profiling and multivariate analysis for identification of marker-substances in samples of complex composition

2020 ◽  
Vol 86 (7) ◽  
pp. 12-19
Author(s):  
I. V. Plyushchenko ◽  
D. G. Shakhmatov ◽  
I. A. Rodin
Keyword(s):  
Mass Spectrometry ◽  
Multivariate Analysis ◽  
Large Scale ◽  
Complex Composition ◽  
Unified Protocol ◽  
Chromatography Mass Spectrometry ◽  
Marker Substances ◽  
Selection Testing ◽  
Untargeted Profiling

A viral development of statistical data processing, computing capabilities, chromatography-mass spectrometry, and omics technologies (technologies based on the achievements of genomics, transcriptomics, proteomics, metabolomics) in recent decades has not led to formation of a unified protocol for untargeted profiling. Systematic errors reduce the reproducibility and reliability of the obtained results, and at the same time hinder consolidation and analysis of data gained in large-scale multi-day experiments. We propose an algorithm for conducting omics profiling to identify potential markers in the samples of complex composition and present the case study of urine samples obtained from different clinical groups of patients. Profiling was carried out by the method of liquid chromatography mass spectrometry. The markers were selected using methods of multivariate analysis including machine learning and feature selection. Testing of the approach was performed using an independent dataset by clustering and projection on principal components.

Applied Research in Membrane Fouling in the Membrane Bioreactor Systems with Large-scale Equipment

2019 ◽  
Vol 1 (1) ◽  
pp. 1-10
Author(s):  
Kong Linghan ◽  
Zhao Weidian ◽  
Ran Deqin ◽  
Hui Bing ◽  
Lu Linguo ◽  
...  
Keyword(s):  
Membrane Fouling ◽  
Large Scale ◽  
Membrane Bioreactor ◽  
Applied Research

Predictability of fouling-potential of raw water for ultrafiltration membranes

2011 ◽  
Vol 11 (4) ◽  
pp. 481-489
Author(s):  
S. Krause ◽  
A. Obermayer
Keyword(s):  
Water Quality ◽  
Membrane Fouling ◽  
Large Scale ◽  
High Capacity ◽  
Raw Water ◽  
Ultrafiltration Membranes ◽  
Small Water ◽  
Lab Experiments ◽  
Flux Decline ◽  
Public Drinking Water

The public drinking water supply of southern Germany is characterized by a rather decentralized network. Due to the hydrogeological setting in these parts of Germany many of the small water works with an average capacity of 50 m3/h have to treat raw water extracted from karstic or cliffy aquifers. These raw waters tend to be contaminated with particles and pathogens acquired during snowmelt or after strong rainfalls. In the last decade ultrafiltration has become the technology of choice for the removal of the aforementioned contaminants. Flux decline caused by unanticipated membrane fouling is the main limitation for the application of ultrafiltration membranes. This paper describes how membrane fouling phenomena can be predicted by using a statistical approach based on data from large scale filtration systems in combination with field and lab experiments on raw water quality and membrane performance. The data defines water quality and respective fouling phenomena both in technical scale filtration plants and in lab experiments of eleven different raw waters. The method described here is more economically feasible for small water works when compared to typical pilot experiments that are used for high capacity water works.

Accidental Sustainability

2017 ◽  
Author(s):  
Geoffrey Jones
Keyword(s):  
Large Scale ◽  
Negative Impact ◽  
Positive Impact ◽  
Organic Food ◽  
The United States ◽  
Air Travel ◽  
Business Leaders ◽  
Natural Beauty ◽  
And Diffusion ◽  
Whole Foods

This chapter examines the scaling and diffusion of green entrepreneurship between 1980 and the present. It explores how entrepreneurs and business leaders promoted the idea that business and sustainability were compatible. It then examines the rapid growth of organic foods, natural beauty, ecological architecture, and eco-tourism. Green firms sometimes grew to a large scale, such as the retailer Whole Foods Market in the United States. The chapter explores how greater mainstreaming of these businesses resulted in a new set of challenges arising from scaling. Organic food was now transported across large distances causing a negative impact on carbon emissions. More eco-tourism resulted in more air travel and bigger airports. In other industries scaling had a more positive impact. Towns were major polluters, so more ecological buildings had a positive impact.

scholarly journals Rapid Evaporative Ionization Mass Spectrometry: A Review on Its Application to the Red Meat Industry with an Australian Context

Metabolites ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 171
Author(s):  
Robert S. Barlow ◽  
Adam G. Fitzgerald ◽  
Joanne M. Hughes ◽  
Kate E. McMillan ◽  
Sean C. Moore ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Supply Chain ◽  
Ex Vivo ◽  
Meat Products ◽  
Red Meat ◽  
Ionization Mass ◽  
Meat Industry ◽  
Quality And Safety ◽  
Australian Context ◽  
Processing Plants

The red meat supply chain is a complex network transferring product from producers to consumers in a safe and secure way. There can be times when fragmentation can arise within the supply chain, which could be exploited. This risk needs reduction so that meat products enter the market with the desired attributes. Rapid Evaporative Ionisation Mass Spectrometry (REIMS) is a novel ambient mass spectrometry technique originally developed for rapid and accurate classification of biological tissue which is now being considered for use in a range of additional applications. It has subsequently shown promise for a range of food provenance, quality and safety applications with its ability to conduct ex vivo and in situ analysis. These are regarded as critical characteristics for technologies which can enable real-time decision making in meat processing plants and more broadly throughout the sector. This review presents an overview of the REIMS technology, and its application to the areas of provenance, quality and safety to the red meat industry, particularly in an Australian context.

scholarly journals Automated 16-Plex Plasma Proteomics with Real-Time Search and Ion Mobility Mass Spectrometry Enables Large-Scale Profiling in Naked Mole-Rats and Mice

2021 ◽  
Vol 20 (2) ◽  
pp. 1280-1295
Author(s):  
Aleksandr Gaun ◽  
Kaitlyn N. Lewis Hardell ◽  
Niclas Olsson ◽  
Jonathon J. O’Brien ◽  
Sudha Gollapudi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Real Time ◽  
Ion Mobility ◽  
Large Scale ◽  
Ion Mobility Mass Spectrometry ◽  
Plasma Proteomics ◽  
Rats And Mice
Sign in / Sign up

Export Citation Format

Share Document